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Protein

Outer membrane protein assembly factor BamC

Gene

bamC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex that stabilizes the interaction between the essential proteins BamA and BamD.UniRule annotation3 Publications

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoliWiki
  • protein insertion into membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10658-MONOMER.
ECOL316407:JW2462-MONOMER.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein assembly factor BamCUniRule annotation
Gene namesi
Name:bamCUniRule annotation
Synonyms:dapX, nlpB
Ordered Locus Names:b2477, JW2462
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10658. bamC.

Subcellular locationi

GO - Cellular componenti

  • Bam protein complex Source: EcoCyc
  • cell surface Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 344320Outer membrane protein assembly factor BamCPRO_0000018027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi25 – 251N-palmitoyl cysteineUniRule annotation1 Publication
Lipidationi25 – 251S-diacylglycerol cysteineUniRule annotation1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP0A903.
PaxDbiP0A903.
PRIDEiP0A903.

2D gel databases

SWISS-2DPAGEP0A903.

Interactioni

Subunit structurei

Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. Forms a subcomplex with BamD and BamE.UniRule annotation5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1129043,EBI-1129043
bamDP0AC024EBI-1129043,EBI-1128087

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262898. 284 interactions.
IntActiP0A903. 7 interactions.
STRINGi511145.b2477.

Structurei

Secondary structure

1
344
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi30 – 334Combined sources
Turni37 – 393Combined sources
Helixi40 – 434Combined sources
Helixi77 – 793Combined sources
Beta strandi96 – 1005Combined sources
Beta strandi103 – 1086Combined sources
Helixi109 – 1124Combined sources
Helixi116 – 12611Combined sources
Beta strandi131 – 1355Combined sources
Helixi136 – 1383Combined sources
Beta strandi140 – 1434Combined sources
Beta strandi146 – 1483Combined sources
Helixi153 – 1553Combined sources
Beta strandi157 – 16812Combined sources
Beta strandi171 – 18313Combined sources
Beta strandi186 – 1883Combined sources
Helixi191 – 20818Combined sources
Beta strandi230 – 2345Combined sources
Turni235 – 2373Combined sources
Beta strandi240 – 2467Combined sources
Helixi248 – 26114Combined sources
Beta strandi264 – 2707Combined sources
Helixi271 – 2733Combined sources
Beta strandi275 – 2806Combined sources
Helixi285 – 2917Combined sources
Beta strandi300 – 31011Combined sources
Beta strandi313 – 3197Combined sources
Beta strandi321 – 3233Combined sources
Helixi328 – 34417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LAENMR-A101-344[»]
2LAFNMR-A101-344[»]
2YH5X-ray1.25A226-344[»]
2YH6X-ray1.55A/B/C/D101-212[»]
3SNSX-ray1.50A224-343[»]
3TGOX-ray2.90C/D26-344[»]
5AYWX-ray3.56C33-88[»]
5D0OX-ray2.90C1-344[»]
5D0QX-ray3.50C/G1-344[»]
5EKQX-ray3.39C25-344[»]
ProteinModelPortaliP0A903.
SMRiP0A903. Positions 29-216, 226-344.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A903.

Family & Domainsi

Domaini

Contains two well-defined domains connected by a flexible linker. The C-terminal domain may serve as an important protein-binding surface for interaction with other Bam components or substrates. In addition, contains a long unstructured N-terminal region, which is required to stabilize the BamCD complex.3 Publications

Sequence similaritiesi

Belongs to the BamC family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107EG0. Bacteria.
COG3317. LUCA.
HOGENOMiHOG000123040.
KOiK07287.
OMAiQKSMVAK.

