Regulator of ribonuclease activity A - Escherichia coli (strain K12)

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P0A8R0 (RRAA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulator of ribonuclease activity A

Gene names

Name:	rraA
Synonyms:	menG, yiiV
Ordered Locus Names:	b3929, JW3900

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Globally modulates RNA abundance by binding to RNase E (Rne) and regulating its endonucleolytic activity. Can modulate Rne action in a substrate-dependent manner by altering the composition of the degradosome. Modulates RNA-binding and helicase activities of the degradosome. Ref.5 Ref.6 Ref.8 Ref.9 Ref.10
Subunit structure	Homotrimer. Forms a ring-like structure with a central cavity. Binds to both RNA-binding sites in the C-terminal region of Rne and to RhlB. Ref.5 Ref.8 Ref.10 Ref.11
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00471.
Induction	Induced upon entry into stationary phase, in a RpoS-dependent manner. Stability of the rraA transcript is Rne dependent, suggesting the existence of a feedback mechanism in the regulation of RraA level. Ref.7
Miscellaneous	RraA and RraB interact with Rne at separate sites within the Rne and exert distinct effects on the composition of the degradosome, affecting distinct sets of RNA transcripts. HAMAP-Rule MF_00471
Sequence similarities	Belongs to the RraA family.
Caution	Although it was initially thought to be a methyltransferase of the menaquinone pathway, Ref.5 showed that it has no SAM-dependent methyltransferase activity and is not involved in the menaquinone pathway.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from direct assay Ref.5. Source: EcoCyc negative regulation of endoribonuclease activity Inferred from direct assay Ref.5. Source: EcoCyc regulation of RNA metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	endoribonuclease inhibitor activity Inferred from electronic annotation. Source: HAMAP ribonuclease inhibitor activity Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 161

161

Regulator of ribonuclease activity A HAMAP-Rule MF_00471

PRO_0000209611

Secondary structure

161

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A8R0 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: B30371B838DE21F8
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FASTA

161

17,360

        10         20         30         40         50         60 
MKYDTSELCD IYQEDVNVVE PLFSNFGGRA SFGGQIITVK CFEDNGLLYD LLEQNGRGRV 

        70         80         90        100        110        120 
LVVDGGGSVR RALVDAELAR LAVQNEWEGL VIYGAVRQVD DLEELDIGIQ AMAAIPVGAA 

       130        140        150        160 
GEGIGESDVR VNFGGVTFFS GDHLYADNTG IILSEDPLDI E

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hudspeth M.E.S., Suvarna K., Meganathan R. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"RraA. a protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli." Lee K., Zhan X., Gao J., Qiu J., Feng Y., Meganathan R., Cohen S.N., Georgiou G. Cell 114:623-634(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RNE.
[6]	"RraA rescues Escherichia coli cells over-producing RNase E from growth arrest by modulating the ribonucleolytic activity." Yeom J.H., Lee K. Biochem. Biophys. Res. Commun. 345:1372-1376(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Regulation of RraA, a protein inhibitor of RNase E-mediated RNA decay." Zhao M., Zhou L., Kawarasaki Y., Georgiou G. J. Bacteriol. 188:3257-3263(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12.
[8]	"Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome." Gao J., Lee K., Zhao M., Qiu J., Zhan X., Saxena A., Moore C.J., Cohen S.N., Georgiou G. Mol. Microbiol. 61:394-406(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RNE. Strain: K12.
[9]	"Inhibitory effects of RraA and RraB on RNAse E-related enzymes imply conserved functions in the regulated enzymatic cleavage of RNA." Yeom J.H., Go H., Shin E., Kim H.L., Han S.H., Moore C.J., Bae J., Lee K. FEMS Microbiol. Lett. 285:10-15(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome." Gorna M.W., Pietras Z., Tsai Y.C., Callaghan A.J., Hernandez H., Robinson C.V., Luisi B.F. RNA 16:553-562(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RNE AND RHLB.
[11]	"The X-ray structure of Escherichia coli RraA (MenG), A protein inhibitor of RNA processing." Monzingo A.F., Gao J., Qiu J., Georgiou G., Robertus J.D. J. Mol. Biol. 332:1015-1024(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U56082 Genomic DNA. Translation: AAB01208.1.
L19201 Genomic DNA. Translation: AAB03061.1.
U00096 Genomic DNA. Translation: AAC76911.1.
AP009048 Genomic DNA. Translation: BAE77381.1.

PIR

S40872.

RefSeq

NP_418364.1. NC_000913.2.
YP_491522.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q5X	X-ray	2.00	A/B/C	1-161	[»]
2YJT	X-ray	2.90	A/B/C	1-161	[»]
2YJV	X-ray	2.80	A/B/C/D/E/F/G/H/I/J/K/L	1-161	[»]

ProteinModelPortal

P0A8R0.

SMR

P0A8R0. Positions 2-161.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-35864N.

IntAct

P0A8R0. 9 interactions.

STRING

511145.b3929.

Proteomic databases

PaxDb

P0A8R0.

PRIDE

P0A8R0.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76911; AAC76911; b3929.
BAE77381; BAE77381; BAE77381.

GeneID

12932791.
948419.

KEGG

ecj:Y75_p3258.
eco:b3929.

PATRIC

32123373. VBIEscCol129921_4047.

Organism-specific databases

EchoBASE

EB1825.

EcoGene

EG11879. rraA.

Phylogenomic databases

eggNOG

COG0684.

HOGENOM

HOG000252803.

K02553.

OMA

ELCDIYH.

ProtClustDB

PRK09372.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11879-MONOMER.
ECOL316407:JW3900-MONOMER.

Gene expression databases

Genevestigator

P0A8R0.

Family and domain databases

Gene3D

3.50.30.40. 1 hit.

HAMAP

MF_00471. RraA.

InterPro

IPR005493. RNase_E_inh/diMeMenaQ_MeTrfase.
IPR010203. RraA.
IPR014339. RraA_gpbac.
[Graphical view]

Pfam

PF03737. Methyltransf_6. 1 hit.
[Graphical view]

SUPFAM

SSF89562. RNaseE_inh/diMeMenaQ_MeTrfase. 1 hit.

TIGRFAMs

TIGR01935. NOT-MenG. 1 hit.
TIGR02998. RraA_entero. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A8R0.

Entry information

Entry name

RRAA_ECOLI

Accession

Primary (citable) accession number: P0A8R0
Secondary accession number(s): P32165, Q2M8M5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents