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P0A8S9 (FLHD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flagellar transcriptional regulator FlhD
Gene names
Name:flhD
Synonyms:flbB
Ordered Locus Names:b1892, JW1881
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length116 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways. Ref.5 Ref.8 Ref.10 Ref.11

Subunit structure

Homodimer; disulfide-linked. Forms a heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers. Ref.14

Subcellular location

Cytoplasm HAMAP-Rule MF_00725.

Induction

Expression is regulated by a large number of systems, including induction by quorum sensing via the two-component regulatory system QseB/QseC, induction by cAMP-CRP, repression by high osmolarity via OmpR and repression by H-NS. Ref.6 Ref.7 Ref.9

Domain

The C-terminal region contains a putative helix-turn-helix (HTH) motif, suggesting that this region may bind DNA. Ref.13

Miscellaneous

Has been reported to be involved in cell division regulation, but it was later shown (Ref.12) that this is not the case.

Sequence similarities

Belongs to the FlhD family.

Mass spectrometry

Molecular mass is 13317 Da from positions 1 - 116. Ref.7

Sequence caution

The sequence AAA23787.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAA15713.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 116116Flagellar transcriptional regulator FlhD HAMAP-Rule MF_00725
PRO_0000182713

Amino acid modifications

Disulfide bond65Interchain HAMAP-Rule MF_00725

Experimental info

Mutagenesis21H → A: Partial swarming phenotype. Ref.8
Mutagenesis281D → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis341F → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis351R → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis611N → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis821S → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. Ref.8
Mutagenesis831R → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. Ref.8
Mutagenesis841V → A: Partial swarming phenotype. Does not affect FlhD/FlhC complex formation, but affects DNA binding. Ref.8
Mutagenesis911H → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis921T → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis941I → A: Non-swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8
Mutagenesis961L → A: Partial swarming phenotype. Affects FlhD/FlhC complex formation. Ref.8

Secondary structure

.................. 116
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A8S9 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 593287239E9A4C16

FASTA11613,316
        10         20         30         40         50         60 
MHTSELLKHI YDINLSYLLL AQRLIVQDKA SAMFRLGINE EMATTLAALT LPQMVKLAET 

        70         80         90        100        110 
NQLVCHFRFD SHQTITQLTQ DSRVDDLQQI HTGIMLSTRL LNDVNQPEEA LRKKRA 

« Hide

References

« Hide 'large scale' references
[1]"Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5' consensus sequences of operons under FlbB and FlaI control."
Bartlett D.H., Frantz B.B., Matsumura P.
J. Bacteriol. 170:1575-1581(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons."
Liu X., Matsumura P.
J. Bacteriol. 176:7345-7351(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FLHC, DNA-BINDING.
Strain: K12 / MC1000 / ATCC 39531.
[6]"Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR."
Shin S., Park C.
J. Bacteriol. 177:4696-4702(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K12.
[7]"Multiple control of flagellum biosynthesis in Escherichia coli: role of H-NS protein and the cyclic AMP-catabolite activator protein complex in transcription of the flhDC master operon."
Soutourina O., Kolb A., Krin E., Laurent-Winter C., Rimsky S., Danchin A., Bertin P.
J. Bacteriol. 181:7500-7508(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY, INDUCTION.
Strain: K12 / MG1655 / ATCC 47076.
[8]"Extensive alanine scanning reveals protein-protein and protein-DNA interaction surfaces in the global regulator FlhD from Escherichia coli."
Campos A., Matsumura P.
Mol. Microbiol. 39:581-594(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FLHC, MUTAGENESIS OF HIS-2; ASP-28; PHE-34; ARG-35; ASN-61; SER-82; ARG-83; VAL-84; HIS-91; THR-92; ILE-94 AND LEU-96.
Strain: K12 / YK410.
[9]"Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli."
Sperandio V., Torres A.G., Kaper J.B.
Mol. Microbiol. 43:809-821(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
Strain: K12.
[10]"Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2."
Stafford G.P., Ogi T., Hughes C.
Microbiology 151:1779-1788(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
Strain: K12 / MC1000 / ATCC 39531.
[11]"Inverse regulatory coordination of motility and curli-mediated adhesion in Escherichia coli."
Pesavento C., Becker G., Sommerfeldt N., Possling A., Tschowri N., Mehlis A., Hengge R.
Genes Dev. 22:2434-2446(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574 and K12 / W3110 / ATCC 27325 / DSM 5911.
[12]"Mutations in the flhD gene of Escherichia coli K-12 do not cause the reported effect on cell division."
Siegele D.A., Bain S., Mao W.
FEMS Microbiol. Lett. 309:94-99(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SHOWS THAT IT DOES NOT REGULATE CELL DIVISION.
Strain: K12.
[13]"Crystal structure of the global regulator FlhD from Escherichia coli at 1.8 A resolution."
Campos A., Zhang R.G., Alkire R.W., Matsumura P., Westbrook E.M.
Mol. Microbiol. 39:567-580(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 18-116, DOMAIN.
[14]"Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription."
Wang S., Fleming R.T., Westbrook E.M., Matsumura P., McKay D.B.
J. Mol. Biol. 355:798-808(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19439 Genomic DNA. Translation: AAA23787.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74962.2.
AP009048 Genomic DNA. Translation: BAA15713.1. Different initiation.
PIRXMECFB. A27735.
RefSeqNP_416406.4. NC_000913.3.
YP_490154.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G8EX-ray1.80A/B1-116[»]
2AVUX-ray3.00A/B/C/D1-116[»]
4ES4X-ray2.90B/D/F/H1-116[»]
ProteinModelPortalP0A8S9.
SMRP0A8S9. Positions 3-106.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9646N.
IntActP0A8S9. 14 interactions.
STRING511145.b1892.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74962; AAC74962; b1892.
BAA15713; BAA15713; BAA15713.
GeneID12933247.
945442.
KEGGecj:Y75_p1868.
eco:b1892.
PATRIC32119111. VBIEscCol129921_1973.

Organism-specific databases

EchoBASEEB0316.
EcoGeneEG10320. flhD.

Phylogenomic databases

eggNOGNOG07455.
HOGENOMHOG000254676.
KOK02403.
OMAETNQLIC.
OrthoDBEOG64JFRM.
ProtClustDBPRK02909.

Enzyme and pathway databases

BioCycEcoCyc:EG10320-MONOMER.
ECOL316407:JW1881-MONOMER.

Gene expression databases

GenevestigatorP0A8S9.

Family and domain databases

HAMAPMF_00725. FlhD.
InterProIPR023559. Flagellar_FlhD.
[Graphical view]
PfamPF05247. FlhD. 1 hit.
[Graphical view]
ProDomPD015582. Flagellar_transcriptional_act. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF63592. SSF63592. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A8S9.
PROP0A8S9.

Entry information

Entry nameFLHD_ECOLI
AccessionPrimary (citable) accession number: P0A8S9
Secondary accession number(s): P11164
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene