Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Der GTPase-activating protein YihI

Gene

yihI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

A GTPase-activating protein (GAP) that modifies Der/EngA GTPase function, negatively regulating cell growth, probably via ribosome assembly. Stimulates the GTPase activity of Der; a construct missing the first 45 residues is even more stimulatory. Does not stimulate 2 other GTPases (ObgE and Era). Overexpression inhibits cell growth; precursor 16S rRNA accumulates, the 23S rRNA is 6-7 bases longer than usual, and 50S ribosomal subunits are improperly assembled, leading to 45S subunits lacking proteins L9, L18 and L25. Overexpression of Der in the same cells suppresses the 50S subunit assembly defect, corroborating that YihI and Der interact.1 Publication

Miscellaneous

This is a highly hydrophilic protein; there is a stretch of highly charged residues in the N-terminus and a stretch of acidic residues in the C-terminus.

GO - Molecular functioni

  • GTPase activator activity Source: EcoCyc

GO - Biological processi

  • negative regulation of ribosome biogenesis Source: EcoCyc
  • positive regulation of GTPase activity Source: EcoCyc
  • ribosome biogenesis Source: UniProtKB-KW

Keywordsi

Molecular functionGTPase activation
Biological processRibosome biogenesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11835-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Der GTPase-activating protein YihI
Short name:
GAP
Gene namesi
Name:yihI
Ordered Locus Names:b3866, JW3837
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11835. yihI.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Dispensable for cell growth, cells lacking this gene have a shorter lag phase.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65R → A: No change in ability to stimulate Der GTPase. 1 Publication1
Mutagenesisi135R → A: No change in ability to stimulate Der GTPase. 1 Publication1
Mutagenesisi164R → A: No change in ability to stimulate Der GTPase. 1 Publication1
Mutagenesisi167R → A: No change in ability to stimulate Der GTPase. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002095821 – 169Der GTPase-activating protein YihIAdd BLAST169

Proteomic databases

PaxDbiP0A8H6.
PRIDEiP0A8H6.

Expressioni

Inductioni

Highly expressed during the lag phase, not expressed in later phases.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with Der/EngA via the last 78 residues. Interaction with Der occurs at a 1:1 stoichiometry, suggesting the dimer dissociates to interact with Der.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4262990. 15 interactors.
DIPiDIP-47963N.
IntActiP0A8H6. 28 interactors.
MINTiMINT-1218965.
STRINGi316385.ECDH10B_4056.

Structurei

3D structure databases

ProteinModelPortaliP0A8H6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 45Not required for interaction with Der/EngaAdd BLAST45
Regioni17 – 45Arg/Lys richAdd BLAST29
Regioni148 – 157Asp/Glu rich10

Sequence similaritiesi

Belongs to the YihI family.Curated

Phylogenomic databases

eggNOGiENOG4105ZR3. Bacteria.
COG3078. LUCA.
HOGENOMiHOG000002380.
KOiK09894.

Family and domain databases

HAMAPiMF_01058. GAP_YihI. 1 hit.
InterProiView protein in InterPro
IPR007336. Der_GTPase_activator.
PfamiView protein in Pfam
PF04220. YihI. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8H6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSSSNSRS KGHAKARRKT REELDQEARD RKRQKKRRGH APGSRAAGGN
60 70 80 90 100
TTSGSKGQNA PKDPRIGSKT PIPLGVTEKV TKQHKPKSEK PMLSPQAELE
110 120 130 140 150
LLETDERLDA LLERLEAGET LSAEEQSWVD AKLDRIDELM QKLGLSYDDD
160
EEEEEDEKQE DMMRLLRGN
Length:169
Mass (Da):19,059
Last modified:June 7, 2005 - v1
Checksum:i31C1DF0C07AD9D07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16509 Genomic DNA. Translation: BAA03960.1.
L19201 Genomic DNA. Translation: AAB03000.1.
U00096 Genomic DNA. Translation: AAC76863.1.
AP009048 Genomic DNA. Translation: BAE77443.1.
PIRiS40811.
RefSeqiNP_418302.1. NC_000913.3.
WP_001295266.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76863; AAC76863; b3866.
BAE77443; BAE77443; BAE77443.
GeneIDi948363.
KEGGiecj:JW3837.
eco:b3866.
PATRICifig|1411691.4.peg.2847.

Similar proteinsi

Entry informationi

Entry nameiYIHI_ECOLI
AccessioniPrimary (citable) accession number: P0A8H6
Secondary accession number(s): P32130, Q2M8G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries