P0A8Y5 (YIDA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatase YidA
EC=3.1.3.-

Gene names

Name:	yidA
Ordered Locus Names:	b3697, JW3674

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates erythrose 4-phosphate and mannose 1-phosphate. Ref.5
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. Cof family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	magnesium ion binding Inferred from direct assay. Source: EcoliWiki sugar-phosphatase activity Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 270

270

Phosphatase YidA

PRO_0000054423

Sites

Active site

9

1

Nucleophile By similarity

Metal binding

9

1

Magnesium By similarity

Metal binding

11

1

Magnesium By similarity

Metal binding

220

1

Magnesium By similarity

Secondary structure

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270

Helix Strand Turn

Details...

Beta strand

5 – 8

4

Helix

10 – 14

5

Helix

23 – 34

12

Beta strand

38 – 42

5

Helix

47 – 49

3

Helix

51 – 56

6

Beta strand

65 – 68

4

Helix

69 – 71

3

Beta strand

73 – 76

4

Turn

77 – 79

3

Beta strand

82 – 85

4

Helix

90 – 103

14

Beta strand

106 – 110

5

Beta strand

115 – 117

3

Beta strand

120 – 122

3

Helix

124 – 132

9

Beta strand

137 – 139

3

Helix

142 – 144

3

Beta strand

153 – 157

5

Helix

160 – 169

10

Helix

172 – 177

6

Beta strand

178 – 184

7

Beta strand

187 – 192

6

Helix

197 – 208

12

Helix

212 – 214

3

Beta strand

215 – 219

5

Helix

222 – 224

3

Helix

225 – 230

6

Beta strand

231 – 236

6

Helix

242 – 247

6

Beta strand

249 – 251

3

Turn

255 – 258

4

Helix

259 – 267

9

Sequences

	Sequence		Length	Mass (Da)
	P0A8Y5 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 04E749788D8A9446	FASTA	270	29,721
	10 20 30 40 50 60 MAIKLIAIDM DGTLLLPDHT ISPAVKNAIA AARARGVNVV LTTGRPYAGV HNYLKELHME 70 80 90 100 110 120 QPGDYCITYN GALVQKAADG STVAQTALSY DDYRFLEKLS REVGSHFHAL DRTTLYTANR 130 140 150 160 170 180 DISYYTVHES FVATIPLVFC EAEKMDPNTQ FLKVMMIDEP AILDQAIARI PQEVKEKYTV 190 200 210 220 230 240 LKSAPYFLEI LDKRVNKGTG VKSLADVLGI KPEEIMAIGD QENDIAMIEY AGVGVAMDNA 250 260 270 IPSVKEVANF VTKSNLEDGV AFAIEKYVLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy." Adachi T., Mizuuchi M., Robinson E.A., Appella E., O'Dea M.H., Gellert M., Mizuuchi K. Nucleic Acids Res. 15:771-784(1987) [PubMed: 3029692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-195.
[5]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed: 15808744] [Abstract] Cited for: FUNCTION.
[6]	"Crystal structure of Nysgrc target T1436: a hypothetical protein YidA." Ramagopal U.A., Almo S.C. Submitted (MAR-2004) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 2-270.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L10328 Genomic DNA. Translation: AAA62048.1.
U00096 Genomic DNA. Translation: AAC76720.1.
AP009048 Genomic DNA. Translation: BAE77597.1.
X04341 Genomic DNA. Translation: CAA27873.1.

PIR

QQECGB. B65172.

RefSeq

NP_418152.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RKQ	X-ray	1.40	A/B	2-270	[»]

ProteinModelPortal

P0A8Y5.

SMR

P0A8Y5. Positions 1-270.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36033N.

IntAct

P0A8Y5. 9 interactions.

MINT

MINT-1238706.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002453; EBESCP00000002453; EBESCG00000002000.
EBESCT00000015537; EBESCP00000014828; EBESCG00000014597.

GeneID

948204.

GenomeReviews

Gene locus JW3674 in contig AP009048_GR.
Gene locus b3697 in contig U00096_GR.

KEGG

ecj:JW3674.
eco:b3697.

PATRIC

32122889. VBIEscCol129921_3820.

Organism-specific databases

EchoBASE

EB1181.

EcoGene

EG11195. yidA.

Phylogenomic databases

eggNOG

COG0561.

GeneTree

EBGT00050000009623.

HOGENOM

HBG728500.

OMA

YERYSIN.

PhylomeDB

P0A8Y5.

ProtClustDB

PRK10513.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11195-MONOMER.
MetaCyc:EG11195-MONOMER.

Gene expression databases

Genevestigator

P0A8Y5.

Family and domain databases

InterPro

IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
IPR006379. HAD-SF_hydro_IIB.
IPR000150. Hypothet_cof.
[Graphical view]

Gene3D

G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.

KO

K07024.

Pfam

PF00702. Hydrolase. 1 hit.
[Graphical view]

SUPFAM

SSF56784. HAD-like_dom. 1 hit.

TIGRFAMs

TIGR00099. Cof-subfamily. 1 hit.
TIGR01484. HAD-SF-IIB. 1 hit.

PROSITE

PS01228. COF_1. 1 hit.
PS01229. COF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

YIDA_ECOLI

Accession

Primary (citable) accession number: P0A8Y5
Secondary accession number(s): P09997, P76737, Q2M809

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program