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Protein

Uridine kinase

Gene

udk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uridine kinase (udk)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine kinase (udk)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • cytidine kinase activity Source: EcoCyc
  • uridine kinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:UDK-MONOMER.
ECOL316407:JW2051-MONOMER.
MetaCyc:UDK-MONOMER.
UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine kinase (EC:2.7.1.48)
Alternative name(s):
Cytidine monophosphokinase
Uridine monophosphokinase
Gene namesi
Name:udk
Ordered Locus Names:b2066, JW2051
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11701. udk.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Uridine kinasePRO_0000164470Add
BLAST

Proteomic databases

EPDiP0A8F4.
PaxDbiP0A8F4.
PRIDEiP0A8F4.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4259683. 7 interactions.
DIPiDIP-36012N.
IntActiP0A8F4. 12 interactions.
STRINGi511145.b2066.

Structurei

3D structure databases

ProteinModelPortaliP0A8F4.
SMRiP0A8F4. Positions 10-207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBI. Bacteria.
COG0572. LUCA.
HOGENOMiHOG000262756.
InParanoidiP0A8F4.
KOiK00876.
OMAiEHTRTTE.
OrthoDBiEOG6KMBCH.
PhylomeDBiP0A8F4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00551. Uridine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR000764. Uridine_kinase-like.
IPR026008. Uridine_kinase_.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A8F4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQSHQCVI IGIAGASASG KSLIASTLYR ELREQVGDEH IGVIPEDCYY
60 70 80 90 100
KDQSHLSMEE RVKTNYDHPS AMDHSLLLEH LQALKRGSAI DLPVYSYVEH
110 120 130 140 150
TRMKETVTVE PKKVIILEGI LLLTDARLRD ELNFSIFVDT PLDICLMRRI
160 170 180 190 200
KRDVNERGRS MDSVMAQYQK TVRPMFLQFI EPSKQYADII VPRGGKNRIA
210
IDILKAKISQ FFE
Length:213
Mass (Da):24,353
Last modified:June 7, 2005 - v1
Checksum:i09F6F5FBCCF1A232
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71492 Genomic DNA. Translation: CAA50593.1.
U00096 Genomic DNA. Translation: AAC75127.2.
AP009048 Genomic DNA. Translation: BAA15919.1.
PIRiA64973.
RefSeqiNP_416570.2. NC_000913.3.
WP_001295424.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75127; AAC75127; b2066.
BAA15919; BAA15919; BAA15919.
GeneIDi946597.
KEGGiecj:JW2051.
eco:b2066.
PATRICi32119465. VBIEscCol129921_2143.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X71492 Genomic DNA. Translation: CAA50593.1.
U00096 Genomic DNA. Translation: AAC75127.2.
AP009048 Genomic DNA. Translation: BAA15919.1.
PIRiA64973.
RefSeqiNP_416570.2. NC_000913.3.
WP_001295424.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A8F4.
SMRiP0A8F4. Positions 10-207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259683. 7 interactions.
DIPiDIP-36012N.
IntActiP0A8F4. 12 interactions.
STRINGi511145.b2066.

Proteomic databases

EPDiP0A8F4.
PaxDbiP0A8F4.
PRIDEiP0A8F4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75127; AAC75127; b2066.
BAA15919; BAA15919; BAA15919.
GeneIDi946597.
KEGGiecj:JW2051.
eco:b2066.
PATRICi32119465. VBIEscCol129921_2143.

Organism-specific databases

EchoBASEiEB1652.
EcoGeneiEG11701. udk.

Phylogenomic databases

eggNOGiENOG4105DBI. Bacteria.
COG0572. LUCA.
HOGENOMiHOG000262756.
InParanoidiP0A8F4.
KOiK00876.
OMAiEHTRTTE.
OrthoDBiEOG6KMBCH.
PhylomeDBiP0A8F4.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.
BioCyciEcoCyc:UDK-MONOMER.
ECOL316407:JW2051-MONOMER.
MetaCyc:UDK-MONOMER.

Miscellaneous databases

PROiP0A8F4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00551. Uridine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR000764. Uridine_kinase-like.
IPR026008. Uridine_kinase_.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Location of the udk gene on the physical map of Escherichia coli."
    Neuhard J., Tarpo L.
    J. Bacteriol. 175:5742-5743(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiURK_ECOLI
AccessioniPrimary (citable) accession number: P0A8F4
Secondary accession number(s): P31218, P78085, Q8X7L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 6, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.