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Protein

DNA-directed RNA polymerase subunit beta'

Gene

rpoC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciEcoCyc:RPOC-MONOMER.
ECOL316407:JW3951-MONOMER.
MetaCyc:RPOC-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit beta'UniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit beta'UniRule annotation
Alternative name(s):
RNA polymerase subunit beta'UniRule annotation
Transcriptase subunit beta'UniRule annotation
Gene namesi
Name:rpoCUniRule annotation
Synonyms:tabB
Ordered Locus Names:b3988, JW3951
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10895. rpoC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: EcoCyc
  • DNA-directed RNA polymerase complex Source: GO_Central
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2364672.
DrugBankiDB00615. Rifabutin.
DB01045. Rifampicin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14071407DNA-directed RNA polymerase subunit beta'PRO_0000067741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei983 – 9831N6-acetyllysineUniRule annotation1 Publication

Post-translational modificationi

Acetylated on several lysine residues in the presence of glucose.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A8T7.
PaxDbiP0A8T7.
PRIDEiP0A8T7.

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
htpGP0A6Z32EBI-543604,EBI-369221
rpoBP0A8V27EBI-543604,EBI-544996
rpoDP005798EBI-543604,EBI-545104

Protein-protein interaction databases

BioGridi4262959. 22 interactions.
DIPiDIP-35803N.
IntActiP0A8T7. 113 interactions.
MINTiMINT-1220667.
STRINGi511145.b3988.

Chemistry

BindingDBiP0A8T7.

Structurei

Secondary structure

1
1407
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi945 – 9484Combined sources
Beta strandi949 – 9513Combined sources
Beta strandi956 – 9627Combined sources
Beta strandi965 – 9673Combined sources
Beta strandi973 – 9753Combined sources
Beta strandi981 – 9855Combined sources
Beta strandi991 – 9966Combined sources
Beta strandi1002 – 10054Combined sources
Beta strandi1016 – 10194Combined sources
Beta strandi1022 – 10287Combined sources
Beta strandi1033 – 10397Combined sources
Turni1043 – 10453Combined sources
Beta strandi1046 – 10494Combined sources
Turni1052 – 10543Combined sources
Beta strandi1058 – 10614Combined sources
Helixi1064 – 10663Combined sources
Helixi1071 – 10733Combined sources
Beta strandi1077 – 10815Combined sources
Beta strandi1093 – 10964Combined sources
Beta strandi1098 – 11003Combined sources
Beta strandi1106 – 11094Combined sources
Beta strandi1120 – 11256Combined sources
Beta strandi1155 – 116612Combined sources
Beta strandi1169 – 11713Combined sources
Beta strandi1174 – 118310Combined sources
Beta strandi1186 – 11894Combined sources
Beta strandi1202 – 12043Combined sources
Beta strandi1209 – 12113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AUKX-ray2.30A/B/C/D/E944-1129[»]
2LMCNMR-B1151-1213[»]
3IYDelectron microscopy-D1-1407[»]
3LU0electron microscopy-D1-1407[»]
4IQZX-ray2.10A/B/C/D/E932-1141[»]
4JK1X-ray3.90D/I1-1407[»]
4JK2X-ray4.20D/I1-1407[»]
4KMUX-ray3.85D/I1-1407[»]
4KN4X-ray3.96D/I1-1407[»]
4KN7X-ray3.69D/I1-1407[»]
4MEXX-ray3.90D/J1-1407[»]
4MEYX-ray3.95D/J1-1407[»]
4XSXX-ray3.71D/J1-1407[»]
4XSYX-ray4.01D/J1-1407[»]
4XSZX-ray3.68D/J1-1407[»]
4YG2X-ray3.70D/J1-1407[»]
4YLNX-ray5.50D/J/P1-1407[»]
4YLOX-ray6.00D/J/P1-1407[»]
4YLPX-ray5.50D/J/P1-1407[»]
4ZH2X-ray4.20D/J1-1407[»]
4ZH3X-ray4.08D/J1-1407[»]
4ZH4X-ray3.99D/J1-1407[»]
5BYHX-ray3.76D1-1407[»]
5EZKX-ray8.50D1-1407[»]
5IPLX-ray3.60D1-1407[»]
5IPMX-ray4.20D1-1407[»]
5IPNX-ray4.61D1-1407[»]
ProteinModelPortaliP0A8T7.
SMRiP0A8T7. Positions 15-1376.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8T7.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA polymerase beta' chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D27. Bacteria.
COG0086. LUCA.
HOGENOMiHOG000218386.
InParanoidiP0A8T7.
KOiK03046.
OMAiYDLVNQN.
PhylomeDBiP0A8T7.

Family and domain databases

HAMAPiMF_01322. RNApol_bact_RpoC. 1 hit.
InterProiIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 2 hits.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02386. rpoC_TIGR. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK
60 70 80 90 100
PERDGLFCAR IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQTKVRRE
110 120 130 140 150
RMGHIELASP TAHIWFLKSL PSRIGLLLDM PLRDIERVLY FESYVVIEGG
160 170 180 190 200
MTNLERQQIL TEEQYLDALE EFGDEFDAKM GAEAIQALLK SMDLEQECEQ
210 220 230 240 250
LREELNETNS ETKRKKLTKR IKLLEAFVQS GNKPEWMILT VLPVLPPDLR
260 270 280 290 300
PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ
310 320 330 340 350
EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS
360 370 380 390 400
GRSVITVGPY LRLHQCGLPK KMALELFKPF IYGKLELRGL ATTIKAAKKM
410 420 430 440 450
VEREEAVVWD ILDEVIREHP VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH
460 470 480 490 500
PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA RALMMSTNNI LSPANGEPII
510 520 530 540 550
VPSQDVVLGL YYMTRDCVNA KGEGMVLTGP KEAERLYRSG LASLHARVKV
560 570 580 590 600
RITEYEKDAN GELVAKTSLK DTTVGRAILW MIVPKGLPYS IVNQALGKKA
610 620 630 640 650
ISKMLNTCYR ILGLKPTVIF ADQIMYTGFA YAARSGASVG IDDMVIPEKK
660 670 680 690 700
HEIISEAEAE VAEIQEQFQS GLVTAGERYN KVIDIWAAAN DRVSKAMMDN
710 720 730 740 750
LQTETVINRD GQEEKQVSFN SIYMMADSGA RGSAAQIRQL AGMRGLMAKP
760 770 780 790 800
DGSIIETPIT ANFREGLNVL QYFISTHGAR KGLADTALKT ANSGYLTRRL
810 820 830 840 850
VDVAQDLVVT EDDCGTHEGI MMTPVIEGGD VKEPLRDRVL GRVTAEDVLK
860 870 880 890 900
PGTADILVPR NTLLHEQWCD LLEENSVDAV KVRSVVSCDT DFGVCAHCYG
910 920 930 940 950
RDLARGHIIN KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAAAESSI
960 970 980 990 1000
QVKNKGSIKL SNVKSVVNSS GKLVITSRNT ELKLIDEFGR TKESYKVPYG
1010 1020 1030 1040 1050
AVLAKGDGEQ VAGGETVANW DPHTMPVITE VSGFVRFTDM IDGQTITRQT
1060 1070 1080 1090 1100
DELTGLSSLV VLDSAERTAG GKDLRPALKI VDAQGNDVLI PGTDMPAQYF
1110 1120 1130 1140 1150
LPGKAIVQLE DGVQISSGDT LARIPQESGG TKDITGGLPR VADLFEARRP
1160 1170 1180 1190 1200
KEPAILAEIS GIVSFGKETK GKRRLVITPV DGSDPYEEMI PKWRQLNVFE
1210 1220 1230 1240 1250
GERVERGDVI SDGPEAPHDI LRLRGVHAVT RYIVNEVQDV YRLQGVKIND
1260 1270 1280 1290 1300
KHIEVIVRQM LRKATIVNAG SSDFLEGEQV EYSRVKIANR ELEANGKVGA
1310 1320 1330 1340 1350
TYSRDLLGIT KASLATESFI SAASFQETTR VLTEAAVAGK RDELRGLKEN
1360 1370 1380 1390 1400
VIVGRLIPAG TGYAYHQDRM RRRAAGEAPA APQVTAEDAS ASLAELLNAG

LGGSDNE
Length:1,407
Mass (Da):155,160
Last modified:July 21, 1986 - v1
Checksum:iA6878C61D15F4961
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1133 – 117745DITGG…RRLVI → ASPVVCRALRTCSKHVVRKS RQSWLKSAVSFPSVKKPKVN VVWLS (PubMed:6278450).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00339 Genomic DNA. Translation: CAA23626.1.
U00006 Genomic DNA. Translation: AAC43086.1.
U00096 Genomic DNA. Translation: AAC76962.1.
AP009048 Genomic DNA. Translation: BAE77332.1.
M38293 Genomic DNA. Translation: AAA24582.1.
M38303 Genomic DNA. Translation: AAA24584.1. Sequence problems.
V00340 Genomic DNA. Translation: CAA23628.1.
M38305 Genomic DNA. Translation: AAA24586.1.
M38288 Genomic DNA. Translation: AAA24408.1.
PIRiA00695. RNECC.
RefSeqiNP_418415.1. NC_000913.3.
WP_000653944.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76962; AAC76962; b3988.
BAE77332; BAE77332; BAE77332.
GeneIDi948487.
KEGGiecj:JW3951.
eco:b3988.
PATRICi32123497. VBIEscCol129921_4101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00339 Genomic DNA. Translation: CAA23626.1.
U00006 Genomic DNA. Translation: AAC43086.1.
U00096 Genomic DNA. Translation: AAC76962.1.
AP009048 Genomic DNA. Translation: BAE77332.1.
M38293 Genomic DNA. Translation: AAA24582.1.
M38303 Genomic DNA. Translation: AAA24584.1. Sequence problems.
V00340 Genomic DNA. Translation: CAA23628.1.
M38305 Genomic DNA. Translation: AAA24586.1.
M38288 Genomic DNA. Translation: AAA24408.1.
PIRiA00695. RNECC.
RefSeqiNP_418415.1. NC_000913.3.
WP_000653944.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AUKX-ray2.30A/B/C/D/E944-1129[»]
2LMCNMR-B1151-1213[»]
3IYDelectron microscopy-D1-1407[»]
3LU0electron microscopy-D1-1407[»]
4IQZX-ray2.10A/B/C/D/E932-1141[»]
4JK1X-ray3.90D/I1-1407[»]
4JK2X-ray4.20D/I1-1407[»]
4KMUX-ray3.85D/I1-1407[»]
4KN4X-ray3.96D/I1-1407[»]
4KN7X-ray3.69D/I1-1407[»]
4MEXX-ray3.90D/J1-1407[»]
4MEYX-ray3.95D/J1-1407[»]
4XSXX-ray3.71D/J1-1407[»]
4XSYX-ray4.01D/J1-1407[»]
4XSZX-ray3.68D/J1-1407[»]
4YG2X-ray3.70D/J1-1407[»]
4YLNX-ray5.50D/J/P1-1407[»]
4YLOX-ray6.00D/J/P1-1407[»]
4YLPX-ray5.50D/J/P1-1407[»]
4ZH2X-ray4.20D/J1-1407[»]
4ZH3X-ray4.08D/J1-1407[»]
4ZH4X-ray3.99D/J1-1407[»]
5BYHX-ray3.76D1-1407[»]
5EZKX-ray8.50D1-1407[»]
5IPLX-ray3.60D1-1407[»]
5IPMX-ray4.20D1-1407[»]
5IPNX-ray4.61D1-1407[»]
ProteinModelPortaliP0A8T7.
SMRiP0A8T7. Positions 15-1376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262959. 22 interactions.
DIPiDIP-35803N.
IntActiP0A8T7. 113 interactions.
MINTiMINT-1220667.
STRINGi511145.b3988.

Chemistry

BindingDBiP0A8T7.
ChEMBLiCHEMBL2364672.
DrugBankiDB00615. Rifabutin.
DB01045. Rifampicin.

Proteomic databases

EPDiP0A8T7.
PaxDbiP0A8T7.
PRIDEiP0A8T7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76962; AAC76962; b3988.
BAE77332; BAE77332; BAE77332.
GeneIDi948487.
KEGGiecj:JW3951.
eco:b3988.
PATRICi32123497. VBIEscCol129921_4101.

Organism-specific databases

EchoBASEiEB0888.
EcoGeneiEG10895. rpoC.

Phylogenomic databases

eggNOGiENOG4105D27. Bacteria.
COG0086. LUCA.
HOGENOMiHOG000218386.
InParanoidiP0A8T7.
KOiK03046.
OMAiYDLVNQN.
PhylomeDBiP0A8T7.

Enzyme and pathway databases

BioCyciEcoCyc:RPOC-MONOMER.
ECOL316407:JW3951-MONOMER.
MetaCyc:RPOC-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8T7.
PROiP0A8T7.

Family and domain databases

HAMAPiMF_01322. RNApol_bact_RpoC. 1 hit.
InterProiIPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]
PfamiPF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 2 hits.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]
SMARTiSM00663. RPOLA_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02386. rpoC_TIGR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPOC_ECOLI
AccessioniPrimary (citable) accession number: P0A8T7
Secondary accession number(s): P00577
, P00578, P78134, Q2M8S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.