P0A8T1 (PRMA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein L11 methyltransferase
Short name=L11 Mtase
EC=2.1.1.-

Gene names

Name:	prmA
Synonyms:	yhdI
Ordered Locus Names:	b3259, JW3227

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methylates ribosomal protein L11. HAMAP MF_00735
Subcellular location	Cytoplasm HAMAP MF_00735.
Sequence similarities	Belongs to the methyltransferase superfamily. PrmA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal peptidyl-alanine trimethylation Inferred from mutant phenotype. Source: EcoCyc peptidyl-lysine trimethylation Inferred from direct assay. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein-lysine N-methyltransferase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplK	P0A7J7	3	EBI-556300,EBI-547288

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Ribosomal protein L11 methyltransferase HAMAP MF_00735		PRO_0000192257
Experimental info
	Mutagenesis	285	1	W → R: Great decrease in activity. Ref.6
	Sequence conflict	78	1	L → S in AAB28769. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A8T1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: CF0EDE130245B8FC	FASTA	293	31,877
	10 20 30 40 50 60 MPWIQLKLNT TGANAEDLSD ALMEAGAVSI TFQDTHDTPV FEPLPGETRL WGDTDVIGLF 70 80 90 100 110 120 DAETDMNDVV AILENHPLLG AGFAHKIEQL EDKDWEREWM DNFHPMRFGE RLWICPSWRD 130 140 150 160 170 180 VPDENAVNVM LDPGLAFGTG THPTTSLCLQ WLDSLDLTGK TVIDFGCGSG ILAIAALKLG 190 200 210 220 230 240 AAKAIGIDID PQAIQASRDN AERNGVSDRL ELYLPKDQPE EMKADVVVAN ILAGPLRELA 250 260 270 280 290 PLISVLPVSG GLLGLSGILA SQAESVCEAY ADSFALDPVV EKEEWCRITG RKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12." Vanet A., Plumbridge J.A., Alix J.-H. J. Bacteriol. 175:7178-7188(1993) [PubMed: 8226664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cloning, sequence, and expression of the pantothenate permease (panF) gene of Escherichia coli." Jackowski S., Alix J.-H. J. Bacteriol. 172:3842-3848(1990) [PubMed: 2193919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-4. Strain: K12.
[5]	"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli." Ball C.A., Osuna R., Ferguson K.C., Johnson R.C. J. Bacteriol. 174:8043-8056(1992) [PubMed: 1459953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 140-293.
[6]	"Ribosomal protein methylation in Escherichia coli: the gene prmA, encoding the ribosomal protein L11 methyltransferase, is dispensable." Vanet A., Plumbridge J.A., Guerin M.F., Alix J.-H. Mol. Microbiol. 14:947-958(1994) [PubMed: 7715456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 279-293, MUTAGENESIS OF TRP-285.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S67010 Genomic DNA. Translation: AAB28769.1. U18997 Genomic DNA. Translation: AAA58062.1. Frameshift. U18997 Genomic DNA. Translation: AAA58063.1. Frameshift. U00096 Genomic DNA. Translation: AAC76291.1. AP009048 Genomic DNA. Translation: BAE77300.1. M30953 Genomic DNA. No translation available. M95784 Genomic DNA. Translation: AAA23781.1.
PIR	E65118.
RefSeq	NP_417725.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A8T1.
SMR	P0A8T1. Positions 4-292.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47862N.
IntAct	P0A8T1. 13 interactions.
MINT	MINT-1232840.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004290; EBESCP00000004290; EBESCG00000003499. EBESCT00000014672; EBESCP00000013963; EBESCG00000013733.
GeneID	947708.
GenomeReviews	Gene locus JW3227 in contig AP009048_GR. Gene locus b3259 in contig U00096_GR.
KEGG	ecj:JW3227. eco:b3259.
PATRIC	32121948. VBIEscCol129921_3358.
Organism-specific databases
EchoBASE	EB1460.
EcoGene	EG11497. prmA.
Phylogenomic databases
eggNOG	COG2264.
GeneTree	EBGT00050000010752.
HOGENOM	HBG584653.
OMA	GTRLWIC.
PhylomeDB	P0A8T1.
ProtClustDB	PRK00517.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11497-MONOMER. MetaCyc:EG11497-MONOMER.
Gene expression databases
Genevestigator	P0A8T1.
Family and domain databases
HAMAP	MF_00735. Methyltr_PrmA. [Tree]
InterPro	IPR010456. Ribosomal-L11_MeTrfase_PrmA. IPR004498. Ribosomal_PrmA_MeTrfase. [Graphical view]
KO	K02687.
PANTHER	PTHR18895:SF3. PTHR18895:SF3. 1 hit.
Pfam	PF06325. PrmA. 1 hit. [Graphical view]
PIRSF	PIRSF000401. RPL11_MTase. 1 hit.
TIGRFAMs	TIGR00406. PrmA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRMA_ECOLI

Accession

Primary (citable) accession number: P0A8T1
Secondary accession number(s): P28637 , P76680, P76681, Q2M8V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program