Reviewed, UniProtKB/Swiss-Prot P0A8G0 (UVRC_ECOLI)

Names and origin

Protein names

Recommended name:
    UvrABC system protein C
      Short name=Protein uvrC
Alternative name(s):
    Excinuclease ABC subunit C

Gene names

Name:	uvrC
Ordered Locus Names:	b1913, JW1898

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision. Ref.8 Ref.9
Subunit structure	Interacts with uvrB in an incision complex. HAMAP MF_00203
Subcellular location	Cytoplasm. HAMAP MF_00203
Sequence similarities	Belongs to the uvrC family. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair SOS response
Cellular component	Cytoplasm
Molecular function	Excision nuclease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	SOS response Inferred from electronic annotation. Source: HAMAP nucleotide-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell excinuclease repair complex Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP excinuclease ABC activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	UvrABC system protein C HAMAP MF_00203		PRO_0000138300
Regions
	Domain	204 – 239	36	UVR
Experimental info
	Mutagenesis	42	1	R → A: Defective in 3' incision but not in 5' incision. Ref.9
	Mutagenesis	399	1	D → A or N: Defective in 5' incision but not in 3' incision. Ref.8
	Mutagenesis	438	1	D → A or N: Defective in 5' incision but not in 3' incision. Ref.8
	Mutagenesis	466	1	D → A or N: Defective in 5' incision but not in 3' incision. Ref.8
	Mutagenesis	538	1	H → F or Y: Defective in 5' incision but not in 3' incision. Ref.8
	Sequence conflict	291	1	E → K Ref.1
	Sequence conflict	291	1	E → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A8G0-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: F76766477C9ABCB6	FASTA	610	68,188
	10 20 30 40 50 60 MSDQFDAKAF LKTVTSQPGV YRMYDAGGTV IYVGKAKDLK KRLSSYFRSN LASRKTEALV 70 80 90 100 110 120 AQIQQIDVTV THTETEALLL EHNYIKLYQP RYNVLLRDDK SYPFIFLSGD THPRLAMHRG 130 140 150 160 170 180 AKHAKGEYFG PFPNGYAVRE TLALLQKIFP IRQCENSVYR NRSRPCLQYQ IGRCLGPCVE 190 200 210 220 230 240 GLVSEEEYAQ QVEYVRLFLS GKDDQVLTQL ISRMETASQN LEFEEAARIR DQIQAVRRVT 250 260 270 280 290 300 EKQFVSNTGD DLDVIGVAFD AGMACVHVLF IRQGKVLGSR SYFPKVPGGT ELSEVVETFV 310 320 330 340 350 360 GQFYLQGSQM RTLPGEILLD FNLSDKTLLA DSLSELAGRK INVQTKPRGD RARYLKLART 370 380 390 400 410 420 NAATALTSKL SQQSTVHQRL TALASVLKLP EVKRMECFDI SHTMGEQTVA SCVVFDANGP 430 440 450 460 470 480 LRAEYRRYNI TGITPGDDYA AMNQVLRRRY GKAIDDSKIP DVILIDGGKG QLAQAKNVFA 490 500 510 520 530 540 ELDVSWDKNH PLLLGVAKGA DRKAGLETLF FEPEGEGFSL PPDSPALHVI QHIRDESHDH 550 560 570 580 590 600 AIGGHRKKRA KVKNTSSLET IEGVGPKRRQ MLLKYMGGLQ GLRNASVEEI AKVPGISQGL 610 AEKIFWSLKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequences of the E. coli uvrC gene and protein." Sancar G.B., Sancar A., Rupp W.D. Nucleic Acids Res. 12:4593-4608(1984) [PubMed: 6330676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Multiple control elements for the uvrC gene unit of Escherichia coli." Sharma S., Stark T.F., Beattie W.G., Moses R.E. Nucleic Acids Res. 14:2301-2318(1986) [PubMed: 3515318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Regulation of the Escherichia coli excision repair gene uvrC. Overlap between the uvrC structural gene and the region coding for a 24 kD protein." Moolenaar G.F., van Sluis C.A., Backendorf C., van de Putte P. Nucleic Acids Res. 15:4273-4289(1987) [PubMed: 3295776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-28.
[7]	"Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli." Gopalakrishnan A.S., Chen Y.-C., Temkin M., Dowhan W. J. Biol. Chem. 261:1329-1338(1986) [PubMed: 3003065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 604-610.
[8]	"Active site of (A)BC excinuclease. I. Evidence for 5' incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466, and His538 residues." Lin J.-J., Sancar A. J. Biol. Chem. 267:17688-17692(1992) [PubMed: 1387639] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-399; ASP-438; ASP-466 AND HIS-538. Strain: K12.
[9]	"Catalytic sites for 3' and 5' incision of Escherichia coli nucleotide excision repair are both located in UvrC." Verhoeven E.E., van Kesteren M., Moolenaar G.F., Visse R., Goosen N. J. Biol. Chem. 275:5120-5123(2000) [PubMed: 10671556] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ARG-42.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X03691 Genomic DNA. Translation: CAA27329.1. Different initiation.
U00096 Genomic DNA. Translation: AAC74980.1. Different initiation.
AP009048 Genomic DNA. Translation: BAA15733.1.
M24615 Genomic DNA. Translation: AAA24756.1.
X05398 Genomic DNA. Translation: CAA28983.1.
M12299 Genomic DNA. Translation: AAA98753.1.

PIR

BVECUC. F64954.

RefSeq

AP_002528.1.
NP_416423.4.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1KFT	NMR	-	A	554-610	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0A8G0.

2-D gel databases

ECO2DBASE

I065.0. 6TH EDITION.

Genome annotation databases

GeneID

947203.

GenomeReviews

Gene locus JW1898 in contig AP009048_GR.
Gene locus b1913 in contig U00096_GR.

KEGG

ecj:JW1898.
eco:b1913.

Organism-specific databases

EchoBASE

EB1056.

EcoGene

EG11063. uvrC.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A8G0.

OMA

ESHRFAI.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11063-MON.
MetaCyc:EG11063-MON.

Gene expression databases

Genevestigator

P0A8G0.

Family and domain databases

HAMAP

MF_00203.
[Tree]

InterPro

IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR001943. UvrB/C.
IPR004791. UvrC_ABC.
IPR001162. UvrC_C.
IPR000305. UvrC_N.
[Graphical view]

Pfam

PF01541. GIY-YIG. 1 hit.
PF00633. HHH. 2 hits.
PF02151. UVR. 1 hit.
PF08459. UvrC_HhH_N. 1 hit.
[Graphical view]

ProDom

PD005870. UvrC_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00465. GIYc. 1 hit.
SM00278. HhH1. 2 hits.
[Graphical view]

TIGRFAMs

TIGR00194. uvrC. 1 hit.

PROSITE

PS50151. UVR. 1 hit.
PS50164. UVRC_1. 1 hit.
PS50165. UVRC_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UVRC_ECOLI

Accession

Primary (citable) accession number: P0A8G0
Secondary accession number(s): P07028, P76311, Q8XBD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)