P0A8N7 (YJEA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YjeA

Alternative name(s):
GX

Gene names

Name:	poxA
Synonyms:	genX, yjeA
Ordered Locus Names:	b4155, JW4116

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	With YjeK might be involved in the post-translational modification of elongation factor P (EF-P). Ref.8
Disruption phenotype	Cell have a reduced pyruvate oxidase activity and a reduced growth rate. Ref.6
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Caution	Was originally (Ref.1) suggested to be a tRNA synthase, however its lack of an anticodon binding domain makes this highly unlikely.
Sequence caution	The sequence AAA97054.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro lysine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Uncharacterized protein YjeA HAMAP MF_00174		PRO_0000152719
Experimental info
	Sequence conflict	78	1	E → Q in AAA23436. Ref.5
	Sequence conflict	144	1	A → E in AAA23436. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P0A8N7 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: F72B535CCA23E4CC	FASTA	325	36,976
	10 20 30 40 50 60 MSETASWQPS ASIPNLLKRA AIMAEIRRFF ADRGVLEVET PCMSQATVTD IHLVPFETRF 70 80 90 100 110 120 VGPGHSQGMN LWLMTSPEYH MKRLLVAGCG PVFQLCRSFR NEEMGRYHNP EFTMLEWYRP 130 140 150 160 170 180 HYDMYRLMNE VDDLLQQVLD CPAAESLSYQ QAFLRYLEID PLSADKTQLR EVAAKLDLSN 190 200 210 220 230 240 VADTEEDRDT LLQLLFTFGV EPNIGKEKPT FVYHFPASQA SLAQISTEDH RVAERFEVYY 250 260 270 280 290 300 KGIELANGFH ELTDAREQQQ RFEQDNRKRA ARGLPQHPID QNLIEALKVG MPDCSGVALG 310 320 VDRLVMLALG AETLAEVIAF SVDRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for a new Escherichia coli protein resembling a lysyl-tRNA synthetase." Kong L., Fromant M., Blanquet S., Plateau P. Gene 108:163-164(1991) [PubMed: 1761227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli." Cole S.T. Eur. J. Biochem. 122:479-484(1982) [PubMed: 7037404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-152.
[6]	"Mapping nonselectable genes of Escherichia coli by using transposon Tn10: location of a gene affecting pyruvate oxidase." Chang Y.-Y., Cronan J.E. Jr. J. Bacteriol. 151:1279-1289(1982) [PubMed: 6286595] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: K12.
[7]	"Homology of aspartyl- and lysyl-tRNA synthetases." Gampel A., Tzagoloff A. Proc. Natl. Acad. Sci. U.S.A. 86:6023-6027(1989) [PubMed: 2668951] [Abstract] Cited for: SIMILARITY TO CLASS-II AA-TRNA SYNTHETASES.
[8]	"Predicting the pathway involved in post-translational modification of Elongation factor P in a subset of bacterial species." Bailly M., de Crecy-Lagard V. Biol. Direct 5:3-3(2010) [PubMed: 20070887] [Abstract] Cited for: POSSIBLE FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59988 Genomic DNA. Translation: CAA42604.1. U14003 Genomic DNA. Translation: AAA97054.1. Different initiation. U00096 Genomic DNA. Translation: AAC77115.2. AP009048 Genomic DNA. Translation: BAE78159.1. J01611 Genomic DNA. Translation: AAA23436.2.
PIR	S56383.
RefSeq	NP_418579.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A8N7.
SMR	P0A8N7. Positions 5-325.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A8N7. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002581; EBESCP00000002581; EBESCG00000002105. EBESCT00000014713; EBESCP00000014004; EBESCG00000013774.
GeneID	948672.
GenomeReviews	Gene locus JW4116 in contig AP009048_GR. Gene locus b4155 in contig U00096_GR.
KEGG	ecj:JW4116. eco:b4155.
PATRIC	32123883. VBIEscCol129921_4289.
Organism-specific databases
EchoBASE	EB1196.
EcoGene	EG11211. poxA.
Phylogenomic databases
eggNOG	COG2269.
GeneTree	EBGT00050000009271.
HOGENOM	HBG631383.
OMA	MPEASGI.
PhylomeDB	P0A8N7.
ProtClustDB	PRK09350.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11211-MONOMER.
Gene expression databases
Genevestigator	P0A8N7.
Family and domain databases
HAMAP	MF_00174. YjeA. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004525. Lys-tRNA-synth-rel. IPR018149. Lys-tRNA-synth_II_C. [Graphical view]
KO	K04568.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF7. PTHR22594:SF7. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00462. GenX. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YJEA_ECOLI

Accession

Primary (citable) accession number: P0A8N7
Secondary accession number(s): P03812 , P78141, Q2M6E7, Q8XDP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program