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P0A8A4 (PSRP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate synthase regulatory protein

Short name=PEP synthase regulatory protein
Short name=PSRP
EC=2.7.11.-
EC=2.7.4.-
Alternative name(s):
Pyruvate, water dikinase regulatory protein
Gene names
Name:ppsR
Synonyms:ydiA
Ordered Locus Names:b1703, JW1693
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length277 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the phosphoenolpyruvate synthase (PEPS) by catalyzing its phosphorylation/dephosphorylation. Ref.7

Catalytic activity

ADP + [Pyruvate, water dikinase] = AMP + [pyruvate, water dikinase] phosphate. Ref.7

[Pyruvate, water dikinase] phosphate + phosphate = [pyruvate, water dikinase] + diphosphate. Ref.7

Subunit structure

Homotetramer. Ref.7

Sequence similarities

Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PSRP subfamily.

Sequence caution

The sequence M69116 differs from that shown. Reason: Frameshift at positions 64 and 90.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

rluDP336432EBI-548302,EBI-558026

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 277277Phosphoenolpyruvate synthase regulatory protein HAMAP-Rule MF_01062
PRO_0000196659

Regions

Nucleotide binding157 – 1648ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P0A8A4 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 25508DEEC2E342CA

FASTA27731,211
        10         20         30         40         50         60 
MDNAVDRHVF YISDGTAITA EVLGHAVMSQ FPVTISSITL PFVENESRAR AVKDQIDAIY 

        70         80         90        100        110        120 
HQTGVRPLVF YSIVLPEIRA IILQSEGFCQ DIVQALVAPL QQEMKLDPTP IAHRTHGLNP 

       130        140        150        160        170        180 
NNLNKYDARI AAIDYTLAHD DGISLRNLDQ AQVILLGVSR CGKTPTSLYL AMQFGIRAAN 

       190        200        210        220        230        240 
YPFIADDMDN LVLPASLKPL QHKLFGLTID PERLAAIREE RRENSRYASL RQCRMEVAEV 

       250        260        270 
EALYRKNQIP WINSTNYSVE EIATKILDIM GLSRRMY 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The cloning and sequence of the E. coli pps gene."
Holzschu D.L., McElver J.A., Liao C.C., Berry A.
Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-107.
Strain: K12.
[5]"Two promoters control the aroH gene of Escherichia coli."
Hudson G.S., Rellos P., Davidson B.E.
Gene 102:87-91(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 107-277.
[6]"Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli."
Zurawski G., Gunsalus R.P., Brown K.D., Yanofsky C.
J. Mol. Biol. 145:47-73(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 153-277.
[7]"Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase--Pi-dependent pyrophosphorylase from bacteria."
Burnell J.N.
BMC Biochem. 11:1-1(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74773.1.
AP009048 Genomic DNA. Translation: BAA15472.2.
M69116 Genomic DNA. No translation available.
M38266 Genomic DNA. Translation: AAA23496.1.
V00261 Genomic DNA. No translation available.
PIRQQECAD. G64928.
RefSeqNP_416218.1. NC_000913.2.
YP_489965.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A8A4.
SMRP0A8A4. Positions 203-270.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47885N.
IntActP0A8A4. 10 interactions.
MINTMINT-1236315.
STRING511145.b1703.

Proteomic databases

PaxDbP0A8A4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74773; AAC74773; b1703.
BAA15472; BAA15472; BAA15472.
GeneID12931294.
946207.
KEGGecj:Y75_p1678.
eco:b1703.
PATRIC32118712. VBIEscCol129921_1774.

Organism-specific databases

EchoBASEEB1122.
EcoGeneEG11132. ppsR.

Phylogenomic databases

eggNOGCOG1806.
HOGENOMHOG000218053.
KOK09773.
OMATTNYSVE.
ProtClustDBPRK05339.

Enzyme and pathway databases

BioCycEcoCyc:EG11132-MONOMER.
ECOL316407:JW1693-MONOMER.

Gene expression databases

GenevestigatorP0A8A4.

Family and domain databases

HAMAPMF_01062. PSRP.
InterProIPR005177. Kinase-pyrophosphorylase.
IPR026530. PSRP.
[Graphical view]
PfamPF03618. Kinase-PPPase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSRP_ECOLI
AccessionPrimary (citable) accession number: P0A8A4
Secondary accession number(s): P03822, P46137, P76203
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families