P0A8U6 (METJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Met repressor

Alternative name(s):
Met regulon regulatory protein metJ

Gene names

Name:	metJ
Ordered Locus Names:	b3938, JW3909

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This regulatory protein, when combined with SAM (S-adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis. It is also autoregulated. HAMAP MF_00744
Subunit structure	Homodimer.
Subcellular location	Cytoplasm HAMAP MF_00744.
Domain	Does not bind DNA by an helix-turn-helix motif. HAMAP MF_00744
Sequence similarities	Belongs to the metJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 105

104

Met repressor HAMAP MF_00744

PRO_0000198397

Natural variations

Natural variant

57

1

L → Q in metJ193.

Natural variant

61

1

A → T Hinders dimerization.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

105

Helix Strand Turn

Details...

Beta strand

11 – 15

5

Beta strand

22 – 30

9

Helix

31 – 47

17

Helix

54 – 67

14

Helix

74 – 77

4

Helix

87 – 95

9

Sequences

	Sequence		Length	Mass (Da)
	P0A8U6 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 05B0F54918C697EF	FASTA	105	12,141
	10 20 30 40 50 60 MAEWSGEYIS PYAEHGKKSE QVKKITVSIP LKVLKILTDE RTRRQVNNLR HATNSELLCE 70 80 90 100 AFLHAFTGQP LPDDADLRKE RSDEIPEAAK EIMREMGINP ETWEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and autoregulation of the metJ regulatory gene in Escherichia coli." Saint-Girons I., Duchange N., Cohen G.C., Zakin M.M. J. Biol. Chem. 259:14282-14285(1984) [PubMed: 6094549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression." Collier C.D., Johnson J.R. J. Bacteriol. 172:3918-3924(1990) [PubMed: 2141834] [Abstract] Cited for: VARIANT METJ193.
[6]	"Three-dimensional crystal structures of Escherichia coli met repressor with and without corepressor." Rafferty J.B., Somers W.S., Saint-Girons I., Phillips S.E.V. Nature 341:705-710(1989) [PubMed: 2677753] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[7]	"Crystal structure of the met repressor-operator complex at 2.8-A resolution reveals DNA recognition by beta-strands." Somers W.S., Phillips S.E.V. Nature 359:387-393(1992) [PubMed: 1406951] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
[8]	Garvie C.W., Phillips S.E.V. Submitted (JAN-1998) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M12869 Genomic DNA. Translation: AAA24163.1.
L19201 Genomic DNA. Translation: AAB03070.1.
U00096 Genomic DNA. Translation: AAC76920.1.
AP009048 Genomic DNA. Translation: BAE77372.1.
M38202 Genomic DNA. Translation: AAA24162.1.

PIR

RGECMJ. A22660.

RefSeq

NP_418373.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CMA	X-ray	2.80	A/B	2-105	[»]
1CMB	X-ray	1.80	A/B	2-105	[»]
1CMC	X-ray	1.80	A/B	2-105	[»]
1MJ2	X-ray	2.40	A/B/C/D	2-104	[»]
1MJK	X-ray	2.15	A/B	2-104	[»]
1MJL	X-ray	2.10	A/B	2-104	[»]
1MJM	X-ray	2.20	A/B	2-104	[»]
1MJO	X-ray	2.10	A/B/C/D	2-104	[»]
1MJP	X-ray	3.40	A/B	2-104	[»]
1MJQ	X-ray	2.40	A/B/C/D/G/H/I/J	2-104	[»]

ProteinModelPortal

P0A8U6.

SMR

P0A8U6. Positions 2-105.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47929N.

IntAct

P0A8U6. 1 interaction.

MINT

MINT-1265451.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002644; EBESCP00000002644; EBESCG00000002159.
EBESCT00000002645; EBESCP00000002645; EBESCG00000002159.
EBESCT00000002646; EBESCP00000002646; EBESCG00000002159.
EBESCT00000017229; EBESCP00000016520; EBESCG00000016288.

GeneID

948435.

GenomeReviews

Gene locus JW3909 in contig AP009048_GR.
Gene locus b3938 in contig U00096_GR.

KEGG

ecj:JW3909.
eco:b3938.

PATRIC

32123395. VBIEscCol129921_4058.

Organism-specific databases

EchoBASE

EB0583.

EcoGene

EG10588. metJ.

Phylogenomic databases

eggNOG

COG3060.

GeneTree

EBGT00050000009600.

HOGENOM

HBG297188.

OMA

DLRKERN.

ProtClustDB

PRK05264.

Enzyme and pathway databases

BioCyc

EcoCyc:PD04032.

Gene expression databases

Genevestigator

P0A8U6.

Family and domain databases

HAMAP

MF_00744. MetJ.
[Tree]

InterPro

IPR002084. Met_repressor_MetJ.
IPR023453. Met_repressor_MetJ_dom.
IPR010985. Ribbon_hlx_hlx.
[Graphical view]

Gene3D

G3DSA:1.10.140.10. MetJ. 1 hit.

KO

K03764.

Pfam

PF01340. MetJ. 1 hit.
[Graphical view]

ProDom

PD020365. Met_repressor_MetJ. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF47598. Met_repress_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

METJ_ECOLI

Accession

Primary (citable) accession number: P0A8U6
Secondary accession number(s): P08338, Q2M8N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program