Reviewed, UniProtKB/Swiss-Prot P0A8T1 (PRMA_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribosomal protein L11 methyltransferase
      Short name=L11 Mtase
    EC=2.1.1.-

Gene names

Name:	prmA
Synonyms:	yhdI
Ordered Locus Names:	b3259, JW3227

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Methylates ribosomal protein L11. HAMAP MF_00735
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the methyltransferase superfamily. PrmA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid methylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplK	P0A7J7	1	EBI-556300,EBI-547288

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Ribosomal protein L11 methyltransferase HAMAP MF_00735		PRO_0000192257
Experimental info
	Mutagenesis	285	1	W → R: Great decrease in activity. Ref.6
	Sequence conflict	78	1	L → S in AAB28769. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A8T1-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: CF0EDE130245B8FC	FASTA	293	31,877
	10 20 30 40 50 60 MPWIQLKLNT TGANAEDLSD ALMEAGAVSI TFQDTHDTPV FEPLPGETRL WGDTDVIGLF 70 80 90 100 110 120 DAETDMNDVV AILENHPLLG AGFAHKIEQL EDKDWEREWM DNFHPMRFGE RLWICPSWRD 130 140 150 160 170 180 VPDENAVNVM LDPGLAFGTG THPTTSLCLQ WLDSLDLTGK TVIDFGCGSG ILAIAALKLG 190 200 210 220 230 240 AAKAIGIDID PQAIQASRDN AERNGVSDRL ELYLPKDQPE EMKADVVVAN ILAGPLRELA 250 260 270 280 290 PLISVLPVSG GLLGLSGILA SQAESVCEAY ADSFALDPVV EKEEWCRITG RKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cotranscription of two genes necessary for ribosomal protein L11 methylation (prmA) and pantothenate transport (panF) in Escherichia coli K-12." Vanet A., Plumbridge J.A., Alix J.-H. J. Bacteriol. 175:7178-7188(1993) [PubMed: 8226664] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cloning, sequence, and expression of the pantothenate permease (panF) gene of Escherichia coli." Jackowski S., Alix J.-H. J. Bacteriol. 172:3842-3848(1990) [PubMed: 2193919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-4. Strain: K12.
[5]	"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli." Ball C.A., Osuna R., Ferguson K.C., Johnson R.C. J. Bacteriol. 174:8043-8056(1992) [PubMed: 1459953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 140-293.
[6]	"Ribosomal protein methylation in Escherichia coli: the gene prmA, encoding the ribosomal protein L11 methyltransferase, is dispensable." Vanet A., Plumbridge J.A., Guerin M.F., Alix J.-H. Mol. Microbiol. 14:947-958(1994) [PubMed: 7715456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 279-293, MUTAGENESIS OF TRP-285.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S67010 Genomic DNA. Translation: AAB28769.1. U18997 Genomic DNA. Translation: AAA58062.1. Frameshift. U18997 Genomic DNA. Translation: AAA58063.1. Frameshift. U00096 Genomic DNA. Translation: AAC76291.1. AP009048 Genomic DNA. Translation: BAE77300.1. M30953 Genomic DNA. No translation available. M95784 Genomic DNA. Translation: AAA23781.1.
PIR	E65118.
RefSeq	AP_003799.1. NP_417725.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A8T1. 13 interactions.
STRING	P0A8T1.
Genome annotation databases
GeneID	947708.
GenomeReviews	Gene locus JW3227 in contig AP009048_GR. Gene locus b3259 in contig U00096_GR.
KEGG	ecj:JW3227. eco:b3259.
Organism-specific databases
EchoBASE	EB1460.
EcoGene	EG11497. prmA.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A8T1.
OMA	EREEWCR
Enzyme and pathway databases
BioCyc	EcoCyc:EG11497-MON. ECOL168927:B3259-MON.
Gene expression databases
Genevestigator	P0A8T1.
Family and domain databases
HAMAP	MF_00735. [Tree]
InterPro	IPR010456. PrmA_MeTrfase. IPR004498. Ribosomal_PrmA_MeTrfase. [Graphical view]
Pfam	PF06325. PrmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00406. prmA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRMA_ECOLI

Accession

Primary (citable) accession number: P0A8T1
Secondary accession number(s): P28637 , P76680, P76681, Q2M8V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 24, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)