Reviewed, UniProtKB/Swiss-Prot P0A8N7 (SYK3_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative lysyl-tRNA synthetase
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase
      Short name=LysRS
    GX

Gene names

Name:	poxA
Synonyms:	genX, yjeA
Ordered Locus Names:	b4155, JW4116

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could be a lysyl-tRNA synthetase. HAMAP MF_00174
Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00174
Disruption phenotype	Cell have a reduced pyruvate oxidase activity and a reduced growth rate. Ref.6
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
aceE	P0AFG8	1	EBI-562598,EBI-542683

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Putative lysyl-tRNA synthetase HAMAP MF_00174		PRO_0000152719
Experimental info
	Sequence conflict	78	1	E → Q in AAA23436. Ref.5
	Sequence conflict	144	1	A → E in AAA23436. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P0A8N7-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: F72B535CCA23E4CC	FASTA	325	36,976
	10 20 30 40 50 60 MSETASWQPS ASIPNLLKRA AIMAEIRRFF ADRGVLEVET PCMSQATVTD IHLVPFETRF 70 80 90 100 110 120 VGPGHSQGMN LWLMTSPEYH MKRLLVAGCG PVFQLCRSFR NEEMGRYHNP EFTMLEWYRP 130 140 150 160 170 180 HYDMYRLMNE VDDLLQQVLD CPAAESLSYQ QAFLRYLEID PLSADKTQLR EVAAKLDLSN 190 200 210 220 230 240 VADTEEDRDT LLQLLFTFGV EPNIGKEKPT FVYHFPASQA SLAQISTEDH RVAERFEVYY 250 260 270 280 290 300 KGIELANGFH ELTDAREQQQ RFEQDNRKRA ARGLPQHPID QNLIEALKVG MPDCSGVALG 310 320 VDRLVMLALG AETLAEVIAF SVDRA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for a new Escherichia coli protein resembling a lysyl-tRNA synthetase." Kong L., Fromant M., Blanquet S., Plateau P. Gene 108:163-164(1991) [PubMed: 1761227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli." Cole S.T. Eur. J. Biochem. 122:479-484(1982) [PubMed: 7037404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-152.
[6]	"Mapping nonselectable genes of Escherichia coli by using transposon Tn10: location of a gene affecting pyruvate oxidase." Chang Y.-Y., Cronan J.E. Jr. J. Bacteriol. 151:1279-1289(1982) [PubMed: 6286595] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: K12.
[7]	"Homology of aspartyl- and lysyl-tRNA synthetases." Gampel A., Tzagoloff A. Proc. Natl. Acad. Sci. U.S.A. 86:6023-6027(1989) [PubMed: 2668951] [Abstract] Cited for: SIMILARITY TO CLASS-II AA-TRNA SYNTHETASES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59988 Genomic DNA. Translation: CAA42604.1. U14003 Genomic DNA. Translation: AAA97054.1. Different initiation. U00096 Genomic DNA. Translation: AAC77115.1. AP009048 Genomic DNA. Translation: BAE78159.1. J01611 Genomic DNA. Translation: AAA23436.2.
PIR	S56383.
RefSeq	AP_004658.1. NP_418579.2.
3D structure databases
HSSP	HSSP built from PDB template 1E1O based on UniProtKB P14825.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A8N7. 3 interactions.
Genome annotation databases
GeneID	948672.
GenomeReviews	Gene locus JW4116 in contig AP009048_GR. Gene locus b4155 in contig U00096_GR.
KEGG	ecj:JW4116. eco:b4155.
Organism-specific databases
EchoBASE	EB1196.
EcoGene	EG11211. poxA.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A8N7.
OMA	P0A8N7. VFRNEEA.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11211-MON.
Family and domain databases
HAMAP	MF_00174. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004525. Lys-tRNA-synth-rel. IPR018149. Lys-tRNA-synth_II_C. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00462. genX. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK3_ECOLI

Accession

Primary (citable) accession number: P0A8N7
Secondary accession number(s): P03812 , P78141, Q2M6E7, Q8XDP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)