Reviewed, UniProtKB/Swiss-Prot P0A8Y8 (YBDB_ECOLI)

Names and origin

Protein names

Recommended name:
    Esterase ybdB
    EC=3.1.-.-
Alternative name(s):
    p15

Gene names

Name:	ybdB
Ordered Locus Names:	b0597, JW0589

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Displays esterase activity toward palmitoyl-CoA and pNP-butyrate. Ref.6
Sequence similarities	Belongs to the UPF0152 (paaI) family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1118982,EBI-548960

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 137

137

Esterase ybdB

PRO_0000156676

Secondary structure

1

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137

Helix Strand Turn

Details...

Helix

9 – 14

6

Helix

20 – 23

4

Beta strand

27 – 31

5

Beta strand

36 – 41

6

Turn

44 – 46

3

Beta strand

51 – 53

3

Helix

55 – 71

17

Beta strand

80 – 89

10

Beta strand

95 – 107

13

Beta strand

109 – 119

11

Beta strand

125 – 135

11

Sequences

	Sequence		Length	Mass (Da)
	P0A8Y8-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: C8DF8DE63815F206	FASTA	137	14,970
	10 20 30 40 50 60 MIWKRHLTLD ELNATSDNTM VAHLGIVYTR LGDDVLEAEM PVDTRTHQPF GLLHGGASAA 70 80 90 100 110 120 LAETLGSMAG FMMTRDGQCV VGTELNATHH RPVSEGKVRG VCQPLHLGRQ NQSWEIVVFD 130 EQGRRCCTCR LGTAVLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA." Nahlik M.S., Brickman T.J., Ozenberger B.A., McIntosh M.A. J. Bacteriol. 171:784-790(1989) [PubMed: 2521621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase." Liu J., Duncan K., Walsh C.T. J. Bacteriol. 171:791-798(1989) [PubMed: 2521622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed: 15808744] [Abstract] Cited for: FUNCTION.
[7]	"Crystal structure of a putative thioesterase." Murshudov G.N., Vagin A.A., Dodson E.J. Submitted (DEC-2003) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 2-137.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M24148 Unassigned DNA. Translation: AAA16104.1.
M24143 Genomic DNA. Translation: AAA76837.1.
U82598 Genomic DNA. Translation: AAB40797.1.
U00096 Genomic DNA. Translation: AAC73698.1.
AP009048 Genomic DNA. Translation: BAE76352.1.

PIR

Q3ECEA. B91904.

RefSeq

AP_001244.1.
NP_415129.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VH9	X-ray	2.15	A/B	2-137	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A8Y8. 1 interaction.

Genome annotation databases

GeneID

945215.

GenomeReviews

Gene locus JW0589 in contig AP009048_GR.
Gene locus b0597 in contig U00096_GR.

KEGG

ecj:JW0589.
eco:b0597.

Organism-specific databases

EchoBASE

EB1097.

EcoGene

EG11105. ybdB.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A8Y8.

OMA

P0A8Y8. TTHEIVM.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11105-MON.

BRENDA

3.1.2.2. 246.

Family and domain databases

InterPro

IPR003736. PAAI.
IPR006683. Thioestr_supf.
[Graphical view]

Pfam

PF03061. 4HBT. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00369. unchar_dom_1. 1 hit.

ProtoNet

Search...

Entry information

Entry name

YBDB_ECOLI

Accession

Primary (citable) accession number: P0A8Y8
Secondary accession number(s): P15050, Q2MBK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)