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Protein

Flagellar transcriptional regulator FlhD

Gene

flhD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.4 Publications

Miscellaneous

Has been reported to be involved in cell division regulation, but it was later shown that this is not the case.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processBacterial flagellum biogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10320-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar transcriptional regulator FlhD
Gene namesi
Name:flhD
Synonyms:flbB
Ordered Locus Names:b1892, JW1881
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10320. flhD.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2H → A: Partial swarming phenotype. 1 Publication1
Mutagenesisi28D → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi34F → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi35R → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi61N → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi82S → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. 1 Publication1
Mutagenesisi83R → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. 1 Publication1
Mutagenesisi84V → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. 1 Publication1
Mutagenesisi91H → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi92T → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi94I → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1
Mutagenesisi96L → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001827131 – 116Flagellar transcriptional regulator FlhDAdd BLAST116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi65Interchain

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0A8S9.
PRIDEiP0A8S9.

Expressioni

Inductioni

Expression is regulated by a large number of systems, including induction by quorum sensing via the two-component regulatory system QseB/QseC, induction by cAMP-CRP, repression by high osmolarity via OmpR and repression by H-NS.3 Publications

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers.1 Publication

Protein-protein interaction databases

BioGridi4262241. 7 interactors.
DIPiDIP-9646N.
IntActiP0A8S9. 14 interactors.
STRINGi316385.ECDH10B_2033.

Structurei

Secondary structure

1116
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 27Combined sources24
Helixi29 – 36Combined sources8
Helixi40 – 47Combined sources8
Helixi51 – 58Combined sources8
Beta strandi59 – 62Combined sources4
Beta strandi64 – 68Combined sources5
Helixi72 – 78Combined sources7
Helixi83 – 91Combined sources9
Helixi94 – 97Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G8EX-ray1.80A/B1-116[»]
2AVUX-ray3.00A/B/C/D1-116[»]
4ES4X-ray2.90B/D/F/H1-116[»]
ProteinModelPortaliP0A8S9.
SMRiP0A8S9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8S9.

Family & Domainsi

Domaini

The C-terminal region contains a putative helix-turn-helix (HTH) motif, suggesting that this region may bind DNA.1 Publication

Sequence similaritiesi

Belongs to the FlhD family.Curated

Phylogenomic databases

eggNOGiENOG4105GEH. Bacteria.
ENOG4111JRF. LUCA.
HOGENOMiHOG000254676.
KOiK02403.

Family and domain databases

HAMAPiMF_00725. FlhD. 1 hit.
InterProiView protein in InterPro
IPR023559. Flagellar_FlhD.
PfamiView protein in Pfam
PF05247. FlhD. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD015582. Flagellar_transcriptional_act. 1 hit.
SUPFAMiSSF63592. SSF63592. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTSELLKHI YDINLSYLLL AQRLIVQDKA SAMFRLGINE EMATTLAALT
60 70 80 90 100
LPQMVKLAET NQLVCHFRFD SHQTITQLTQ DSRVDDLQQI HTGIMLSTRL
110
LNDVNQPEEA LRKKRA
Length:116
Mass (Da):13,316
Last modified:June 7, 2005 - v1
Checksum:i593287239E9A4C16
GO

Sequence cautioni

The sequence AAA23787 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA15713 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Mass spectrometryi

Molecular mass is 13317 Da from positions 1 - 116. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19439 Genomic DNA. Translation: AAA23787.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74962.2.
AP009048 Genomic DNA. Translation: BAA15713.1. Different initiation.
PIRiA27735. XMECFB.
RefSeqiNP_416406.4. NC_000913.3.
WP_001295647.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74962; AAC74962; b1892.
BAA15713; BAA15713; BAA15713.
GeneIDi945442.
KEGGiecj:JW1881.
eco:b1892.
PATRICifig|1411691.4.peg.355.

Similar proteinsi

Entry informationi

Entry nameiFLHD_ECOLI
AccessioniPrimary (citable) accession number: P0A8S9
Secondary accession number(s): P11164
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families