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Protein

Exodeoxyribonuclease 7 small subunit

Gene

xseB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides. It can also degrade 3' or 5' ss regions extending from the termini of duplex DNA molecules and displaced ss regions.

Catalytic activityi

Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

  • exodeoxyribonuclease VII activity Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciEcoCyc:EG11098-MONOMER.
ECOL316407:JW0412-MONOMER.
MetaCyc:EG11098-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease 7 small subunit (EC:3.1.11.6)
Alternative name(s):
Exodeoxyribonuclease VII small subunit
Short name:
Exonuclease VII small subunit
Gene namesi
Name:xseB
Synonyms:yajE
Ordered Locus Names:b0422, JW0412
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11098. xseB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • exodeoxyribonuclease VII complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 8079Exodeoxyribonuclease 7 small subunitPRO_0000206945Add
BLAST

Proteomic databases

PaxDbiP0A8G9.
PRIDEiP0A8G9.

Interactioni

Subunit structurei

Heterooligomer composed of two different subunits with an approximate ratio of 4 small subunits for 1 large.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
yebVP645034EBI-1116872,EBI-9126792

Protein-protein interaction databases

BioGridi4259844. 55 interactions.
DIPiDIP-48031N.
IntActiP0A8G9. 4 interactions.
STRINGi511145.b0422.

Structurei

3D structure databases

ProteinModelPortaliP0A8G9.
SMRiP0A8G9. Positions 8-66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the XseB family.Curated

Phylogenomic databases

eggNOGiENOG41067QG. Bacteria.
COG1722. LUCA.
HOGENOMiHOG000228798.
InParanoidiP0A8G9.
KOiK03602.
OMAiAPLNDYK.
OrthoDBiEOG69KV31.
PhylomeDBiP0A8G9.

Family and domain databases

Gene3Di1.10.287.1040. 1 hit.
HAMAPiMF_00337. Exonuc_7_S.
InterProiIPR003761. Exonuc_VII_S.
[Graphical view]
PfamiPF02609. Exonuc_VII_S. 1 hit.
[Graphical view]
PIRSFiPIRSF006488. Exonuc_VII_S. 1 hit.
ProDomiPD028235. Exonuc_VII_S. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01280. xseB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKKNEAPAS FEKALSELEQ IVTRLESGDL PLEEALNEFE RGVQLARQGQ
60 70 80
AKLQQAEQRV QILLSDNEDA SLTPFTPDNE
Length:80
Mass (Da):8,952
Last modified:January 23, 2007 - v2
Checksum:i8B10380D442CADF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00694 Genomic DNA. Translation: BAA00598.1.
U82664 Genomic DNA. Translation: AAB40178.1.
U00096 Genomic DNA. Translation: AAC73525.1.
AP009048 Genomic DNA. Translation: BAE76202.1.
PIRiJQ0664.
RefSeqiNP_414956.1. NC_000913.3.
WP_001124935.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73525; AAC73525; b0422.
BAE76202; BAE76202; BAE76202.
GeneIDi945069.
KEGGiecj:JW0412.
eco:b0422.
PATRICi32115993. VBIEscCol129921_0438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00694 Genomic DNA. Translation: BAA00598.1.
U82664 Genomic DNA. Translation: AAB40178.1.
U00096 Genomic DNA. Translation: AAC73525.1.
AP009048 Genomic DNA. Translation: BAE76202.1.
PIRiJQ0664.
RefSeqiNP_414956.1. NC_000913.3.
WP_001124935.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A8G9.
SMRiP0A8G9. Positions 8-66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259844. 55 interactions.
DIPiDIP-48031N.
IntActiP0A8G9. 4 interactions.
STRINGi511145.b0422.

Proteomic databases

PaxDbiP0A8G9.
PRIDEiP0A8G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73525; AAC73525; b0422.
BAE76202; BAE76202; BAE76202.
GeneIDi945069.
KEGGiecj:JW0412.
eco:b0422.
PATRICi32115993. VBIEscCol129921_0438.

Organism-specific databases

EchoBASEiEB1090.
EcoGeneiEG11098. xseB.

Phylogenomic databases

eggNOGiENOG41067QG. Bacteria.
COG1722. LUCA.
HOGENOMiHOG000228798.
InParanoidiP0A8G9.
KOiK03602.
OMAiAPLNDYK.
OrthoDBiEOG69KV31.
PhylomeDBiP0A8G9.

Enzyme and pathway databases

BioCyciEcoCyc:EG11098-MONOMER.
ECOL316407:JW0412-MONOMER.
MetaCyc:EG11098-MONOMER.

Miscellaneous databases

PROiP0A8G9.

Family and domain databases

Gene3Di1.10.287.1040. 1 hit.
HAMAPiMF_00337. Exonuc_7_S.
InterProiIPR003761. Exonuc_VII_S.
[Graphical view]
PfamiPF02609. Exonuc_VII_S. 1 hit.
[Graphical view]
PIRSFiPIRSF006488. Exonuc_VII_S. 1 hit.
ProDomiPD028235. Exonuc_VII_S. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsiTIGR01280. xseB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli."
    Fujisaki S., Hara H., Nishimura Y., Horiuchi K., Nishino T.
    J. Biochem. 108:995-1000(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Diaz D.L., Williams K.R., Chase J.W.
    Unpublished observations (AUG-1995)
    Cited for: PROTEIN SEQUENCE OF 2-22.
  6. Diaz D.L., Chase J.W.
    Unpublished observations (FEB-1994)
    Cited for: IDENTIFICATION OF GENE.
  7. "Subunit structure of Escherichia coli exonuclease VII."
    Vales L.D., Rabin B.A., Chase J.W.
    J. Biol. Chem. 257:8799-8805(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiEX7S_ECOLI
AccessioniPrimary (citable) accession number: P0A8G9
Secondary accession number(s): P22938, Q2MC04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: April 13, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.