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P0A8I5

- TRMB_ECOLI

UniProt

P0A8I5 - TRMB_ECOLI

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionineBy similarity
Binding sitei94 – 941S-adenosyl-L-methionineBy similarity
Binding sitei121 – 1211S-adenosyl-L-methionineBy similarity
Active sitei144 – 1441By similarity
Binding sitei144 – 1441S-adenosyl-L-methionineBy similarity
Binding sitei148 – 1481SubstrateBy similarity
Binding sitei180 – 1801SubstrateBy similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: EcoCyc
  2. tRNA methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11779-MONOMER.
ECOL316407:JW2927-MONOMER.
MetaCyc:EG11779-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Synonyms:trmI, yggH
Ordered Locus Names:b2960, JW2927
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11779. trmI.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi26 – 261R → A: Reduces catalytic activity over 10-fold. 1 Publication
Mutagenesisi144 – 1441D → A: Loss of activity. 1 Publication
Mutagenesisi150 – 1501R → A: Reduces catalytic activity about 3-fold. 1 Publication
Mutagenesisi151 – 1511H → A: Reduces catalytic activity over 10-fold. 1 Publication
Mutagenesisi152 – 1521N → A: No effect. 1 Publication
Mutagenesisi154 – 1541R → A: Loss of activity. 1 Publication
Mutagenesisi155 – 1551R → A: Loss of activity. 1 Publication
Mutagenesisi180 – 1801D → A: Reduces catalytic activity over 10-fold. 1 Publication
Mutagenesisi217 – 2171T → A: Reduces catalytic activity over 10-fold. 1 Publication
Mutagenesisi220 – 2201E → A: Reduces catalytic activity 10-fold. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferasePRO_0000171326Add
BLAST

Proteomic databases

PaxDbiP0A8I5.
PRIDEiP0A8I5.

Expressioni

Gene expression databases

GenevestigatoriP0A8I5.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

DIPiDIP-36015N.
IntActiP0A8I5. 45 interactions.
STRINGi511145.b2960.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 459
Helixi56 – 605
Beta strandi66 – 716
Helixi76 – 849
Beta strandi88 – 936
Helixi97 – 10913
Beta strandi113 – 1186
Helixi122 – 1298
Beta strandi135 – 1428
Helixi149 – 1546
Helixi159 – 16810
Beta strandi169 – 18012
Helixi182 – 19312
Beta strandi198 – 2003
Beta strandi231 – 2377

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DXXX-ray2.05A33-239[»]
3DXYX-ray1.50A33-239[»]
3DXZX-ray1.58A33-239[»]
ProteinModelPortaliP0A8I5.
SMRiP0A8I5. Positions 36-239.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8I5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1556Interaction with RNASequence Analysis
Regioni217 – 2204Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
InParanoidiP0A8I5.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.
PhylomeDBiP0A8I5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8I5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQEHALEN YWPVMGVEFS
60 70 80 90 100
EDMLDFPALF GREAPVTLEI GFGMGASLVA MAKDRPEQDF LGIEVHSPGV
110 120 130 140 150
GACLASAHEE GLSNLRVMCH DAVEVLHKMI PDNSLRMVQL FFPDPWHKAR
160 170 180 190 200
HNKRRIVQVP FAELVKSKLQ LGGVFHMATD WEPYAEHMLE VMSSIDGYKN
210 220 230
LSESNDYVPR PASRPVTKFE QRGHRLGHGV WDLMFERVK
Length:239
Mass (Da):27,307
Last modified:June 7, 2005 - v1
Checksum:iB4C8AF9A1EF85FEB
GO

Sequence cautioni

The sequence AAA72956.1 differs from that shown. Reason: Frameshift at position 29.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59471 Genomic DNA. Translation: AAA72956.1. Frameshift.
U28377 Genomic DNA. Translation: AAA69127.1.
U00096 Genomic DNA. Translation: AAC75997.1.
AP009048 Genomic DNA. Translation: BAE77023.1.
PIRiG65081.
RefSeqiNP_417435.1. NC_000913.3.
YP_491159.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75997; AAC75997; b2960.
BAE77023; BAE77023; BAE77023.
GeneIDi12933364.
947448.
KEGGiecj:Y75_p2890.
eco:b2960.
PATRICi32121334. VBIEscCol129921_3055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59471 Genomic DNA. Translation: AAA72956.1 . Frameshift.
U28377 Genomic DNA. Translation: AAA69127.1 .
U00096 Genomic DNA. Translation: AAC75997.1 .
AP009048 Genomic DNA. Translation: BAE77023.1 .
PIRi G65081.
RefSeqi NP_417435.1. NC_000913.3.
YP_491159.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DXX X-ray 2.05 A 33-239 [» ]
3DXY X-ray 1.50 A 33-239 [» ]
3DXZ X-ray 1.58 A 33-239 [» ]
ProteinModelPortali P0A8I5.
SMRi P0A8I5. Positions 36-239.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-36015N.
IntActi P0A8I5. 45 interactions.
STRINGi 511145.b2960.

Proteomic databases

PaxDbi P0A8I5.
PRIDEi P0A8I5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75997 ; AAC75997 ; b2960 .
BAE77023 ; BAE77023 ; BAE77023 .
GeneIDi 12933364.
947448.
KEGGi ecj:Y75_p2890.
eco:b2960.
PATRICi 32121334. VBIEscCol129921_3055.

Organism-specific databases

EchoBASEi EB1727.
EcoGenei EG11779. trmI.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
InParanoidi P0A8I5.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.
PhylomeDBi P0A8I5.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci EcoCyc:EG11779-MONOMER.
ECOL316407:JW2927-MONOMER.
MetaCyc:EG11779-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A8I5.
PROi P0A8I5.

Gene expression databases

Genevestigatori P0A8I5.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY."
    Tsai-Wu J.-J., Radicella J.P., Lu A.-L.
    J. Bacteriol. 173:1902-1910(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase."
    De Bie L.G.S., Roovers M., Oudjama Y., Wattiez R., Tricot C., Stalon V., Droogmans L., Bujnicki J.M.
    J. Bacteriol. 185:3238-3243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBUNIT.
    Strain: K12 / XL1-Blue.
  5. "Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis."
    Purta E., van Vliet F., Tricot C., De Bie L.G., Feder M., Skowronek K., Droogmans L., Bujnicki J.M.
    Proteins 59:482-488(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-26; ASP-144; ARG-150; HIS-151; ASN-152; ARG-154; ARG-155; ASP-180; THR-217 AND GLU-220.

Entry informationi

Entry nameiTRMB_ECOLI
AccessioniPrimary (citable) accession number: P0A8I5
Secondary accession number(s): P32049, P58089, Q2M9N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3