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P0A8P1

- LFTR_ECOLI

UniProt

P0A8P1 - LFTR_ECOLI

Protein

Leucyl/phenylalanyl-tRNA--protein transferase

Gene

aat

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine.

    Catalytic activityi

    L-leucyl-tRNA(Leu) + [protein] = tRNA(Leu) + L-leucyl-[protein].
    L-phenylalanyl-tRNA(Phe) + [protein] = tRNA + L-phenylalanyl-[protein].

    GO - Molecular functioni

    1. leucyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: EcoliWiki

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11112-MONOMER.
    ECOL316407:JW0868-MONOMER.
    MetaCyc:EG11112-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucyl/phenylalanyl-tRNA--protein transferase (EC:2.3.2.6)
    Alternative name(s):
    L/F-transferase
    Leucyltransferase
    Phenyalanyltransferase
    Gene namesi
    Name:aat
    Synonyms:ycaA
    Ordered Locus Names:b0885, JW0868
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11112. aat.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: EcoliWiki

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 234234Leucyl/phenylalanyl-tRNA--protein transferasePRO_0000207217Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0A8P1.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-48235N.
    IntActiP0A8P1. 10 interactions.
    STRINGi511145.b0885.

    Structurei

    Secondary structure

    1
    234
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 53
    Helixi16 – 183
    Turni21 – 255
    Beta strandi26 – 294
    Helixi35 – 439
    Beta strandi57 – 593
    Beta strandi62 – 676
    Helixi69 – 713
    Helixi76 – 838
    Beta strandi88 – 936
    Helixi95 – 1039
    Beta strandi106 – 1083
    Helixi109 – 1113
    Helixi114 – 12512
    Beta strandi128 – 1369
    Beta strandi139 – 14911
    Beta strandi152 – 16211
    Helixi165 – 17915
    Beta strandi184 – 1896
    Helixi192 – 1965
    Beta strandi200 – 2023
    Helixi204 – 21411
    Turni221 – 2244
    Beta strandi225 – 2306

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CXAX-ray1.60A1-234[»]
    2DPSX-ray2.40A/B1-234[»]
    2DPTX-ray2.75A/B1-234[»]
    2Z3KX-ray2.85A/B2-234[»]
    2Z3LX-ray2.75A/B2-234[»]
    2Z3MX-ray2.70A/B2-234[»]
    2Z3NX-ray2.50A/B2-234[»]
    2Z3OX-ray2.40A/B2-234[»]
    2Z3PX-ray2.50A/B2-234[»]
    ProteinModelPortaliP0A8P1.
    SMRiP0A8P1. Positions 2-233.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A8P1.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the L/F-transferase family.Curated

    Phylogenomic databases

    eggNOGiCOG2360.
    HOGENOMiHOG000102325.
    KOiK00684.
    OMAiWSPDPRG.
    OrthoDBiEOG6WX4R3.
    PhylomeDBiP0A8P1.

    Family and domain databases

    HAMAPiMF_00688. Leu_Phe_trans.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR004616. Leu/Phe-tRNA_Trfase.
    [Graphical view]
    PfamiPF03588. Leu_Phe_trans. 1 hit.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 1 hit.
    TIGRFAMsiTIGR00667. aat. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A8P1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLVQLSRHS IAFPSPEGAL REPNGLLALG GDLSPARLLM AYQRGIFPWF    50
    SPGDPILWWS PDPRAVLWPE SLHISRSMKR FHKRSPYRVT MNYAFGQVIE 100
    GCASDREEGT WITRGVVEAY HRLHELGHAH SIEVWREDEL VGGMYGVAQG 150
    TLFCGESMFS RMENASKTAL LVFCEEFIGH GGKLIDCQVL NDHTASLGAC 200
    EIPRRDYLNY LNQMRLGRLP NNFWVPRCLF SPQE 234
    Length:234
    Mass (Da):26,619
    Last modified:June 7, 2005 - v1
    Checksum:i8C725890D42ABF6F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63145 Genomic DNA. Translation: AAC36910.1.
    L10383 Unassigned DNA. Translation: AAA03231.1.
    U00096 Genomic DNA. Translation: AAC73971.1.
    AP009048 Genomic DNA. Translation: BAA35605.1.
    PIRiA36888.
    RefSeqiNP_415405.1. NC_000913.3.
    YP_489157.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73971; AAC73971; b0885.
    BAA35605; BAA35605; BAA35605.
    GeneIDi12930994.
    945490.
    KEGGiecj:Y75_p0857.
    eco:b0885.
    PATRICi32116979. VBIEscCol129921_0914.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63145 Genomic DNA. Translation: AAC36910.1 .
    L10383 Unassigned DNA. Translation: AAA03231.1 .
    U00096 Genomic DNA. Translation: AAC73971.1 .
    AP009048 Genomic DNA. Translation: BAA35605.1 .
    PIRi A36888.
    RefSeqi NP_415405.1. NC_000913.3.
    YP_489157.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CXA X-ray 1.60 A 1-234 [» ]
    2DPS X-ray 2.40 A/B 1-234 [» ]
    2DPT X-ray 2.75 A/B 1-234 [» ]
    2Z3K X-ray 2.85 A/B 2-234 [» ]
    2Z3L X-ray 2.75 A/B 2-234 [» ]
    2Z3M X-ray 2.70 A/B 2-234 [» ]
    2Z3N X-ray 2.50 A/B 2-234 [» ]
    2Z3O X-ray 2.40 A/B 2-234 [» ]
    2Z3P X-ray 2.50 A/B 2-234 [» ]
    ProteinModelPortali P0A8P1.
    SMRi P0A8P1. Positions 2-233.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48235N.
    IntActi P0A8P1. 10 interactions.
    STRINGi 511145.b0885.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73971 ; AAC73971 ; b0885 .
    BAA35605 ; BAA35605 ; BAA35605 .
    GeneIDi 12930994.
    945490.
    KEGGi ecj:Y75_p0857.
    eco:b0885.
    PATRICi 32116979. VBIEscCol129921_0914.

    Organism-specific databases

    EchoBASEi EB1103.
    EcoGenei EG11112. aat.

    Phylogenomic databases

    eggNOGi COG2360.
    HOGENOMi HOG000102325.
    KOi K00684.
    OMAi WSPDPRG.
    OrthoDBi EOG6WX4R3.
    PhylomeDBi P0A8P1.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11112-MONOMER.
    ECOL316407:JW0868-MONOMER.
    MetaCyc:EG11112-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A8P1.
    PROi P0A8P1.

    Gene expression databases

    Genevestigatori P0A8P1.

    Family and domain databases

    HAMAPi MF_00688. Leu_Phe_trans.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR004616. Leu/Phe-tRNA_Trfase.
    [Graphical view ]
    Pfami PF03588. Leu_Phe_trans. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 1 hit.
    TIGRFAMsi TIGR00667. aat. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression of the infA operon encoding translational initiation factor IF1. Transcriptional control by growth rate."
      Cummings H.S., Sands J.F., Foreman P.C., Fraser J., Hershey J.W.B.
      J. Biol. Chem. 266:16491-16498(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat."
      Shrader T.E., Tobias J.W., Varshavsky A.
      J. Bacteriol. 175:4364-4374(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The leucyl/phenylalanyl-tRNA-protein transferase. Overexpression and characterization of substrate recognition, domain structure, and secondary structure."
      Abramochkin G., Shrader T.E.
      J. Biol. Chem. 270:20621-20628(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12.

    Entry informationi

    Entry nameiLFTR_ECOLI
    AccessioniPrimary (citable) accession number: P0A8P1
    Secondary accession number(s): P23885
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3