Reviewed, UniProtKB/Swiss-Prot P0A8J2 (DNAT_ECOLI)

Names and origin

Protein names

Recommended name:
    Primosomal protein 1
Alternative name(s):
    Primosomal protein I

Gene names

Name:	dnaT
Ordered Locus Names:	b4362, JW4326

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with dnaB protein and other prepriming proteins dnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'. HAMAP MF_01061
Sequence similarities	Belongs to the dnaT family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: HAMAP
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yheB	Q9F505	1	EBI-549621,EBI-549635	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 179	178	Primosomal protein 1 HAMAP MF_01061		PRO_0000199869
Experimental info
	Sequence conflict	28	1	E → F AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A8J2-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8DE048A3B2A6235B	FASTA	179	19,455
	10 20 30 40 50 60 MSSRVLTPDV VGIDALVHDH QTVLAKAEGG VVAVFANNAP AFYAVTPARL AELLALEEKL 70 80 90 100 110 120 ARPGSDVALD DQLYQEPQAA PVAVPMGKFA MYPDWQPDAD FIRLAALWGV ALREPVTTEE 130 140 150 160 170 LASFIAYWQA EGKVFHHVQW QQKLARSLQI GRASNGGLPK RDVNTVSEPD SQIPPGFRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication." Masai H., Bond M.W., Arai K. Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed: 3006041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-41.
[2]	"Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome." Masai H., Arai K. J. Biol. Chem. 263:15083-15093(1988) [PubMed: 2844800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein." Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K. J. Biol. Chem. 262:10475-10480(1987) [PubMed: 3301836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-179.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04030 Genomic DNA. Translation: AAA23699.1. U14003 Genomic DNA. Translation: AAA97261.1. U00096 Genomic DNA. Translation: AAC77318.1. AP009048 Genomic DNA. Translation: BAE78352.1.
PIR	RMECI. S56589.
RefSeq	AP_004851.1. NP_418782.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A8J2. 2 interactions.
STRING	P0A8J2.
2-D gel databases
ECO2DBASE	C019.5. 6TH EDITION.
Genome annotation databases
GeneID	948813.
GenomeReviews	Gene locus JW4326 in contig AP009048_GR. Gene locus b4362 in contig U00096_GR.
KEGG	ecj:JW4326. eco:b4362.
Organism-specific databases
EchoBASE	EB0240.
EcoGene	EG10244. dnaT.
CMR	Search...
Phylogenomic databases
eggNOG	NOG148646.
HOGENOM	HBG416722.
OMA	FVAYWQA.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10244-MONOMER. ECOL168927:B4362-MONOMER.
Gene expression databases
Genevestigator	P0A8J2.
Family and domain databases
HAMAP	MF_01061. DnaT. [Tree]
InterPro	IPR020917. Primosomal_protein_1. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAT_ECOLI

Accession

Primary (citable) accession number: P0A8J2
Secondary accession number(s): P07904, Q2M5V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)