P0A8T7 (RPOC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Synonyms:	tabB
Ordered Locus Names:	b3988, JW3951

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	1407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP-Rule MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
PTM	Acetylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay PubMed 16858726. Source: UniProtKB membrane Inferred from direct assay PubMed 16858726. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
htpG	P0A6Z3	2	EBI-543604,EBI-369221
rpoB	P0A8V2	7	EBI-543604,EBI-544996
rpoD	P00579	8	EBI-543604,EBI-545104

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1407

1407

DNA-directed RNA polymerase subunit beta' HAMAP-Rule MF_01322

PRO_0000067741

Amino acid modifications

Modified residue

983

1

N6-acetyllysine Ref.11

Experimental info

Sequence conflict

1133 – 1177

45

DITGG…RRLVI → ASPVVCRALRTCSKHVVRKS RQSWLKSAVSFPSVKKPKVN VVWLS Ref.10

Secondary structure

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1407

Helix Strand Turn

Details...

Helix

945 – 948

4

Beta strand

949 – 951

3

Beta strand

956 – 962

7

Beta strand

965 – 967

3

Beta strand

973 – 975

3

Beta strand

981 – 985

5

Beta strand

991 – 996

6

Beta strand

1002 – 1005

4

Beta strand

1016 – 1019

4

Beta strand

1022 – 1028

7

Beta strand

1033 – 1039

7

Turn

1043 – 1045

3

Beta strand

1046 – 1049

4

Turn

1052 – 1054

3

Beta strand

1058 – 1061

4

Helix

1064 – 1066

3

Helix

1071 – 1073

3

Beta strand

1077 – 1081

5

Beta strand

1093 – 1096

4

Beta strand

1098 – 1100

3

Beta strand

1106 – 1109

4

Beta strand

1120 – 1125

6

Beta strand

1155 – 1166

12

Beta strand

1169 – 1171

3

Beta strand

1174 – 1183

10

Beta strand

1186 – 1189

4

Beta strand

1202 – 1204

3

Beta strand

1209 – 1211

3

Sequences

	Sequence		Length	Mass (Da)
	P0A8T7 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: A6878C61D15F4961	FASTA	1,407	155,160
	10 20 30 40 50 60 MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR 70 80 90 100 110 120 IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQTKVRRE RMGHIELASP TAHIWFLKSL 130 140 150 160 170 180 PSRIGLLLDM PLRDIERVLY FESYVVIEGG MTNLERQQIL TEEQYLDALE EFGDEFDAKM 190 200 210 220 230 240 GAEAIQALLK SMDLEQECEQ LREELNETNS ETKRKKLTKR IKLLEAFVQS GNKPEWMILT 250 260 270 280 290 300 VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ 310 320 330 340 350 360 EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPY 370 380 390 400 410 420 LRLHQCGLPK KMALELFKPF IYGKLELRGL ATTIKAAKKM VEREEAVVWD ILDEVIREHP 430 440 450 460 470 480 VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLEAQLEA 490 500 510 520 530 540 RALMMSTNNI LSPANGEPII VPSQDVVLGL YYMTRDCVNA KGEGMVLTGP KEAERLYRSG 550 560 570 580 590 600 LASLHARVKV RITEYEKDAN GELVAKTSLK DTTVGRAILW MIVPKGLPYS IVNQALGKKA 610 620 630 640 650 660 ISKMLNTCYR ILGLKPTVIF ADQIMYTGFA YAARSGASVG IDDMVIPEKK HEIISEAEAE 670 680 690 700 710 720 VAEIQEQFQS GLVTAGERYN KVIDIWAAAN DRVSKAMMDN LQTETVINRD GQEEKQVSFN 730 740 750 760 770 780 SIYMMADSGA RGSAAQIRQL AGMRGLMAKP DGSIIETPIT ANFREGLNVL QYFISTHGAR 790 800 810 820 830 840 KGLADTALKT ANSGYLTRRL VDVAQDLVVT EDDCGTHEGI MMTPVIEGGD VKEPLRDRVL 850 860 870 880 890 900 GRVTAEDVLK PGTADILVPR NTLLHEQWCD LLEENSVDAV KVRSVVSCDT DFGVCAHCYG 910 920 930 940 950 960 RDLARGHIIN KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAAAESSI QVKNKGSIKL 970 980 990 1000 1010 1020 SNVKSVVNSS GKLVITSRNT ELKLIDEFGR TKESYKVPYG AVLAKGDGEQ VAGGETVANW 1030 1040 1050 1060 1070 1080 DPHTMPVITE VSGFVRFTDM IDGQTITRQT DELTGLSSLV VLDSAERTAG GKDLRPALKI 1090 1100 1110 1120 1130 1140 VDAQGNDVLI PGTDMPAQYF LPGKAIVQLE DGVQISSGDT LARIPQESGG TKDITGGLPR 1150 1160 1170 1180 1190 1200 VADLFEARRP KEPAILAEIS GIVSFGKETK GKRRLVITPV DGSDPYEEMI PKWRQLNVFE 1210 1220 1230 1240 1250 1260 GERVERGDVI SDGPEAPHDI LRLRGVHAVT RYIVNEVQDV YRLQGVKIND KHIEVIVRQM 1270 1280 1290 1300 1310 1320 LRKATIVNAG SSDFLEGEQV EYSRVKIANR ELEANGKVGA TYSRDLLGIT KASLATESFI 1330 1340 1350 1360 1370 1380 SAASFQETTR VLTEAAVAGK RDELRGLKEN VIVGRLIPAG TGYAYHQDRM RRRAAGEAPA 1390 1400 APQVTAEDAS ASLAELLNAG LGGSDNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit." Ovchinnikov Y.A., Monastyrskaya G.S., Gubanov V.V., Guryev S.O., Salomatina I.S., Shuvaeva T.M., Lipkin V.M., Sverdlov E.D. Nucleic Acids Res. 10:4035-4044(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, PARTIAL PROTEIN SEQUENCE.
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Primary structure of EcoRI-F fragment of rpoB, C genes and corresponding fragments of beta- and beta'-subunits of RNA polymerase from E.coli." Monastyrskaya G.S., Gubanov V.V., Guryev S.O., Lipkin V.M., Sverdlov E.D. Bioorg. Khim. 6:1106-1109(1980) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176.
[6]	"Structure of a central part of E.coli operon rpoBC. Nucleotide sequence of the gene for beta subunit of RNA polymerase." Gurevich A.I., Igoshin A.V., Kolosov M.N. Bioorg. Khim. 6:1580-1584(1980) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176.
[7]	"The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit." Ovchinnikov Y.A., Monastyrskaya G.S., Gubanov V.V., Guryev S.O., Chertov O.Y., Modyanov N.N., Grinkevich V.A., Makarova I.A., Marchenko T.V., Polovnikova I.N., Lipkin V.M., Sverdlov E.D. Eur. J. Biochem. 116:621-629(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-176, PROTEIN SEQUENCE OF 1-8.
[8]	"Primary structure of EcoRI-D fragment of rpoC gene and corresponding fragment of beta-subunit of RNA polymerase from E.coli." Monastyrskaya G.S., Guryev S.O., Kalinina N.F., Sorokin A.V., Salomatina I.S., Shuvaeva T.M., Lipkin V.M., Sverdlov E.D., Ovchinnikov Y.A. Bioorg. Khim. 8:130-134(1982) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 175-988.
[9]	"Primary structure of RNA polymerase from E.coli. Nucleotide sequence of E.coli DNA fragment containing a part of the rpoC gene and the corresponding C-terminal amino acid sequence of beta'-subunit." Ovchinnikov Y.A., Monastyrskaya G.S., Gubanov V.V., Salomatina I.S., Shuvaeva T.M., Lipkin V.M., Sverdlov E.D. Bioorg. Khim. 7:1107-1112(1981) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 987-1407.
[10]	"Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC)." Squires C., Krainer A., Barry G., Shen W.-F., Squires C.L. Nucleic Acids Res. 9:6827-6840(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1073-1407. Strain: K12.
[11]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-983, MASS SPECTROMETRY. Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
[12]	"Escherichia coli proteome analysis using the gene-protein database." VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C. Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY 2D-GEL.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00339 Genomic DNA. Translation: CAA23626.1.
U00006 Genomic DNA. Translation: AAC43086.1.
U00096 Genomic DNA. Translation: AAC76962.1.
AP009048 Genomic DNA. Translation: BAE77332.1.
M38293 Genomic DNA. Translation: AAA24582.1.
M38303 Genomic DNA. Translation: AAA24584.1. Sequence problems.
V00340 Genomic DNA. Translation: CAA23628.1.
M38305 Genomic DNA. Translation: AAA24586.1.
M38288 Genomic DNA. Translation: AAA24408.1.

PIR

RNECC. A00695.

RefSeq

NP_418415.1. NC_000913.2.
YP_491473.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AUK	X-ray	2.30	A/B/C/D/E	944-1129	[»]
2LMC	NMR	-	B	1151-1213	[»]
3IYD	electron microscopy	-	D	1-1407	[»]
3LU0	electron microscopy	-	D	1-1407	[»]
4IGC	X-ray	3.70	D/I	1-1407	[»]
4IQZ	X-ray	2.10	A/B/C/D/E	932-1140	[»]

ProteinModelPortal

P0A8T7.

SMR

P0A8T7. Positions 15-1382.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35803N.

IntAct

P0A8T7. 111 interactions.

MINT

MINT-1220667.

STRING

511145.b3988.

PTM databases

PhosSite

P010445.

Proteomic databases

PaxDb

P0A8T7.

PRIDE

P0A8T7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76962; AAC76962; b3988.
BAE77332; BAE77332; BAE77332.

GeneID

12933965.
948487.

KEGG

ecj:Y75_p3209.
eco:b3988.

PATRIC

32123497. VBIEscCol129921_4101.

Organism-specific databases

EchoBASE

EB0888.

EcoGene

EG10895. rpoC.

Phylogenomic databases

eggNOG

COG0086.

HOGENOM

HOG000218386.

KO

K03046.

OMA

DINDKHI.

ProtClustDB

PRK00566.

Enzyme and pathway databases

BioCyc

EcoCyc:RPOC-MONOMER.
ECOL316407:JW3951-MONOMER.
MetaCyc:RPOC-MONOMER.

Gene expression databases

Genevestigator

P0A8T7.

Family and domain databases

Gene3D

2.40.40.20. 2 hits.

HAMAP

MF_01322. RNApol_bact_RpoC.

InterPro

IPR009010. Asp_de-COase-like_dom.
IPR012754. DNA-dir_RpoC_beta_prime.
IPR000722. RNA_pol_asu.
IPR006592. RNA_pol_N.
IPR007080. RNA_pol_Rpb1_1.
IPR007066. RNA_pol_Rpb1_3.
IPR007083. RNA_pol_Rpb1_4.
IPR007081. RNA_pol_Rpb1_5.
[Graphical view]

Pfam

PF04997. RNA_pol_Rpb1_1. 1 hit.
PF00623. RNA_pol_Rpb1_2. 1 hit.
PF04983. RNA_pol_Rpb1_3. 1 hit.
PF05000. RNA_pol_Rpb1_4. 1 hit.
PF04998. RNA_pol_Rpb1_5. 1 hit.
[Graphical view]

SMART

SM00663. RPOLA_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02386. rpoC_TIGR. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A8T7.

Entry information

Entry name

RPOC_ECOLI

Accession

Primary (citable) accession number: P0A8T7
Secondary accession number(s): P00577 , P00578, P78134, Q2M8S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program