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Protein

Lysine--tRNA ligase, heat inducible

Gene

lysU

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Also can synthesize a number of adenyl dinucleotides (in particular AppppA). These dinucleotides have been proposed to act as modulators of the heat-shock response and stress response.

Miscellaneous

There are two lysyl-tRNA ligases in E.coli: lysS is expressed constitutively, while lysU is heat inducible.

Catalytic activityi

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).

Cofactori

Mg2+Note: Binds 3 Mg2+ ions per subunit. The third one is coordinated by ATP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi415Magnesium 11
Metal bindingi422Magnesium 11
Metal bindingi422Magnesium 21

GO - Molecular functioni

  • ATP binding Source: EcoliWiki
  • ligase activity Source: EcoliWiki
  • lysine-tRNA ligase activity Source: EcoCyc
  • magnesium ion binding Source: EcoliWiki
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • lysyl-tRNA aminoacylation Source: EcoCyc
  • tRNA aminoacylation for protein translation Source: EcoliWiki

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:LYSU-MONOMER
MetaCyc:LYSU-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Lysine--tRNA ligase, heat inducible (EC:6.1.1.6)
Alternative name(s):
Lysyl-tRNA synthetase
Short name:
LysRS
Gene namesi
Name:lysU
Ordered Locus Names:b4129, JW4090
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10553 lysU

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • membrane Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001526272 – 505Lysine--tRNA ligase, heat inducibleAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114N6-acetyllysine1 Publication1
Modified residuei156N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP0A8N5
PRIDEiP0A8N5

PTM databases

iPTMnetiP0A8N5

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4262688, 18 interactors
DIPiDIP-36212N
IntActiP0A8N5, 52 interactors
STRINGi316385.ECDH10B_4322

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 32Combined sources20
Helixi46 – 53Combined sources8
Helixi58 – 64Combined sources7
Beta strandi67 – 80Combined sources14
Beta strandi83 – 90Combined sources8
Beta strandi93 – 100Combined sources8
Turni101 – 103Combined sources3
Helixi108 – 111Combined sources4
Helixi113 – 115Combined sources3
Beta strandi121 – 130Combined sources10
Beta strandi136 – 147Combined sources12
Helixi165 – 168Combined sources4
Helixi170 – 176Combined sources7
Helixi178 – 199Combined sources22
Turni200 – 202Combined sources3
Beta strandi210 – 214Combined sources5
Beta strandi224 – 227Combined sources4
Turni228 – 231Combined sources4
Beta strandi232 – 236Combined sources5
Helixi241 – 250Combined sources10
Beta strandi254 – 262Combined sources9
Beta strandi273 – 284Combined sources12
Helixi287 – 306Combined sources20
Beta strandi307 – 313Combined sources7
Beta strandi316 – 319Combined sources4
Beta strandi325 – 328Combined sources4
Helixi329 – 336Combined sources8
Helixi342 – 346Combined sources5
Helixi348 – 357Combined sources10
Helixi368 – 379Combined sources12
Helixi381 – 383Combined sources3
Beta strandi388 – 391Combined sources4
Helixi395 – 397Combined sources3
Beta strandi410 – 418Combined sources9
Beta strandi421 – 429Combined sources9
Helixi433 – 448Combined sources16
Helixi459 – 468Combined sources10
Beta strandi471 – 478Combined sources8
Helixi479 – 486Combined sources8
Helixi492 – 494Combined sources3
Beta strandi496 – 498Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E1OX-ray2.12A2-505[»]
1E1TX-ray2.40A2-505[»]
1E22X-ray2.43A2-505[»]
1E24X-ray2.35A2-505[»]
1LYLX-ray2.80A/B/C2-505[»]
ProteinModelPortaliP0A8N5
SMRiP0A8N5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8N5

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CRK Bacteria
COG1190 LUCA
HOGENOMiHOG000236578
InParanoidiP0A8N5
KOiK04567
OMAiAYMSAIM
PhylomeDBiP0A8N5

Family and domain databases

CDDicd00775 LysRS_core, 1 hit
HAMAPiMF_00252 Lys_tRNA_synth_class2, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR002313 Lys-tRNA-ligase_II
IPR034762 Lys-tRNA-ligase_II_bac/euk
IPR018149 Lys-tRNA-synth_II_C
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PIRSFiPIRSF039101 LysRS2, 1 hit
PRINTSiPR00982 TRNASYNTHLYS
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00499 lysS_bact, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEQETRGAN EAIDFNDELR NRREKLAALR QQGVAFPNDF RRDHTSDQLH
60 70 80 90 100
EEFDAKDNQE LESLNIEVSV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA
110 120 130 140 150
RDSLPEGVYN DQFKKWDLGD IIGARGTLFK TQTGELSIHC TELRLLTKAL
160 170 180 190 200
RPLPDKFHGL QDQEVRYRQR YLDLIANDKS RQTFVVRSKI LAAIRQFMVA
210 220 230 240 250
RGFMEVETPM MQVIPGGASA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG
260 270 280 290 300
GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYHDL IELTESLFRT
310 320 330 340 350
LAQEVLGTTK VTYGEHVFDF GKPFEKLTMR EAIKKYRPET DMADLDNFDA
360 370 380 390 400
AKALAESIGI TVEKSWGLGR IVTEIFDEVA EAHLIQPTFI TEYPAEVSPL
410 420 430 440 450
ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAERF QEQVNAKAAG
460 470 480 490 500
DDEAMFYDED YVTALEYGLP PTAGLGIGID RMIMLFTNSH TIRDVILFPA

MRPQK
Length:505
Mass (Da):57,827
Last modified:January 23, 2007 - v2
Checksum:i547415F5875396E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125Missing in AAA24096 (PubMed:2188953).Curated1
Sequence conflicti236L → A in AAA24096 (PubMed:2188953).Curated1
Sequence conflicti258 – 262INRNF → HVT in AAA24096 (PubMed:2188953).Curated5
Sequence conflicti268 – 269SV → R in AAA24096 (PubMed:2188953).Curated2
Sequence conflicti351A → R in AAA24096 (PubMed:2188953).Curated1
Sequence conflicti371I → S in AAA24096 (PubMed:2188953).Curated1
Sequence conflicti380 – 384AEAHL → VEGHV in AAA24096 (PubMed:2188953).Curated5
Sequence conflicti388T → S in AAA24096 (PubMed:2188953).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16542 Genomic DNA Translation: CAA34542.1
M30630 Genomic DNA Translation: AAA24096.1
U14003 Genomic DNA Translation: AAA97029.1
U00096 Genomic DNA Translation: AAC77090.1
AP009048 Genomic DNA Translation: BAE78132.1
PIRiS56358 SYECKU
RefSeqiNP_418553.1, NC_000913.3
WP_001295074.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77090; AAC77090; b4129
BAE78132; BAE78132; BAE78132
GeneIDi948645
KEGGiecj:JW4090
eco:b4129
PATRICifig|1411691.4.peg.2570

Similar proteinsi

Entry informationi

Entry nameiSYK2_ECOLI
AccessioniPrimary (citable) accession number: P0A8N5
Secondary accession number(s): P14825, Q2M6H4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health