Flagellar transcriptional regulator FlhD - Escherichia coli (strain K12)

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P0A8S9 (FLHD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Flagellar transcriptional regulator FlhD

Gene names

Name:	flhD
Synonyms:	flbB
Ordered Locus Names:	b1892, JW1881

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways. Ref.5 Ref.8 Ref.10 Ref.11
Subunit structure	Homodimer; disulfide-linked. Forms a heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers. Ref.14
Subcellular location	Cytoplasm HAMAP-Rule MF_00725.
Induction	Expression is regulated by a large number of systems, including induction by quorum sensing via the two-component regulatory system QseB/QseC, induction by cAMP-CRP, repression by high osmolarity via OmpR and repression by H-NS. Ref.6 Ref.7 Ref.9
Domain	The C-terminal region contains a putative helix-turn-helix (HTH) motif, suggesting that this region may bind DNA. Ref.13
Miscellaneous	Has been reported to be involved in cell division regulation, but it was later shown (Ref.12) that this is not the case. HAMAP-Rule MF_00725
Sequence similarities	Belongs to the FlhD family.
Mass spectrometry	Molecular mass is 13317 Da from positions 1 - 116. Ref.7
Sequence caution	The sequence AAA23787.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAA15713.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Bacterial flagellum biogenesis Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	flagellum assembly Inferred from electronic annotation. Source: InterPro positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro regulation of flagellum assembly Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from direct assay Ref.5. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 116

116

Flagellar transcriptional regulator FlhD HAMAP-Rule MF_00725

PRO_0000182713

Amino acid modifications

Disulfide bond

Interchain HAMAP-Rule MF_00725

Experimental info

Mutagenesis

H → A: Partial swarming phenotype. Ref.8

Mutagenesis

D → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

F → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

R → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

N → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

S → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. Ref.8

Mutagenesis

R → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. Ref.8

Mutagenesis

V → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. Ref.8

Mutagenesis

H → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

T → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

I → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Mutagenesis

L → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Secondary structure

116

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A8S9 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 593287239E9A4C16
Show »

FASTA

116

13,316

        10         20         30         40         50         60 
MHTSELLKHI YDINLSYLLL AQRLIVQDKA SAMFRLGINE EMATTLAALT LPQMVKLAET 

        70         80         90        100        110 
NQLVCHFRFD SHQTITQLTQ DSRVDDLQQI HTGIMLSTRL LNDVNQPEEA LRKKRA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5' consensus sequences of operons under FlbB and FlaI control." Bartlett D.H., Frantz B.B., Matsumura P. J. Bacteriol. 170:1575-1581(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons." Liu X., Matsumura P. J. Bacteriol. 176:7345-7351(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FLHC, DNA-BINDING. Strain: K12 / MC1000 / ATCC 39531.
[6]	"Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR." Shin S., Park C. J. Bacteriol. 177:4696-4702(1995) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12.
[7]	"Multiple control of flagellum biosynthesis in Escherichia coli: role of H-NS protein and the cyclic AMP-catabolite activator protein complex in transcription of the flhDC master operon." Soutourina O., Kolb A., Krin E., Laurent-Winter C., Rimsky S., Danchin A., Bertin P. J. Bacteriol. 181:7500-7508(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY, INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[8]	"Extensive alanine scanning reveals protein-protein and protein-DNA interaction surfaces in the global regulator FlhD from Escherichia coli." Campos A., Matsumura P. Mol. Microbiol. 39:581-594(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH FLHC, MUTAGENESIS OF HIS-2; ASP-28; PHE-34; ARG-35; ASN-61; SER-82; ARG-83; VAL-84; HIS-91; THR-92; ILE-94 AND LEU-96. Strain: K12 / YK410.
[9]	"Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli." Sperandio V., Torres A.G., Kaper J.B. Mol. Microbiol. 43:809-821(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12.
[10]	"Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2." Stafford G.P., Ogi T., Hughes C. Microbiology 151:1779-1788(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DNA-BINDING. Strain: K12 / MC1000 / ATCC 39531.
[11]	"Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli." Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R. Genes Dev. 22:2434-2446(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574 and K12 / W3110 / ATCC 27325 / DSM 5911.
[12]	"Mutations in the flhD gene of Escherichia coli K-12 do not cause the reported effect on cell division." Siegele D.A., Bain S., Mao W. FEMS Microbiol. Lett. 309:94-99(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SHOWS THAT IT DOES NOT REGULATE CELL DIVISION. Strain: K12.
[13]	"Crystal structure of the global regulator FlhD from Escherichia coli at 1.8 A resolution." Campos A., Zhang R.G., Alkire R.W., Matsumura P., Westbrook E.M. Mol. Microbiol. 39:567-580(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-116, DOMAIN.
[14]	"Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription." Wang S., Fleming R.T., Westbrook E.M., Matsumura P., McKay D.B. J. Mol. Biol. 355:798-808(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M19439 Genomic DNA. Translation: AAA23787.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74962.2.
AP009048 Genomic DNA. Translation: BAA15713.1. Different initiation.

PIR

XMECFB. A27735.

RefSeq

NP_416406.4. NC_000913.2.
YP_490154.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G8E	X-ray	1.80	A/B	1-116	[»]
2AVU	X-ray	3.00	A/B/C/D	1-116	[»]
4ES4	X-ray	2.90	B/D/F/H	1-116	[»]

ProteinModelPortal

P0A8S9.

SMR

P0A8S9. Positions 3-106.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A8S9. 14 interactions.

STRING

511145.b1892.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74962; AAC74962; b1892.
BAA15713; BAA15713; BAA15713.

GeneID

12933247.
945442.

KEGG

ecj:Y75_p1868.
eco:b1892.

PATRIC

32119111. VBIEscCol129921_1973.

Organism-specific databases

EchoBASE

EB0316.

EcoGene

EG10320. flhD.

Phylogenomic databases

eggNOG

NOG07455.

HOGENOM

HOG000254676.

K02403.

OMA

ETNQLIC.

ProtClustDB

PRK02909.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10320-MONOMER.
ECOL316407:JW1881-MONOMER.

Gene expression databases

Genevestigator

P0A8S9.

Family and domain databases

HAMAP

MF_00725. FlhD.

InterPro

IPR023559. Flagellar_FlhD.
IPR007911. Flagellar_FlhD_dom.
[Graphical view]

Pfam

PF05247. FlhD. 1 hit.
[Graphical view]

ProDom

PD015582. Flagellar_transcriptional_act. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF63592. Flagellar_transcriptional_act. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A8S9.

Entry information

Entry name

FLHD_ECOLI

Accession

Primary (citable) accession number: P0A8S9
Secondary accession number(s): P11164

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents