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Protein

DNA-directed RNA polymerase subunit beta'

Gene

rpoC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

BioCyciEcoCyc:RPOC-MONOMER.
MetaCyc:RPOC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit beta'UniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit beta'UniRule annotation
Alternative name(s):
RNA polymerase subunit beta'UniRule annotation
Transcriptase subunit beta'UniRule annotation
Gene namesi
Name:rpoCUniRule annotation
Synonyms:tabB
Ordered Locus Names:b3988, JW3951
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10895. rpoC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
  • DNA-directed RNA polymerase complex Source: GO_Central
  • membrane Source: UniProtKB

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2364672.
DrugBankiDB00615. Rifabutin.
DB01045. Rifampicin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000677411 – 1407DNA-directed RNA polymerase subunit beta'Add BLAST1407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei983N6-acetyllysineUniRule annotation1 Publication1

Post-translational modificationi

Acetylated on several lysine residues in the presence of glucose.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP0A8T7.
PRIDEiP0A8T7.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4262959. 22 interactors.
DIPiDIP-35803N.
IntActiP0A8T7. 113 interactors.
MINTiMINT-1220667.
STRINGi316385.ECDH10B_4176.

Chemistry databases

BindingDBiP0A8T7.

Structurei

Secondary structure

11407
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi20 – 24Combined sources5
Helixi27 – 33Combined sources7
Beta strandi34 – 37Combined sources4
Beta strandi46 – 48Combined sources3
Beta strandi55 – 57Combined sources3
Turni59 – 62Combined sources4
Beta strandi71 – 74Combined sources4
Beta strandi86 – 88Combined sources3
Helixi95 – 99Combined sources5
Beta strandi102 – 112Combined sources11
Helixi114 – 118Combined sources5
Beta strandi119 – 121Combined sources3
Helixi123 – 127Combined sources5
Helixi132 – 139Combined sources8
Beta strandi142 – 148Combined sources7
Beta strandi151 – 153Combined sources3
Helixi162 – 171Combined sources10
Beta strandi177 – 179Combined sources3
Helixi182 – 191Combined sources10
Helixi194 – 207Combined sources14
Helixi211 – 230Combined sources20
Helixi234 – 237Combined sources4
Beta strandi238 – 244Combined sources7
Helixi247 – 249Combined sources3
Helixi256 – 258Combined sources3
Beta strandi260 – 262Combined sources3
Helixi265 – 284Combined sources20
Helixi289 – 307Combined sources19
Beta strandi311 – 313Combined sources3
Beta strandi321 – 323Combined sources3
Helixi327 – 331Combined sources5
Beta strandi334 – 336Combined sources3
Turni338 – 340Combined sources3
Beta strandi341 – 343Combined sources3
Beta strandi348 – 356Combined sources9
Beta strandi365 – 369Combined sources5
Helixi370 – 376Combined sources7
Helixi378 – 385Combined sources8
Turni386 – 389Combined sources4
Beta strandi390 – 393Combined sources4
Helixi394 – 403Combined sources10
Helixi408 – 416Combined sources9
Beta strandi421 – 424Combined sources4
Helixi431 – 433Combined sources3
Beta strandi434 – 445Combined sources12
Beta strandi447 – 449Combined sources3
Helixi451 – 455Combined sources5
Turni456 – 458Combined sources3
Beta strandi461 – 463Combined sources3
Beta strandi465 – 469Combined sources5
Helixi474 – 482Combined sources9
Turni486 – 488Combined sources3
Beta strandi493 – 499Combined sources7
Helixi505 – 513Combined sources9
Turni521 – 524Combined sources4
Beta strandi526 – 529Combined sources4
Helixi530 – 538Combined sources9
Beta strandi547 – 556Combined sources10
Turni559 – 561Combined sources3
Beta strandi564 – 573Combined sources10
Helixi574 – 579Combined sources6
Turni580 – 582Combined sources3
Helixi589 – 591Combined sources3
Helixi598 – 612Combined sources15
Helixi614 – 635Combined sources22
