ID   PA1_ECO57               Reviewed;         289 AA.
AC   P0A922; P00631;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=Phospholipase A1;
DE            EC=3.1.1.32;
DE            EC=3.1.1.4;
DE   AltName: Full=Detergent-resistant phospholipase A;
DE            Short=DR-phospholipase A;
DE   AltName: Full=Phosphatidylcholine 1-acylhydrolase;
DE   AltName: Full=Outer membrane phospholipase A;
DE            Short=OM PLA;
DE            Short=OMPLA;
DE   Flags: Precursor;
GN   Name=pldA; OrderedLocusNames=Z5342, ECs4751;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   MEDLINE=21074935; PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   MEDLINE=21156231; PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Hydrolysis of phosphatidylcholine with phospholipase A2
CC       (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Phosphatidylcholine + H(2)O = 2-
CC       acylglycerophosphocholine + a carboxylate.
CC   -!- CATALYTIC ACTIVITY: Phosphatidylcholine + H(2)O = 1-
CC       acylglycerophosphocholine + a carboxylate.
CC   -!- COFACTOR: Calcium (By similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane. Note=One of the very
CC       few enzymes located there (By similarity).
CC   -!- SIMILARITY: Belongs to the phospholipase A1 family.
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DR   EMBL; AE005174; AAG59017.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB38174.1; -; Genomic_DNA.
DR   PIR; E86069; E86069.
DR   PIR; G91222; G91222.
DR   RefSeq; NP_290453.1; -.
DR   RefSeq; NP_312778.1; -.
DR   SMR; P0A922; 28-289.
DR   GeneID; 915153; -.
DR   GeneID; 960338; -.
DR   GenomeReviews; AE005174_GR; Z5342.
DR   GenomeReviews; BA000007_GR; ECs4751.
DR   KEGG; ece:Z5342; -.
DR   KEGG; ecs:ECs4751; -.
DR   HOGENOM; P0A922; -.
DR   OMA; P0A922; YTRLMAQ.
DR   BioCyc; ECOL83334:ECS4751-MON; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008970; F:phospholipase A1 activity; IEA:EC.
DR   GO; GO:0004623; F:phospholipase A2 activity; IEA:EC.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003187; PLA1.
DR   Gene3D; G3DSA:2.40.230.10; PLA1; 1.
DR   Pfam; PF02253; PLA1; 1.
DR   PRINTS; PR01486; PHPHLIPASEA1.
PE   3: Inferred from homology;
KW   Calcium; Cell membrane; Cell outer membrane; Complete proteome;
KW   Hydrolase; Lipid degradation; Membrane; Signal.
FT   SIGNAL        1     20       By similarity.
FT   CHAIN        21    289       Phospholipase A1.
FT                                /FTId=PRO_0000021984.
FT   ACT_SITE    164    164       By similarity.
SQ   SEQUENCE   289 AA;  33163 MW;  A688AD32AA60F218 CRC64;
     MRTLQGWLLP VFMLPMAVYA QEATVKEVHD APAVRGSIIA NMLQEHDNPF TLYPYDTNYL
     IYTQTSDLNK EAIASYDWAE NARKDEVKFQ LSLAFPLWRG ILGPNSVLGA SYTQKSWWQL
     SNSEESSPFR ETNYEPQLFL GFATDYRFAG WTLRDVEMGY NHDSNGRSDP TSRSWNRLYT
     RLMAENGNWL VEVKPWYVVG NTDDNPDITK YMGYYQLKIG YHLGDAVLSA KGQYNWNTGY
     GGAELGLSYP ITKHVRLYTQ VYSGYGESLI DYNFNQTRVG VGVMLNDLF
//