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Protein

DNA-directed RNA polymerase subunit beta

Gene

rpoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciEcoCyc:RPOB-MONOMER.
ECOL316407:JW3950-MONOMER.
MetaCyc:RPOB-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit beta (EC:2.7.7.6)
Short name:
RNAP subunit beta
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene namesi
Name:rpoB
Synonyms:groN, nitB, rif, ron, stl, stv, tabD
Ordered Locus Names:b3987, JW3950
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10894. rpoB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
  • DNA-directed RNA polymerase complex Source: GO_Central
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi813E → K: Disrupts the enzyme's active center. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1852.
DrugBankiDB00615. Rifabutin.
DB01045. Rifampicin.
DB01220. Rifaximin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000478921 – 1342DNA-directed RNA polymerase subunit betaAdd BLAST1342

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1022N6-acetyllysine1 Publication1
Modified residuei1200N6-acetyllysine1 Publication1

Post-translational modificationi

Acetylated on several lysine residues in the presence of glucose.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A8V2.
PaxDbiP0A8V2.
PRIDEiP0A8V2.

2D gel databases

SWISS-2DPAGEP0A8V2.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rapAP602403EBI-544996,EBI-551542
rpoCP0A8T77EBI-544996,EBI-543604

Protein-protein interaction databases

BioGridi4263472. 4 interactors.
852782. 1 interactor.
DIPiDIP-35777N.
IntActiP0A8V2. 99 interactors.
MINTiMINT-1222430.
STRINGi511145.b3987.

Chemistry databases

BindingDBiP0A8V2.

Structurei

Secondary structure

11342
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi154 – 159Combined sources6
Beta strandi172 – 177Combined sources6
Beta strandi179 – 181Combined sources3
Beta strandi184 – 188Combined sources5
Beta strandi194 – 198Combined sources5
Helixi207 – 212Combined sources6
Helixi217 – 224Combined sources8
Beta strandi227 – 233Combined sources7
Beta strandi236 – 240Combined sources5
Helixi243 – 246Combined sources4
Beta strandi255 – 257Combined sources3
Beta strandi260 – 263Combined sources4
Helixi271 – 279Combined sources9
Beta strandi284 – 286Combined sources3
Helixi289 – 293Combined sources5
Beta strandi295 – 297Combined sources3
Turni304 – 306Combined sources3
Beta strandi309 – 311Combined sources3
Helixi319 – 327Combined sources9
Beta strandi332 – 336Combined sources5
Beta strandi340 – 343Combined sources4
Helixi346 – 353Combined sources8
Helixi359 – 370Combined sources12
Helixi378 – 389Combined sources12
Turni392 – 394Combined sources3
Helixi398 – 408Combined sources11
Helixi422 – 437Combined sources16
Beta strandi832 – 843Combined sources12
Beta strandi849 – 852Combined sources4
Helixi859 – 863Combined sources5
Beta strandi869 – 871Combined sources3
Beta strandi882 – 884Combined sources3
Beta strandi886 – 889Combined sources4
Helixi892 – 894Combined sources3
Helixi897 – 906Combined sources10
Beta strandi913 – 915Combined sources3
Beta strandi925 – 936Combined sources12
Helixi943 – 980Combined sources38
Helixi986 – 991Combined sources6
Helixi994 – 996Combined sources3
Turni997 – 999Combined sources3
Beta strandi1003 – 1005Combined sources3
Helixi1006 – 1037Combined sources32
Beta strandi1046 – 1056Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IYDelectron microscopy-C1-1342[»]
3LTIX-ray1.60A152-443[»]
3LU0electron microscopy-C1-1342[»]
3T72X-ray4.33o/q896-910[»]
3TBIX-ray3.00B831-1057[»]
4JK1X-ray3.90C/H1-1342[»]
4JK2X-ray4.20C/H1-1342[»]
4KMUX-ray3.85C/H1-1342[»]
4KN4X-ray3.96C/H1-1342[»]
4KN7X-ray3.69C/H1-1342[»]
4MEXX-ray3.90C/I1-1342[»]
4MEYX-ray3.95C/I1-1342[»]
4S20X-ray4.70C/H1-1342[»]
4XSXX-ray3.71C/I1-1342[»]
4XSYX-ray4.01C/I1-1342[»]
4XSZX-ray3.68C/I1-1342[»]
4YG2X-ray3.70C/I1-1342[»]
4YLNX-ray5.50C/I/O1-1342[»]
4YLOX-ray6.00C/I/O1-1342[»]
4YLPX-ray5.50C/I/O1-1342[»]
4ZH2X-ray4.20C/I1-1342[»]
4ZH3X-ray4.08C/I1-1342[»]
4ZH4X-ray3.99C/I1-1342[»]
5BYHX-ray3.76C1-1342[»]
5EZKX-ray8.50C1-1342[»]
5IPLX-ray3.60C1-1342[»]
5IPMX-ray4.20C1-1342[»]
5IPNX-ray4.61C1-1342[»]
ProteinModelPortaliP0A8V2.
SMRiP0A8V2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8V2.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Phylogenomic databases

eggNOGiENOG4107QIH. Bacteria.
COG0085. LUCA.
HOGENOMiHOG000218612.
InParanoidiP0A8V2.
KOiK03043.
OMAiYFNPKRY.
PhylomeDBiP0A8V2.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
3.90.1110.10. 2 hits.
HAMAPiMF_01321. RNApol_bact_RpoB. 1 hit.
InterProiIPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR010243. RNA_pol_bsu_bac.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 5 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYSYTEKKR IRKDFGKRPQ VLDVPYLLSI QLDSFQKFIE QDPEGQYGLE
60 70 80 90 100
AAFRSVFPIQ SYSGNSELQY VSYRLGEPVF DVQECQIRGV TYSAPLRVKL
110 120 130 140 150
RLVIYEREAP EGTVKDIKEQ EVYMGEIPLM TDNGTFVING TERVIVSQLH
160 170 180 190 200
RSPGVFFDSD KGKTHSSGKV LYNARIIPYR GSWLDFEFDP KDNLFVRIDR
210 220 230 240 250
RRKLPATIIL RALNYTTEQI LDLFFEKVIF EIRDNKLQME LVPERLRGET
260 270 280 290 300
ASFDIEANGK VYVEKGRRIT ARHIRQLEKD DVKLIEVPVE YIAGKVVAKD
310 320 330 340 350
YIDESTGELI CAANMELSLD LLAKLSQSGH KRIETLFTND LDHGPYISET
360 370 380 390 400
LRVDPTNDRL SALVEIYRMM RPGEPPTREA AESLFENLFF SEDRYDLSAV
410 420 430 440 450
GRMKFNRSLL REEIEGSGIL SKDDIIDVMK KLIDIRNGKG EVDDIDHLGN
460 470 480 490 500
RRIRSVGEMA ENQFRVGLVR VERAVKERLS LGDLDTLMPQ DMINAKPISA
510 520 530 540 550
AVKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGPGGLTR ERAGFEVRDV
560 570 580 590 600
HPTHYGRVCP IETPEGPNIG LINSLSVYAQ TNEYGFLETP YRKVTDGVVT
610 620 630 640 650
DEIHYLSAIE EGNYVIAQAN SNLDEEGHFV EDLVTCRSKG ESSLFSRDQV
660 670 680 690 700
DYMDVSTQQV VSVGASLIPF LEHDDANRAL MGANMQRQAV PTLRADKPLV
710 720 730 740 750
GTGMERAVAV DSGVTAVAKR GGVVQYVDAS RIVIKVNEDE MYPGEAGIDI
760 770 780 790 800
YNLTKYTRSN QNTCINQMPC VSLGEPVERG DVLADGPSTD LGELALGQNM
810 820 830 840 850
RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELACVS RDTKLGPEEI
860 870 880 890 900
TADIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK
910 920 930 940 950
LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQVFTRDGVE KDKRALEIEE
960 970 980 990 1000
MQLKQAKKDL SEELQILEAG LFSRIRAVLV AGGVEAEKLD KLPRDRWLEL
1010 1020 1030 1040 1050
GLTDEEKQNQ LEQLAEQYDE LKHEFEKKLE AKRRKITQGD DLAPGVLKIV
1060 1070 1080 1090 1100
KVYLAVKRRI QPGDKMAGRH GNKGVISKIN PIEDMPYDEN GTPVDIVLNP
1110 1120 1130 1140 1150
LGVPSRMNIG QILETHLGMA AKGIGDKINA MLKQQQEVAK LREFIQRAYD
1160 1170 1180 1190 1200
LGADVRQKVD LSTFSDEEVM RLAENLRKGM PIATPVFDGA KEAEIKELLK
1210 1220 1230 1240 1250
LGDLPTSGQI RLYDGRTGEQ FERPVTVGYM YMLKLNHLVD DKMHARSTGS
1260 1270 1280 1290 1300
YSLVTQQPLG GKAQFGGQRF GEMEVWALEA YGAAYTLQEM LTVKSDDVNG
1310 1320 1330 1340
RTKMYKNIVD GNHQMEPGMP ESFNVLLKEI RSLGINIELE DE
Length:1,342
Mass (Da):150,632
Last modified:June 7, 2005 - v1
Checksum:iF9E95344C54AB118
GO

Sequence cautioni

The sequence described in Ref. 7 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → R AA sequence (PubMed:1095419).Curated1
Sequence conflicti106 – 107ER → G in CAA23629 (PubMed:7011900).Curated2
Sequence conflicti384 – 391LFENLFFS → CSRTCSSPT in CAA23629 (PubMed:7011900).Curated8
Sequence conflicti516D → V in CAA23625 (PubMed:6266829).Curated1
Sequence conflicti516D → V in CAA23627 (PubMed:6266829).Curated1
Sequence conflicti516D → V in AAA24585 (Ref. 11) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00339 Genomic DNA. Translation: CAA23625.1.
V00340 Genomic DNA. Translation: CAA23627.1.
U76222 Genomic DNA. Translation: AAB18647.1.
U00006 Genomic DNA. Translation: AAC43085.1.
U00096 Genomic DNA. Translation: AAC76961.1.
AP009048 Genomic DNA. Translation: BAE77333.1.
V00341 Genomic DNA. Translation: CAA23629.1.
M38292 Genomic DNA. Translation: AAA24579.1.
M38293 Genomic DNA. Translation: AAA24581.1.
M38287 Genomic DNA. Translation: AAA24585.1.
M38304 Genomic DNA. Translation: AAA24580.1.
U77436 Genomic DNA. Translation: AAD09605.1.
M38303 Genomic DNA. Translation: AAA24583.1.
PIRiF65205. RNECB.
RefSeqiNP_418414.1. NC_000913.3.
WP_000263098.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76961; AAC76961; b3987.
BAE77333; BAE77333; BAE77333.
GeneIDi948488.
KEGGiecj:JW3950.
eco:b3987.
PATRICi32123495. VBIEscCol129921_4100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00339 Genomic DNA. Translation: CAA23625.1.
V00340 Genomic DNA. Translation: CAA23627.1.
U76222 Genomic DNA. Translation: AAB18647.1.
U00006 Genomic DNA. Translation: AAC43085.1.
U00096 Genomic DNA. Translation: AAC76961.1.
AP009048 Genomic DNA. Translation: BAE77333.1.
V00341 Genomic DNA. Translation: CAA23629.1.
M38292 Genomic DNA. Translation: AAA24579.1.
M38293 Genomic DNA. Translation: AAA24581.1.
M38287 Genomic DNA. Translation: AAA24585.1.
M38304 Genomic DNA. Translation: AAA24580.1.
U77436 Genomic DNA. Translation: AAD09605.1.
M38303 Genomic DNA. Translation: AAA24583.1.
PIRiF65205. RNECB.
RefSeqiNP_418414.1. NC_000913.3.
WP_000263098.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IYDelectron microscopy-C1-1342[»]
3LTIX-ray1.60A152-443[»]
3LU0electron microscopy-C1-1342[»]
3T72X-ray4.33o/q896-910[»]
3TBIX-ray3.00B831-1057[»]
4JK1X-ray3.90C/H1-1342[»]
4JK2X-ray4.20C/H1-1342[»]
4KMUX-ray3.85C/H1-1342[»]
4KN4X-ray3.96C/H1-1342[»]
4KN7X-ray3.69C/H1-1342[»]
4MEXX-ray3.90C/I1-1342[»]
4MEYX-ray3.95C/I1-1342[»]
4S20X-ray4.70C/H1-1342[»]
4XSXX-ray3.71C/I1-1342[»]
4XSYX-ray4.01C/I1-1342[»]
4XSZX-ray3.68C/I1-1342[»]
4YG2X-ray3.70C/I1-1342[»]
4YLNX-ray5.50C/I/O1-1342[»]
4YLOX-ray6.00C/I/O1-1342[»]
4YLPX-ray5.50C/I/O1-1342[»]
4ZH2X-ray4.20C/I1-1342[»]
4ZH3X-ray4.08C/I1-1342[»]
4ZH4X-ray3.99C/I1-1342[»]
5BYHX-ray3.76C1-1342[»]
5EZKX-ray8.50C1-1342[»]
5IPLX-ray3.60C1-1342[»]
5IPMX-ray4.20C1-1342[»]
5IPNX-ray4.61C1-1342[»]
ProteinModelPortaliP0A8V2.
SMRiP0A8V2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263472. 4 interactors.
852782. 1 interactor.
DIPiDIP-35777N.
IntActiP0A8V2. 99 interactors.
MINTiMINT-1222430.
STRINGi511145.b3987.

Chemistry databases

BindingDBiP0A8V2.
ChEMBLiCHEMBL1852.
DrugBankiDB00615. Rifabutin.
DB01045. Rifampicin.
DB01220. Rifaximin.

2D gel databases

SWISS-2DPAGEP0A8V2.

Proteomic databases

EPDiP0A8V2.
PaxDbiP0A8V2.
PRIDEiP0A8V2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76961; AAC76961; b3987.
BAE77333; BAE77333; BAE77333.
GeneIDi948488.
KEGGiecj:JW3950.
eco:b3987.
PATRICi32123495. VBIEscCol129921_4100.

Organism-specific databases

EchoBASEiEB0887.
EcoGeneiEG10894. rpoB.

Phylogenomic databases

eggNOGiENOG4107QIH. Bacteria.
COG0085. LUCA.
HOGENOMiHOG000218612.
InParanoidiP0A8V2.
KOiK03043.
OMAiYFNPKRY.
PhylomeDBiP0A8V2.

Enzyme and pathway databases

BioCyciEcoCyc:RPOB-MONOMER.
ECOL316407:JW3950-MONOMER.
MetaCyc:RPOB-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8V2.
PROiP0A8V2.

Family and domain databases

CDDicd00653. RNA_pol_B_RPB2. 1 hit.
Gene3Di2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
3.90.1110.10. 2 hits.
HAMAPiMF_01321. RNApol_bact_RpoB. 1 hit.
InterProiIPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR010243. RNA_pol_bsu_bac.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERiPTHR20856. PTHR20856. 5 hits.
PfamiPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02013. rpoB. 1 hit.
PROSITEiPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOB_ECOLI
AccessioniPrimary (citable) accession number: P0A8V2
Secondary accession number(s): P00575
, P00576, P78242, Q2M8S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.