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Protein

Phosphoenolpyruvate synthase regulatory protein

Gene

ppsR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the phosphoenolpyruvate synthase (PEPS) by catalyzing its phosphorylation/dephosphorylation.UniRule annotation1 Publication

Catalytic activityi

ADP + [pyruvate, water dikinase] = AMP + [pyruvate, water dikinase] phosphate.UniRule annotation1 Publication
[Pyruvate, water dikinase] phosphate + phosphate = [pyruvate, water dikinase] + diphosphate.UniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi157 – 164ADPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11132-MONOMER.
ECOL316407:JW1693-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate synthase regulatory proteinUniRule annotation (EC:2.7.11.33UniRule annotation, EC:2.7.4.28UniRule annotation)
Short name:
PEP synthase regulatory proteinUniRule annotation
Short name:
PSRPUniRule annotation
Alternative name(s):
Pyruvate, water dikinase regulatory proteinUniRule annotation
Gene namesi
Name:ppsRUniRule annotation
Synonyms:ydiA
Ordered Locus Names:b1703, JW1693
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11132. ppsR.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001966591 – 277Phosphoenolpyruvate synthase regulatory proteinAdd BLAST277

Proteomic databases

EPDiP0A8A4.
PaxDbiP0A8A4.
PRIDEiP0A8A4.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rluDP336432EBI-548302,EBI-558026

Protein-protein interaction databases

BioGridi4260288. 13 interactors.
DIPiDIP-47885N.
IntActiP0A8A4. 13 interactors.
MINTiMINT-1236315.
STRINGi511145.b1703.

Structurei

3D structure databases

ProteinModelPortaliP0A8A4.
SMRiP0A8A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PSRP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CF9. Bacteria.
COG1806. LUCA.
HOGENOMiHOG000218053.
InParanoidiP0A8A4.
KOiK09773.
OMAiYAQCEFE.
PhylomeDBiP0A8A4.

Family and domain databases

HAMAPiMF_01062. PSRP. 1 hit.
InterProiIPR005177. Kinase-pyrophosphorylase.
IPR026530. PSRP.
[Graphical view]
PfamiPF03618. Kinase-PPPase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A8A4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNAVDRHVF YISDGTAITA EVLGHAVMSQ FPVTISSITL PFVENESRAR
60 70 80 90 100
AVKDQIDAIY HQTGVRPLVF YSIVLPEIRA IILQSEGFCQ DIVQALVAPL
110 120 130 140 150
QQEMKLDPTP IAHRTHGLNP NNLNKYDARI AAIDYTLAHD DGISLRNLDQ
160 170 180 190 200
AQVILLGVSR CGKTPTSLYL AMQFGIRAAN YPFIADDMDN LVLPASLKPL
210 220 230 240 250
QHKLFGLTID PERLAAIREE RRENSRYASL RQCRMEVAEV EALYRKNQIP
260 270
WINSTNYSVE EIATKILDIM GLSRRMY
Length:277
Mass (Da):31,211
Last modified:June 7, 2005 - v1
Checksum:i25508DEEC2E342CA
GO

Sequence cautioni

The sequence M69116 differs from that shown. Reason: Frameshift at positions 64 and 90.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74773.1.
AP009048 Genomic DNA. Translation: BAA15472.2.
M69116 Genomic DNA. No translation available.
M38266 Genomic DNA. Translation: AAA23496.1.
V00261 Genomic DNA. No translation available.
PIRiG64928. QQECAD.
RefSeqiNP_416218.1. NC_000913.3.
WP_000368046.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74773; AAC74773; b1703.
BAA15472; BAA15472; BAA15472.
GeneIDi946207.
KEGGiecj:JW1693.
eco:b1703.
PATRICi32118712. VBIEscCol129921_1774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74773.1.
AP009048 Genomic DNA. Translation: BAA15472.2.
M69116 Genomic DNA. No translation available.
M38266 Genomic DNA. Translation: AAA23496.1.
V00261 Genomic DNA. No translation available.
PIRiG64928. QQECAD.
RefSeqiNP_416218.1. NC_000913.3.
WP_000368046.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A8A4.
SMRiP0A8A4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260288. 13 interactors.
DIPiDIP-47885N.
IntActiP0A8A4. 13 interactors.
MINTiMINT-1236315.
STRINGi511145.b1703.

Proteomic databases

EPDiP0A8A4.
PaxDbiP0A8A4.
PRIDEiP0A8A4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74773; AAC74773; b1703.
BAA15472; BAA15472; BAA15472.
GeneIDi946207.
KEGGiecj:JW1693.
eco:b1703.
PATRICi32118712. VBIEscCol129921_1774.

Organism-specific databases

EchoBASEiEB1122.
EcoGeneiEG11132. ppsR.

Phylogenomic databases

eggNOGiENOG4105CF9. Bacteria.
COG1806. LUCA.
HOGENOMiHOG000218053.
InParanoidiP0A8A4.
KOiK09773.
OMAiYAQCEFE.
PhylomeDBiP0A8A4.

Enzyme and pathway databases

BioCyciEcoCyc:EG11132-MONOMER.
ECOL316407:JW1693-MONOMER.

Miscellaneous databases

PROiP0A8A4.

Family and domain databases

HAMAPiMF_01062. PSRP. 1 hit.
InterProiIPR005177. Kinase-pyrophosphorylase.
IPR026530. PSRP.
[Graphical view]
PfamiPF03618. Kinase-PPPase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSRP_ECOLI
AccessioniPrimary (citable) accession number: P0A8A4
Secondary accession number(s): P03822, P46137, P76203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.