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Protein

HTH-type transcriptional regulator ArgP

Gene

argP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls the transcription of genes involved in arginine and lysine metabolism. Activates transcription of several genes, including argO, lysP, lysC, asd, dapB, dapD, lysA, gdhA and argK. Acts by binding directly to their promoter or control region (PubMed:10600368, PubMed:15150242, PubMed:17504942, PubMed:21441513, PubMed:21890697). ArgP dimer by itself is able to bind the argO promoter-operator region to form a binary complex, but the formation of a ternary complex with RNA polymerase is greatly stimulated only in presence of a coeffector. Both arginine and lysine are coeffectors at the argO promoter, but only arginine is competent to activate transcription. Lysine has repressive effects (PubMed:15150242, PubMed:17504942). ArgP also mediates lysine repression of dapB, and gdhA in vivo, but via an alternative mechanism: ArgP binding is directly reduced upon the addition of lysine (PubMed:21890697). Binds in vitro to the promoter region of dnaA and to the upstream region of the nrd promoter, but these genes are probably not regulated by ArgP in vivo (PubMed:9254708, PubMed:9819053, PubMed:21890697). In vitro, binds also to the three 13-mers located in the origin region (oriC) and blocks the initiation of replication (PubMed:1733927).8 Publications

Enzyme regulationi

Specifically cleaved by the protease DegP. Cleaved ArgP can no longer interact with the oriC region in vitro. Cleavage may play an important role in the control of the protein availability.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi21 – 4020H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  • bent DNA binding Source: EcoliWiki
  • sequence-specific DNA binding Source: EcoliWiki
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • negative regulation of DNA-dependent DNA replication initiation Source: EcoCyc
  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10490-MONOMER.
ECOL316407:JW2883-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator ArgPUniRule annotationCurated
Alternative name(s):
Inhibitor of chromosome initiation1 Publication
OriC replication inhibitor
Gene namesi
Name:argP1 PublicationUniRule annotation
Synonyms:iciA1 Publication
Ordered Locus Names:b2916, JW2883
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10490. argP.

Pathology & Biotechi

Disruption phenotypei

Null mutants are more sensitive to canavanine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297HTH-type transcriptional regulator ArgPPRO_0000105641Add
BLAST

Proteomic databases

EPDiP0A8S1.
PaxDbiP0A8S1.
PRIDEiP0A8S1.

Expressioni

Developmental stagei

The amount of protein increases in the late logarithmic phase.1 Publication

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4263054. 80 interactions.
DIPiDIP-48015N.
IntActiP0A8S1. 8 interactions.
MINTiMINT-1262794.
STRINGi511145.b2916.

Structurei

3D structure databases

ProteinModelPortaliP0A8S1.
SMRiP0A8S1. Positions 9-286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6057HTH lysR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CXD. Bacteria.
COG0583. LUCA.
HOGENOMiHOG000219802.
InParanoidiP0A8S1.
KOiK05596.
OMAiSTCCMIP.
PhylomeDBiP0A8S1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00513. HTH_type_ArgP. 1 hit.
InterProiIPR017685. ArgP.
IPR023490. ArgP_Gammaproteobact.
IPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR03298. argP. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRPDYRTLQ ALDAVIRERG FERAAQKLCI TQSAVSQRIK QLENMFGQPL
60 70 80 90 100
LVRTVPPRPT EQGQKLLALL RQVELLEEEW LGDEQTGSTP LLLSLAVNAD
110 120 130 140 150
SLATWLLPAL APVLADSPIR LNLQVEDETR TQERLRRGEV VGAVSIQHQA
160 170 180 190 200
LPSCLVDKLG ALDYLFVSSK PFAEKYFPNG VTRSALLKAP VVAFDHLDDM
210 220 230 240 250
HQAFLQQNFD LPPGSVPCHI VNSSEAFVQL ARQGTTCCMI PHLQIEKELA
260 270 280 290
SGELIDLTPG LFQRRMLYWH RFAPESRMMR KVTDALLDYG HKVLRQD
Length:297
Mass (Da):33,472
Last modified:June 7, 2005 - v1
Checksum:i8AD60B7B6661E3EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62865 Genomic DNA. Translation: AAA62780.1.
X66836 Genomic DNA. Translation: CAA47310.1.
U28377 Genomic DNA. Translation: AAA69083.1.
U00096 Genomic DNA. Translation: AAC75953.1.
AP009048 Genomic DNA. Translation: BAE76980.1.
X73026 Genomic DNA. Translation: CAA51508.1.
PIRiS22098.
RefSeqiNP_417391.1. NC_000913.3.
WP_000828351.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75953; AAC75953; b2916.
BAE76980; BAE76980; BAE76980.
GeneIDi944867.
KEGGiecj:JW2883.
eco:b2916.
PATRICi32121246. VBIEscCol129921_3011.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62865 Genomic DNA. Translation: AAA62780.1.
X66836 Genomic DNA. Translation: CAA47310.1.
U28377 Genomic DNA. Translation: AAA69083.1.
U00096 Genomic DNA. Translation: AAC75953.1.
AP009048 Genomic DNA. Translation: BAE76980.1.
X73026 Genomic DNA. Translation: CAA51508.1.
PIRiS22098.
RefSeqiNP_417391.1. NC_000913.3.
WP_000828351.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A8S1.
SMRiP0A8S1. Positions 9-286.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263054. 80 interactions.
DIPiDIP-48015N.
IntActiP0A8S1. 8 interactions.
MINTiMINT-1262794.
STRINGi511145.b2916.

Proteomic databases

EPDiP0A8S1.
PaxDbiP0A8S1.
PRIDEiP0A8S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75953; AAC75953; b2916.
BAE76980; BAE76980; BAE76980.
GeneIDi944867.
KEGGiecj:JW2883.
eco:b2916.
PATRICi32121246. VBIEscCol129921_3011.

Organism-specific databases

EchoBASEiEB0485.
EcoGeneiEG10490. argP.

Phylogenomic databases

eggNOGiENOG4105CXD. Bacteria.
COG0583. LUCA.
HOGENOMiHOG000219802.
InParanoidiP0A8S1.
KOiK05596.
OMAiSTCCMIP.
PhylomeDBiP0A8S1.

Enzyme and pathway databases

BioCyciEcoCyc:EG10490-MONOMER.
ECOL316407:JW2883-MONOMER.

Miscellaneous databases

PROiP0A8S1.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00513. HTH_type_ArgP. 1 hit.
InterProiIPR017685. ArgP.
IPR023490. ArgP_Gammaproteobact.
IPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR03298. argP. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGP_ECOLI
AccessioniPrimary (citable) accession number: P0A8S1
Secondary accession number(s): P24194, Q2M9S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.