Reviewed, UniProtKB/Swiss-Prot P0A8Q6 (CLPS_ECOLI)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease adapter protein clpS

Gene names

Name:	clpS
Synonyms:	yljA
Ordered Locus Names:	b0881, JW0865

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the modulation of the specificity of the clpAP-mediated ATP-dependent protein degradation. HAMAP MF_00302
Subunit structure	Binds to the N-terminal domain of the chaperone ClpA. HAMAP MF_00302
Sequence similarities	Belongs to the clpS family.

Ontologies

Keywords
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Molecular function	protein binding Ref.6 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
clpA	P0ABH9	2	EBI-561456,EBI-546140

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 106

106

ATP-dependent Clp protease adapter protein clpS HAMAP MF_00302

PRO_0000215707

Secondary structure

1

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106

Helix Strand Turn

Details...

Beta strand

27 – 33

7

Beta strand

36 – 38

3

Helix

40 – 51

12

Helix

55 – 68

14

Beta strand

69 – 77

9

Helix

78 – 94

17

Beta strand

100 – 105

6

Sequences

	Sequence		Length	Mass (Da)
	P0A8Q6-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 2E9DC8000EA3CE86	FASTA	106	12,179
	10 20 30 40 50 60 MGKTNDWLDF DQLAEEKVRD ALKPPSMYKV ILVNDDYTPM EFVIDVLQKF FSYDVERATQ 70 80 90 100 LMLAVHYQGK AICGVFTAEV AETKVAMVNK YARENEHPLL CTLEKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"ClpS, a substrate modulator of the ClpAP machine." Dougan D.A., Reid B.G., Horwich A.L., Bukau B. Mol. Cell 9:673-683(2002) [PubMed: 11931773] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA." Zeth K., Ravelli R.B., Paal K., Cusack S., Bukau B., Dougan D.A. Nat. Struct. Biol. 9:906-911(2002) [PubMed: 12426582] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH CLPA.
[6]	"Crystal structure of the heterodimeric complex of the adaptor, ClpS, with the N-domain of the AAA+ chaperone, ClpA." Guo F., Esser L., Singh S.K., Maurizi M.R., Xia D. J. Biol. Chem. 277:46753-46762(2002) [PubMed: 12235156] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH CLPA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U00096 Genomic DNA. Translation: AAC73968.1.
AP009048 Genomic DNA. Translation: BAA35600.1.

PIR

A64827.

RefSeq

AP_001512.1.
NP_415402.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LZW	X-ray	2.50	A	1-106	[»]
1MBU	X-ray	2.30	C/D	1-106	[»]
1MBV	X-ray	3.30	B	1-106	[»]
1MBX	X-ray	2.25	C/D	1-106	[»]
1MG9	X-ray	2.30	A	1-106	[»]
1R6O	X-ray	2.25	C/D	1-106	[»]
1R6Q	X-ray	2.35	C/D	1-106	[»]
2W9R	X-ray	1.70	A	1-106	[»]
2WA8	X-ray	2.15	A/C	1-106	[»]
2WA9	X-ray	2.90	A/B/C/D/E/F/G	1-106	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A8Q6. 11 interactions.

STRING

P0A8Q6.

2-D gel databases

SWISS-2DPAGE

P0A8Q6.

Genome annotation databases

GeneID

948443.

GenomeReviews

Gene locus JW0865 in contig AP009048_GR.
Gene locus b0881 in contig U00096_GR.

KEGG

ecj:JW0865.
eco:b0881.

Organism-specific databases

EchoBASE

EB3992.

EcoGene

EG14241. clpS.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A8Q6.

OMA

LMNDDYT.

Enzyme and pathway databases

BioCyc

EcoCyc:G6463-MON.

Gene expression databases

Genevestigator

P0A8Q6.

Family and domain databases

HAMAP

MF_00302.
[Tree]

InterPro

IPR003769. ClpS_core.
IPR014719. Ribosomal_L7/12_C/ClpS-like.
[Graphical view]

Gene3D

G3DSA:3.30.1390.10. Ribosomal_L7/12_C/ClpS-like. 1 hit.

Pfam

PF02617. ClpS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CLPS_ECOLI

Accession

Primary (citable) accession number: P0A8Q6
Secondary accession number(s): P75832

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)