Reviewed, UniProtKB/Swiss-Prot P0A8F4 (URK_ECOLI)

Names and origin

Protein names

Recommended name:
    Uridine kinase
    EC=2.7.1.48
Alternative name(s):
    Uridine monophosphokinase
    Cytidine monophosphokinase

Gene names

Name:	udk
Ordered Locus Names:	b2066, JW2051

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + uridine = ADP + UMP. HAMAP MF_00551 ATP + cytidine = ADP + CMP. HAMAP MF_00551
Pathway	Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3. HAMAP MF_00551 Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1. HAMAP MF_00551
Subunit structure	Homotetramer. HAMAP MF_00551
Subcellular location	Cytoplasm. HAMAP MF_00551
Sequence similarities	Belongs to the uridine kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine salvage Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro uridine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Uridine kinase HAMAP MF_00551		PRO_0000164470
Regions
	Nucleotide binding	15 – 22	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A8F4-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 09F6F5FBCCF1A232	FASTA	213	24,353
	10 20 30 40 50 60 MTDQSHQCVI IGIAGASASG KSLIASTLYR ELREQVGDEH IGVIPEDCYY KDQSHLSMEE 70 80 90 100 110 120 RVKTNYDHPS AMDHSLLLEH LQALKRGSAI DLPVYSYVEH TRMKETVTVE PKKVIILEGI 130 140 150 160 170 180 LLLTDARLRD ELNFSIFVDT PLDICLMRRI KRDVNERGRS MDSVMAQYQK TVRPMFLQFI 190 200 210 EPSKQYADII VPRGGKNRIA IDILKAKISQ FFE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Location of the udk gene on the physical map of Escherichia coli." Neuhard J., Tarpo L. J. Bacteriol. 175:5742-5743(1993) [PubMed: 8366067] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71492 Genomic DNA. Translation: CAA50593.1. U00096 Genomic DNA. Translation: AAC75127.1. Different initiation. AP009048 Genomic DNA. Translation: BAA15919.1.
PIR	A64973.
RefSeq	AP_002666.1. NP_416570.2.
3D structure databases
ModBase	Search...
PTM databases
PhosSite	P0A8F4.
Genome annotation databases
GeneID	946597.
GenomeReviews	Gene locus JW2051 in contig AP009048_GR. Gene locus b2066 in contig U00096_GR.
KEGG	ecj:JW2051. eco:b2066.
Organism-specific databases
EchoBASE	EB1652.
EcoGene	EG11701. udk.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A8F4.
OMA	P0A8F4. NQFIEPT.
Enzyme and pathway databases
BioCyc	EcoCyc:UDK-MON. MetaCyc:UDK-MON.
Family and domain databases
HAMAP	MF_00551. [Tree]
InterPro	IPR006083. PRK_URK. IPR000764. Uridine_kinase. [Graphical view]
PANTHER	PTHR10285:SF6. Uridine_kin. 1 hit.
Pfam	PF00485. PRK. 1 hit. [Graphical view]
PRINTS	PR00988. URIDINKINASE.
TIGRFAMs	TIGR00235. udk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

URK_ECOLI

Accession

Primary (citable) accession number: P0A8F4
Secondary accession number(s): P31218, P78085, Q8X7L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)