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Protein

Nucleoside-specific channel-forming protein tsx

Gene

tsx

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Constitutes the receptor for colicin K and phage T6, and functions as substrate-specific channel for nucleosides and deoxynucleosides.

GO - Molecular functioni

  • nucleoside-specific channel forming porin activity Source: EcoCyc

GO - Biological processi

  • ion transport Source: UniProtKB-KW
  • nucleoside transport Source: EcoCyc
  • viral entry into host cell Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11035-MONOMER.
ECOL316407:JW0401-MONOMER.
MetaCyc:EG11035-MONOMER.

Protein family/group databases

TCDBi1.B.10.1.1. the nucleoside-specific channel-forming outer membrane porin (tsx) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside-specific channel-forming protein tsx
Gene namesi
Name:tsx
Synonyms:nupA
Ordered Locus Names:b0411, JW0401
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11035. tsx.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • pore complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002519023 – 294Nucleoside-specific channel-forming protein tsxAdd BLAST272

Proteomic databases

EPDiP0A927.
PaxDbiP0A927.
PRIDEiP0A927.

Interactioni

Protein-protein interaction databases

BioGridi4263182. 125 interactors.
STRINGi511145.b0411.

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni32 – 34Combined sources3
Beta strandi35 – 47Combined sources13
Beta strandi49 – 52Combined sources4
Beta strandi57 – 67Combined sources11
Beta strandi70 – 79Combined sources10
Beta strandi99 – 107Combined sources9
Helixi108 – 112Combined sources5
Beta strandi121 – 133Combined sources13
Beta strandi136 – 140Combined sources5
Beta strandi142 – 153Combined sources12
Beta strandi156 – 173Combined sources18
Beta strandi180 – 198Combined sources19
Beta strandi201 – 213Combined sources13
Turni217 – 219Combined sources3
Beta strandi227 – 231Combined sources5
Beta strandi235 – 245Combined sources11
Beta strandi247 – 260Combined sources14
Helixi261 – 263Combined sources3
Beta strandi269 – 274Combined sources6
Beta strandi276 – 278Combined sources3
Beta strandi282 – 294Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TLWX-ray3.10A/B23-294[»]
1TLYX-ray3.01A/B23-294[»]
1TLZX-ray3.10A/B23-294[»]
ProteinModelPortaliP0A927.
SMRiP0A927.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A927.

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105DRB. Bacteria.
COG3248. LUCA.
HOGENOMiHOG000273630.
KOiK05517.
OMAiKSTGWGY.

Family and domain databases

Gene3Di2.40.230.20. 1 hit.
InterProiIPR003055. Channel_Tsx.
IPR018013. Channel_Tsx-like.
[Graphical view]
PfamiPF03502. Channel_Tsx. 1 hit.
[Graphical view]
PRINTSiPR01277. CHANNELTSX.
SUPFAMiSSF111364. SSF111364. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A927-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTLLAAGA VLALSSSFTV NAAENDKPQY LSDWWHQSVN VVGSYHTRFG
60 70 80 90 100
PQIRNDTYLE YEAFAKKDWF DFYGYADAPV FFGGNSDAKG IWNHGSPLFM
110 120 130 140 150
EIEPRFSIDK LTNTDLSFGP FKEWYFANNY IYDMGRNKDG RQSTWYMGLG
160 170 180 190 200
TDIDTGLPMS LSMNVYAKYQ WQNYGAANEN EWDGYRFKIK YFVPITDLWG
210 220 230 240 250
GQLSYIGFTN FDWGSDLGDD SGNAINGIKT RTNNSIASSH ILALNYDHWH
260 270 280 290
YSVVARYWHD GGQWNDDAEL NFGNGNFNVR STGWGGYLVV GYNF
Length:294
Mass (Da):33,589
Last modified:July 19, 2005 - v1
Checksum:iAB801DC563552A5C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti276N → Y in phage resistant mutant. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57685 Genomic DNA. Translation: AAA24701.1.
U82664 Genomic DNA. Translation: AAB40167.1.
U00096 Genomic DNA. Translation: AAC73514.1.
AP009048 Genomic DNA. Translation: BAE76191.1.
PIRiJQ0798.
RefSeqiNP_414945.1. NC_000913.3.
WP_001295328.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73514; AAC73514; b0411.
BAE76191; BAE76191; BAE76191.
GeneIDi946242.
KEGGiecj:JW0401.
eco:b0411.
PATRICi32115971. VBIEscCol129921_0427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57685 Genomic DNA. Translation: AAA24701.1.
U82664 Genomic DNA. Translation: AAB40167.1.
U00096 Genomic DNA. Translation: AAC73514.1.
AP009048 Genomic DNA. Translation: BAE76191.1.
PIRiJQ0798.
RefSeqiNP_414945.1. NC_000913.3.
WP_001295328.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TLWX-ray3.10A/B23-294[»]
1TLYX-ray3.01A/B23-294[»]
1TLZX-ray3.10A/B23-294[»]
ProteinModelPortaliP0A927.
SMRiP0A927.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263182. 125 interactors.
STRINGi511145.b0411.

Protein family/group databases

TCDBi1.B.10.1.1. the nucleoside-specific channel-forming outer membrane porin (tsx) family.

Proteomic databases

EPDiP0A927.
PaxDbiP0A927.
PRIDEiP0A927.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73514; AAC73514; b0411.
BAE76191; BAE76191; BAE76191.
GeneIDi946242.
KEGGiecj:JW0401.
eco:b0411.
PATRICi32115971. VBIEscCol129921_0427.

Organism-specific databases

EchoBASEiEB1028.
EcoGeneiEG11035. tsx.

Phylogenomic databases

eggNOGiENOG4105DRB. Bacteria.
COG3248. LUCA.
HOGENOMiHOG000273630.
KOiK05517.
OMAiKSTGWGY.

Enzyme and pathway databases

BioCyciEcoCyc:EG11035-MONOMER.
ECOL316407:JW0401-MONOMER.
MetaCyc:EG11035-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A927.
PROiP0A927.

Family and domain databases

Gene3Di2.40.230.20. 1 hit.
InterProiIPR003055. Channel_Tsx.
IPR018013. Channel_Tsx-like.
[Graphical view]
PfamiPF03502. Channel_Tsx. 1 hit.
[Graphical view]
PRINTSiPR01277. CHANNELTSX.
SUPFAMiSSF111364. SSF111364. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTSX_ECOLI
AccessioniPrimary (citable) accession number: P0A927
Secondary accession number(s): P22786, Q2MC15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.