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Protein

Asparagine--tRNA ligase

Gene

asnS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).

GO - Molecular functioni

  • asparagine-tRNA ligase activity Source: EcoCyc
  • ATP binding Source: EcoCyc
  • nucleic acid binding Source: InterPro

GO - Biological processi

  • asparaginyl-tRNA aminoacylation Source: EcoCyc
  • tRNA aminoacylation for protein translation Source: EcoCyc

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ASNS-MONOMER
MetaCyc:ASNS-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligase (EC:6.1.1.22)
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name:
AsnRS
Gene namesi
Name:asnS
Synonyms:tss
Ordered Locus Names:b0930, JW0913
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG10094 asnS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi426Y → F: No effect. 1
Mutagenesisi426Y → S: 15-fold increase in Km for ATP. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001764062 – 466Asparagine--tRNA ligaseAdd BLAST465

Proteomic databases

EPDiP0A8M0
PaxDbiP0A8M0
PRIDEiP0A8M0

2D gel databases

SWISS-2DPAGEiP0A8M0

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259395, 8 interactors
DIPiDIP-9179N
IntActiP0A8M0, 3 interactors
STRINGi316385.ECDH10B_1000

Structurei

3D structure databases

ProteinModelPortaliP0A8M0
SMRiP0A8M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C5S Bacteria
COG0017 LUCA
HOGENOMiHOG000226033
InParanoidiP0A8M0
KOiK01893
OMAiWWYLDTR
PhylomeDBiP0A8M0

Family and domain databases

HAMAPiMF_00534 Asn_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR004364 aa-tRNA-synt_II
IPR006195 aa-tRNA-synth_II
IPR004522 Asn-tRNA-ligase
IPR002312 Asp/Asn-tRNA-synth_IIb
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
PfamiView protein in Pfam
PF00152 tRNA-synt_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
PRINTSiPR01042 TRNASYNTHASP
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00457 asnS, 1 hit
PROSITEiView protein in PROSITE
PS50862 AA_TRNA_LIGASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVVPVADVL QGRVAVDSEV TVRGWVRTRR DSKAGISFLA VYDGSCFDPV
60 70 80 90 100
QAVINNSLPN YNEDVLRLTT GCSVIVTGKV VASPGQGQQF EIQASKVEVA
110 120 130 140 150
GWVEDPDTYP MAAKRHSIEY LREVAHLRPR TNLIGAVARV RHTLAQALHR
160 170 180 190 200
FFNEQGFFWV STPLITASDT EGAGEMFRVS TLDLENLPRN DQGKVDFDKD
210 220 230 240 250
FFGKESFLTV SGQLNGETYA CALSKIYTFG PTFRAENSNT SRHLAEFWML
260 270 280 290 300
EPEVAFANLN DIAGLAEAML KYVFKAVLEE RADDMKFFAE RVDKDAVSRL
310 320 330 340 350
ERFIEADFAQ VDYTDAVTIL ENCGRKFENP VYWGVDLSSE HERYLAEEHF
360 370 380 390 400
KAPVVVKNYP KDIKAFYMRL NEDGKTVAAM DVLAPGIGEI IGGSQREERL
410 420 430 440 450
DVLDERMLEM GLNKEDYWWY RDLRRYGTVP HSGFGLGFER LIAYVTGVQN
460
VRDVIPFPRT PRNASF
Length:466
Mass (Da):52,570
Last modified:January 23, 2007 - v2
Checksum:i1E477CB5467B772F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti231P → L in temperature-sensitive mutant HO202. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33145 Genomic DNA Translation: AAA24666.1
X68192 Genomic DNA Translation: CAA48274.1
U00096 Genomic DNA Translation: AAC74016.1
AP009048 Genomic DNA Translation: BAA35682.1
PIRiJS0396 SYECNT
RefSeqiNP_415450.1, NC_000913.3
WP_000117881.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74016; AAC74016; b0930
BAA35682; BAA35682; BAA35682
GeneIDi945555
KEGGiecj:JW0913
eco:b0930
PATRICifig|1411691.4.peg.1345

Similar proteinsi

Entry informationi

Entry nameiSYN_ECOLI
AccessioniPrimary (citable) accession number: P0A8M0
Secondary accession number(s): P17242
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. SIMILARITY comments
    Index of protein domains and families

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