Helixi641 – 643Combined sources3
Helixi650 – 669Combined sources20
Helixi675 – 703Combined sources29
Beta strandi709 – 712Combined sources4
Helixi721 – 728Combined sources8
Helixi734 – 740Combined sources7
Beta strandi763 – 765Combined sources3
Helixi769 – 803Combined sources35
Beta strandi820 – 822Combined sources3
Beta strandi828 – 830Combined sources3
Helixi835 – 839Combined sources5
Beta strandi843 – 846Combined sources4
Beta strandi851 – 854Combined sources4
Beta strandi856 – 858Combined sources3
Helixi866 – 874Combined sources9
Beta strandi880 – 883Combined sources4
Turni885 – 887Combined sources3
Beta strandi891 – 894Combined sources4
Helixi896 – 899Combined sources4
Beta strandi903 – 907Combined sources5
Helixi915 – 924Combined sources10
Helixi925 – 929Combined sources5
Helixi945 – 948Combined sources4
Beta strandi949 – 951Combined sources3
Beta strandi956 – 962Combined sources7
Beta strandi965 – 967Combined sources3
Beta strandi973 – 975Combined sources3
Beta strandi981 – 985Combined sources5
Beta strandi991 – 996Combined sources6
Beta strandi1002 – 1005Combined sources4
Beta strandi1016 – 1019Combined sources4
Beta strandi1022 – 1028Combined sources7
Beta strandi1033 – 1039Combined sources7
Turni1043 – 1045Combined sources3
Beta strandi1046 – 1049Combined sources4
Turni1052 – 1054Combined sources3
Beta strandi1058 – 1061Combined sources4
Helixi1064 – 1066Combined sources3
Helixi1071 – 1073Combined sources3
Beta strandi1077 – 1081Combined sources5
Beta strandi1093 – 1096Combined sources4
Beta strandi1098 – 1100Combined sources3
Beta strandi1106 – 1109Combined sources4
Beta strandi1120 – 1125Combined sources6
Helixi1138 – 1145Combined sources8
Beta strandi1158 – 1165Combined sources8
Beta strandi1170 – 1178Combined sources9
Beta strandi1180 – 1182Combined sources3
Beta strandi1187 – 1190Combined sources4
Beta strandi1202 – 1204Combined sources3
Beta strandi1209 – 1211Combined sources3
Helixi1217 – 1222Combined sources6
Helixi1226 – 1243Combined sources18
Helixi1250 – 1260Combined sources11
Beta strandi1263 – 1266Combined sources4
Beta strandi1270 – 1272Combined sources3
Beta strandi1277 – 1279Combined sources3
Helixi1280 – 1291Combined sources12
Beta strandi1292 – 1294Combined sources3
Beta strandi1302 – 1304Combined sources3
Helixi1309 – 1312Combined sources4
Helixi1319 – 1325Combined sources7
Helixi1328 – 1338Combined sources11
Helixi1347 – 1353Combined sources7
Helixi1361 – 1372Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AUKX-ray2.30A/B/C/D/E944-1129[»]
2LMCNMR-B1151-1213[»]
3IYDelectron microscopy-D1-1407[»]
3LU0electron microscopy-D1-1407[»]
4IQZX-ray2.10A/B/C/D/E932-1141[»]
4JK1X-ray3.90D/I1-1407[»]
4JK2X-ray4.20D/I1-1407[»]
4KMUX-ray3.85D/I1-1407[»]
4KN4X-ray3.96D/I1-1407[»]
4KN7X-ray3.69D/I1-1407[»]
4MEXX-ray3.90D/J1-1407[»]
4MEYX-ray3.95D/J1-1407[»]
4XSXX-ray3.71D/J1-1407[»]
4XSYX-ray4.01D/J1-1407[»]
4XSZX-ray3.68D/J1-1407[»]
4YG2X-ray3.70D/J1-1407[»]
4YLNX-ray5.50D/J/P1-1407[»]
4YLOX-ray6.00D/J/P1-1407[»]
4YLPX-ray5.50D/J/P1-1407[»]
4ZH2X-ray4.20D/J1-1407[»]
4ZH3X-ray4.08D/J1-1407[»]
4ZH4X-ray3.99D/J1-1407[»]
5BYHX-ray3.76D1-1407[»]
5EZKX-ray8.50D1-1407[»]
5IPLX-ray3.60D1-1407[»]
5IPMX-ray4.20D1-1407[»]
5IPNX-ray4.61D1-1407[»]
5MS0electron microscopy9.80D1-1407[»]
5MY1electron microscopy7.60Y1-1407[»]
5UACX-ray3.80D/J1-1407[»]
5UAGX-ray3.40D/J1-1407[»]
5UAHX-ray4.10D/J1-1407[»]
5UAJX-ray3.92D/J1-1407[»]
5UALX-ray3.89D/J1-1407[»]
5UAQX-ray3.60D/J1-1407[»]
5UP6electron microscopy3.74J1-1407[»]
5UPCelectron microscopy4.38J1-1407[»]
5VSWX-ray4.29D/J1-1407[»]
5W1SX-ray3.81D/J1-1407[»]
5W1TX-ray4.50D/J1-1407[»]
ProteinModelPortaliP0A8T7.
SMRiP0A8T7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8T7.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D27. Bacteria.
COG0086. LUCA.
HOGENOMiHOG000218386.
InParanoidiP0A8T7.
KOiK03046.
PhylomeDBiP0A8T7.

