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Protein

RNase adapter protein RapZ

Gene

rapZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates the synthesis of GlmS, by affecting the processing and stability of the regulatory small RNA GlmZ. When glucosamine-6-phosphate (GlcN6P) concentrations are high in the cell, RapZ binds GlmZ and targets it to cleavage by RNase E. Consequently, GlmZ is inactivated and unable to activate GlmS synthesis. Under low GlcN6P concentrations, RapZ is sequestered and inactivated by an other regulatory small RNA, GlmY, preventing GlmZ degradation and leading to synthesis of GlmS (PubMed:17824929, PubMed:23475961). Displays ATPase and GTPase activities in vitro. Can also hydrolyze pNPP (PubMed:19074378).3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPUniRule annotation
Nucleotide bindingi56 – 594GTPUniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • GTP binding Source: UniProtKB-HAMAP
  • identical protein binding Source: EcoCyc
  • RNA binding Source: EcoCyc

GO - Biological processi

  • positive regulation of biological process Source: EcoCyc
  • RNA processing Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12146-MONOMER.
ECOL316407:JW3172-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNase adapter protein RapZ1 PublicationUniRule annotation
Gene namesi
Name:rapZ1 PublicationUniRule annotation
Synonyms:yhbJ
Ordered Locus Names:b3205, JW3172
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12146. yhbJ.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene strongly overproduce the GlmS protein.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi270 – 2701K → A: Lack of activity. Does not bind GlmY and GlmZ; when associated with A-281; A-282 and A-283. 1 Publication
Mutagenesisi281 – 2811K → A: Lack of activity. Does not bind GlmY and GlmZ; when associated with A-270; A-282 and A-283. 1 Publication
Mutagenesisi282 – 2821R → A: Lack of activity. Does not bind GlmY and GlmZ; when associated with A-270; A-281 and A-283. 1 Publication
Mutagenesisi283 – 2831K → A: Lack of activity. Does not bind GlmY and GlmZ; when associated with A-270; A-281 and A-282. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284RNase adapter protein RapZPRO_0000107705Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei251 – 2511N6-acetyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0A894.
PaxDbiP0A894.
PRIDEiP0A894.

Interactioni

Subunit structurei

Homotrimer (PubMed:23385747). Interacts with the small RNAs GlmY and GlmZ (PubMed:23475961). Interacts with the catalytic domain of RNase E in an RNA-independent manner (PubMed:23475961).2 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259285. 7 interactions.
DIPiDIP-35927N.
IntActiP0A894. 15 interactions.
MINTiMINT-1232648.
STRINGi511145.b3205.

Structurei

3D structure databases

ProteinModelPortaliP0A894.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 28419RNA-bindingUniRule annotation1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the RapZ-like family. RapZ subfamily.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C21. Bacteria.
COG1660. LUCA.
HOGENOMiHOG000244885.
InParanoidiP0A894.
KOiK06958.
OMAiVSFGYKY.
PhylomeDBiP0A894.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00636. RapZ_like. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005337. RapZ-like.
[Graphical view]
PfamiPF03668. ATP_bind_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005052. P-loopkin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLMIVSGRS GSGKSVALRA LEDMGFYCVD NLPVVLLPDL ARTLADREIS
60 70 80 90 100
AAVSIDVRNM PESPEIFEQA MSNLPDAFSP QLLFLDADRN TLIRRYSDTR
110 120 130 140 150
RLHPLSSKNL SLESAIDKES DLLEPLRSRA DLIVDTSEMS VHELAEMLRT
160 170 180 190 200
RLLGKREREL TMVFESFGFK HGIPIDADYV FDVRFLPNPH WDPKLRPMTG
210 220 230 240 250
LDKPVAAFLD RHTEVHNFIY QTRSYLELWL PMLETNNRSY LTVAIGCTGG
260 270 280
KHRSVYIAEQ LADYFRSRGK NVQSRHRTLE KRKP
Length:284
Mass (Da):32,492
Last modified:June 7, 2005 - v1
Checksum:iBB1525B1E044126C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27094 Genomic DNA. Translation: CAA81620.1.
U12684 Genomic DNA. Translation: AAB60166.1.
U18997 Genomic DNA. Translation: AAA58007.1.
U00096 Genomic DNA. Translation: AAC76237.1.
AP009048 Genomic DNA. Translation: BAE77249.1.
PIRiI76721.
RefSeqiNP_417672.1. NC_000913.3.
WP_000243741.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76237; AAC76237; b3205.
BAE77249; BAE77249; BAE77249.
GeneIDi947727.
KEGGiecj:JW3172.
eco:b3205.
PATRICi32121830. VBIEscCol129921_3299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z27094 Genomic DNA. Translation: CAA81620.1.
U12684 Genomic DNA. Translation: AAB60166.1.
U18997 Genomic DNA. Translation: AAA58007.1.
U00096 Genomic DNA. Translation: AAC76237.1.
AP009048 Genomic DNA. Translation: BAE77249.1.
PIRiI76721.
RefSeqiNP_417672.1. NC_000913.3.
WP_000243741.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A894.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259285. 7 interactions.
DIPiDIP-35927N.
IntActiP0A894. 15 interactions.
MINTiMINT-1232648.
STRINGi511145.b3205.

Proteomic databases

EPDiP0A894.
PaxDbiP0A894.
PRIDEiP0A894.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76237; AAC76237; b3205.
BAE77249; BAE77249; BAE77249.
GeneIDi947727.
KEGGiecj:JW3172.
eco:b3205.
PATRICi32121830. VBIEscCol129921_3299.

Organism-specific databases

EchoBASEiEB2066.
EcoGeneiEG12146. yhbJ.

Phylogenomic databases

eggNOGiENOG4105C21. Bacteria.
COG1660. LUCA.
HOGENOMiHOG000244885.
InParanoidiP0A894.
KOiK06958.
OMAiVSFGYKY.
PhylomeDBiP0A894.

Enzyme and pathway databases

BioCyciEcoCyc:EG12146-MONOMER.
ECOL316407:JW3172-MONOMER.

Miscellaneous databases

PROiP0A894.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00636. RapZ_like. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005337. RapZ-like.
[Graphical view]
PfamiPF03668. ATP_bind_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005052. P-loopkin. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRAPZ_ECOLI
AccessioniPrimary (citable) accession number: P0A894
Secondary accession number(s): P33995, Q2M907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.