Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P0A8S9

- FLHD_ECOLI

UniProt

P0A8S9 - FLHD_ECOLI

Protein

Flagellar transcriptional regulator FlhD

Gene

flhD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.4 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. bacterial-type flagellum assembly Source: InterPro
    2. positive regulation of transcription, DNA-templated Source: InterPro
    3. regulation of bacterial-type flagellum assembly Source: UniProtKB-HAMAP
    4. transcription, DNA-templated Source: EcoCyc

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Bacterial flagellum biogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10320-MONOMER.
    ECOL316407:JW1881-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flagellar transcriptional regulator FlhD
    Gene namesi
    Name:flhD
    Synonyms:flbB
    Ordered Locus Names:b1892, JW1881
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10320. flhD.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21H → A: Partial swarming phenotype. 1 Publication
    Mutagenesisi28 – 281D → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi34 – 341F → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi35 – 351R → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi61 – 611N → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi82 – 821S → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. 1 Publication
    Mutagenesisi83 – 831R → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. 1 Publication
    Mutagenesisi84 – 841V → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. 1 Publication
    Mutagenesisi91 – 911H → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi92 – 921T → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi94 – 941I → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication
    Mutagenesisi96 – 961L → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 116116Flagellar transcriptional regulator FlhDPRO_0000182713Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi65 – 65Interchain

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    Expression is regulated by a large number of systems, including induction by quorum sensing via the two-component regulatory system QseB/QseC, induction by cAMP-CRP, repression by high osmolarity via OmpR and repression by H-NS.3 Publications

    Gene expression databases

    GenevestigatoriP0A8S9.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers.1 Publication

    Protein-protein interaction databases

    DIPiDIP-9646N.
    IntActiP0A8S9. 14 interactions.
    STRINGi511145.b1892.

    Structurei

    Secondary structure

    1
    116
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 2724
    Helixi29 – 368
    Helixi40 – 478
    Helixi51 – 588
    Beta strandi59 – 624
    Beta strandi64 – 685
    Helixi72 – 787
    Helixi83 – 919
    Helixi94 – 974

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G8EX-ray1.80A/B1-116[»]
    2AVUX-ray3.00A/B/C/D1-116[»]
    4ES4X-ray2.90B/D/F/H1-116[»]
    ProteinModelPortaliP0A8S9.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A8S9.

    Family & Domainsi

    Domaini

    The C-terminal region contains a putative helix-turn-helix (HTH) motif, suggesting that this region may bind DNA.1 Publication

    Sequence similaritiesi

    Belongs to the FlhD family.Curated

    Phylogenomic databases

    eggNOGiNOG07455.
    HOGENOMiHOG000254676.
    KOiK02403.
    OMAiCQFRFND.
    OrthoDBiEOG64JFRM.

    Family and domain databases

    HAMAPiMF_00725. FlhD.
    InterProiIPR023559. Flagellar_FlhD.
    [Graphical view]
    PfamiPF05247. FlhD. 1 hit.
    [Graphical view]
    ProDomiPD015582. Flagellar_transcriptional_act. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF63592. SSF63592. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A8S9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHTSELLKHI YDINLSYLLL AQRLIVQDKA SAMFRLGINE EMATTLAALT    50
    LPQMVKLAET NQLVCHFRFD SHQTITQLTQ DSRVDDLQQI HTGIMLSTRL 100
    LNDVNQPEEA LRKKRA 116
    Length:116
    Mass (Da):13,316
    Last modified:June 7, 2005 - v1
    Checksum:i593287239E9A4C16
    GO

    Sequence cautioni

    The sequence AAA23787.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence BAA15713.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Mass spectrometryi

    Molecular mass is 13317 Da from positions 1 - 116. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19439 Genomic DNA. Translation: AAA23787.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC74962.2.
    AP009048 Genomic DNA. Translation: BAA15713.1. Different initiation.
    PIRiA27735. XMECFB.
    RefSeqiNP_416406.4. NC_000913.3.
    YP_490154.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74962; AAC74962; b1892.
    BAA15713; BAA15713; BAA15713.
    GeneIDi12933247.
    945442.
    KEGGiecj:Y75_p1868.
    eco:b1892.
    PATRICi32119111. VBIEscCol129921_1973.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19439 Genomic DNA. Translation: AAA23787.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC74962.2 .
    AP009048 Genomic DNA. Translation: BAA15713.1 . Different initiation.
    PIRi A27735. XMECFB.
    RefSeqi NP_416406.4. NC_000913.3.
    YP_490154.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G8E X-ray 1.80 A/B 1-116 [» ]
    2AVU X-ray 3.00 A/B/C/D 1-116 [» ]
    4ES4 X-ray 2.90 B/D/F/H 1-116 [» ]
    ProteinModelPortali P0A8S9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9646N.
    IntActi P0A8S9. 14 interactions.
    STRINGi 511145.b1892.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74962 ; AAC74962 ; b1892 .
    BAA15713 ; BAA15713 ; BAA15713 .
    GeneIDi 12933247.
    945442.
    KEGGi ecj:Y75_p1868.
    eco:b1892.
    PATRICi 32119111. VBIEscCol129921_1973.

    Organism-specific databases

    EchoBASEi EB0316.
    EcoGenei EG10320. flhD.

    Phylogenomic databases

    eggNOGi NOG07455.
    HOGENOMi HOG000254676.
    KOi K02403.
    OMAi CQFRFND.
    OrthoDBi EOG64JFRM.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10320-MONOMER.
    ECOL316407:JW1881-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A8S9.
    PROi P0A8S9.

    Gene expression databases

    Genevestigatori P0A8S9.

    Family and domain databases

    HAMAPi MF_00725. FlhD.
    InterProi IPR023559. Flagellar_FlhD.
    [Graphical view ]
    Pfami PF05247. FlhD. 1 hit.
    [Graphical view ]
    ProDomi PD015582. Flagellar_transcriptional_act. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF63592. SSF63592. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5' consensus sequences of operons under FlbB and FlaI control."
      Bartlett D.H., Frantz B.B., Matsumura P.
      J. Bacteriol. 170:1575-1581(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons."
      Liu X., Matsumura P.
      J. Bacteriol. 176:7345-7351(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH FLHC, DNA-BINDING.
      Strain: K12 / MC1000 / ATCC 39531.
    6. "Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR."
      Shin S., Park C.
      J. Bacteriol. 177:4696-4702(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12.
    7. "Multiple control of flagellum biosynthesis in Escherichia coli: role of H-NS protein and the cyclic AMP-catabolite activator protein complex in transcription of the flhDC master operon."
      Soutourina O., Kolb A., Krin E., Laurent-Winter C., Rimsky S., Danchin A., Bertin P.
      J. Bacteriol. 181:7500-7508(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY, INDUCTION.
      Strain: K12 / MG1655 / ATCC 47076.
    8. "Extensive alanine scanning reveals protein-protein and protein-DNA interaction surfaces in the global regulator FlhD from Escherichia coli."
      Campos A., Matsumura P.
      Mol. Microbiol. 39:581-594(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH FLHC, MUTAGENESIS OF HIS-2; ASP-28; PHE-34; ARG-35; ASN-61; SER-82; ARG-83; VAL-84; HIS-91; THR-92; ILE-94 AND LEU-96.
      Strain: K12 / YK410.
    9. "Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli."
      Sperandio V., Torres A.G., Kaper J.B.
      Mol. Microbiol. 43:809-821(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12.
    10. "Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2."
      Stafford G.P., Ogi T., Hughes C.
      Microbiology 151:1779-1788(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.
      Strain: K12 / MC1000 / ATCC 39531.
    11. "Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli."
      Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R.
      Genes Dev. 22:2434-2446(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574 and K12 / W3110 / ATCC 27325 / DSM 5911.
    12. "Mutations in the flhD gene of Escherichia coli K-12 do not cause the reported effect on cell division."
      Siegele D.A., Bain S., Mao W.
      FEMS Microbiol. Lett. 309:94-99(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SHOWS THAT IT DOES NOT REGULATE CELL DIVISION.
      Strain: K12.
    13. "Crystal structure of the global regulator FlhD from Escherichia coli at 1.8 A resolution."
      Campos A., Zhang R.G., Alkire R.W., Matsumura P., Westbrook E.M.
      Mol. Microbiol. 39:567-580(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-116, DOMAIN.
    14. "Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription."
      Wang S., Fleming R.T., Westbrook E.M., Matsumura P., McKay D.B.
      J. Mol. Biol. 355:798-808(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiFLHD_ECOLI
    AccessioniPrimary (citable) accession number: P0A8S9
    Secondary accession number(s): P11164
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Has been reported to be involved in cell division regulation, but it was later shown that this is not the case.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3