Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UvrABC system protein C

Gene

uvrC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG11063-MONOMER.
ECOL316407:JW1898-MONOMER.
MetaCyc:EG11063-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein C
Short name:
Protein UvrC
Alternative name(s):
Excinuclease ABC subunit C
Gene namesi
Name:uvrC
Ordered Locus Names:b1913, JW1898
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11063. uvrC.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • excinuclease repair complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421R → A: Defective in 3' incision but not in 5' incision. 1 Publication
Mutagenesisi399 – 3991D → A or N: Defective in 5' incision but not in 3' incision. 1 Publication
Mutagenesisi438 – 4381D → A or N: Defective in 5' incision but not in 3' incision. 1 Publication
Mutagenesisi466 – 4661D → A or N: Defective in 5' incision but not in 3' incision. 1 Publication
Mutagenesisi538 – 5381H → F or Y: Defective in 5' incision but not in 3' incision. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610UvrABC system protein CPRO_0000138300Add
BLAST

Proteomic databases

EPDiP0A8G0.
PaxDbiP0A8G0.
PRIDEiP0A8G0.

Interactioni

Subunit structurei

Interacts with UvrB in an incision complex.

Protein-protein interaction databases

BioGridi4260379. 105 interactions.
DIPiDIP-47875N.
IntActiP0A8G0. 45 interactions.
MINTiMINT-1221235.
STRINGi511145.b1913.

Structurei

Secondary structure

1
610
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi557 – 5604Combined sources
Beta strandi565 – 5673Combined sources
Helixi568 – 5769Combined sources
Helixi579 – 5846Combined sources
Helixi587 – 5904Combined sources
Beta strandi593 – 5953Combined sources
Helixi599 – 60810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KFTNMR-A554-610[»]
ProteinModelPortaliP0A8G0.
SMRiP0A8G0. Positions 8-100, 379-610.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8G0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9479GIY-YIGAdd
BLAST
Domaini204 – 23936UVRAdd
BLAST

Sequence similaritiesi

Belongs to the UvrC family.Curated
Contains 1 GIY-YIG domain.Curated
Contains 1 UVR domain.Curated

Phylogenomic databases

eggNOGiENOG4105CII. Bacteria.
COG0322. LUCA.
HOGENOMiHOG000279961.
InParanoidiP0A8G0.
KOiK03703.
OMAiLFPIRQC.
OrthoDBiEOG6K13R9.
PhylomeDBiP0A8G0.

Family and domain databases

Gene3Di3.40.1440.10. 1 hit.
4.10.860.10. 1 hit.
HAMAPiMF_00203. UvrC.
InterProiIPR027299. GIY-YIG_dom.
IPR000305. GIY-YIG_SF.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR010994. RuvA_2-like.
IPR001943. UVR_dom.
IPR004791. UvrC.
IPR001162. UvrC_homol_region.
[Graphical view]
PfamiPF01541. GIY-YIG. 1 hit.
PF02151. UVR. 1 hit.
PF08459. UvrC_HhH_N. 1 hit.
[Graphical view]
SMARTiSM00465. GIYc. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF82771. SSF82771. 1 hit.
TIGRFAMsiTIGR00194. uvrC. 1 hit.
PROSITEiPS50164. GIY_YIG. 1 hit.
PS50151. UVR. 1 hit.
PS50165. UVRC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8G0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQFDAKAF LKTVTSQPGV YRMYDAGGTV IYVGKAKDLK KRLSSYFRSN
60 70 80 90 100
LASRKTEALV AQIQQIDVTV THTETEALLL EHNYIKLYQP RYNVLLRDDK
110 120 130 140 150
SYPFIFLSGD THPRLAMHRG AKHAKGEYFG PFPNGYAVRE TLALLQKIFP
160 170 180 190 200
IRQCENSVYR NRSRPCLQYQ IGRCLGPCVE GLVSEEEYAQ QVEYVRLFLS
210 220 230 240 250
GKDDQVLTQL ISRMETASQN LEFEEAARIR DQIQAVRRVT EKQFVSNTGD
260 270 280 290 300
DLDVIGVAFD AGMACVHVLF IRQGKVLGSR SYFPKVPGGT ELSEVVETFV
310 320 330 340 350
GQFYLQGSQM RTLPGEILLD FNLSDKTLLA DSLSELAGRK INVQTKPRGD
360 370 380 390 400
RARYLKLART NAATALTSKL SQQSTVHQRL TALASVLKLP EVKRMECFDI
410 420 430 440 450
SHTMGEQTVA SCVVFDANGP LRAEYRRYNI TGITPGDDYA AMNQVLRRRY
460 470 480 490 500
GKAIDDSKIP DVILIDGGKG QLAQAKNVFA ELDVSWDKNH PLLLGVAKGA
510 520 530 540 550
DRKAGLETLF FEPEGEGFSL PPDSPALHVI QHIRDESHDH AIGGHRKKRA
560 570 580 590 600
KVKNTSSLET IEGVGPKRRQ MLLKYMGGLQ GLRNASVEEI AKVPGISQGL
610
AEKIFWSLKH
Length:610
Mass (Da):68,188
Last modified:June 7, 2005 - v1
Checksum:iF76766477C9ABCB6
GO

Sequence cautioni

The sequence CAA27329.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti291 – 2911E → K (PubMed:6330676).Curated
Sequence conflicti291 – 2911E → K (PubMed:3515318).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03691 Genomic DNA. Translation: CAA27329.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74980.2.
AP009048 Genomic DNA. Translation: BAA15733.1.
M24615 Genomic DNA. Translation: AAA24756.1.
X05398 Genomic DNA. Translation: CAA28983.1.
M12299 Genomic DNA. Translation: AAA98753.1.
PIRiF64954. BVECUC.
RefSeqiNP_416423.4. NC_000913.3.
WP_001283421.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74980; AAC74980; b1913.
BAA15733; BAA15733; BAA15733.
GeneIDi947203.
KEGGiecj:JW1898.
eco:b1913.
PATRICi32119159. VBIEscCol129921_1994.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03691 Genomic DNA. Translation: CAA27329.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74980.2.
AP009048 Genomic DNA. Translation: BAA15733.1.
M24615 Genomic DNA. Translation: AAA24756.1.
X05398 Genomic DNA. Translation: CAA28983.1.
M12299 Genomic DNA. Translation: AAA98753.1.
PIRiF64954. BVECUC.
RefSeqiNP_416423.4. NC_000913.3.
WP_001283421.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KFTNMR-A554-610[»]
ProteinModelPortaliP0A8G0.
SMRiP0A8G0. Positions 8-100, 379-610.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260379. 105 interactions.
DIPiDIP-47875N.
IntActiP0A8G0. 45 interactions.
MINTiMINT-1221235.
STRINGi511145.b1913.

Proteomic databases

EPDiP0A8G0.
PaxDbiP0A8G0.
PRIDEiP0A8G0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74980; AAC74980; b1913.
BAA15733; BAA15733; BAA15733.
GeneIDi947203.
KEGGiecj:JW1898.
eco:b1913.
PATRICi32119159. VBIEscCol129921_1994.

Organism-specific databases

EchoBASEiEB1056.
EcoGeneiEG11063. uvrC.

Phylogenomic databases

eggNOGiENOG4105CII. Bacteria.
COG0322. LUCA.
HOGENOMiHOG000279961.
InParanoidiP0A8G0.
KOiK03703.
OMAiLFPIRQC.
OrthoDBiEOG6K13R9.
PhylomeDBiP0A8G0.

Enzyme and pathway databases

BioCyciEcoCyc:EG11063-MONOMER.
ECOL316407:JW1898-MONOMER.
MetaCyc:EG11063-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8G0.
PROiP0A8G0.

Family and domain databases

Gene3Di3.40.1440.10. 1 hit.
4.10.860.10. 1 hit.
HAMAPiMF_00203. UvrC.
InterProiIPR027299. GIY-YIG_dom.
IPR000305. GIY-YIG_SF.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR010994. RuvA_2-like.
IPR001943. UVR_dom.
IPR004791. UvrC.
IPR001162. UvrC_homol_region.
[Graphical view]
PfamiPF01541. GIY-YIG. 1 hit.
PF02151. UVR. 1 hit.
PF08459. UvrC_HhH_N. 1 hit.
[Graphical view]
SMARTiSM00465. GIYc. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF82771. SSF82771. 1 hit.
TIGRFAMsiTIGR00194. uvrC. 1 hit.
PROSITEiPS50164. GIY_YIG. 1 hit.
PS50151. UVR. 1 hit.
PS50165. UVRC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of the E. coli uvrC gene and protein."
    Sancar G.B., Sancar A., Rupp W.D.
    Nucleic Acids Res. 12:4593-4608(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Multiple control elements for the uvrC gene unit of Escherichia coli."
    Sharma S., Stark T.F., Beattie W.G., Moses R.E.
    Nucleic Acids Res. 14:2301-2318(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Regulation of the Escherichia coli excision repair gene uvrC. Overlap between the uvrC structural gene and the region coding for a 24 kD protein."
    Moolenaar G.F., van Sluis C.A., Backendorf C., van de Putte P.
    Nucleic Acids Res. 15:4273-4289(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
  7. "Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli."
    Gopalakrishnan A.S., Chen Y.-C., Temkin M., Dowhan W.
    J. Biol. Chem. 261:1329-1338(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 604-610.
  8. "Active site of (A)BC excinuclease. I. Evidence for 5' incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466, and His538 residues."
    Lin J.-J., Sancar A.
    J. Biol. Chem. 267:17688-17692(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-399; ASP-438; ASP-466 AND HIS-538.
    Strain: K12.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  10. "Catalytic sites for 3' and 5' incision of Escherichia coli nucleotide excision repair are both located in UvrC."
    Verhoeven E.E., van Kesteren M., Moolenaar G.F., Visse R., Goosen N.
    J. Biol. Chem. 275:5120-5123(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-42.

Entry informationi

Entry nameiUVRC_ECOLI
AccessioniPrimary (citable) accession number: P0A8G0
Secondary accession number(s): P07028, P76311, Q8XBD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 7, 2005
Last modified: April 13, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.