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Protein

Phosphatidylglycerophosphatase B

Gene

pgpB

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Also has undecaprenyl pyrophosphate phosphatase activity, required for the biosynthesis of the lipid carrier undecaprenyl phosphate. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. The pattern of activities varies according to subcellular location, PGP phosphatase activity is higher in the cytoplasmic membrane, whereas PA and LPA phosphatase activities are higher in the outer membrane. Activity is independent of a divalent cation ion and insensitive to inhibition by N-ethylmaleimide (By similarity).By similarity

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.
1,2-diacyl-sn-glycerol 3-diphosphate + H2O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate.
A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.
Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate.

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
  2. Phosphatidylglycerophosphatase A (pgpA), Phosphatidylglycerophosphatase B (pgpB)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism

Enzyme and pathway databases

BioCyciECOO157:PGPB-MONOMER.
UniPathwayiUPA00084; UER00504.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase B (EC:3.1.3.27)
Alternative name(s):
Diacylglycerol pyrophosphate phosphatase (EC:3.1.3.81)
Short name:
DGPP phosphatase
Phosphatidate phosphatase (EC:3.1.3.4)
Undecaprenyl pyrophosphate phosphatase (EC:3.6.1.27)
Undecaprenyl-diphosphatase
Gene namesi
Name:pgpB
Ordered Locus Names:Z2529, ECs1851
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicBy similarity8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 47PeriplasmicBy similarityAdd BLAST18
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 71CytoplasmicBy similarity3
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Topological domaini93 – 158PeriplasmicBy similarityAdd BLAST66
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 184CytoplasmicBy similarity5
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Topological domaini206 – 210PeriplasmicBy similarity5
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 254CytoplasmicBy similarityAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583611 – 254Phosphatidylglycerophosphatase BAdd BLAST254

Post-translational modificationi

The N-terminus is blocked.By similarity

Interactioni

Protein-protein interaction databases

STRINGi155864.Z2529.

Structurei

3D structure databases

ProteinModelPortaliP0A925.
SMRiP0A925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 105Phosphatase sequence motif I9
Regioni160 – 163Phosphatase sequence motif II4
Regioni200 – 211Phosphatase sequence motif IIIAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EYK. Bacteria.
COG0671. LUCA.
HOGENOMiHOG000286191.
KOiK01096.
OMAiAQRLCGP.

Family and domain databases

Gene3Di1.20.144.10. 2 hits.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 2 hits.

Sequencei

Sequence statusi: Complete.

P0A925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSIARRTAV GAALLLVMPV AVWISGWRWQ PGEQSWLLKA AFWVTETVTQ
60 70 80 90 100
PWGVITHLIL FGWFLWCLRF RIKAAFVLFA ILAAAILVGQ GVKSWIKDKV
110 120 130 140 150
QEPRPFVIWL EKTHHIPVDE FYTLKRAERG NLVKEQLAEE KNIPQYLRSH
160 170 180 190 200
WQKETGFAFP SGHTMFAASW ALLAVGLLWP RRRTLTIAIL LVWATGVMGS
210 220 230 240 250
RLLLGMHWPR DLVVATLISW ALVAVATWLA QRICGPLTPP AEENREIAQR

EQES
Length:254
Mass (Da):29,021
Last modified:July 19, 2005 - v1
Checksum:i9F4E6E88C34D458C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56535.1.
BA000007 Genomic DNA. Translation: BAB35274.1.
PIRiC85759.
C90860.
RefSeqiNP_309878.1. NC_002695.1.
WP_001256538.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG56535; AAG56535; Z2529.
BAB35274; BAB35274; BAB35274.
GeneIDi912812.
KEGGiece:Z2529.
ecs:ECs1851.
PATRICi18353695. VBIEscCol44059_2064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56535.1.
BA000007 Genomic DNA. Translation: BAB35274.1.
PIRiC85759.
C90860.
RefSeqiNP_309878.1. NC_002695.1.
WP_001256538.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0A925.
SMRiP0A925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z2529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56535; AAG56535; Z2529.
BAB35274; BAB35274; BAB35274.
GeneIDi912812.
KEGGiece:Z2529.
ecs:ECs1851.
PATRICi18353695. VBIEscCol44059_2064.

Phylogenomic databases

eggNOGiENOG4105EYK. Bacteria.
COG0671. LUCA.
HOGENOMiHOG000286191.
KOiK01096.
OMAiAQRLCGP.

Enzyme and pathway databases

UniPathwayiUPA00084; UER00504.
BioCyciECOO157:PGPB-MONOMER.

Family and domain databases

Gene3Di1.20.144.10. 2 hits.
InterProiIPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPGPB_ECO57
AccessioniPrimary (citable) accession number: P0A925
Secondary accession number(s): P18201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.