P0A8H8 (YACG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase inhibitor YacG

Gene names

Name:	yacG
Ordered Locus Names:	b0101, JW5008

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	65 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits all the catalytic activities of DNA gyrase by preventing its interaction with DNA. Acts by binding directly to the C-terminal domain of GyrB, which probably disrupts DNA binding by the gyrase. Ref.6
Cofactor	Binds 1 zinc ion.
Subunit structure	Interacts with GyrB. Ref.6
Sequence similarities	Belongs to the DNA gyrase inhibitor YacG family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA topoisomerase (ATP-hydrolyzing) inhibitor activity Inferred from direct assay Ref.6. Source: EcoCyc zinc ion binding Inferred from direct assay Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 65

65

DNA gyrase inhibitor YacG HAMAP-Rule MF_00649

PRO_0000211695

Sites

Metal binding

9

1

Zinc

Metal binding

12

1

Zinc

Metal binding

28

1

Zinc

Metal binding

32

1

Zinc

Secondary structure

1

.

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65

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Turn

10 – 12

3

Beta strand

15 – 17

3

Beta strand

19 – 23

5

Beta strand

26 – 29

4

Helix

30 – 37

8

Beta strand

40 – 44

5

Beta strand

49 – 51

3

Beta strand

54 – 56

3

Sequences

	Sequence		Length	Mass (Da)
	P0A8H8 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: F2747B62AFE6479D	FASTA	65	7,306
	10 20 30 40 50 60 MSETITVNCP TCGKTVVWGE ISPFRPFCSK RCQLIDLGEW AAEEKRIPSS GDLSESDDWS EEPKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Molecular cloning and nucleotide sequence of the mutT mutator of Escherichia coli that causes A:T to C:G transversion." Akiyama M., Horiuchi T., Sekiguchi M. Mol. Gen. Genet. 206:9-16(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-65. Strain: K12.
[5]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 34-65. Strain: K12.
[6]	"YacG from Escherichia coli is a specific endogenous inhibitor of DNA gyrase." Sengupta S., Nagaraja V. Nucleic Acids Res. 36:4310-4316(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GYRB.
[7]	"NMR structure of the Escherichia coli protein YacG: a novel sequence motif in the zinc-finger family of proteins." Ramelot T.A., Cort J.R., Yee A.A., Semesi A., Edwards A.M., Arrowsmith C.H., Kennedy M.A. Proteins 49:289-293(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC73212.1.
AP009048 Genomic DNA. Translation: BAB96668.2.
X04831 Genomic DNA. No translation available.

PIR

E64732.

RefSeq

NP_414643.1. NC_000913.2.
YP_488405.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LV3	NMR	-	A	1-65	[»]

ProteinModelPortal

P0A8H8.

SMR

P0A8H8. Positions 1-65.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48126N.

IntAct

P0A8H8. 3 interactions.

MINT

MINT-1228215.

STRING

511145.b0101.

Proteomic databases

PaxDb

P0A8H8.

PRIDE

P0A8H8.

Protocols and materials databases

DNASU

945630.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC73212; AAC73212; b0101.
BAB96668; BAB96668; BAB96668.

GeneID

12932890.
945630.

KEGG

ecj:Y75_p0099.
eco:b0101.

PATRIC

32115305. VBIEscCol129921_0104.

Organism-specific databases

EchoBASE

EB2220.

EcoGene

EG12314. yacG.

Phylogenomic databases

eggNOG

COG3024.

HOGENOM

HOG000255917.

KO

K09862.

OMA

KCQMIDF.

ProtClustDB

PRK00418.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12314-MONOMER.
ECOL316407:JW5008-MONOMER.

Gene expression databases

Genevestigator

P0A8H8.

Family and domain databases

Gene3D

3.30.50.10. 1 hit.

HAMAP

MF_00649. DNA_gyrase_inhibitor_YacG.

InterPro

IPR005584. DNA_gyrase_inhibitor_YacG.
IPR013088. Znf_NHR/GATA.
[Graphical view]

Pfam

PF03884. DUF329. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A8H8.

Entry information

Entry name

YACG_ECOLI

Accession

Primary (citable) accession number: P0A8H8
Secondary accession number(s): P36681, P75644, Q8KJQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program