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P0A8T7 (RPOC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta'

Short name=RNAP subunit beta'
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'
Gene names
Name:rpoC
Synonyms:tabB
Ordered Locus Names:b3988, JW3951
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length1407 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Ref.12

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity. Ref.12 Ref.15

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14071407DNA-directed RNA polymerase subunit beta' HAMAP-Rule MF_01322
PRO_0000067741

Amino acid modifications

Modified residue9831N6-acetyllysine Ref.13

Experimental info

Sequence conflict1133 – 117745DITGG…RRLVI → ASPVVCRALRTCSKHVVRKS RQSWLKSAVSFPSVKKPKVN VVWLS Ref.11

Secondary structure

....................................................... 1407
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A8T7 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: A6878C61D15F4961

FASTA1,407155,160
        10         20         30         40         50         60 
MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR 

        70         80         90        100        110        120 
IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQTKVRRE RMGHIELASP TAHIWFLKSL 

       130        140        150        160        170        180 
PSRIGLLLDM PLRDIERVLY FESYVVIEGG MTNLERQQIL TEEQYLDALE EFGDEFDAKM 

       190        200        210        220        230        240 
GAEAIQALLK SMDLEQECEQ LREELNETNS ETKRKKLTKR IKLLEAFVQS GNKPEWMILT 

       250        260        270        280        290        300 
VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ 

       310        320        330        340        350        360 
EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPY 

       370        380        390        400        410        420 
LRLHQCGLPK KMALELFKPF IYGKLELRGL ATTIKAAKKM VEREEAVVWD ILDEVIREHP 

       430        440        450        460        470        480 
VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA 

       490        500        510        520        530        540 
RALMMSTNNI LSPANGEPII VPSQDVVLGL YYMTRDCVNA KGEGMVLTGP KEAERLYRSG 

       550        560        570        580        590        600 
LASLHARVKV RITEYEKDAN GELVAKTSLK DTTVGRAILW MIVPKGLPYS IVNQALGKKA 

       610        620        630        640        650        660 
ISKMLNTCYR ILGLKPTVIF ADQIMYTGFA YAARSGASVG IDDMVIPEKK HEIISEAEAE 

       670        680        690        700        710        720 
VAEIQEQFQS GLVTAGERYN KVIDIWAAAN DRVSKAMMDN LQTETVINRD GQEEKQVSFN 

       730        740        750        760        770        780 
SIYMMADSGA RGSAAQIRQL AGMRGLMAKP DGSIIETPIT ANFREGLNVL QYFISTHGAR 

       790        800        810        820        830        840 
KGLADTALKT ANSGYLTRRL VDVAQDLVVT EDDCGTHEGI MMTPVIEGGD VKEPLRDRVL 

       850        860        870        880        890        900 
GRVTAEDVLK PGTADILVPR NTLLHEQWCD LLEENSVDAV KVRSVVSCDT DFGVCAHCYG 

       910        920        930        940        950        960 
RDLARGHIIN KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAAAESSI QVKNKGSIKL 

       970        980        990       1000       1010       1020 
SNVKSVVNSS GKLVITSRNT ELKLIDEFGR TKESYKVPYG AVLAKGDGEQ VAGGETVANW 

      1030       1040       1050       1060       1070       1080 
DPHTMPVITE VSGFVRFTDM IDGQTITRQT DELTGLSSLV VLDSAERTAG GKDLRPALKI 

      1090       1100       1110       1120       1130       1140 
VDAQGNDVLI PGTDMPAQYF LPGKAIVQLE DGVQISSGDT LARIPQESGG TKDITGGLPR 

      1150       1160       1170       1180       1190       1200 
VADLFEARRP KEPAILAEIS GIVSFGKETK GKRRLVITPV DGSDPYEEMI PKWRQLNVFE 

      1210       1220       1230       1240       1250       1260 
GERVERGDVI SDGPEAPHDI LRLRGVHAVT RYIVNEVQDV YRLQGVKIND KHIEVIVRQM 

      1270       1280       1290       1300       1310       1320 
LRKATIVNAG SSDFLEGEQV EYSRVKIANR ELEANGKVGA TYSRDLLGIT KASLATESFI 

      1330       1340       1350       1360       1370       1380 
SAASFQETTR VLTEAAVAGK RDELRGLKEN VIVGRLIPAG TGYAYHQDRM RRRAAGEAPA 

