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Protein

Met repressor

Gene

metJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This regulatory protein, when combined with SAM (S-adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis. It is also autoregulated.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processAmino-acid biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD04032

Names & Taxonomyi

Protein namesi
Recommended name:
Met repressor
Alternative name(s):
Met regulon regulatory protein MetJ
Gene namesi
Name:metJ
Ordered Locus Names:b3938, JW3909
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10588 metJ

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001983972 – 105Met repressorAdd BLAST104

Proteomic databases

PaxDbiP0A8U6
PRIDEiP0A8U6

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
nusAP0AFF63EBI-555272,EBI-551571

Protein-protein interaction databases

BioGridi4263206, 7 interactors
DIPiDIP-47929N
IntActiP0A8U6, 1 interactor
STRINGi316385.ECDH10B_4127

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 15Combined sources5
Helixi18 – 21Combined sources4
Beta strandi22 – 30Combined sources9
Helixi31 – 47Combined sources17
Helixi54 – 67Combined sources14
Helixi74 – 77Combined sources4
Beta strandi79 – 81Combined sources3
Helixi87 – 95Combined sources9
Turni100 – 102Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMAX-ray2.80A/B2-105[»]
1CMBX-ray1.80A/B2-105[»]
1CMCX-ray1.80A/B2-105[»]
1MJ2X-ray2.40A/B/C/D2-105[»]
1MJKX-ray2.15A/B2-105[»]
1MJLX-ray2.10A/B2-105[»]
1MJMX-ray2.20A/B2-105[»]
1MJOX-ray2.10A/B/C/D2-105[»]
1MJPX-ray3.40A/B2-105[»]
1MJQX-ray2.40A/B/C/D/G/H/I/J2-105[»]
ProteinModelPortaliP0A8U6
SMRiP0A8U6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8U6

Family & Domainsi

Domaini

Does not bind DNA by a helix-turn-helix motif.

Sequence similaritiesi

Belongs to the MetJ family.Curated

Phylogenomic databases

eggNOGiCOG3060 LUCA
HOGENOMiHOG000271582
KOiK03764
OMAiKWNGEYI

Family and domain databases

CDDicd00490 Met_repressor_MetJ, 1 hit
Gene3Di1.10.140.10, 1 hit
HAMAPiMF_00744 MetJ, 1 hit
InterProiView protein in InterPro
IPR002084 Met_repressor_MetJ
IPR023453 Met_repressor_MetJ_dom_sf
IPR010985 Ribbon_hlx_hlx
PfamiView protein in Pfam
PF01340 MetJ, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD020365 Met_repressor_MetJ, 1 hit
SUPFAMiSSF47598 SSF47598, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEWSGEYIS PYAEHGKKSE QVKKITVSIP LKVLKILTDE RTRRQVNNLR
60 70 80 90 100
HATNSELLCE AFLHAFTGQP LPDDADLRKE RSDEIPEAAK EIMREMGINP

ETWEY
Length:105
Mass (Da):12,141
Last modified:January 23, 2007 - v2
Checksum:i05B0F54918C697EF
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti57L → Q in metJ193. 1
Natural varianti61A → T Hinders dimerization. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12869 Genomic DNA Translation: AAA24163.1
L19201 Genomic DNA Translation: AAB03070.1
U00096 Genomic DNA Translation: AAC76920.1
AP009048 Genomic DNA Translation: BAE77372.1
M38202 Genomic DNA Translation: AAA24162.1
PIRiA22660 RGECMJ
RefSeqiNP_418373.1, NC_000913.3
WP_000852812.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76920; AAC76920; b3938
BAE77372; BAE77372; BAE77372
GeneIDi948435
KEGGiecj:JW3909
eco:b3938
PATRICifig|1411691.4.peg.2767

Similar proteinsi

Entry informationi

Entry nameiMETJ_ECOLI
AccessioniPrimary (citable) accession number: P0A8U6
Secondary accession number(s): P08338, Q2M8N4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health