ID   OMPX_ECOLI              Reviewed;         171 AA.
AC   P0A917; P36546;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   24-JAN-2024, entry version 135.
DE   RecName: Full=Outer membrane protein X;
DE   Flags: Precursor;
GN   Name=ompX; Synonyms=ybiG; OrderedLocusNames=b0814, JW0799;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7836315; DOI=10.1128/jb.177.3.799-804.1995;
RA   Mecsas J., Welch R., Erickson J.W., Gross C.A.;
RT   "Identification and characterization of an outer membrane protein, OmpX, in
RT   Escherichia coli that is homologous to a family of outer membrane proteins
RT   including Ail of Yersinia enterocolitica.";
RL   J. Bacteriol. 177:799-804(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-137.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8021175; DOI=10.1128/jb.176.13.3928-3935.1994;
RA   Lomovskaya O.L., Kidwell J.P., Matin A.;
RT   "Characterization of the sigma 38-dependent expression of a core
RT   Escherichia coli starvation gene, pexB.";
RL   J. Bacteriol. 176:3928-3935(1994).
RN   [6]
RP   PROTEIN SEQUENCE OF 24-28.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9629924; DOI=10.1002/elps.1150190539;
RA   Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M.,
RA   Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A.;
RT   "Extraction of membrane proteins by differential solubilization for
RT   separation using two-dimensional gel electrophoresis.";
RL   Electrophoresis 19:837-844(1998).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 24-171.
RX   PubMed=10545325; DOI=10.1016/s0969-2126(00)80063-5;
RA   Vogt J., Schulz G.E.;
RT   "The structure of the outer membrane protein OmpX from Escherichia coli
RT   reveals possible mechanisms of virulence.";
RL   Structure 7:1301-1309(1999).
CC   -!- INTERACTION:
CC       P0A917; P0AEU7: skp; NbExp=2; IntAct=EBI-552413, EBI-548242;
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the outer membrane OOP (TC 1.B.6) superfamily.
CC       OmpX family. {ECO:0000305}.
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DR   EMBL; L37088; AAA66329.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73901.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35486.1; -; Genomic_DNA.
DR   EMBL; U04242; AAA21856.1; -; Genomic_DNA.
DR   PIR; I55173; I55173.
DR   RefSeq; NP_415335.1; NC_000913.3.
DR   RefSeq; WP_001295296.1; NZ_STEB01000019.1.
DR   PDB; 1ORM; NMR; -; A=24-171.
DR   PDB; 1Q9F; NMR; -; A=24-171.
DR   PDB; 1Q9G; NMR; -; A=24-171.
DR   PDB; 1QJ8; X-ray; 1.90 A; A=24-171.
DR   PDB; 1QJ9; X-ray; 2.10 A; A=24-171.
DR   PDB; 2M06; NMR; -; A=24-171.
DR   PDB; 2M07; NMR; -; A=24-171.
DR   PDB; 2MNH; NMR; -; A=24-171.
DR   PDBsum; 1ORM; -.
DR   PDBsum; 1Q9F; -.
DR   PDBsum; 1Q9G; -.
DR   PDBsum; 1QJ8; -.
DR   PDBsum; 1QJ9; -.
DR   PDBsum; 2M06; -.
DR   PDBsum; 2M07; -.
DR   PDBsum; 2MNH; -.
DR   AlphaFoldDB; P0A917; -.
DR   BMRB; P0A917; -.
DR   SMR; P0A917; -.
DR   BioGRID; 4261010; 284.
DR   DIP; DIP-48016N; -.
DR   IntAct; P0A917; 3.
DR   MINT; P0A917; -.
DR   STRING; 511145.b0814; -.
DR   DrugBank; DB04233; (Hydroxyethyloxy)Tri(Ethyloxy)Octane.
DR   TCDB; 1.B.6.2.1; the ompa-ompf porin (oop) family.
DR   jPOST; P0A917; -.
DR   PaxDb; 511145-b0814; -.
DR   EnsemblBacteria; AAC73901; AAC73901; b0814.
DR   GeneID; 75204928; -.
DR   GeneID; 944967; -.
DR   KEGG; ecj:JW0799; -.
DR   KEGG; eco:b0814; -.
DR   PATRIC; fig|1411691.4.peg.1464; -.
DR   EchoBASE; EB2056; -.
DR   eggNOG; COG3637; Bacteria.
DR   HOGENOM; CLU_099385_1_0_6; -.
DR   InParanoid; P0A917; -.
DR   OMA; RFNDWAS; -.
DR   OrthoDB; 6504170at2; -.
DR   PhylomeDB; P0A917; -.
DR   BioCyc; EcoCyc:EG12117-MONOMER; -.
DR   EvolutionaryTrace; P0A917; -.
DR   PHI-base; PHI:6250; -.
DR   PRO; PR:P0A917; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
DR   GO; GO:0044384; C:host outer membrane; IEA:InterPro.
DR   Gene3D; 2.40.160.20; -; 1.
DR   InterPro; IPR000758; Enterovir_OMP.
DR   InterPro; IPR011250; OMP/PagP_b-brl.
DR   InterPro; IPR027385; OMP_b-brl.
DR   PANTHER; PTHR35892; OUTER MEMBRANE PROTEIN PAGN-RELATED; 1.
DR   PANTHER; PTHR35892:SF3; OUTER MEMBRANE PROTEIN X; 1.
DR   Pfam; PF13505; OMP_b-brl; 1.
DR   PRINTS; PR00316; ENTEROVIROMP.
DR   SUPFAM; SSF56925; OMPA-like; 1.
DR   PROSITE; PS00694; ENT_VIR_OMP_1; 1.
DR   PROSITE; PS00695; ENT_VIR_OMP_2; 1.
DR   SWISS-2DPAGE; P0A917; -.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Direct protein sequencing; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane beta strand.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:9629924"
FT   CHAIN           24..171
FT                   /note="Outer membrane protein X"
FT                   /id="PRO_0000020199"
FT   TOPO_DOM        24..25
FT                   /note="Periplasmic"
FT   TRANSMEM        26..35
FT                   /note="Beta stranded"
FT   TOPO_DOM        36..44
FT                   /note="Extracellular"
FT   TRANSMEM        45..54
FT                   /note="Beta stranded"
FT   TOPO_DOM        55..59
FT                   /note="Periplasmic"
FT   TRANSMEM        60..69
FT                   /note="Beta stranded"
FT   TOPO_DOM        70..85
FT                   /note="Extracellular"
FT   TRANSMEM        86..95
FT                   /note="Beta stranded"
FT   TOPO_DOM        96..99
FT                   /note="Periplasmic"
FT   TRANSMEM        100..109
FT                   /note="Beta stranded"
FT   TOPO_DOM        110..129
FT                   /note="Extracellular"
FT   TRANSMEM        130..139
FT                   /note="Beta stranded"
FT   TOPO_DOM        140..143
FT                   /note="Periplasmic"
FT   TRANSMEM        144..153
FT                   /note="Beta stranded"
FT   TOPO_DOM        154..160
FT                   /note="Extracellular"
FT   TRANSMEM        161..170
FT                   /note="Beta stranded"
FT   TOPO_DOM        171
FT                   /note="Periplasmic"
FT   CONFLICT        10
FT                   /note="L -> M (in Ref. 5; AAA21856)"
FT                   /evidence="ECO:0000305"
FT   STRAND          25..37
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   TURN            38..40
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          41..54
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          55..57
FT                   /evidence="ECO:0007829|PDB:1ORM"
FT   STRAND          59..74
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          75..77
FT                   /evidence="ECO:0007829|PDB:1ORM"
FT   STRAND          80..97
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          100..117
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   TURN            118..120
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          121..138
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          141..143
FT                   /evidence="ECO:0007829|PDB:1ORM"
FT   STRAND          144..155
FT                   /evidence="ECO:0007829|PDB:1QJ8"
FT   STRAND          158..170
FT                   /evidence="ECO:0007829|PDB:1QJ8"
SQ   SEQUENCE   171 AA;  18603 MW;  77CE1245DAE81462 CRC64;
     MKKIACLSAL AAVLAFTAGT SVAATSTVTG GYAQSDAQGQ MNKMGGFNLK YRYEEDNSPL
     GVIGSFTYTE KSRTASSGDY NKNQYYGITA GPAYRINDWA SIYGVVGVGY GKFQTTEYPT
     YKHDTSDYGF SYGAGLQFNP MENVALDFSY EQSRIRSVDV GTWIAGVGYR F
//