Fumarate reductase subunit D - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0A8Q3 (FRDD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Fumarate reductase subunit D

Alternative name(s):
Fumarate reductase 13 kDa hydrophobic protein

Gene names

Name:	frdD
Ordered Locus Names:	b4151, JW4112

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to be involved in the anchoring of the catalytic components of the fumarate reductase complex to the cytoplasmic membrane. HAMAP-Rule MF_00709
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein (FrdA), an iron-sulfur protein (FrdB), and two hydrophobic anchor proteins (FrdC and FrdD).
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_00709.
Induction	Regulated at the transcriptional level in response to the cellular availability of the alternate electron acceptors oxygen, nitrate, and fumarate. Ref.6
Sequence similarities	Belongs to the FrdD family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anaerobic respiration Inferred from mutant phenotype PubMed 14109218. Source: EcoCyc fermentation Inferred from mutant phenotype PubMed 14109218. Source: EcoCyc fumarate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from direct assay PubMed 8499449. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 119

119

Fumarate reductase subunit D HAMAP-Rule MF_00709

PRO_0000196542

Regions

Topological domain

1 – 8

Cytoplasmic Ref.8

Transmembrane

9 – 35

Helical HAMAP-Rule MF_00709

Topological domain

36 – 60

Periplasmic Ref.8

Transmembrane

61 – 89

Helical HAMAP-Rule MF_00709

Topological domain

90 – 96

Cytoplasmic Ref.8

Transmembrane

97 – 115

Helical HAMAP-Rule MF_00709

Topological domain

116 – 119

Periplasmic Ref.8

Secondary structure

119

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A8Q3 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: E9D119342B1D5357
Show »

FASTA

119

13,107

        10         20         30         40         50         60 
MINPNPKRSD EPVFWGLFGA GGMWSAIIAP VMILLVGILL PLGLFPGDAL SYERVLAFAQ 

        70         80         90        100        110 
SFIGRVFLFL MIVLPLWCGL HRMHHAMHDL KIHVPAGKWV FYGLAAILTV VTLIGVVTI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Overlap between ampC and frd operons on the Escherichia coli chromosome." Grundstroem T., Jaurin B. Proc. Natl. Acad. Sci. U.S.A. 79:1111-1115(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"ampC beta-lactamase hyperproduction in Escherichia coli: natural ampicillin resistance generated by horizontal chromosomal DNA transfer from Shigella." Olsson O., Bergstroem S., Lindberg F.P., Normark S. Proc. Natl. Acad. Sci. U.S.A. 80:7556-7560(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-119.
[6]	"Transcription of the Escherichia coli fumarate reductase genes (frdABCD) and their coordinate regulation by oxygen, nitrate, and fumarate." Jones H.M., Gunsalus R.P. J. Bacteriol. 164:1100-1109(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 109-119, INDUCTION.
[7]	"Replacement of the proximal heme thiolate ligand in chloroperoxidase with a histidine residue." Yi X., Mroczko M., Manoj K.M., Wang X., Hager L.P. Proc. Natl. Acad. Sci. U.S.A. 96:12412-12417(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF CYS-50.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
[9]	"Structure of the Escherichia coli fumarate reductase respiratory complex." Iverson T.M., Luna-Chavez C., Cecchini G., Rees D.C. Science 284:1961-1966(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).
[10]	"Crystallographic studies of the Escherichia coli quinol-fumarate reductase with inhibitors bound to the quinol-binding site." Iverson T.M., Luna-Chavez C., Croal L.R., Cecchini G., Rees D.C. J. Biol. Chem. 277:16124-16130(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01611 Genomic DNA. Translation: AAA23440.1.
V00277 Genomic DNA. Translation: CAA23536.1.
U14003 Genomic DNA. Translation: AAA97050.1.
U00096 Genomic DNA. Translation: AAC77111.1.
AP009048 Genomic DNA. Translation: BAE78155.1.
M11979 Genomic DNA. Translation: AAA23434.1.

PIR

WMEC13. A04431.

RefSeq

NP_418575.1. NC_000913.2.
YP_492296.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KF6	X-ray	2.70	D/P	1-119	[»]
1KFY	X-ray	3.60	D/P	1-119	[»]
1L0V	X-ray	3.30	D/P	1-119	[»]
2B76	X-ray	3.30	D/P	1-119	[»]
3CIR	X-ray	3.65	D/P	1-119	[»]
3P4P	X-ray	2.80	D/P	1-119	[»]
3P4Q	X-ray	3.35	D/P	1-119	[»]
3P4R	X-ray	3.05	D/P	1-119	[»]
3P4S	X-ray	3.10	D/P	1-119	[»]

ProteinModelPortal

P0A8Q3.

SMR

P0A8Q3. Positions 1-119.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A8Q3. 1 interaction.

STRING

511145.b4151.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC77111; AAC77111; b4151.
BAE78155; BAE78155; BAE78155.

GeneID

12933705.
948668.

KEGG

ecj:Y75_p4040.
eco:b4151.

PATRIC

32123875. VBIEscCol129921_4285.

Organism-specific databases

EchoBASE

EB0329.

EcoGene

EG10333. frdD.

Phylogenomic databases

eggNOG

COG3080.

HOGENOM

HOG000281495.

K00247.

OMA

GKIACYA.

ProtClustDB

PRK05470.

Enzyme and pathway databases

BioCyc

EcoCyc:FUM-MEMB2.
ECOL316407:JW4112-MONOMER.
MetaCyc:FUM-MEMB2.

Gene expression databases

Genevestigator

P0A8Q3.

Family and domain databases

HAMAP

MF_00709. Fumarate_red_D.

InterPro

IPR003418. Fumarate_red_D.
[Graphical view]

Pfam

PF02313. Fumarate_red_D. 1 hit.
[Graphical view]

PIRSF

PIRSF000179. FrdD. 1 hit.

ProDom

PD015693. Fumarate_red_D. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A8Q3.

Entry information

Entry name

FRDD_ECOLI

Accession

Primary (citable) accession number: P0A8Q3
Secondary accession number(s): P03806, Q2M6F1, Q47048

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents