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Protein

Tyrosine recombinase XerC

Gene

xerC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds cooperatively to specific DNA consensus sequences that are separated from XerD binding sites by a short central region, forming the heterotetrameric XerC-XerD complex that recombines DNA substrates. The complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids at ColE1 xer (or cer) and pSC101 (or psi) sites. In the complex XerC specifically exchanges the top DNA strands (By similarity).By similarity3 Publications

Enzyme regulationi

During recombination, the heterotetrameric complex catalyzes two consecutive pairs of strand exchanges, implying that specific pairs of active sites are sequentially switched on and off in the recombinase tetramer to ensure that appropriate DNA strands will be exchanged at both reaction steps. FtsK plays a central role in this catalytic state switch that turns recombinase on and off reciprocally. The reciprocal C-terminal interaction between XerC and XerD may also participate in the enzymatic switch process.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei148By similarity1
Active sitei172By similarity1
Active sitei240By similarity1
Active sitei243By similarity1
Active sitei266By similarity1
Active sitei275O-(3'-phospho-DNA)-tyrosine intermediate1

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • site-specific recombinase activity Source: EcoliWiki
  • tyrosine-based site-specific recombinase activity Source: EcoliWiki

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: UniProtKB-HAMAP
  • plasmid maintenance Source: EcoliWiki
  • plasmid recombination Source: EcoliWiki
  • resolution of recombination intermediates Source: EcoliWiki
  • transposition, DNA-mediated Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, DNA integration, DNA recombination

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11069-MONOMER.
ECOL316407:JW3784-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine recombinase XerC
Gene namesi
Name:xerC
Ordered Locus Names:b3811, JW3784
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11069. xerC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Holliday junction resolvase complex Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi243R → Q: Abolishes DNA cleavage activity. 1 Publication1
Mutagenesisi252 – 254ESS → NHG: Abolishes chromosomal recombination but not plasmid resolution. 1 Publication3
Mutagenesisi275Y → F: Abolishes DNA cleavage activity. 3 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000952941 – 298Tyrosine recombinase XerCAdd BLAST298

Proteomic databases

PaxDbiP0A8P6.
PRIDEiP0A8P6.

Interactioni

Subunit structurei

Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD, in which XerC interacts with XerD via its C-terminal region, XerD interacts with XerC via its C-terminal region and so on.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
araCP0A9E03EBI-1133806,EBI-1113479
rzpRP775512EBI-1133806,EBI-9135212

Protein-protein interaction databases

BioGridi4263112. 406 interactors.
DIPiDIP-726N.
IntActiP0A8P6. 9 interactors.
STRINGi511145.b3811.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DCFX-ray2.30A111-298[»]
ProteinModelPortaliP0A8P6.
SMRiP0A8P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108IYS. Bacteria.
COG4973. LUCA.
HOGENOMiHOG000045294.
InParanoidiP0A8P6.
KOiK03733.
OMAiERQVSPH.
PhylomeDBiP0A8P6.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
HAMAPiMF_01808. Recomb_XerC_XerD. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR010998. Integrase_Lambda-type_N.
IPR023109. Integrase_recombinase_N.
IPR004107. Integrase_SAM-like_N.
IPR011931. Recomb_XerC.
IPR023009. Tyrosine_recombinase_XerC/XerD.
[Graphical view]
PfamiPF02899. Phage_int_SAM_1. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.
TIGRFAMsiTIGR02224. recomb_XerC. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLHTDVER YLRYLSVERQ LSPITLLNYQ RQLEAIINFA SENGLQSWQQ
60 70 80 90 100
CDVTMVRNFA VRSRRKGLGA ASLALRLSAL RSFFDWLVSQ NELKANPAKG
110 120 130 140 150
VSAPKAPRHL PKNIDVDDMN RLLDIDINDP LAVRDRAMLE VMYGAGLRLS
160 170 180 190 200
ELVGLDIKHL DLESGEVWVM GKGSKERRLP IGRNAVAWIE HWLDLRDLFG
210 220 230 240 250
SEDDALFLSK LGKRISARNV QKRFAEWGIK QGLNNHVHPH KLRHSFATHM
260 270 280 290
LESSGDLRGV QELLGHANLS TTQIYTHLDF QHLASVYDAA HPRAKRGK
Length:298
Mass (Da):33,868
Last modified:June 7, 2005 - v1
Checksum:i91706E6945752FBA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184 – 185NA → KP in AAA67607 (PubMed:1379743).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38257 Genomic DNA. Translation: AAA24763.1.
M87049 Genomic DNA. Translation: AAA67607.1.
U00096 Genomic DNA. Translation: AAC76814.1.
AP009048 Genomic DNA. Translation: BAE77489.1.
PIRiC37841.
RefSeqiNP_418256.1. NC_000913.3.
WP_000130691.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76814; AAC76814; b3811.
BAE77489; BAE77489; BAE77489.
GeneIDi948355.
KEGGiecj:JW3784.
eco:b3811.
PATRICi32123123. VBIEscCol129921_3927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38257 Genomic DNA. Translation: AAA24763.1.
M87049 Genomic DNA. Translation: AAA67607.1.
U00096 Genomic DNA. Translation: AAC76814.1.
AP009048 Genomic DNA. Translation: BAE77489.1.
PIRiC37841.
RefSeqiNP_418256.1. NC_000913.3.
WP_000130691.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5DCFX-ray2.30A111-298[»]
ProteinModelPortaliP0A8P6.
SMRiP0A8P6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263112. 406 interactors.
DIPiDIP-726N.
IntActiP0A8P6. 9 interactors.
STRINGi511145.b3811.

Proteomic databases

PaxDbiP0A8P6.
PRIDEiP0A8P6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76814; AAC76814; b3811.
BAE77489; BAE77489; BAE77489.
GeneIDi948355.
KEGGiecj:JW3784.
eco:b3811.
PATRICi32123123. VBIEscCol129921_3927.

Organism-specific databases

EchoBASEiEB1062.
EcoGeneiEG11069. xerC.

Phylogenomic databases

eggNOGiENOG4108IYS. Bacteria.
COG4973. LUCA.
HOGENOMiHOG000045294.
InParanoidiP0A8P6.
KOiK03733.
OMAiERQVSPH.
PhylomeDBiP0A8P6.

Enzyme and pathway databases

BioCyciEcoCyc:EG11069-MONOMER.
ECOL316407:JW3784-MONOMER.

Miscellaneous databases

PROiP0A8P6.

Family and domain databases

Gene3Di1.10.150.130. 1 hit.
1.10.443.10. 1 hit.
HAMAPiMF_01808. Recomb_XerC_XerD. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013762. Integrase-like_cat.
IPR002104. Integrase_catalytic.
IPR010998. Integrase_Lambda-type_N.
IPR023109. Integrase_recombinase_N.
IPR004107. Integrase_SAM-like_N.
IPR011931. Recomb_XerC.
IPR023009. Tyrosine_recombinase_XerC/XerD.
[Graphical view]
PfamiPF02899. Phage_int_SAM_1. 1 hit.
PF00589. Phage_integrase. 1 hit.
[Graphical view]
SUPFAMiSSF47823. SSF47823. 1 hit.
SSF56349. SSF56349. 1 hit.
TIGRFAMsiTIGR02224. recomb_XerC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiXERC_ECOLI
AccessioniPrimary (citable) accession number: P0A8P6
Secondary accession number(s): P22885, Q2M8B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.