Reviewed, UniProtKB/Swiss-Prot P0A922 (PA1_ECO57)

Names and origin

Protein names

Recommended name:
    Phospholipase A1
    EC=3.1.1.32
    EC=3.1.1.4
Alternative name(s):
    Detergent-resistant phospholipase A
      Short name=DR-phospholipase A
    Phosphatidylcholine 1-acylhydrolase
    Outer membrane phospholipase A
      Short name=OM PLA
      Short name=OMPLA

Gene names

Name:	pldA
Ordered Locus Names:	Z5342, ECs4751

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Calcium By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell outer membrane. Note: One of the very few enzymes located there By similarity.
Sequence similarities	Belongs to the phospholipase A1 family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Ligand	Calcium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A1 activity Inferred from electronic annotation. Source: EC phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 289	269	Phospholipase A1		PRO_0000021984
Sites
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A922-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: A688AD32AA60F218	FASTA	289	33,163
	10 20 30 40 50 60 MRTLQGWLLP VFMLPMAVYA QEATVKEVHD APAVRGSIIA NMLQEHDNPF TLYPYDTNYL 70 80 90 100 110 120 IYTQTSDLNK EAIASYDWAE NARKDEVKFQ LSLAFPLWRG ILGPNSVLGA SYTQKSWWQL 130 140 150 160 170 180 SNSEESSPFR ETNYEPQLFL GFATDYRFAG WTLRDVEMGY NHDSNGRSDP TSRSWNRLYT 190 200 210 220 230 240 RLMAENGNWL VEVKPWYVVG NTDDNPDITK YMGYYQLKIG YHLGDAVLSA KGQYNWNTGY 250 260 270 280 GGAELGLSYP ITKHVRLYTQ VYSGYGESLI DYNFNQTRVG VGVMLNDLF

References

[1]	"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]	"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG59017.1. BA000007 Genomic DNA. Translation: BAB38174.1.
PIR	E86069. G91222.
RefSeq	NP_290453.1. NP_312778.1.
3D structure databases
SMR	P0A922. Positions 28-289.
ModBase	Search...
Genome annotation databases
GeneID	915153. 960338.
GenomeReviews	Gene locus Z5342 in contig AE005174_GR. Gene locus ECs4751 in contig BA000007_GR.
KEGG	ece:Z5342. ecs:ECs4751.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A922.
OMA	P0A922. YTRLMAQ.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4751-MON.
Family and domain databases
InterPro	IPR003187. PLA1. [Graphical view]
Gene3D	G3DSA:2.40.230.10. PLA1. 1 hit.
Pfam	PF02253. PLA1. 1 hit. [Graphical view]
PRINTS	PR01486. PHPHLIPASEA1.
ProtoNet	Search...

Entry information

Entry name

PA1_ECO57

Accession

Primary (citable) accession number: P0A922
Secondary accession number(s): P00631

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)