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P0A8I5

- TRMB_ECOLI

UniProt

P0A8I5 - TRMB_ECOLI

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691S-adenosyl-L-methionineBy similarity
    Binding sitei94 – 941S-adenosyl-L-methionineBy similarity
    Binding sitei121 – 1211S-adenosyl-L-methionineBy similarity
    Active sitei144 – 1441By similarity
    Binding sitei144 – 1441S-adenosyl-L-methionineBy similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Binding sitei180 – 1801SubstrateBy similarity

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. RNA (guanine-N7)-methylation Source: EcoCyc
    2. tRNA methylation Source: EcoCyc

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11779-MONOMER.
    ECOL316407:JW2927-MONOMER.
    MetaCyc:EG11779-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Synonyms:trmI, yggH
    Ordered Locus Names:b2960, JW2927
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11779. trmI.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi26 – 261R → A: Reduces catalytic activity over 10-fold. 1 Publication
    Mutagenesisi144 – 1441D → A: Loss of activity. 1 Publication
    Mutagenesisi150 – 1501R → A: Reduces catalytic activity about 3-fold. 1 Publication
    Mutagenesisi151 – 1511H → A: Reduces catalytic activity over 10-fold. 1 Publication
    Mutagenesisi152 – 1521N → A: No effect. 1 Publication
    Mutagenesisi154 – 1541R → A: Loss of activity. 1 Publication
    Mutagenesisi155 – 1551R → A: Loss of activity. 1 Publication
    Mutagenesisi180 – 1801D → A: Reduces catalytic activity over 10-fold. 1 Publication
    Mutagenesisi217 – 2171T → A: Reduces catalytic activity over 10-fold. 1 Publication
    Mutagenesisi220 – 2201E → A: Reduces catalytic activity 10-fold. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferasePRO_0000171326Add
    BLAST

    Proteomic databases

    PaxDbiP0A8I5.
    PRIDEiP0A8I5.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A8I5.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-36015N.
    IntActiP0A8I5. 45 interactions.
    STRINGi511145.b2960.

    Structurei

    Secondary structure

    1
    239
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi37 – 459
    Helixi56 – 605
    Beta strandi66 – 716
    Helixi76 – 849
    Beta strandi88 – 936
    Helixi97 – 10913
    Beta strandi113 – 1186
    Helixi122 – 1298
    Beta strandi135 – 1428
    Helixi149 – 1546
    Helixi159 – 16810
    Beta strandi169 – 18012
    Helixi182 – 19312
    Beta strandi198 – 2003
    Beta strandi231 – 2377

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3DXXX-ray2.05A33-239[»]
    3DXYX-ray1.50A33-239[»]
    3DXZX-ray1.58A33-239[»]
    ProteinModelPortaliP0A8I5.
    SMRiP0A8I5. Positions 36-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A8I5.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 1556Interaction with RNASequence Analysis
    Regioni217 – 2204Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.
    PhylomeDBiP0A8I5.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A8I5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQEHALEN YWPVMGVEFS    50
    EDMLDFPALF GREAPVTLEI GFGMGASLVA MAKDRPEQDF LGIEVHSPGV 100
    GACLASAHEE GLSNLRVMCH DAVEVLHKMI PDNSLRMVQL FFPDPWHKAR 150
    HNKRRIVQVP FAELVKSKLQ LGGVFHMATD WEPYAEHMLE VMSSIDGYKN 200
    LSESNDYVPR PASRPVTKFE QRGHRLGHGV WDLMFERVK 239
    Length:239
    Mass (Da):27,307
    Last modified:June 7, 2005 - v1
    Checksum:iB4C8AF9A1EF85FEB
    GO

    Sequence cautioni

    The sequence AAA72956.1 differs from that shown. Reason: Frameshift at position 29.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59471 Genomic DNA. Translation: AAA72956.1. Frameshift.
    U28377 Genomic DNA. Translation: AAA69127.1.
    U00096 Genomic DNA. Translation: AAC75997.1.
    AP009048 Genomic DNA. Translation: BAE77023.1.
    PIRiG65081.
    RefSeqiNP_417435.1. NC_000913.3.
    YP_491159.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75997; AAC75997; b2960.
    BAE77023; BAE77023; BAE77023.
    GeneIDi12933364.
    947448.
    KEGGiecj:Y75_p2890.
    eco:b2960.
    PATRICi32121334. VBIEscCol129921_3055.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59471 Genomic DNA. Translation: AAA72956.1 . Frameshift.
    U28377 Genomic DNA. Translation: AAA69127.1 .
    U00096 Genomic DNA. Translation: AAC75997.1 .
    AP009048 Genomic DNA. Translation: BAE77023.1 .
    PIRi G65081.
    RefSeqi NP_417435.1. NC_000913.3.
    YP_491159.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3DXX X-ray 2.05 A 33-239 [» ]
    3DXY X-ray 1.50 A 33-239 [» ]
    3DXZ X-ray 1.58 A 33-239 [» ]
    ProteinModelPortali P0A8I5.
    SMRi P0A8I5. Positions 36-239.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36015N.
    IntActi P0A8I5. 45 interactions.
    STRINGi 511145.b2960.

    Proteomic databases

    PaxDbi P0A8I5.
    PRIDEi P0A8I5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75997 ; AAC75997 ; b2960 .
    BAE77023 ; BAE77023 ; BAE77023 .
    GeneIDi 12933364.
    947448.
    KEGGi ecj:Y75_p2890.
    eco:b2960.
    PATRICi 32121334. VBIEscCol129921_3055.

    Organism-specific databases

    EchoBASEi EB1727.
    EcoGenei EG11779. trmI.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.
    PhylomeDBi P0A8I5.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci EcoCyc:EG11779-MONOMER.
    ECOL316407:JW2927-MONOMER.
    MetaCyc:EG11779-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A8I5.
    PROi P0A8I5.

    Gene expression databases

    Genevestigatori P0A8I5.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY."
      Tsai-Wu J.-J., Radicella J.P., Lu A.-L.
      J. Bacteriol. 173:1902-1910(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase."
      De Bie L.G.S., Roovers M., Oudjama Y., Wattiez R., Tricot C., Stalon V., Droogmans L., Bujnicki J.M.
      J. Bacteriol. 185:3238-3243(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, SUBUNIT.
      Strain: K12 / XL1-Blue.
    5. "Sequence-structure-function relationships of a tRNA (m7G46) methyltransferase studied by homology modeling and site-directed mutagenesis."
      Purta E., van Vliet F., Tricot C., De Bie L.G., Feder M., Skowronek K., Droogmans L., Bujnicki J.M.
      Proteins 59:482-488(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-26; ASP-144; ARG-150; HIS-151; ASN-152; ARG-154; ARG-155; ASP-180; THR-217 AND GLU-220.

    Entry informationi

    Entry nameiTRMB_ECOLI
    AccessioniPrimary (citable) accession number: P0A8I5
    Secondary accession number(s): P32049, P58089, Q2M9N3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3