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P0A8J8

- RHLB_ECOLI

UniProt

P0A8J8 - RHLB_ECOLI

Protein

ATP-dependent RNA helicase RhlB

Gene

rhlB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    DEAD-box RNA helicase involved in RNA degradation. Has RNA-dependent ATPase activity and unwinds double-stranded RNA.3 PublicationsUniRule annotation

    Catalytic activityi

    ATP + H2O = ADP + phosphate.2 PublicationsUniRule annotation

    Enzyme regulationi

    ATPase activity is stimulated by interaction with RNase E.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi53 – 608ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. ATP-dependent helicase activity Source: EcoCyc
    3. ATP-dependent RNA helicase activity Source: UniProtKB-HAMAP
    4. protein binding Source: IntAct
    5. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. RNA catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10844-MONOMER.
    ECOL316407:JW3753-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent RNA helicase RhlBUniRule annotation (EC:3.6.4.13UniRule annotation)
    Gene namesi
    Name:rhlBUniRule annotation
    Synonyms:mmrA
    Ordered Locus Names:b3780, JW3753
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10844. rhlB.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation
    Note: Forms a cytoskeletal-like coiled structure that extends along the length of the cell. Formation of this structure does not require the presence of RNase E, MinD and/or MreB.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 421420ATP-dependent RNA helicase RhlBPRO_0000200769Add
    BLAST

    Proteomic databases

    PaxDbiP0A8J8.
    PRIDEiP0A8J8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A8J8.

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Binds to RNase E (rne) and PNPase (pnp). Forms multimers.4 PublicationsUniRule annotation

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    pnpP050556EBI-555806,EBI-548080
    rneP2151316EBI-555806,EBI-549958
    rneQ1ZS714EBI-555806,EBI-8525650From a different organism.

    Protein-protein interaction databases

    DIPiDIP-35644N.
    IntActiP0A8J8. 32 interactions.
    MINTiMINT-8049290.
    STRINGi511145.b3780.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A8J8.
    SMRiP0A8J8. Positions 9-379.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 219180Helicase ATP-bindingUniRule annotationAdd
    BLAST
    Domaini245 – 390146Helicase C-terminalUniRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 3729Q motifAdd
    BLAST
    Motifi165 – 1684DEAD box

    Sequence similaritiesi

    Belongs to the DEAD box helicase family. RhlB subfamily.UniRule annotation
    Contains 1 helicase ATP-binding domain.UniRule annotation
    Contains 1 helicase C-terminal domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    HOGENOMiHOG000268807.
    KOiK03732.
    OMAiPGRIIDY.
    OrthoDBiEOG6GBMBM.
    PhylomeDBiP0A8J8.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_00661. DEAD_helicase_RhlB.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR023554. RNA_helicase_ATP-dep_RhlB.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A8J8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA    50
    GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI 100
    HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK 150
    QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT 200
    LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL 250
    IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD 300
    EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA 350
    GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT 400
    RPRTGNGPRR TGAPRNRRRS G 421
    Length:421
    Mass (Da):47,126
    Last modified:January 23, 2007 - v2
    Checksum:i48E1ADD025CBFA1A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 721L → H in X56310. (PubMed:1931833)Curated
    Sequence conflicti340 – 3401Y → S in X56310. (PubMed:1931833)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56310 Genomic DNA. No translation available.
    M87049 Genomic DNA. Translation: AAA67581.1.
    M83316 Genomic DNA. Translation: AAB59048.1.
    U00096 Genomic DNA. Translation: AAC76785.1.
    AP009048 Genomic DNA. Translation: BAE77518.1.
    PIRiG65181.
    RefSeqiNP_418227.1. NC_000913.3.
    YP_491659.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76785; AAC76785; b3780.
    BAE77518; BAE77518; BAE77518.
    GeneIDi12934326.
    948290.
    KEGGiecj:Y75_p3395.
    eco:b3780.
    PATRICi32123051. VBIEscCol129921_3895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56310 Genomic DNA. No translation available.
    M87049 Genomic DNA. Translation: AAA67581.1 .
    M83316 Genomic DNA. Translation: AAB59048.1 .
    U00096 Genomic DNA. Translation: AAC76785.1 .
    AP009048 Genomic DNA. Translation: BAE77518.1 .
    PIRi G65181.
    RefSeqi NP_418227.1. NC_000913.3.
    YP_491659.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A8J8.
    SMRi P0A8J8. Positions 9-379.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35644N.
    IntActi P0A8J8. 32 interactions.
    MINTi MINT-8049290.
    STRINGi 511145.b3780.

    Proteomic databases

    PaxDbi P0A8J8.
    PRIDEi P0A8J8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76785 ; AAC76785 ; b3780 .
    BAE77518 ; BAE77518 ; BAE77518 .
    GeneIDi 12934326.
    948290.
    KEGGi ecj:Y75_p3395.
    eco:b3780.
    PATRICi 32123051. VBIEscCol129921_3895.

    Organism-specific databases

    EchoBASEi EB0837.
    EcoGenei EG10844. rhlB.

    Phylogenomic databases

    eggNOGi COG0513.
    HOGENOMi HOG000268807.
    KOi K03732.
    OMAi PGRIIDY.
    OrthoDBi EOG6GBMBM.
    PhylomeDBi P0A8J8.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10844-MONOMER.
    ECOL316407:JW3753-MONOMER.

    Miscellaneous databases

    PROi P0A8J8.

    Gene expression databases

    Genevestigatori P0A8J8.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_00661. DEAD_helicase_RhlB.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR023554. RNA_helicase_ATP-dep_RhlB.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote."
      Kalman M., Murphy H., Cashel M.
      New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
      Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
      Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "A DEAD-box RNA helicase in the Escherichia coli RNA degradosome."
      Py B., Higgins C.F., Krisch H.M., Carpousis A.J.
      Nature 381:169-172(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
    6. "Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome."
      Vanzo N.F., Li Y.S., Py B., Blum E., Higgins C.F., Raynal L.C., Krisch H.M., Carpousis A.J.
      Genes Dev. 12:2770-2781(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, INTERACTION WITH RNASE E, SUBUNIT.
    7. "Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase."
      Coburn G.A., Miao X., Briant D.J., Mackie G.A.
      Genes Dev. 13:2594-2603(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RNASE E, SUBUNIT.
    8. "DEAD box RhlB RNA helicase physically associates with exoribonuclease PNPase to degrade double-stranded RNA independent of the degradosome-assembling region of RNase E."
      Liou G.-G., Chang H.-Y., Lin C.-S., Lin-Chao S.
      J. Biol. Chem. 277:41157-41162(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PNPASE AND RNASE E.
    9. "RNaseE and RNA helicase B play central roles in the cytoskeletal organization of the RNA degradosome."
      Taghbalout A., Rothfield L.
      J. Biol. Chem. 283:13850-13855(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, SUBUNIT.

    Entry informationi

    Entry nameiRHLB_ECOLI
    AccessioniPrimary (citable) accession number: P0A8J8
    Secondary accession number(s): P24229, Q2M888
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3