Family and domain databases

HAMAPiMF_01322. RNApol_bact_RpoC. 1 hit.
InterProiView protein in InterPro
IPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
PfamiView protein in Pfam
PF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 2 hits.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
SMARTiView protein in SMART
SM00663. RPOLA_N. 1 hit.
TIGRFAMsiTIGR02386. rpoC_TIGR. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK
60 70 80 90 100
PERDGLFCAR IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQTKVRRE
110 120 130 140 150
RMGHIELASP TAHIWFLKSL PSRIGLLLDM PLRDIERVLY FESYVVIEGG
160 170 180 190 200
MTNLERQQIL TEEQYLDALE EFGDEFDAKM GAEAIQALLK SMDLEQECEQ
210 220 230 240 250
LREELNETNS ETKRKKLTKR IKLLEAFVQS GNKPEWMILT VLPVLPPDLR
260 270 280 290 300
PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ
310 320 330 340 350
EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS
360 370 380 390 400
GRSVITVGPY LRLHQCGLPK KMALELFKPF IYGKLELRGL ATTIKAAKKM
410 420 430 440 450
VEREEAVVWD ILDEVIREHP VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH
460 470 480 490 500
PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA RALMMSTNNI LSPANGEPII
510 520 530 540 550
VPSQDVVLGL YYMTRDCVNA KGEGMVLTGP KEAERLYRSG LASLHARVKV
560 570 580 590 600
RITEYEKDAN GELVAKTSLK DTTVGRAILW MIVPKGLPYS IVNQALGKKA
610 620 630 640 650
ISKMLNTCYR ILGLKPTVIF ADQIMYTGFA YAARSGASVG IDDMVIPEKK
660 670 680 690 700
HEIISEAEAE VAEIQEQFQS GLVTAGERYN KVIDIWAAAN DRVSKAMMDN
710 720 730 740 750
LQTETVINRD GQEEKQVSFN SIYMMADSGA RGSAAQIRQL AGMRGLMAKP
760 770 780 790 800
DGSIIETPIT ANFREGLNVL QYFISTHGAR KGLADTALKT ANSGYLTRRL
810 820 830 840 850
VDVAQDLVVT EDDCGTHEGI MMTPVIEGGD VKEPLRDRVL GRVTAEDVLK
860 870 880 890 900
PGTADILVPR NTLLHEQWCD LLEENSVDAV KVRSVVSCDT DFGVCAHCYG
910 920 930 940 950
RDLARGHIIN KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAAAESSI
960 970 980 990 1000
QVKNKGSIKL SNVKSVVNSS GKLVITSRNT ELKLIDEFGR TKESYKVPYG
1010 1020 1030 1040 1050
AVLAKGDGEQ VAGGETVANW DPHTMPVITE VSGFVRFTDM IDGQTITRQT
1060 1070 1080 1090 1100
DELTGLSSLV VLDSAERTAG GKDLRPALKI VDAQGNDVLI PGTDMPAQYF
1110 1120 1130 1140 1150
LPGKAIVQLE DGVQISSGDT LARIPQESGG TKDITGGLPR VADLFEARRP
1160 1170 1180 1190 1200
KEPAILAEIS GIVSFGKETK GKRRLVITPV DGSDPYEEMI PKWRQLNVFE
1210 1220 1230 1240 1250
GERVERGDVI SDGPEAPHDI LRLRGVHAVT RYIVNEVQDV YRLQGVKIND
1260 1270 1280 1290 1300
KHIEVIVRQM LRKATIVNAG SSDFLEGEQV EYSRVKIANR ELEANGKVGA
1310 1320 1330 1340 1350
TYSRDLLGIT KASLATESFI SAASFQETTR VLTEAAVAGK RDELRGLKEN
1360 1370 1380 1390 1400
VIVGRLIPAG TGYAYHQDRM RRRAAGEAPA APQVTAEDAS ASLAELLNAG

LGGSDNE
Length:1,407
Mass (Da):155,160
Last modified:July 21, 1986 - v1
Checksum:iA6878C61D15F4961
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1133 – 1177DITGG…RRLVI → ASPVVCRALRTCSKHVVRKS RQSWLKSAVSFPSVKKPKVN VVWLS (PubMed:6278450).CuratedAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00339 Genomic DNA. Translation: CAA23626.1.
U00006 Genomic DNA. Translation: AAC43086.1.
U00096 Genomic DNA. Translation: AAC76962.1.
AP009048 Genomic DNA. Translation: BAE77332.1.
M38293 Genomic DNA. Translation: AAA24582.1.
M38303 Genomic DNA. Translation: AAA24584.1. Sequence problems.
V00340 Genomic DNA. Translation: CAA23628.1.
M38305 Genomic DNA. Translation: AAA24586.1.
M38288 Genomic DNA. Translation: AAA24408.1.
PIRiA00695. RNECC.
RefSeqiNP_418415.1. NC_000913.3.
WP_000653944.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76962; AAC76962; b3988.
BAE77332; BAE77332; BAE77332.
GeneIDi948487.
KEGGiecj:JW3951.
eco:b3988.
PATRICifig|1411691.4.peg.2724.

Similar proteinsi

Entry informationi

Entry nameiRPOC_ECOLI
AccessioniPrimary (citable) accession number: P0A8T7
Secondary accession number(s): P00577
, P00578, P78134, Q2M8S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: August 30, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families