P0A927 (TSX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside-specific channel-forming protein tsx

Gene names

Name:	tsx
Synonyms:	nupA
Ordered Locus Names:	b0411, JW0401

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constitutes the receptor for colicin K and phage T6, and functions as substrate-specific channel for nucleosides and deoxynucleosides.
Subcellular location	Cell outer membrane; Multi-pass membrane protein.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal Transmembrane Transmembrane beta strand
Molecular function	Porin
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW pore complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleoside transmembrane transporter activity Inferred from electronic annotation. Source: InterPro porin activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Potential

Chain

23 – 294

272

Nucleoside-specific channel-forming protein tsx

PRO_0000025190

Natural variations

Natural variant

276

1

N → Y in phage resistant mutant.

Secondary structure

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294

Helix Strand Turn

Details...

Turn

32 – 34

3

Beta strand

36 – 47

12

Beta strand

49 – 52

4

Beta strand

57 – 67

11

Beta strand

70 – 79

10

Beta strand

99 – 107

9

Helix

108 – 112

5

Beta strand

121 – 133

13

Beta strand

136 – 138

3

Beta strand

142 – 153

12

Beta strand

156 – 173

18

Beta strand

180 – 198

19

Beta strand

201 – 213

13

Turn

217 – 219

3

Beta strand

227 – 231

5

Beta strand

235 – 245

11

Beta strand

247 – 260

14

Helix

261 – 263

3

Beta strand

272 – 274

3

Sequences

	Sequence		Length	Mass (Da)
	P0A927 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: AB801DC563552A5C	FASTA	294	33,589
	10 20 30 40 50 60 MKKTLLAAGA VLALSSSFTV NAAENDKPQY LSDWWHQSVN VVGSYHTRFG PQIRNDTYLE 70 80 90 100 110 120 YEAFAKKDWF DFYGYADAPV FFGGNSDAKG IWNHGSPLFM EIEPRFSIDK LTNTDLSFGP 130 140 150 160 170 180 FKEWYFANNY IYDMGRNKDG RQSTWYMGLG TDIDTGLPMS LSMNVYAKYQ WQNYGAANEN 190 200 210 220 230 240 EWDGYRFKIK YFVPITDLWG GQLSYIGFTN FDWGSDLGDD SGNAINGIKT RTNNSIASSH 250 260 270 280 290 ILALNYDHWH YSVVARYWHD GGQWNDDAEL NFGNGNFNVR STGWGGYLVV GYNF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the tsx gene, which encodes a nucleoside-specific channel-forming protein (Tsx) in the outer membrane of Escherichia coli." Bremer E., Middendorf A., Martinussen J., Valentin-Hansen P. Gene 96:59-65(1990) [PubMed: 2265760] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Analysis of a mutated phage T6 receptor protein of Escherichia coli K 12." Maier C., Middendorf A., Bremer E. Mol. Gen. Genet. 221:491-494(1990) [PubMed: 2199819] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 269-284, PHAGE RESISTANT MUTANT. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M57685 Genomic DNA. Translation: AAA24701.1.
U82664 Genomic DNA. Translation: AAB40167.1.
U00096 Genomic DNA. Translation: AAC73514.1.
AP009048 Genomic DNA. Translation: BAE76191.1.

PIR

JQ0798.

RefSeq

NP_414945.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TLW	X-ray	3.10	A/B	23-294	[»]
1TLY	X-ray	3.01	A/B	23-294	[»]
1TLZ	X-ray	3.10	A/B	23-294	[»]

ProteinModelPortal

P0A927.

SMR

P0A927. Positions 31-294.

ModBase

Search...

Protein family/group databases

TCDB

1.B.10.1.1. nucleoside-specific channel-forming outer membrane porin (Tsx) family.

Proteomic databases

PRIDE

P0A927.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000381; EBESCP00000000381; EBESCG00000000314.
EBESCT00000000382; EBESCP00000000382; EBESCG00000000314.
EBESCT00000015094; EBESCP00000014385; EBESCG00000014154.

GeneID

946242.

GenomeReviews

Gene locus JW0401 in contig AP009048_GR.
Gene locus b0411 in contig U00096_GR.

KEGG

ecj:JW0401.
eco:b0411.

PATRIC

32115971. VBIEscCol129921_0427.

Organism-specific databases

EchoBASE

EB1028.

EcoGene

EG11035. tsx.

Phylogenomic databases

eggNOG

COG3248.

GeneTree

EBGT00050000011554.

HOGENOM

HBG416540.

OMA

FKEWYVA.

ProtClustDB

PRK15106.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11035-MONOMER.

Gene expression databases

Genevestigator

P0A927.

Family and domain databases

InterPro

IPR003055. Channel_Tsx.
IPR018013. Channel_Tsx-like.
[Graphical view]

KO

K05517.

Pfam

PF03502. Channel_Tsx. 1 hit.
[Graphical view]

PRINTS

PR01277. CHANNELTSX.

ProtoNet

Search...

Entry information

Entry name

TSX_ECOLI

Accession

Primary (citable) accession number: P0A927
Secondary accession number(s): P22786, Q2MC15

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program