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Protein

Primosomal protein 1

Gene

dnaT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with DnaB protein and other prepriming proteins DnaC, N, N', N'' a prepriming protein complex on the specific site of the template DNA recognized by protein N'.

GO - Molecular functioni

  • identical protein binding Source: IntAct

GO - Biological processi

  • DNA-dependent DNA replication Source: EcoCyc
  • DNA replication, synthesis of RNA primer Source: UniProtKB-HAMAP
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG10244-MONOMER.
ECOL316407:JW4326-MONOMER.
MetaCyc:EG10244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Primosomal protein 1
Alternative name(s):
Primosomal protein I
Gene namesi
Name:dnaT
Ordered Locus Names:b4362, JW4326
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10244. dnaT.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 179178Primosomal protein 1PRO_0000199869Add
BLAST

Proteomic databases

PaxDbiP0A8J2.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-549621,EBI-549621
priBP070134EBI-549621,EBI-1125223

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4262774. 122 interactions.
DIPiDIP-47957N.
IntActiP0A8J2. 5 interactions.
MINTiMINT-1256745.
STRINGi511145.b4362.

Structurei

Secondary structure

1
179
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi87 – 904Combined sources
Helixi101 – 1077Combined sources
Helixi118 – 13114Combined sources
Beta strandi134 – 1363Combined sources
Helixi137 – 15115Combined sources
Turni152 – 1554Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RU8NMR-A89-179[»]
4OU6X-ray1.96A/B/C/D/E84-159[»]
4OU7X-ray2.83A/B/C/D/E84-154[»]
ProteinModelPortaliP0A8J2.
SMRiP0A8J2. Positions 89-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaT family.Curated

Phylogenomic databases

eggNOGiENOG4108PM0. Bacteria.
ENOG410ZNDQ. LUCA.
HOGENOMiHOG000267810.
KOiK02317.
OMAiASFVAYW.
OrthoDBiEOG6HTNXW.

Family and domain databases

HAMAPiMF_01061. DnaT.
InterProiIPR020917. Primosomal_protein_1.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRVLTPDV VGIDALVHDH QTVLAKAEGG VVAVFANNAP AFYAVTPARL
60 70 80 90 100
AELLALEEKL ARPGSDVALD DQLYQEPQAA PVAVPMGKFA MYPDWQPDAD
110 120 130 140 150
FIRLAALWGV ALREPVTTEE LASFIAYWQA EGKVFHHVQW QQKLARSLQI
160 170
GRASNGGLPK RDVNTVSEPD SQIPPGFRG
Length:179
Mass (Da):19,455
Last modified:January 23, 2007 - v2
Checksum:i8DE048A3B2A6235B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281E → F AA sequence (PubMed:3006041).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04030 Genomic DNA. Translation: AAA23699.1.
U14003 Genomic DNA. Translation: AAA97261.1.
U00096 Genomic DNA. Translation: AAC77318.1.
AP009048 Genomic DNA. Translation: BAE78352.1.
PIRiS56589. RMECI.
RefSeqiNP_418782.1. NC_000913.3.
WP_000098818.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77318; AAC77318; b4362.
BAE78352; BAE78352; BAE78352.
GeneIDi948813.
KEGGiecj:JW4326.
eco:b4362.
PATRICi32124332. VBIEscCol129921_4509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04030 Genomic DNA. Translation: AAA23699.1.
U14003 Genomic DNA. Translation: AAA97261.1.
U00096 Genomic DNA. Translation: AAC77318.1.
AP009048 Genomic DNA. Translation: BAE78352.1.
PIRiS56589. RMECI.
RefSeqiNP_418782.1. NC_000913.3.
WP_000098818.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RU8NMR-A89-179[»]
4OU6X-ray1.96A/B/C/D/E84-159[»]
4OU7X-ray2.83A/B/C/D/E84-154[»]
ProteinModelPortaliP0A8J2.
SMRiP0A8J2. Positions 89-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262774. 122 interactions.
DIPiDIP-47957N.
IntActiP0A8J2. 5 interactions.
MINTiMINT-1256745.
STRINGi511145.b4362.

Proteomic databases

PaxDbiP0A8J2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77318; AAC77318; b4362.
BAE78352; BAE78352; BAE78352.
GeneIDi948813.
KEGGiecj:JW4326.
eco:b4362.
PATRICi32124332. VBIEscCol129921_4509.

Organism-specific databases

EchoBASEiEB0240.
EcoGeneiEG10244. dnaT.

Phylogenomic databases

eggNOGiENOG4108PM0. Bacteria.
ENOG410ZNDQ. LUCA.
HOGENOMiHOG000267810.
KOiK02317.
OMAiASFVAYW.
OrthoDBiEOG6HTNXW.

Enzyme and pathway databases

BioCyciEcoCyc:EG10244-MONOMER.
ECOL316407:JW4326-MONOMER.
MetaCyc:EG10244-MONOMER.

Miscellaneous databases

PROiP0A8J2.

Family and domain databases

HAMAPiMF_01061. DnaT.
InterProiIPR020917. Primosomal_protein_1.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the Escherichia coli gene for primosomal protein I: the relationship to dnaT, essential for chromosomal DNA replication."
    Masai H., Bond M.W., Arai K.
    Proc. Natl. Acad. Sci. U.S.A. 83:1256-1260(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-41.
  2. "Operon structure of dnaT and dnaC genes essential for normal and stable DNA replication of Escherichia coli chromosome."
    Masai H., Arai K.
    J. Biol. Chem. 263:15083-15093(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein."
    Nakayama N., Bond M.W., Miyajima A., Kobori J., Arai K.
    J. Biol. Chem. 262:10475-10480(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 108-179.
  7. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiDNAT_ECOLI
AccessioniPrimary (citable) accession number: P0A8J2
Secondary accession number(s): P07904, Q2M5V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.