Leucyl/phenylalanyl-tRNA--protein transferase - Escherichia coli (strain K12)

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P0A8P1 (LFTR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Leucyl/phenylalanyl-tRNA--protein transferase
EC=2.3.2.6

Alternative name(s):
L/F-transferase
Leucyltransferase
Phenyalanyltransferase

Gene names

Name:	aat
Synonyms:	ycaA
Ordered Locus Names:	b0885, JW0868

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine. HAMAP-Rule MF_00688
Catalytic activity	L-leucyl-tRNA + protein = tRNA + L-leucyl-protein. HAMAP-Rule MF_00688 L-phenylalanyl-tRNA + protein = tRNA + L-phenylalanyl-protein. HAMAP-Rule MF_00688
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00688.
Sequence similarities	Belongs to the L/F-transferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process via the N-end rule pathway Inferred by curator PubMed 1962196. Source: EcoliWiki
Cellular_component	cytoplasm Inferred from direct assay PubMed 4909560. Source: EcoliWiki
Molecular_function	leucyltransferase activity Inferred from direct assay PubMed 4909560. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 234

234

Leucyl/phenylalanyl-tRNA--protein transferase HAMAP-Rule MF_00688

PRO_0000207217

Secondary structure

234

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A8P1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 8C725890D42ABF6F
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FASTA

234

26,619

        10         20         30         40         50         60 
MRLVQLSRHS IAFPSPEGAL REPNGLLALG GDLSPARLLM AYQRGIFPWF SPGDPILWWS 

        70         80         90        100        110        120 
PDPRAVLWPE SLHISRSMKR FHKRSPYRVT MNYAFGQVIE GCASDREEGT WITRGVVEAY 

       130        140        150        160        170        180 
HRLHELGHAH SIEVWREDEL VGGMYGVAQG TLFCGESMFS RMENASKTAL LVFCEEFIGH 

       190        200        210        220        230 
GGKLIDCQVL NDHTASLGAC EIPRRDYLNY LNQMRLGRLP NNFWVPRCLF SPQE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and expression of the infA operon encoding translational initiation factor IF1. Transcriptional control by growth rate." Cummings H.S., Sands J.F., Foreman P.C., Fraser J., Hershey J.W.B. J. Biol. Chem. 266:16491-16498(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat." Shrader T.E., Tobias J.W., Varshavsky A. J. Bacteriol. 175:4364-4374(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The leucyl/phenylalanyl-tRNA-protein transferase. Overexpression and characterization of substrate recognition, domain structure, and secondary structure." Abramochkin G., Shrader T.E. J. Biol. Chem. 270:20621-20628(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M63145 Genomic DNA. Translation: AAC36910.1.
L10383 Unassigned DNA. Translation: AAA03231.1.
U00096 Genomic DNA. Translation: AAC73971.1.
AP009048 Genomic DNA. Translation: BAA35605.1.

PIR

A36888.

RefSeq

NP_415405.1. NC_000913.2.
YP_489157.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CXA	X-ray	1.60	A	1-234	[»]
2DPS	X-ray	2.40	A/B	1-234	[»]
2DPT	X-ray	2.75	A/B	1-234	[»]
2Z3K	X-ray	2.85	A/B	2-234	[»]
2Z3L	X-ray	2.75	A/B	2-234	[»]
2Z3M	X-ray	2.70	A/B	2-234	[»]
2Z3N	X-ray	2.50	A/B	2-234	[»]
2Z3O	X-ray	2.40	A/B	2-234	[»]
2Z3P	X-ray	2.50	A/B	2-234	[»]

ProteinModelPortal

P0A8P1.

SMR

P0A8P1. Positions 2-233.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48235N.

IntAct

P0A8P1. 8 interactions.

STRING

511145.b0885.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73971; AAC73971; b0885.
BAA35605; BAA35605; BAA35605.

GeneID

12930994.
945490.

KEGG

ecj:Y75_p0857.
eco:b0885.

PATRIC

32116979. VBIEscCol129921_0914.

Organism-specific databases

EchoBASE

EB1103.

EcoGene

EG11112. aat.

Phylogenomic databases

eggNOG

COG2360.

HOGENOM

HOG000102325.

K00684.

OMA

MFYGESM.

ProtClustDB

PRK00301.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11112-MONOMER.
ECOL316407:JW0868-MONOMER.
MetaCyc:EG11112-MONOMER.

Gene expression databases

Genevestigator

P0A8P1.

Family and domain databases

HAMAP

MF_00688. Leu_Phe_trans.

InterPro

IPR016181. Acyl_CoA_acyltransferase.
IPR004616. Leu/Phe-tRNA_Trfase.
[Graphical view]

Pfam

PF03588. Leu_Phe_trans. 1 hit.
[Graphical view]

SUPFAM

SSF55729. Acyl_CoA_acyltransferase. 1 hit.

TIGRFAMs

TIGR00667. aat. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P0A8P1.

Entry information

Entry name

LFTR_ECOLI

Accession

Primary (citable) accession number: P0A8P1
Secondary accession number(s): P23885

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents