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Protein

Fatty acid metabolism regulator protein

Gene

fadR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional regulator of fatty acid metabolism. Represses transcription of at least eight genes required for fatty acid transport and beta-oxidation including fadA, fadB, fadD, fadL and fadE. Activates transcription of at least three genes required for unsaturated fatty acid biosynthesis: fabA, fabB and iclR, the gene encoding the transcriptional regulator of the aceBAK operon encoding the glyoxylate shunt enzymes. Binding of FadR is specifically inhibited by long chain fatty acyl-CoA compounds.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi34 – 5320H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-HAMAP
  • fatty-acyl-CoA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • fatty acid oxidation Source: EcoCyc
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • positive regulation of fatty acid biosynthetic process Source: CACAO
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • regulation of fatty acid metabolic process Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD01520.
ECOL316407:JW1176-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid metabolism regulator proteinUniRule annotation
Gene namesi
Name:fadRUniRule annotation
Synonyms:oleR, thdB
Ordered Locus Names:b1187, JW1176
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10281. fadR.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 239238Fatty acid metabolism regulator proteinPRO_0000050626Add
BLAST

Proteomic databases

PaxDbiP0A8V6.
PRIDEiP0A8V6.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4260102. 14 interactions.
DIPiDIP-9564N.
IntActiP0A8V6. 2 interactions.
MINTiMINT-1264978.
STRINGi511145.b1187.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 2114Combined sources
Helixi34 – 418Combined sources
Helixi45 – 5713Combined sources
Beta strandi60 – 645Combined sources
Beta strandi67 – 715Combined sources
Helixi74 – 774Combined sources
Helixi80 – 823Combined sources
Helixi83 – 897Combined sources
Helixi91 – 933Combined sources
Helixi94 – 11926Combined sources
Helixi121 – 1299Combined sources
Turni130 – 1334Combined sources
Helixi138 – 15518Combined sources
Helixi159 – 17921Combined sources
Helixi183 – 20220Combined sources
Helixi206 – 22722Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E2XX-ray2.00A1-239[»]
1H9GX-ray2.10A1-239[»]
1H9TX-ray3.25A/B1-239[»]
1HW1X-ray1.50A/B1-239[»]
1HW2X-ray3.25A/B1-239[»]
ProteinModelPortaliP0A8V6.
SMRiP0A8V6. Positions 5-230.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8V6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 7469HTH gntR-typeUniRule annotationAdd
BLAST

Domaini

The C-terminal domain bind acyl-CoA.

Sequence similaritiesi

Contains 1 HTH gntR-type DNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C05. Bacteria.
COG2186. LUCA.
HOGENOMiHOG000278467.
InParanoidiP0A8V6.
KOiK03603.
OMAiIWETSGL.
PhylomeDBiP0A8V6.

Family and domain databases

CDDicd07377. WHTH_GntR. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
HAMAPiMF_00696. HTH_FadR. 1 hit.
InterProiIPR014178. FA-response_TF_FadR.
IPR028374. FadR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07840. FadR_C. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view]
PRINTSiPR00035. HTHGNTR.
SMARTiSM00345. HTH_GNTR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48008. SSF48008. 1 hit.
TIGRFAMsiTIGR02812. fadR_gamma. 1 hit.
PROSITEiPS50949. HTH_GNTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8V6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVIKAQSPAG FAEEYIIESI WNNRFPPGTI LPAERELSEL IGVTRTTLRE
60 70 80 90 100
VLQRLARDGW LTIQHGKPTK VNNFWETSGL NILETLARLD HESVPQLIDN
110 120 130 140 150
LLSVRTNIST IFIRTAFRQH PDKAQEVLAT ANEVADHADA FAELDYNIFR
160 170 180 190 200
GLAFASGNPI YGLILNGMKG LYTRIGRHYF ANPEARSLAL GFYHKLSALC
210 220 230
SEGAHDQVYE TVRRYGHESG EIWHRMQKNL PGDLAIQGR
Length:239
Mass (Da):26,969
Last modified:January 23, 2007 - v2
Checksum:i1FECA0DDAD5EB830
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08087 Genomic DNA. Translation: CAA30881.1.
U00096 Genomic DNA. Translation: AAC74271.1.
AP009048 Genomic DNA. Translation: BAA36042.1.
PIRiH64864.
RefSeqiNP_415705.1. NC_000913.3.
WP_000234823.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74271; AAC74271; b1187.
BAA36042; BAA36042; BAA36042.
GeneIDi948652.
KEGGiecj:JW1176.
eco:b1187.
PATRICi32117620. VBIEscCol129921_1232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X08087 Genomic DNA. Translation: CAA30881.1.
U00096 Genomic DNA. Translation: AAC74271.1.
AP009048 Genomic DNA. Translation: BAA36042.1.
PIRiH64864.
RefSeqiNP_415705.1. NC_000913.3.
WP_000234823.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E2XX-ray2.00A1-239[»]
1H9GX-ray2.10A1-239[»]
1H9TX-ray3.25A/B1-239[»]
1HW1X-ray1.50A/B1-239[»]
1HW2X-ray3.25A/B1-239[»]
ProteinModelPortaliP0A8V6.
SMRiP0A8V6. Positions 5-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260102. 14 interactions.
DIPiDIP-9564N.
IntActiP0A8V6. 2 interactions.
MINTiMINT-1264978.
STRINGi511145.b1187.

Proteomic databases

PaxDbiP0A8V6.
PRIDEiP0A8V6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74271; AAC74271; b1187.
BAA36042; BAA36042; BAA36042.
GeneIDi948652.
KEGGiecj:JW1176.
eco:b1187.
PATRICi32117620. VBIEscCol129921_1232.

Organism-specific databases

EchoBASEiEB0277.
EcoGeneiEG10281. fadR.

Phylogenomic databases

eggNOGiENOG4105C05. Bacteria.
COG2186. LUCA.
HOGENOMiHOG000278467.
InParanoidiP0A8V6.
KOiK03603.
OMAiIWETSGL.
PhylomeDBiP0A8V6.

Enzyme and pathway databases

BioCyciEcoCyc:PD01520.
ECOL316407:JW1176-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8V6.
PROiP0A8V6.

Family and domain databases

CDDicd07377. WHTH_GntR. 1 hit.
Gene3Di1.10.10.10. 1 hit.
1.20.120.530. 1 hit.
HAMAPiMF_00696. HTH_FadR. 1 hit.
InterProiIPR014178. FA-response_TF_FadR.
IPR028374. FadR_C.
IPR008920. TF_FadR/GntR_C.
IPR000524. Tscrpt_reg_HTH_GntR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF07840. FadR_C. 1 hit.
PF00392. GntR. 1 hit.
[Graphical view]
PRINTSiPR00035. HTHGNTR.
SMARTiSM00345. HTH_GNTR. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF48008. SSF48008. 1 hit.
TIGRFAMsiTIGR02812. fadR_gamma. 1 hit.
PROSITEiPS50949. HTH_GNTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADR_ECOLI
AccessioniPrimary (citable) accession number: P0A8V6
Secondary accession number(s): P09371, P76827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.