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Protein

UvrABC system protein B

Gene

uvrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11062-MONOMER.
ECOL316407:JW0762-MONOMER.
MetaCyc:EG11062-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein B
Short name:
Protein UvrB
Alternative name(s):
Excinuclease ABC subunit B
Gene namesi
Name:uvrB
Ordered Locus Names:b0779, JW0762
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11062. uvrB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • excinuclease repair complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi95 – 96YY → AA: Defective in DNA-unwinding activity. 1 Publication2
Mutagenesisi101Y → A: Defective in DNA-unwinding activity; when associated with A-108. 1 Publication1
Mutagenesisi108F → A: Defective in DNA-unwinding activity; when associated with A-101. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001383902 – 673UvrABC system protein BAdd BLAST672

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei630 – 631CleavageSequence analysis2

Proteomic databases

PaxDbiP0A8F8.
PRIDEiP0A8F8.

Expressioni

Inductioni

Induced 1.5-fold by hydroxyurea.1 Publication

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.

Binary interactionsi

WithEntry#Exp.IntActNotes
seqAP0AFY82EBI-552176,EBI-552553
ugdP763732EBI-552176,EBI-1120497

Protein-protein interaction databases

BioGridi4259957. 128 interactors.
DIPiDIP-48012N.
IntActiP0A8F8. 31 interactors.
MINTiMINT-1244062.
STRINGi511145.b0779.

Structurei

Secondary structure

1673
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi629 – 648Combined sources20
Helixi654 – 671Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E52NMR-A/B619-673[»]
1QOJX-ray3.00A/B619-673[»]
ProteinModelPortaliP0A8F8.
SMRiP0A8F8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A8F8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 415Helicase ATP-bindingAdd BLAST390
Domaini431 – 597Helicase C-terminalAdd BLAST167
Domaini633 – 668UVRAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92 – 115Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.

Sequence similaritiesi

Belongs to the UvrB family.Curated
Contains 1 helicase ATP-binding domain.Curated
Contains 1 helicase C-terminal domain.Curated
Contains 1 UVR domain.Curated

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
InParanoidiP0A8F8.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiP0A8F8.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A8F8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA
60 70 80 90 100
NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA
110 120 130 140 150
YVPSSDTFIE KDASVNEHIE QMRLSATKAM LERRDVVVVA SVSAIYGLGD
160 170 180 190 200
PDLYLKMMLH LTVGMIIDQR AILRRLAELQ YARNDQAFQR GTFRVRGEVI
210 220 230 240 250
DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR FTIYPKTHYV
260 270 280 290 300
TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL
310 320 330 340 350
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM
360 370 380 390 400
YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE
410 420 430 440 450
LEKSGGDVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL
460 470 480 490 500
VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL
510 520 530 540 550
VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA
560 570 580 590 600
ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG
610 620 630 640 650
QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL
660 670
EFEEAAQIRD QLHQLRELFI AAS
Length:673
Mass (Da):76,226
Last modified:January 23, 2007 - v2
Checksum:i2F172045344FDAD7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti477H → R in CAA27314 (PubMed:3515321).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03678 Genomic DNA. Translation: CAA27314.1.
X03722 Genomic DNA. Translation: CAA27357.1.
U00096 Genomic DNA. Translation: AAC73866.1.
AP009048 Genomic DNA. Translation: BAA35437.2.
PIRiA93613. BVECUB.
RefSeqiNP_415300.1. NC_000913.3.
WP_000042533.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73866; AAC73866; b0779.
BAA35437; BAA35437; BAA35437.
GeneIDi945385.
KEGGiecj:JW0762.
eco:b0779.
PATRICi32116759. VBIEscCol129921_0805.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03678 Genomic DNA. Translation: CAA27314.1.
X03722 Genomic DNA. Translation: CAA27357.1.
U00096 Genomic DNA. Translation: AAC73866.1.
AP009048 Genomic DNA. Translation: BAA35437.2.
PIRiA93613. BVECUB.
RefSeqiNP_415300.1. NC_000913.3.
WP_000042533.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E52NMR-A/B619-673[»]
1QOJX-ray3.00A/B619-673[»]
ProteinModelPortaliP0A8F8.
SMRiP0A8F8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259957. 128 interactors.
DIPiDIP-48012N.
IntActiP0A8F8. 31 interactors.
MINTiMINT-1244062.
STRINGi511145.b0779.

Proteomic databases

PaxDbiP0A8F8.
PRIDEiP0A8F8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73866; AAC73866; b0779.
BAA35437; BAA35437; BAA35437.
GeneIDi945385.
KEGGiecj:JW0762.
eco:b0779.
PATRICi32116759. VBIEscCol129921_0805.

Organism-specific databases

EchoBASEiEB1055.
EcoGeneiEG11062. uvrB.

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
InParanoidiP0A8F8.
KOiK03702.
OMAiQEYVDRM.
PhylomeDBiP0A8F8.

Enzyme and pathway databases

BioCyciEcoCyc:EG11062-MONOMER.
ECOL316407:JW0762-MONOMER.
MetaCyc:EG11062-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A8F8.
PROiP0A8F8.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiIPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVRB_ECOLI
AccessioniPrimary (citable) accession number: P0A8F8
Secondary accession number(s): P07025
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

According to PubMed:3515321, a cleaved form of the protein was observed that resulted from the removal of about 40 amino acids from the C-terminus of the protein. The exact cleavage site being unknown, it was proposed to be between Lys-630 and Ala-631. There was no indication that cleavage occured in vivo and therefore it is not known if it has any physiological significance.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.