ID   PA1_SHIFL               Reviewed;         289 AA.
AC   P0A923; P00631;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 31.
DE   RecName: Full=Phospholipase A1;
DE            EC=3.1.1.32;
DE            EC=3.1.1.4;
DE   AltName: Full=Detergent-resistant phospholipase A;
DE            Short=DR-phospholipase A;
DE   AltName: Full=Phosphatidylcholine 1-acylhydrolase;
DE   AltName: Full=Outer membrane phospholipase A;
DE            Short=OM PLA;
DE            Short=OMPLA;
DE   Flags: Precursor;
GN   Name=pldA; OrderedLocusNames=SF3899, S3856;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   MEDLINE=22272406; PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H.,
RA   Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J.,
RA   Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S.,
RA   Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y.,
RA   Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   MEDLINE=22590274; PubMed=12704152;
RX   DOI=10.1128/IAI.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W.,
RA   Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A.,
RA   Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S.,
RA   Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella
RT   flexneri serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Hydrolysis of phosphatidylcholine with phospholipase A2
CC       (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Phosphatidylcholine + H(2)O = 2-
CC       acylglycerophosphocholine + a carboxylate.
CC   -!- CATALYTIC ACTIVITY: Phosphatidylcholine + H(2)O = 1-
CC       acylglycerophosphocholine + a carboxylate.
CC   -!- COFACTOR: Calcium (By similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane (By similarity).
CC       Note=One of the very few enzymes located there (By similarity).
CC   -!- SIMILARITY: Belongs to the phospholipase A1 family.
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DR   EMBL; AE005674; AAN45334.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP18864.1; -; Genomic_DNA.
DR   RefSeq; NP_709627.1; -.
DR   RefSeq; NP_839053.1; -.
DR   SMR; P0A923; 28-289.
DR   GeneID; 1023494; -.
DR   GeneID; 1080073; -.
DR   GenomeReviews; AE005674_GR; SF3899.
DR   GenomeReviews; AE014073_GR; S3856.
DR   KEGG; sfl:SF3899; -.
DR   KEGG; sfx:S3856; -.
DR   HOGENOM; P0A923; -.
DR   OMA; P0A923; YTRLMAQ.
DR   BioCyc; SFLE198214:AAN45334.1-MON; -.
DR   BRENDA; 3.1.1.32; 189495.
DR   BRENDA; 3.1.1.4; 189495.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008970; F:phospholipase A1 activity; IEA:EC.
DR   GO; GO:0004623; F:phospholipase A2 activity; IEA:EC.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003187; PLA1.
DR   Gene3D; G3DSA:2.40.230.10; PLA1; 1.
DR   Pfam; PF02253; PLA1; 1.
DR   PRINTS; PR01486; PHPHLIPASEA1.
PE   3: Inferred from homology;
KW   Calcium; Cell membrane; Cell outer membrane; Complete proteome;
KW   Hydrolase; Lipid degradation; Membrane; Signal.
FT   SIGNAL        1     20       By similarity.
FT   CHAIN        21    289       Phospholipase A1.
FT                                /FTId=PRO_0000021985.
FT   ACT_SITE    164    164       By similarity.
SQ   SEQUENCE   289 AA;  33163 MW;  A688AD32AA60F218 CRC64;
     MRTLQGWLLP VFMLPMAVYA QEATVKEVHD APAVRGSIIA NMLQEHDNPF TLYPYDTNYL
     IYTQTSDLNK EAIASYDWAE NARKDEVKFQ LSLAFPLWRG ILGPNSVLGA SYTQKSWWQL
     SNSEESSPFR ETNYEPQLFL GFATDYRFAG WTLRDVEMGY NHDSNGRSDP TSRSWNRLYT
     RLMAENGNWL VEVKPWYVVG NTDDNPDITK YMGYYQLKIG YHLGDAVLSA KGQYNWNTGY
     GGAELGLSYP ITKHVRLYTQ VYSGYGESLI DYNFNQTRVG VGVMLNDLF
//