Reviewed, UniProtKB/Swiss-Prot P0A8D0 (NRDR_ECOLI)

Names and origin

Protein names

Recommended name:
    Transcriptional repressor nrdR

Gene names

Name:	nrdR
Synonyms:	ybaD
Ordered Locus Names:	b0413, JW0403

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Represses transcription of the class Ib RNR genes nrdHIEF but has much smaller effect on transcription of the class Ia RNR genes nrdAB and class III RNR genes nrdDG. By binding to nrdR boxes in the promoter regions to alter promoter activity, nrdR differentially regulates nrdAB, nrdHIEF and nrdD transcription in aerobic growth. Ref.6
Cofactor	Binds 1 zinc ion By similarity. HAMAP MF_00440
Sequence similarities	Belongs to the nrdR family. Contains 1 ATP-cone domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of transcription Ref.6 Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded DNA binding Ref.6 Inferred from direct assay. Source: UniProtKB transcription repressor activity Ref.6 Inferred from expression pattern. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Transcriptional repressor nrdR HAMAP MF_00440		PRO_0000182296
Regions
	Domain	49 – 139	91	ATP-cone
	Zinc finger	3 – 34	32	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A8D0-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: EFF99DD555EF2D26	FASTA	149	17,229
	10 20 30 40 50 60 MHCPFCFAVD TKVIDSRLVG EGSSVRRRRQ CLVCNERFTT FEVAELVMPR VVKSNDVREP 70 80 90 100 110 120 FNEEKLRSGM LRALEKRPVS SDDVEMAINH IKSQLRATGE REVPSKMIGN LVMEQLKKLD 130 140 KVAYIRFASV YRSFEDIKEF GEEIARLED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insertional disruption of the nusB (ssyB) gene leads to cold-sensitive growth of Escherichia coli and suppression of the secY24 mutation." Taura T., Ueguchi C., Shiba K., Ito K. Mol. Gen. Genet. 234:429-432(1992) [PubMed: 1406588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Phylogeny and distribution of nrdR orthologous genes in bacteria." Borovok I., Aharonowitz Y., Cohen G. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"NrdR controls differential expression of the Escherichia coli ribonucleotide reductase genes." Torrents E., Grinberg I., Gorovitz-Harris B., Lundstroem H., Borovok I., Aharonowitz Y., Sjoeberg B.M., Cohen G. J. Bacteriol. 189:5012-5021(2007) [PubMed: 17496099] [Abstract] Cited for: FUNCTION AS A REGULATOR OF RIBONUCLEOTIDE REDUCTASE GENES. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64395 Genomic DNA. Translation: CAA45734.1. AM076828 Genomic DNA. Translation: CAJ28592.1. U82664 Genomic DNA. Translation: AAB40169.1. U00096 Genomic DNA. Translation: AAC73516.1. AP009048 Genomic DNA. Translation: BAE76193.1.
PIR	S26200.
RefSeq	AP_001063.1. NP_414947.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P0A8D0.
Genome annotation databases
GeneID	947437.
GenomeReviews	Gene locus JW0403 in contig AP009048_GR. Gene locus b0413 in contig U00096_GR.
KEGG	ecj:JW0403. eco:b0413.
Organism-specific databases
EchoBASE	EB1296.
EcoGene	EG11320. nrdR.
CMR	Search...
Phylogenomic databases
eggNOG	COG1327.
HOGENOM	HBG351755.
OMA	FASVYRD.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11320-MONOMER. ECOL168927:B0413-MONOMER.
Gene expression databases
Genevestigator	P0A8D0.
Family and domain databases
HAMAP	MF_00440. NrdR. [Tree]
InterPro	IPR005144. ATP-cone. IPR003796. RNR_NrdR-like. [Graphical view]
Pfam	PF03477. ATP-cone. 1 hit. [Graphical view]
TIGRFAMs	TIGR00244. RNR_NrdR-like. 1 hit.
PROSITE	PS51161. ATP_CONE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRDR_ECOLI

Accession

Primary (citable) accession number: P0A8D0
Secondary accession number(s): P25538, Q2MC13, Q3ZUB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)