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Protein

MltA-interacting protein

Gene

mipA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May serve as a scaffold protein required for the formation of a complex with MrcB/PonB and MltA, this complex could play a role in enlargement and septation of the murein sacculus.

GO - Molecular functioni

  • protein binding, bridging Source: EcoCyc

GO - Biological processi

  • peptidoglycan biosynthetic process Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6968-MONOMER.
ECOL316407:JW1771-MONOMER.

Protein family/group databases

TCDBi9.B.99.1.2. the mipa-interacting protein (mipa) family.

Names & Taxonomyi

Protein namesi
Recommended name:
MltA-interacting protein
Gene namesi
Name:mipA
Synonyms:yeaF
Ordered Locus Names:b1782, JW1771
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13492. mipA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 248226MltA-interacting proteinPRO_0000019092Add
BLAST

Proteomic databases

EPDiP0A908.
PaxDbiP0A908.
PRIDEiP0A908.

Interactioni

Subunit structurei

Forms a trimeric complex with MrcB and MltA in vitro.

GO - Molecular functioni

  • protein binding, bridging Source: EcoCyc

Protein-protein interaction databases

BioGridi4260311. 186 interactions.
IntActiP0A908. 4 interactions.
STRINGi511145.b1782.

Structurei

3D structure databases

ProteinModelPortaliP0A908.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MipA/OmpV family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105GNX. Bacteria.
COG3713. LUCA.
HOGENOMiHOG000121141.
InParanoidiP0A908.
KOiK07274.
OMAiLSIMAYY.
OrthoDBiEOG6ZKXQ0.
PhylomeDBiP0A908.

Family and domain databases

InterProiIPR010583. MipA.
[Graphical view]
PfamiPF06629. MipA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLKLLALG VLIATSAGVA HAEGKFSLGA GVGVVEHPYK DYDTDVYPVP
60 70 80 90 100
VINYEGDNFW FRGLGGGYYL WNDATDKLSI TAYWSPLYFK AKDSGDHQMR
110 120 130 140 150
HLDDRKSTMM AGLSYAHFTQ YGYLRTTLAG DTLDNSNGIV WDMAWLYRYT
160 170 180 190 200
NGGLTVTPGI GVQWNSENQN EYYYGVSRKE SARSGLRGYN PNDSWSPYLE
210 220 230 240
LSASYNFLGD WSVYGTARYT RLSDEVTDSP MVDKSWTGLI STGITYKF
Length:248
Mass (Da):27,831
Last modified:July 19, 2005 - v1
Checksum:i6571B0D7A47A0525
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341V → N AA sequence (PubMed:10037771).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74852.1.
AP009048 Genomic DNA. Translation: BAA15579.1.
PIRiF64938.
RefSeqiNP_416296.1. NC_000913.3.
WP_000163771.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74852; AAC74852; b1782.
BAA15579; BAA15579; BAA15579.
GeneIDi946301.
KEGGiecj:JW1771.
eco:b1782.
PATRICi32118875. VBIEscCol129921_1855.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74852.1.
AP009048 Genomic DNA. Translation: BAA15579.1.
PIRiF64938.
RefSeqiNP_416296.1. NC_000913.3.
WP_000163771.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A908.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260311. 186 interactions.
IntActiP0A908. 4 interactions.
STRINGi511145.b1782.

Protein family/group databases

TCDBi9.B.99.1.2. the mipa-interacting protein (mipa) family.

Proteomic databases

EPDiP0A908.
PaxDbiP0A908.
PRIDEiP0A908.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74852; AAC74852; b1782.
BAA15579; BAA15579; BAA15579.
GeneIDi946301.
KEGGiecj:JW1771.
eco:b1782.
PATRICi32118875. VBIEscCol129921_1855.

Organism-specific databases

EchoBASEiEB3265.
EcoGeneiEG13492. mipA.

Phylogenomic databases

eggNOGiENOG4105GNX. Bacteria.
COG3713. LUCA.
HOGENOMiHOG000121141.
InParanoidiP0A908.
KOiK07274.
OMAiLSIMAYY.
OrthoDBiEOG6ZKXQ0.
PhylomeDBiP0A908.

Enzyme and pathway databases

BioCyciEcoCyc:G6968-MONOMER.
ECOL316407:JW1771-MONOMER.

Miscellaneous databases

PROiP0A908.

Family and domain databases

InterProiIPR010583. MipA.
[Graphical view]
PfamiPF06629. MipA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Demonstration of molecular interactions between the murein polymerase PBP1B, the lytic transglycosylase MltA, and the scaffolding protein MipA of Escherichia coli."
    Vollmer W., von Rechenberg M., Hoeltje J.-V.
    J. Biol. Chem. 274:6726-6734(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-35, INTERACTION WITH MLTA AND MRCB/PONB.
    Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: SUBCELLULAR LOCATION.
    Strain: BL21-DE3.

Entry informationi

Entry nameiMIPA_ECOLI
AccessioniPrimary (citable) accession number: P0A908
Secondary accession number(s): O07962, P77486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 16, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.