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Protein

DNA gyrase inhibitor YacG

Gene

yacG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits all the catalytic activities of DNA gyrase by preventing its interaction with DNA. Acts by binding directly to the C-terminal domain of GyrB, which probably disrupts DNA binding by the gyrase.UniRule annotation1 Publication

Cofactori

Zn2+Note: Binds 1 zinc ion.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Zinc
Metal bindingi12 – 121Zinc
Metal bindingi28 – 281Zinc
Metal bindingi32 – 321Zinc

GO - Molecular functioni

  • DNA topoisomerase (ATP-hydrolyzing) inhibitor activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG12314-MONOMER.
ECOL316407:JW5008-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase inhibitor YacGUniRule annotation
Gene namesi
Name:yacGUniRule annotation
Ordered Locus Names:b0101, JW5008
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12314. yacG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6565DNA gyrase inhibitor YacGPRO_0000211695Add
BLAST

Proteomic databases

PaxDbiP0A8H8.
PRIDEiP0A8H8.

Interactioni

Subunit structurei

Interacts with GyrB.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261884. 1 interaction.
DIPiDIP-48126N.
IntActiP0A8H8. 3 interactions.
MINTiMINT-1228215.
STRINGi511145.b0101.

Structurei

Secondary structure

1
65
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Turni10 – 123Combined sources
Beta strandi15 – 195Combined sources
Turni23 – 264Combined sources
Beta strandi27 – 293Combined sources
Helixi30 – 4112Combined sources
Beta strandi49 – 513Combined sources
Beta strandi54 – 563Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LV3NMR-A1-65[»]
4TMAX-ray3.30I/J/K/L1-65[»]
ProteinModelPortaliP0A8H8.
SMRiP0A8H8. Positions 1-65.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8H8.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA gyrase inhibitor YacG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41067SF. Bacteria.
COG3024. LUCA.
HOGENOMiHOG000255917.
InParanoidiP0A8H8.
KOiK09862.
OMAiTIVKCPQ.
PhylomeDBiP0A8H8.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
HAMAPiMF_00649. DNA_gyrase_inhibitor_YacG. 1 hit.
InterProiIPR005584. DNA_gyrase_inhibitor_YacG.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF03884. DUF329. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8H8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETITVNCP TCGKTVVWGE ISPFRPFCSK RCQLIDLGEW AAEEKRIPSS
60
GDLSESDDWS EEPKQ
Length:65
Mass (Da):7,306
Last modified:June 7, 2005 - v1
Checksum:iF2747B62AFE6479D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73212.1.
AP009048 Genomic DNA. Translation: BAB96668.2.
X04831 Genomic DNA. No translation available.
PIRiE64732.
RefSeqiNP_414643.1. NC_000913.3.
WP_000005042.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73212; AAC73212; b0101.
BAB96668; BAB96668; BAB96668.
GeneIDi945630.
KEGGiecj:JW5008.
eco:b0101.
PATRICi32115305. VBIEscCol129921_0104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73212.1.
AP009048 Genomic DNA. Translation: BAB96668.2.
X04831 Genomic DNA. No translation available.
PIRiE64732.
RefSeqiNP_414643.1. NC_000913.3.
WP_000005042.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LV3NMR-A1-65[»]
4TMAX-ray3.30I/J/K/L1-65[»]
ProteinModelPortaliP0A8H8.
SMRiP0A8H8. Positions 1-65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261884. 1 interaction.
DIPiDIP-48126N.
IntActiP0A8H8. 3 interactions.
MINTiMINT-1228215.
STRINGi511145.b0101.

Proteomic databases

PaxDbiP0A8H8.
PRIDEiP0A8H8.

Protocols and materials databases

DNASUi945630.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73212; AAC73212; b0101.
BAB96668; BAB96668; BAB96668.
GeneIDi945630.
KEGGiecj:JW5008.
eco:b0101.
PATRICi32115305. VBIEscCol129921_0104.

Organism-specific databases

EchoBASEiEB2220.
EcoGeneiEG12314. yacG.

Phylogenomic databases

eggNOGiENOG41067SF. Bacteria.
COG3024. LUCA.
HOGENOMiHOG000255917.
InParanoidiP0A8H8.
KOiK09862.
OMAiTIVKCPQ.
PhylomeDBiP0A8H8.

Enzyme and pathway databases

BioCyciEcoCyc:EG12314-MONOMER.
ECOL316407:JW5008-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8H8.
PROiP0A8H8.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
HAMAPiMF_00649. DNA_gyrase_inhibitor_YacG. 1 hit.
InterProiIPR005584. DNA_gyrase_inhibitor_YacG.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF03884. DUF329. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYACG_ECOLI
AccessioniPrimary (citable) accession number: P0A8H8
Secondary accession number(s): P36681, P75644, Q8KJQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.