Family and domain databases

HAMAPiMF_00924. OM_assembly_BamC. 1 hit.
InterProiIPR014524. BamC.
IPR010653. NlpB/DapX.
[Graphical view]
PfamiPF06804. Lipoprotein_18. 1 hit.
[Graphical view]
PIRSFiPIRSF026343. NlpB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYSVQKSRL AKVAGVSLVL LLAACSSDSR YKRQVSGDEA YLEAAPLAEL
60 70 80 90 100
HAPAGMILPV TSGDYAIPVT NGSGAVGKAL DIRPPAQPLA LVSGARTQFT
110 120 130 140 150
GDTASLLVEN GRGNTLWPQV VSVLQAKNYT ITQRDDAGQT LTTDWVQWNR
160 170 180 190 200
LDEDEQYRGR YQISVKPQGY QQAVTVKLLN LEQAGKPVAD AASMQRYSTE
210 220 230 240 250
MMNVISAGLD KSATDAANAA QNRASTTMDV QSAADDTGLP MLVVRGPFNV
260 270 280 290 300
VWQRLPAALE KVGMKVTDST RSQGNMAVTY KPLSDSDWQE LGASDPGLAS
310 320 330 340
GDYKLQVGDL DNRSSLQFID PKGHTLTQSQ NDALVAVFQA AFSK
Length:344
Mass (Da):36,842
Last modified:July 19, 2005 - v1
Checksum:i49991F277D9D923C
GO

Sequence cautioni

The sequence M33928 differs from that shown. Reason: Frameshift at positions 50, 64, 81 and 134. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191A → R (PubMed:2120198).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57402 Genomic DNA. Translation: CAA40661.1.
M33928 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75530.2.
AP009048 Genomic DNA. Translation: BAA16354.1.
PIRiD65023.
RefSeqiNP_416972.4. NC_000913.3.
WP_001297320.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75530; AAC75530; b2477.
BAA16354; BAA16354; BAA16354.
GeneIDi946954.
KEGGiecj:JW2462.
eco:b2477.
PATRICi32120341. VBIEscCol129921_2573.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57402 Genomic DNA. Translation: CAA40661.1.
M33928 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75530.2.
AP009048 Genomic DNA. Translation: BAA16354.1.
PIRiD65023.
RefSeqiNP_416972.4. NC_000913.3.
WP_001297320.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LAENMR-A101-344[»]
2LAFNMR-A101-344[»]
2YH5X-ray1.25A226-344[»]
2YH6X-ray1.55A/B/C/D101-212[»]
3SNSX-ray1.50A224-343[»]
3TGOX-ray2.90C/D26-344[»]
5AYWX-ray3.56C33-88[»]
5D0OX-ray2.90C1-344[»]
5D0QX-ray3.50C/G1-344[»]
5EKQX-ray3.39C25-344[»]
ProteinModelPortaliP0A903.
SMRiP0A903. Positions 29-216, 226-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262898. 284 interactions.
IntActiP0A903. 7 interactions.
STRINGi511145.b2477.

Protein family/group databases

TCDBi1.B.33.1.3. the outer membrane protein insertion porin (bam complex) (ompip) family.

2D gel databases

SWISS-2DPAGEP0A903.

Proteomic databases

EPDiP0A903.
PaxDbiP0A903.
PRIDEiP0A903.

Protocols and materials databases

DNASUi946954.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75530; AAC75530; b2477.
BAA16354; BAA16354; BAA16354.
GeneIDi946954.
KEGGiecj:JW2462.
eco:b2477.
PATRICi32120341. VBIEscCol129921_2573.

Organism-specific databases

EchoBASEiEB0652.
EcoGeneiEG10658. bamC.

Phylogenomic databases

eggNOGiENOG4107EG0. Bacteria.
COG3317. LUCA.
HOGENOMiHOG000123040.
KOiK07287.
OMAiQKSMVAK.

Enzyme and pathway databases

BioCyciEcoCyc:EG10658-MONOMER.
ECOL316407:JW2462-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A903.
PROiP0A903.

Family and domain databases

HAMAPiMF_00924. OM_assembly_BamC. 1 hit.
InterProiIPR014524. BamC.
IPR010653. NlpB/DapX.
[Graphical view]
PfamiPF06804. Lipoprotein_18. 1 hit.
[Graphical view]
PIRSFiPIRSF026343. NlpB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAMC_ECOLI
AccessioniPrimary (citable) accession number: P0A903
Secondary accession number(s): P21167, P76564
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.