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Protein

Asparagine--tRNA ligase

Gene

asnS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

GO - Molecular functioni

  • asparagine-tRNA ligase activity Source: EcoCyc
  • ATP binding Source: EcoCyc
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • asparaginyl-tRNA aminoacylation Source: EcoCyc
  • tRNA aminoacylation for protein translation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ASNS-MONOMER.
ECOL316407:JW0913-MONOMER.
MetaCyc:ASNS-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligase (EC:6.1.1.22)
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name:
AsnRS
Gene namesi
Name:asnS
Synonyms:tss
Ordered Locus Names:b0930, JW0913
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10094. asnS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi426 – 4261Y → F: No effect.
Mutagenesisi426 – 4261Y → S: 15-fold increase in Km for ATP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 466465Asparagine--tRNA ligasePRO_0000176406Add
BLAST

Proteomic databases

EPDiP0A8M0.
PaxDbiP0A8M0.
PRIDEiP0A8M0.

2D gel databases

SWISS-2DPAGEP0A8M0.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-9179N.
IntActiP0A8M0. 3 interactions.
MINTiMINT-1319001.
STRINGi511145.b0930.

Structurei

3D structure databases

ProteinModelPortaliP0A8M0.
SMRiP0A8M0. Positions 19-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C5S. Bacteria.
COG0017. LUCA.
HOGENOMiHOG000226033.
InParanoidiP0A8M0.
KOiK01893.
OMAiPEDMEFF.
PhylomeDBiP0A8M0.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF6. PTHR22594:SF6. 2 hits.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVPVADVL QGRVAVDSEV TVRGWVRTRR DSKAGISFLA VYDGSCFDPV
60 70 80 90 100
QAVINNSLPN YNEDVLRLTT GCSVIVTGKV VASPGQGQQF EIQASKVEVA
110 120 130 140 150
GWVEDPDTYP MAAKRHSIEY LREVAHLRPR TNLIGAVARV RHTLAQALHR
160 170 180 190 200
FFNEQGFFWV STPLITASDT EGAGEMFRVS TLDLENLPRN DQGKVDFDKD
210 220 230 240 250
FFGKESFLTV SGQLNGETYA CALSKIYTFG PTFRAENSNT SRHLAEFWML
260 270 280 290 300
EPEVAFANLN DIAGLAEAML KYVFKAVLEE RADDMKFFAE RVDKDAVSRL
310 320 330 340 350
ERFIEADFAQ VDYTDAVTIL ENCGRKFENP VYWGVDLSSE HERYLAEEHF
360 370 380 390 400
KAPVVVKNYP KDIKAFYMRL NEDGKTVAAM DVLAPGIGEI IGGSQREERL
410 420 430 440 450
DVLDERMLEM GLNKEDYWWY RDLRRYGTVP HSGFGLGFER LIAYVTGVQN
460
VRDVIPFPRT PRNASF
Length:466
Mass (Da):52,570
Last modified:January 23, 2007 - v2
Checksum:i1E477CB5467B772F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti231 – 2311P → L in temperature-sensitive mutant HO202.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33145 Genomic DNA. Translation: AAA24666.1.
X68192 Genomic DNA. Translation: CAA48274.1.
U00096 Genomic DNA. Translation: AAC74016.1.
AP009048 Genomic DNA. Translation: BAA35682.1.
PIRiJS0396. SYECNT.
RefSeqiNP_415450.1. NC_000913.3.
WP_000117881.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74016; AAC74016; b0930.
BAA35682; BAA35682; BAA35682.
GeneIDi945555.
KEGGiecj:JW0913.
eco:b0930.
PATRICi32117077. VBIEscCol129921_0963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33145 Genomic DNA. Translation: AAA24666.1.
X68192 Genomic DNA. Translation: CAA48274.1.
U00096 Genomic DNA. Translation: AAC74016.1.
AP009048 Genomic DNA. Translation: BAA35682.1.
PIRiJS0396. SYECNT.
RefSeqiNP_415450.1. NC_000913.3.
WP_000117881.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A8M0.
SMRiP0A8M0. Positions 19-461.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-9179N.
IntActiP0A8M0. 3 interactions.
MINTiMINT-1319001.
STRINGi511145.b0930.

2D gel databases

SWISS-2DPAGEP0A8M0.

Proteomic databases

EPDiP0A8M0.
PaxDbiP0A8M0.
PRIDEiP0A8M0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74016; AAC74016; b0930.
BAA35682; BAA35682; BAA35682.
GeneIDi945555.
KEGGiecj:JW0913.
eco:b0930.
PATRICi32117077. VBIEscCol129921_0963.

Organism-specific databases

EchoBASEiEB0092.
EcoGeneiEG10094. asnS.

Phylogenomic databases

eggNOGiENOG4105C5S. Bacteria.
COG0017. LUCA.
HOGENOMiHOG000226033.
InParanoidiP0A8M0.
KOiK01893.
OMAiPEDMEFF.
PhylomeDBiP0A8M0.

Enzyme and pathway databases

BioCyciEcoCyc:ASNS-MONOMER.
ECOL316407:JW0913-MONOMER.
MetaCyc:ASNS-MONOMER.

Miscellaneous databases

PROiP0A8M0.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERiPTHR22594. PTHR22594. 2 hits.
PTHR22594:SF6. PTHR22594:SF6. 2 hits.
PfamiPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYN_ECOLI
AccessioniPrimary (citable) accession number: P0A8M0
Secondary accession number(s): P17242
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.