Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative pre-16S rRNA nuclease

Gene

yqgF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the processing of the 5'-end of pre-16S rRNA during 70S ribosome maturation (processing does not occur on total cellular RNA off the ribosome); may be a nuclease (PubMed:25545592). A temperature-sensitive yqgF mutant no longer grows when Rho or NusA are overproduced, and has reduced transcription of genes encoded downstream of Rho terminators; transcription increases again in the presence of the Rho inhibitor bicylomycin (PubMed:22353788).2 Publications

GO - Molecular functioni

GO - Biological processi

  • ribosome biogenesis Source: UniProtKB
  • rRNA 5'-end processing Source: UniProtKB
  • transcription antitermination Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

Ribosome biogenesis

Enzyme and pathway databases

BioCyciEcoCyc:G7525-MONOMER.
ECOL316407:JW2916-MONOMER.
MetaCyc:G7525-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pre-16S rRNA nucleaseUniRule annotationCurated (EC:3.1.-.-UniRule annotation)
Gene namesi
Name:yqgFUniRule annotation
Synonyms:ruvXCurated
Ordered Locus Names:b2949, JW2916
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13290. yqgF.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Essential, it cannot be disrupted.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9D → A: Does not process pre-16S rRNA in 70S ribosomes, binds to pre-16S rRNA-containing ribosomes. 1 Publication1
Mutagenesisi96E → A: Does not process pre-16S rRNA in 70S ribosomes. 1 Publication1
Mutagenesisi100T → I: Does not process pre-16S rRNA in 70S ribosomes. 1 Publication1
Mutagenesisi103A → I: Allows cell growth, i.e. not toxic. 1 Publication1
Mutagenesisi105S → I: Allows cell growth, i.e. not toxic. 1 Publication1
Mutagenesisi108 – 115Missing : Does not process pre-16S rRNA in 70S ribosomes. 1 Publication8
Mutagenesisi122D → A: Does not process pre-16S rRNA in 70S ribosomes, binds quite strongly to pre-16S rRNA-containing ribosomes. 1 Publication1
Mutagenesisi125S → A: Allows cell growth, i.e. not toxic. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001720581 – 138Putative pre-16S rRNA nucleaseAdd BLAST138

Proteomic databases

PaxDbiP0A8I1.
PRIDEiP0A8I1.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259241. 196 interactors.
IntActiP0A8I1. 9 interactors.
MINTiMINT-1317755.
STRINGi511145.b2949.

Structurei

Secondary structure

1138
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Beta strandi12 – 21Combined sources10
Turni22 – 25Combined sources4
Beta strandi26 – 36Combined sources11
Helixi42 – 52Combined sources11
Beta strandi55 – 63Combined sources9
Beta strandi65 – 68Combined sources4
Helixi71 – 87Combined sources17
Beta strandi91 – 97Combined sources7
Helixi118 – 121Combined sources4
Helixi124 – 135Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NMNX-ray2.30A/B1-138[»]
1NU0X-ray1.60A/B1-138[»]
1OVQNMR-A1-138[»]
ProteinModelPortaliP0A8I1.
SMRiP0A8I1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8I1.

Family & Domainsi

Sequence similaritiesi

Belongs to the YqgF nuclease family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107Y66. Bacteria.
COG0816. LUCA.
HOGENOMiHOG000016954.
InParanoidiP0A8I1.
KOiK07447.
OMAiPMGWTAQ.
PhylomeDBiP0A8I1.

Family and domain databases

Gene3Di3.30.420.140. 1 hit.
HAMAPiMF_00651. Nuclease_YqgF. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR005227. YqgF.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PfamiPF03652. RuvX. 1 hit.
[Graphical view]
SMARTiSM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00250. RNAse_H_YqgF. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8I1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTLLAFDF GTKSIGVAVG QRITGTARPL PAIKAQDGTP DWNIIERLLK
60 70 80 90 100
EWQPDEIIVG LPLNMDGTEQ PLTARARKFA NRIHGRFGVE VKLHDERLST
110 120 130
VEARSGLFEQ GGYRALNKGK VDSASAVIIL ESYFEQGY
Length:138
Mass (Da):15,186
Last modified:June 7, 2005 - v1
Checksum:i89650665AA5C1E50
GO

Sequence cautioni

The sequence AAA69116 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69116.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75986.1.
AP009048 Genomic DNA. Translation: BAE77012.1.
PIRiD65080.
RefSeqiNP_417424.1. NC_000913.3.
WP_000017106.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75986; AAC75986; b2949.
BAE77012; BAE77012; BAE77012.
GeneIDi947439.
KEGGiecj:JW2916.
eco:b2949.
PATRICi32121308. VBIEscCol129921_3042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69116.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75986.1.
AP009048 Genomic DNA. Translation: BAE77012.1.
PIRiD65080.
RefSeqiNP_417424.1. NC_000913.3.
WP_000017106.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NMNX-ray2.30A/B1-138[»]
1NU0X-ray1.60A/B1-138[»]
1OVQNMR-A1-138[»]
ProteinModelPortaliP0A8I1.
SMRiP0A8I1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259241. 196 interactors.
IntActiP0A8I1. 9 interactors.
MINTiMINT-1317755.
STRINGi511145.b2949.

Proteomic databases

PaxDbiP0A8I1.
PRIDEiP0A8I1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75986; AAC75986; b2949.
BAE77012; BAE77012; BAE77012.
GeneIDi947439.
KEGGiecj:JW2916.
eco:b2949.
PATRICi32121308. VBIEscCol129921_3042.

Organism-specific databases

EchoBASEiEB3075.
EcoGeneiEG13290. yqgF.

Phylogenomic databases

eggNOGiENOG4107Y66. Bacteria.
COG0816. LUCA.
HOGENOMiHOG000016954.
InParanoidiP0A8I1.
KOiK07447.
OMAiPMGWTAQ.
PhylomeDBiP0A8I1.

Enzyme and pathway databases

BioCyciEcoCyc:G7525-MONOMER.
ECOL316407:JW2916-MONOMER.
MetaCyc:G7525-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8I1.
PROiP0A8I1.

Family and domain databases

Gene3Di3.30.420.140. 1 hit.
HAMAPiMF_00651. Nuclease_YqgF. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR005227. YqgF.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PfamiPF03652. RuvX. 1 hit.
[Graphical view]
SMARTiSM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00250. RNAse_H_YqgF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYQGF_ECOLI
AccessioniPrimary (citable) accession number: P0A8I1
Secondary accession number(s): P52050, Q2M9P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally suggested to be a nuclease that resolves Holliday junction intermediates during genetic recombination.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.