      1390       1400 
APQVTAEDAS ASLAELLNAG LGGSDNE 

« Hide

References

« Hide 'large scale' references
[1]"The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit."
Ovchinnikov Y.A., Monastyrskaya G.S., Gubanov V.V., Guryev S.O., Salomatina I.S., Shuvaeva T.M., Lipkin V.M., Sverdlov E.D.
Nucleic Acids Res. 10:4035-4044(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, PARTIAL PROTEIN SEQUENCE.
[2]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Primary structure of EcoRI-F fragment of rpoB, C genes and corresponding fragments of beta- and beta'-subunits of RNA polymerase from E.coli."
Monastyrskaya G.S., Gubanov V.V., Guryev S.O., Lipkin V.M., Sverdlov E.D.
Bioorg. Khim. 6:1106-1109(1980)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176.
[6]"Structure of a central part of E.coli operon rpoBC. Nucleotide sequence of the gene for beta subunit of RNA polymerase."
Gurevich A.I., Igoshin A.V., Kolosov M.N.
Bioorg. Khim. 6:1580-1584(1980)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176.
[7]"The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit."
Ovchinnikov Y.A., Monastyrskaya G.S., Gubanov V.V., Guryev S.O., Chertov O.Y., Modyanov N.N., Grinkevich V.A., Makarova I.A., Marchenko T.V., Polovnikova I.N., Lipkin V.M., Sverdlov E.D.
Eur. J. Biochem. 116:621-629(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176, PROTEIN SEQUENCE OF 1-8.
[8]"The subunits of DNA-dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N-terminal regions."
Fujiki H., Zurek G.
FEBS Lett. 55:242-244(1975) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-4.
Strain: K12.
[9]"Primary structure of EcoRI-D fragment of rpoC gene and corresponding fragment of beta-subunit of RNA polymerase from E.coli."
Monastyrskaya G.S., Guryev S.O., Kalinina N.F., Sorokin A.V., Salomatina I.S., Shuvaeva T.M., Lipkin V.M., Sverdlov E.D., Ovchinnikov Y.A.
Bioorg. Khim. 8:130-134(1982)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 175-988.
[10]"Primary structure of RNA polymerase from E.coli. Nucleotide sequence of E.coli DNA fragment containing a part of the rpoC gene and the corresponding C-terminal amino acid sequence of beta'-subunit."
Ovchinnikov Y.A., Monastyrskaya G.S., Gubanov V.V., Salomatina I.S., Shuvaeva T.M., Lipkin V.M., Sverdlov E.D.
Bioorg. Khim. 7:1107-1112(1981)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 987-1407.
[11]"Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC)."
Squires C., Krainer A., Barry G., Shen W.-F., Squires C.L.
Nucleic Acids Res. 9:6827-6840(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1073-1407.
Strain: K12.
[12]"Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP."
Igarashi K., Ishihama A.
Cell 65:1015-1022(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN TRANSCRIPTION, SUBUNIT.
[13]"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-983, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
[14]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[15]"Three-dimensional EM structure of an intact activator-dependent transcription initiation complex."
Hudson B.P., Quispe J., Lara-Gonzalez S., Kim Y., Berman H.M., Arnold E., Ebright R.H., Lawson C.L.
Proc. Natl. Acad. Sci. U.S.A. 106:19830-19835(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (19.80 ANGSTROMS) IN COMPLEX WITH RPOA; RPOB; RPOD; RPOZ; CRP AND DNA, DNA-BINDING, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00339 Genomic DNA. Translation: CAA23626.1.
U00006 Genomic DNA. Translation: AAC43086.1.
U00096 Genomic DNA. Translation: AAC76962.1.
AP009048 Genomic DNA. Translation: BAE77332.1.
M38293 Genomic DNA. Translation: AAA24582.1.
M38303 Genomic DNA. Translation: AAA24584.1. Sequence problems.
V00340 Genomic DNA. Translation: CAA23628.1.
M38305 Genomic DNA. Translation: AAA24586.1.
M38288 Genomic DNA. Translation: AAA24408.1.
PIRRNECC. A00695.
RefSeqNP_418415.1. NC_000913.3.
YP_491473.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2AUKX-ray2.30A/B/C/D/E944-1129[»]
2LMCNMR-B1151-1213[»]
3IYDelectron microscopy-D1-1407[»]
3LU0electron microscopy-D1-1407[»]
4IGCX-ray3.70D/I1-1407[»]
4IQZX-ray2.10A/B/C/D/E932-1141[»]
4JK1X-ray3.90D/I1-1407[»]
4JK2X-ray4.20D/I1-1407[»]
4KMUX-ray3.85D/I1-1407[»]
4KN4X-ray3.96D/I1-1407[»]
4KN7X-ray3.69D/I1-1407[»]
4MEXX-ray3.90D/J1-1407[»]
4MEYX-ray3.95D/J1-1407[»]
ProteinModelPortalP0A8T7.
SMRP0A8T7. Positions 15-1382.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35803N.
IntActP0A8T7. 113 interactions.
MINTMINT-1220667.
STRING511145.b3988.

Chemistry

ChEMBLCHEMBL2364672.

PTM databases

PhosSiteP010445.

Proteomic databases

PaxDbP0A8T7.
PRIDEP0A8T7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76962; AAC76962; b3988.
BAE77332; BAE77332; BAE77332.
GeneID12933965.
948487.
KEGGecj:Y75_p3209.
eco:b3988.
PATRIC32123497. VBIEscCol129921_4101.

Organism-specific databases

EchoBASEEB0888.
EcoGeneEG10895. rpoC.

Phylogenomic databases

eggNOGCOG0086.
HOGENOMHOG000218386.
KOK03046.
OMAYDLVNQN.
OrthoDBEOG6M9DS6.
PhylomeDBP0A8T7.

Enzyme and pathway databases

BioCycEcoCyc:RPOC-MONOMER.
ECOL316407:JW3951-MONOMER.
MetaCyc:RPOC-MONOMER.

Gene expression databases

GenevestigatorP0A8T7.

Family and domain databases

HAMAPMF_01322. RNApol_bact_RpoC.
MF_01323. RNApol_bact_RpoC1.
InterProIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTSM00663. RPOLA_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR02386. rpoC_TIGR. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A8T7.
PROP0A8T7.

Entry information

Entry nameRPOC_ECOLI
AccessionPrimary (citable) accession number: P0A8T7
Secondary accession number(s): P00577 expand/collapse secondary AC list , P00578, P78134, Q2M8S4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene