P0A8J8 (RHLB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase RhlB
EC=3.6.4.13

Gene names

Name:	rhlB
Synonyms:	mmrA
Ordered Locus Names:	b3780, JW3753

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can carry out ATP-dependent unwinding of double stranded RNA. Has a role in RNA decay. Involved in the RNA degradosome, a multi-enzyme complex important in RNA processing and messenger RNA degradation. Ref.5
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00661
Subunit structure	Component of the degradosome complex. Binds to RNase E and PNPase. Forms multimers. Ref.6
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from direct assay PubMed 10521403. Source: EcoCyc
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP ATP-dependent RNA helicase activity Inferred from electronic annotation. Source: InterPro ATP-dependent helicase activity Inferred from direct assay Ref.5. Source: EcoCyc RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rne	P21513	14	EBI-555806,EBI-549958

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 421	420	ATP-dependent RNA helicase RhlB HAMAP-Rule MF_00661		PRO_0000200769
Regions
	Domain	40 – 219	180	Helicase ATP-binding
	Domain	245 – 390	146	Helicase C-terminal
	Nucleotide binding	53 – 60	8	ATP By similarity
	Motif	9 – 37	29	Q motif HAMAP-Rule MF_00661
	Motif	165 – 168	4	DEAD box HAMAP-Rule MF_00661
Experimental info
	Sequence conflict	72	1	L → H Ref.1
	Sequence conflict	340	1	Y → S Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A8J8 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 48E1ADD025CBFA1A	FASTA	421	47,126
	10 20 30 40 50 60 MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA GQAQTGTGKT 70 80 90 100 110 120 MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI HADAEPLAEA TGLKLGLAYG 130 140 150 160 170 180 GDGYDKQLKV LESGVDILIG TTGRLIDYAK QNHINLGAIQ VVVLDEADRM YDLGFIKDIR 190 200 210 220 230 240 WLFRRMPPAN QRLNMLFSAT LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN 250 260 270 280 290 300 EEKMRLLQTL IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD 310 320 330 340 350 360 EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA GASGHSISLA 370 380 390 400 410 420 CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT RPRTGNGPRR TGAPRNRRRS G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote." Kalman M., Murphy H., Cashel M. New Biol. 3:886-895(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A DEAD-box RNA helicase in the Escherichia coli RNA degradosome." Py B., Higgins C.F., Krisch H.M., Carpousis A.J. Nature 381:169-172(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16, FUNCTION IN DEGRADOSOME.
[6]	"DEAD box RhlB RNA helicase physically associates with exoribonuclease PNPase to degrade double-stranded RNA independent of the degradosome-assembling region of RNase E." Liou G.-G., Chang H.-Y., Lin C.-S., Lin-Chao S. J. Biol. Chem. 277:41157-41162(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PNPASE AND RNASE E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56310 Genomic DNA. No translation available. M87049 Genomic DNA. Translation: AAA67581.1. M83316 Genomic DNA. Translation: AAB59048.1. U00096 Genomic DNA. Translation: AAC76785.1. AP009048 Genomic DNA. Translation: BAE77518.1.
PIR	G65181.
RefSeq	NP_418227.1. NC_000913.2. YP_491659.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A8J8.
SMR	P0A8J8. Positions 2-379.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35644N.
IntAct	P0A8J8. 29 interactions.
STRING	511145.b3780.
Proteomic databases
PaxDb	P0A8J8.
PRIDE	P0A8J8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76785; AAC76785; b3780. BAE77518; BAE77518; BAE77518.
GeneID	12934326. 948290.
KEGG	ecj:Y75_p3395. eco:b3780.
PATRIC	32123051. VBIEscCol129921_3895.
Organism-specific databases
EchoBASE	EB0837.
EcoGene	EG10844. rhlB.
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000268807.
KO	K03732.
OMA	HIDGIKY.
ProtClustDB	PRK04837.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10844-MONOMER. ECOL316407:JW3753-MONOMER.
Gene expression databases
Genevestigator	P0A8J8.
Family and domain databases
HAMAP	MF_00661. Helicase_RhlB.
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR023554. RNA_helicase_ATP-dep_RhlB. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHLB_ECOLI

Accession

Primary (citable) accession number: P0A8J8
Secondary accession number(s): P24229, Q2M888

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program