P0A8J8 (RHLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 72.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: ATP-dependent RNA helicase RhlB EC=3.6.4.13 | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 421 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Can carry out ATP-dependent unwinding of double stranded RNA. Has a role in RNA decay. Involved in the RNA degradosome, a multi-enzyme complex important in RNA processing and messenger RNA degradation. Ref.5 |
| Catalytic activity | ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00661 |
| Subunit structure | Component of the degradosome complex. Binds to RNase E and PNPase. Forms multimers. Ref.6 |
| Sequence similarities | Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | ATP-binding Nucleotide-binding RNA-binding |
| Molecular function | Helicase Hydrolase |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | RNA catabolic process Inferred from direct assay PubMed 10521403. Source: EcoCyc |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: HAMAP ATP-dependent RNA helicase activityInferred from electronic annotation. Source: InterPro ATP-dependent helicase activityInferred from direct assay Ref.5. Source: EcoCyc RNA bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| rne | P21513 | 14 | EBI-555806,EBI-549958 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.5 | ||||||
| Chain | 2 – 421 | 420 | ATP-dependent RNA helicase RhlB HAMAP-Rule MF_00661 | PRO_0000200769 | |||||
Regions | |||||||||
| Domain | 40 – 219 | 180 | Helicase ATP-binding | ||||||
| Domain | 245 – 390 | 146 | Helicase C-terminal | ||||||
| Nucleotide binding | 53 – 60 | 8 | ATP By similarity | ||||||
| Motif | 9 – 37 | 29 | Q motif HAMAP-Rule MF_00661 | ||||||
| Motif | 165 – 168 | 4 | DEAD box HAMAP-Rule MF_00661 | ||||||
Experimental info | |||||||||
| Sequence conflict | 72 | 1 | L → H Ref.1 | ||||||
| Sequence conflict | 340 | 1 | Y → S Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote." Kalman M., Murphy H., Cashel M. New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "A DEAD-box RNA helicase in the Escherichia coli RNA degradosome." Py B., Higgins C.F., Krisch H.M., Carpousis A.J. Nature 381:169-172(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION IN DEGRADOSOME. |
| [6] | "DEAD box RhlB RNA helicase physically associates with exoribonuclease PNPase to degrade double-stranded RNA independent of the degradosome-assembling region of RNase E." Liou G.-G., Chang H.-Y., Lin C.-S., Lin-Chao S. J. Biol. Chem. 277:41157-41162(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PNPASE AND RNASE E. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X56310 Genomic DNA. No translation available. M87049 Genomic DNA. Translation: AAA67581.1. M83316 Genomic DNA. Translation: AAB59048.1. U00096 Genomic DNA. Translation: AAC76785.1. AP009048 Genomic DNA. Translation: BAE77518.1. |
| PIR | G65181. |
| RefSeq | NP_418227.1. NC_000913.2. YP_491659.1. NC_007779.1. |
3D structure databases | |
| ProteinModelPortal | P0A8J8. |
| SMR | P0A8J8. Positions 2-379. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-35644N. |
| IntAct | P0A8J8. 29 interactions. |
| STRING | 511145.b3780. |
Proteomic databases | |
| PaxDb | P0A8J8. |
| PRIDE | P0A8J8. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAC76785; AAC76785; b3780. BAE77518; BAE77518; BAE77518. |
| GeneID | 12934326. 948290. |
| KEGG | ecj:Y75_p3395. eco:b3780. |
| PATRIC | 32123051. VBIEscCol129921_3895. |
Organism-specific databases | |
| EchoBASE | EB0837. |
| EcoGene | EG10844. rhlB. |
Phylogenomic databases | |
| eggNOG | COG0513. |
| HOGENOM | HOG000268807. |
| KO | K03732. |
| OMA | HIDGIKY. |
| ProtClustDB | PRK04837. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:EG10844-MONOMER. ECOL316407:JW3753-MONOMER. |
Gene expression databases | |
| Genevestigator | P0A8J8. |
Family and domain databases | |
| HAMAP | MF_00661. Helicase_RhlB. |
| InterPro | IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR023554. RNA_helicase_ATP-dep_RhlB. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view] |
| Pfam | PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view] |
| SMART | SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view] |
| PROSITE | PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | RHLB_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0A8J8 Secondary accession number(s): P24229, Q2M888 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with
