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Protein

Peptidoglycan-associated lipoprotein

Gene

pal

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:11115123, PubMed:17233825). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726).3 Publications

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: EcoCyc

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG10684-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-associated lipoprotein1 PublicationUniRule annotation
Short name:
PALUniRule annotationCurated
Alternative name(s):
Tol-Pal system lipoprotein PalCurated
Gene namesi
Name:pal1 PublicationUniRule annotation
Synonyms:excC1 Publication
Ordered Locus Names:b0741, JW0731
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10684 pal

Subcellular locationi

  • Cell outer membrane UniRule annotation1 Publication; Lipid-anchor UniRule annotation1 Publication
  • Note: Accumulates at cell constriction sites. Recruitment to the division site is dependent on FtsN activity.1 Publication

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: InterPro
  • intrinsic component of periplasmic side of cell outer membrane Source: EcoCyc

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotationAdd BLAST21
ChainiPRO_000002012022 – 173Peptidoglycan-associated lipoproteinUniRule annotationAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi22N-palmitoyl cysteineUniRule annotation1 Publication1
Lipidationi22S-diacylglycerol cysteineUniRule annotation1 Publication1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0A912
PaxDbiP0A912
PRIDEiP0A912

Interactioni

Subunit structurei

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). Pal interacts with TolB (PubMed:7744736, PubMed:17375930, PubMed:19696740). Interacts with the C-terminal domain of TolA. This interaction is proton motive force dependent and requires TolQ and TolR (PubMed:11115123, PubMed:11722743). Can form a complex with TolB and OmpA (PubMed:9701827).1 Publication6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260724, 260 interactors
DIPiDIP-51001N
IntActiP0A912, 8 interactors
MINTiP0A912
STRINGi316385.ECDH10B_0808

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi69 – 72Combined sources4
Helixi82 – 84Combined sources3
Helixi85 – 97Combined sources13
Beta strandi103 – 107Combined sources5
Beta strandi111 – 113Combined sources3
Helixi115 – 135Combined sources21
Helixi140 – 142Combined sources3
Beta strandi143 – 147Combined sources5
Helixi160 – 166Combined sources7
Beta strandi167 – 172Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OAPX-ray1.93A65-173[»]
2HQSX-ray1.50C/E/G/H65-173[»]
2W8BX-ray1.86C/E/G/H65-173[»]
ProteinModelPortaliP0A912
SMRiP0A912
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A912

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 173OmpA-likeUniRule annotationAdd BLAST114

Sequence similaritiesi

Belongs to the OmpA family. Pal subfamily.UniRule annotationCurated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105KCK Bacteria
COG2885 LUCA
HOGENOMiHOG000189913
InParanoidiP0A912
KOiK03640
OMAiGSAQDFT
PhylomeDBiP0A912

Family and domain databases

CDDicd07185 OmpA_C-like, 1 hit
Gene3Di3.30.1330.60, 1 hit
HAMAPiMF_02204 Pal, 1 hit
InterProiView protein in InterPro
IPR006664 OMP_bac
IPR006665 OmpA-like
IPR006690 OMPA-like_CS
IPR036737 OmpA-like_sf
IPR039001 Pal
IPR014169 Pal_lipo_C
PfamiView protein in Pfam
PF00691 OmpA, 1 hit
PRINTSiPR01021 OMPADOMAIN
SUPFAMiSSF103088 SSF103088, 1 hit
TIGRFAMsiTIGR02802 Pal_lipo, 1 hit
PROSITEiView protein in PROSITE
PS01068 OMPA_1, 1 hit
PS51123 OMPA_2, 1 hit
PS51257 PROKAR_LIPOPROTEIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A912-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLNKVLKGL MIALPVMAIA ACSSNKNASN DGSEGMLGAG TGMDANGGNG
60 70 80 90 100
NMSSEEQARL QMQQLQQNNI VYFDLDKYDI RSDFAQMLDA HANFLRSNPS
110 120 130 140 150
YKVTVEGHAD ERGTPEYNIS LGERRANAVK MYLQGKGVSA DQISIVSYGK
160 170
EKPAVLGHDE AAYSKNRRAV LVY
Length:173
Mass (Da):18,824
Last modified:April 1, 1988 - v1
Checksum:i449F9959C0274430
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05123 Genomic DNA Translation: CAA28771.1
X65796 Genomic DNA Translation: CAA46673.1
U00096 Genomic DNA Translation: AAC73835.1
AP009048 Genomic DNA Translation: BAA35407.1
M28232 Genomic DNA No translation available.
PIRiA27534 LPECPG
RefSeqiNP_415269.1, NC_000913.3
WP_001295306.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73835; AAC73835; b0741
BAA35407; BAA35407; BAA35407
GeneIDi945004
KEGGiecj:JW0731
eco:b0741
PATRICifig|1411691.4.peg.1531

Similar proteinsi

Entry informationi

Entry nameiPAL_ECOLI
AccessioniPrimary (citable) accession number: P0A912
Secondary accession number(s): P07176
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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