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Protein

Outer membrane protein A

Gene

ompA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the action of colicins K and L and for the stabilization of mating aggregates in conjugation. Serves as a receptor for a number of T-even like phages. Also acts as a porin with low permeability that allows slow penetration of small solutes.

GO - Molecular functioni

  • porin activity Source: EcoCyc
  • structural molecule activity Source: InterPro

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • conjugation Source: EcoliWiki
  • detection of virus Source: EcoliWiki
  • ion transmembrane transport Source: EcoCyc
  • ion transport Source: EcoliWiki
  • transport Source: EcoliWiki
  • viral entry into host cell Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Conjugation, Ion transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG10669-MONOMER.
ECOL316407:JW0940-MONOMER.

Protein family/group databases

TCDBi1.B.6.1.1. the ompa-ompf porin (oop) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein A
Alternative name(s):
Outer membrane protein II*
Gene namesi
Name:ompA
Synonyms:con, tolG, tut
Ordered Locus Names:b0957, JW0940
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10669. ompA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 26Periplasmic5
Transmembranei27 – 37Beta strandedAdd BLAST11
Topological domaini38 – 54ExtracellularAdd BLAST17
Transmembranei55 – 66Beta strandedAdd BLAST12
Topological domaini67 – 69Periplasmic3
Transmembranei70 – 78Beta stranded9
Topological domaini79 – 95ExtracellularAdd BLAST17
Transmembranei96 – 107Beta strandedAdd BLAST12
Topological domaini108 – 111Periplasmic4
Transmembranei112 – 124Beta strandedAdd BLAST13
Topological domaini125 – 137ExtracellularAdd BLAST13
Transmembranei138 – 151Beta strandedAdd BLAST14
Topological domaini152 – 155Periplasmic4
Transmembranei156 – 163Beta stranded8
Topological domaini164 – 181ExtracellularAdd BLAST18
Transmembranei182 – 190Beta stranded9
Topological domaini191 – 346PeriplasmicAdd BLAST156

GO - Cellular componenti

  • cell outer membrane Source: EcoliWiki
  • integral component of membrane Source: EcoliWiki
  • membrane Source: EcoliWiki
  • outer membrane Source: EcoliWiki
  • pore complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 214 PublicationsAdd BLAST21
ChainiPRO_000002009422 – 346Outer membrane protein AAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi311 ↔ 323

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0A910.
PaxDbiP0A910.
PRIDEiP0A910.

2D gel databases

SWISS-2DPAGEP0A910.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
degPP0C0V06EBI-371347,EBI-547165
rcsFP694113EBI-371347,EBI-1114706
tigP0A8503EBI-371347,EBI-544862

Protein-protein interaction databases

BioGridi4260032. 1011 interactors.
DIPiDIP-31879N.
IntActiP0A910. 13 interactors.
MINTiMINT-1308131.
STRINGi511145.b0957.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 37Combined sources11
Beta strandi41 – 43Combined sources3
Beta strandi46 – 48Combined sources3
Beta strandi50 – 53Combined sources4
Beta strandi55 – 67Combined sources13
Beta strandi70 – 81Combined sources12
Beta strandi93 – 128Combined sources36
Beta strandi130 – 132Combined sources3
Beta strandi134 – 153Combined sources20
Beta strandi156 – 165Combined sources10
Turni172 – 175Combined sources4
Beta strandi182 – 190Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BXWX-ray2.50A21-192[»]
1G90NMR-A22-197[»]
1QJPX-ray1.65A22-192[»]
2GE4NMR-A22-197[»]
2JMMNMR-A23-197[»]
3NB3electron microscopy-A/B/C1-346[»]
ProteinModelPortaliP0A910.
SMRiP0A910.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A910.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati201 – 20212
Repeati203 – 20422
Repeati205 – 20632
Repeati207 – 20842
Domaini210 – 338OmpA-likePROSITE-ProRule annotationAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni197 – 208Hinge-likeAdd BLAST12
Regioni201 – 2084 X 2 AA tandem repeats of A-P8

Sequence similaritiesi

Belongs to the OmpA family.Curated
Contains 1 OmpA-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105UYK. Bacteria.
COG2885. LUCA.
HOGENOMiHOG000274199.
InParanoidiP0A910.
KOiK03286.
OMAiEYALTKN.

Family and domain databases

CDDicd07185. OmpA_C-like. 1 hit.
Gene3Di2.40.160.20. 1 hit.
3.30.1330.60. 1 hit.
InterProiIPR011250. OMP/PagP_b-brl.
IPR006664. OMP_bac.
IPR002368. OmpA.
IPR006665. OmpA-like.
IPR006690. OMPA-like_CS.
IPR000498. OmpA-like_TM_dom.
[Graphical view]
PfamiPF00691. OmpA. 1 hit.
PF01389. OmpA_membrane. 1 hit.
[Graphical view]
PRINTSiPR01021. OMPADOMAIN.
PR01022. OUTRMMBRANEA.
SUPFAMiSSF103088. SSF103088. 1 hit.
SSF56925. SSF56925. 1 hit.
PROSITEiPS01068. OMPA_1. 1 hit.
PS51123. OMPA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTAIAIAV ALAGFATVAQ AAPKDNTWYT GAKLGWSQYH DTGFINNNGP
60 70 80 90 100
THENQLGAGA FGGYQVNPYV GFEMGYDWLG RMPYKGSVEN GAYKAQGVQL
110 120 130 140 150
TAKLGYPITD DLDIYTRLGG MVWRADTKSN VYGKNHDTGV SPVFAGGVEY
160 170 180 190 200
AITPEIATRL EYQWTNNIGD AHTIGTRPDN GMLSLGVSYR FGQGEAAPVV
210 220 230 240 250
APAPAPAPEV QTKHFTLKSD VLFNFNKATL KPEGQAALDQ LYSQLSNLDP
260 270 280 290 300
KDGSVVVLGY TDRIGSDAYN QGLSERRAQS VVDYLISKGI PADKISARGM
310 320 330 340
GESNPVTGNT CDNVKQRAAL IDCLAPDRRV EIEVKGIKDV VTQPQA
Length:346
Mass (Da):37,201
Last modified:July 21, 1986 - v1
Checksum:i195147734CDF8B04
GO

Mass spectrometryi

Molecular mass is 35177 Da from positions 22 - 346. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00307 Genomic DNA. Translation: CAA23588.1.
U00096 Genomic DNA. Translation: AAC74043.1.
AP009048 Genomic DNA. Translation: BAA35715.1.
PIRiA93707. MMECA.
RefSeqiNP_415477.1. NC_000913.3.
WP_000750416.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74043; AAC74043; b0957.
BAA35715; BAA35715; BAA35715.
GeneIDi945571.
KEGGiecj:JW0940.
eco:b0957.
PATRICi32117133. VBIEscCol129921_0991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00307 Genomic DNA. Translation: CAA23588.1.
U00096 Genomic DNA. Translation: AAC74043.1.
AP009048 Genomic DNA. Translation: BAA35715.1.
PIRiA93707. MMECA.
RefSeqiNP_415477.1. NC_000913.3.
WP_000750416.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BXWX-ray2.50A21-192[»]
1G90NMR-A22-197[»]
1QJPX-ray1.65A22-192[»]
2GE4NMR-A22-197[»]
2JMMNMR-A23-197[»]
3NB3electron microscopy-A/B/C1-346[»]
ProteinModelPortaliP0A910.
SMRiP0A910.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260032. 1011 interactors.
DIPiDIP-31879N.
IntActiP0A910. 13 interactors.
MINTiMINT-1308131.
STRINGi511145.b0957.

Protein family/group databases

TCDBi1.B.6.1.1. the ompa-ompf porin (oop) family.

2D gel databases

SWISS-2DPAGEP0A910.

Proteomic databases

EPDiP0A910.
PaxDbiP0A910.
PRIDEiP0A910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74043; AAC74043; b0957.
BAA35715; BAA35715; BAA35715.
GeneIDi945571.
KEGGiecj:JW0940.
eco:b0957.
PATRICi32117133. VBIEscCol129921_0991.

Organism-specific databases

EchoBASEiEB0663.
EcoGeneiEG10669. ompA.

Phylogenomic databases

eggNOGiENOG4105UYK. Bacteria.
COG2885. LUCA.
HOGENOMiHOG000274199.
InParanoidiP0A910.
KOiK03286.
OMAiEYALTKN.

Enzyme and pathway databases

BioCyciEcoCyc:EG10669-MONOMER.
ECOL316407:JW0940-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A910.
PROiP0A910.

Family and domain databases

CDDicd07185. OmpA_C-like. 1 hit.
Gene3Di2.40.160.20. 1 hit.
3.30.1330.60. 1 hit.
InterProiIPR011250. OMP/PagP_b-brl.
IPR006664. OMP_bac.
IPR002368. OmpA.
IPR006665. OmpA-like.
IPR006690. OMPA-like_CS.
IPR000498. OmpA-like_TM_dom.
[Graphical view]
PfamiPF00691. OmpA. 1 hit.
PF01389. OmpA_membrane. 1 hit.
[Graphical view]
PRINTSiPR01021. OMPADOMAIN.
PR01022. OUTRMMBRANEA.
SUPFAMiSSF103088. SSF103088. 1 hit.
SSF56925. SSF56925. 1 hit.
PROSITEiPS01068. OMPA_1. 1 hit.
PS51123. OMPA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOMPA_ECOLI
AccessioniPrimary (citable) accession number: P0A910
Secondary accession number(s): P02934
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.