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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation1 Publication

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei94S-adenosyl-L-methionineUniRule annotation1
Binding sitei121S-adenosyl-L-methionineUniRule annotation1
Active sitei144By similarity1
Binding sitei144S-adenosyl-L-methionineUniRule annotation1
Binding sitei148SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1

GO - Molecular functioni

  • tRNA (guanine-N7-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • RNA (guanine-N7)-methylation Source: EcoCyc
  • tRNA methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11779-MONOMER.
ECOL316407:JW2927-MONOMER.
MetaCyc:EG11779-MONOMER.
BRENDAi2.1.1.33. 2026.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation1 Publication)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferase1 PublicationUniRule annotation
Gene namesi
Name:trmB
Synonyms:trmI, yggH
Ordered Locus Names:b2960, JW2927
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11779. trmI.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi26R → A: Reduces catalytic activity over 10-fold. 1 Publication1
Mutagenesisi144D → A: Loss of activity. 1 Publication1
Mutagenesisi150R → A: Reduces catalytic activity about 3-fold. 1 Publication1
Mutagenesisi151H → A: Reduces catalytic activity over 10-fold. 1 Publication1
Mutagenesisi152N → A: No effect. 1 Publication1
Mutagenesisi154R → A: Loss of activity. 1 Publication1
Mutagenesisi155R → A: Loss of activity. 1 Publication1
Mutagenesisi180D → A: Reduces catalytic activity over 10-fold. 1 Publication1
Mutagenesisi217T → A: Reduces catalytic activity over 10-fold. 1 Publication1
Mutagenesisi220E → A: Reduces catalytic activity 10-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713261 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Proteomic databases

PaxDbiP0A8I5.
PRIDEiP0A8I5.

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4262358. 9 interactors.
DIPiDIP-36015N.
IntActiP0A8I5. 45 interactors.
STRINGi511145.b2960.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 45Combined sources9
Helixi56 – 60Combined sources5
Beta strandi66 – 71Combined sources6
Helixi76 – 84Combined sources9
Beta strandi88 – 93Combined sources6
Helixi97 – 109Combined sources13
Beta strandi113 – 118Combined sources6
Helixi122 – 129Combined sources8
Beta strandi135 – 142Combined sources8
Helixi149 – 154Combined sources6
Helixi159 – 168Combined sources10
Beta strandi169 – 180Combined sources12
Helixi182 – 193Combined sources12
Beta strandi198 – 200Combined sources3
Beta strandi231 – 237Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXXX-ray2.05A33-239[»]
3DXYX-ray1.50A33-239[»]
3DXZX-ray1.58A33-239[»]
ProteinModelPortaliP0A8I5.
SMRiP0A8I5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8I5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 155Interaction with RNAUniRule annotation6
Regioni217 – 220Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
InParanoidiP0A8I5.
KOiK03439.
OMAiGNLRVMC.
PhylomeDBiP0A8I5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8I5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQEHALEN YWPVMGVEFS
60 70 80 90 100
EDMLDFPALF GREAPVTLEI GFGMGASLVA MAKDRPEQDF LGIEVHSPGV
110 120 130 140 150
GACLASAHEE GLSNLRVMCH DAVEVLHKMI PDNSLRMVQL FFPDPWHKAR
160 170 180 190 200
HNKRRIVQVP FAELVKSKLQ LGGVFHMATD WEPYAEHMLE VMSSIDGYKN
210 220 230
LSESNDYVPR PASRPVTKFE QRGHRLGHGV WDLMFERVK
Length:239
Mass (Da):27,307
Last modified:June 7, 2005 - v1
Checksum:iB4C8AF9A1EF85FEB
GO

Sequence cautioni

The sequence AAA72956 differs from that shown. Reason: Frameshift at position 29.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59471 Genomic DNA. Translation: AAA72956.1. Frameshift.
U28377 Genomic DNA. Translation: AAA69127.1.
U00096 Genomic DNA. Translation: AAC75997.1.
AP009048 Genomic DNA. Translation: BAE77023.1.
PIRiG65081.
RefSeqiNP_417435.1. NC_000913.3.
WP_000786911.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75997; AAC75997; b2960.
BAE77023; BAE77023; BAE77023.
GeneIDi947448.
KEGGiecj:JW2927.
eco:b2960.
PATRICi32121334. VBIEscCol129921_3055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59471 Genomic DNA. Translation: AAA72956.1. Frameshift.
U28377 Genomic DNA. Translation: AAA69127.1.
U00096 Genomic DNA. Translation: AAC75997.1.
AP009048 Genomic DNA. Translation: BAE77023.1.
PIRiG65081.
RefSeqiNP_417435.1. NC_000913.3.
WP_000786911.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXXX-ray2.05A33-239[»]
3DXYX-ray1.50A33-239[»]
3DXZX-ray1.58A33-239[»]
ProteinModelPortaliP0A8I5.
SMRiP0A8I5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262358. 9 interactors.
DIPiDIP-36015N.
IntActiP0A8I5. 45 interactors.
STRINGi511145.b2960.

Proteomic databases

PaxDbiP0A8I5.
PRIDEiP0A8I5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75997; AAC75997; b2960.
BAE77023; BAE77023; BAE77023.
GeneIDi947448.
KEGGiecj:JW2927.
eco:b2960.
PATRICi32121334. VBIEscCol129921_3055.

Organism-specific databases

EchoBASEiEB1727.
EcoGeneiEG11779. trmI.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
InParanoidiP0A8I5.
KOiK03439.
OMAiGNLRVMC.
PhylomeDBiP0A8I5.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciEcoCyc:EG11779-MONOMER.
ECOL316407:JW2927-MONOMER.
MetaCyc:EG11779-MONOMER.
BRENDAi2.1.1.33. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A8I5.
PROiP0A8I5.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_ECOLI
AccessioniPrimary (citable) accession number: P0A8I5
Secondary accession number(s): P32049, P58089, Q2M9N3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.