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Protein

Sulfur carrier protein TusA

Gene

tusA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfur carrier protein involved in sulfur trafficking in the cell. Part of a sulfur-relay system required for 2-thiolation during synthesis of 2-thiouridine of the modified wobble base 5-methylaminomethyl-2-thiouridine (mnm5s2U) in tRNA (PubMed:16387657). Interacts with IscS and stimulates its cysteine desulfurase activity (PubMed:16387657, PubMed:23281480). Accepts an activated sulfur from IscS, which is then transferred to TusD, and thus determines the direction of sulfur flow from IscS to 2-thiouridine formation (PubMed:16387657). Also appears to be involved in sulfur transfer for the biosynthesis of molybdopterin (PubMed:23281480). Seems to affect the stability of sigma-S, particularly during the logarithmic growth phase (PubMed:9555915).3 Publications

Pathwayi: tRNA modification

This protein is involved in tRNA modification.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei19Cysteine persulfide intermediateUniRule annotation1 Publication1

GO - Molecular functioni

  • sulfur carrier activity Source: EcoCyc
  • sulfurtransferase activity Source: InterPro

GO - Biological processi

  • Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
  • tRNA wobble position uridine thiolation Source: EcoCyc

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12216-MONOMER.
MetaCyc:EG12216-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfur carrier protein TusA1 PublicationUniRule annotation
Alternative name(s):
Sulfur mediator TusA1 PublicationUniRule annotation
Sulfur transfer protein TusA1 PublicationUniRule annotation
tRNA 2-thiouridine synthesizing protein A1 PublicationUniRule annotation
Gene namesi
Name:tusA1 PublicationUniRule annotation
Synonyms:sirA, yhhP
Ordered Locus Names:b3470, JW3435
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12216. tusA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene are not capable of growing in standard laboratory rich medium (i.e., Luria broth), and show filamentous shape (PubMed:9555915). FtsZ-ring formation appears to be severely impaired in tusA-deficient cells, resulting in the formation of a non-divided filamentous cell (PubMed:10830496). A tusA deletion mutant lacks the 2-thio modification of mnm5s2U in tRNA and has a severe growth defect (PubMed:16387657). Deletion of tusA has a pleiotropic effect on transcription, including increased expression of molybdenum cofactor biosynthesis genes (moaABCDE operon), but leads to reduced activity of molybdoenzymes, an overall low molybdopterin content and an accumulation of the molybdopterin precursor cyclic pyranopterin monophopshate (cPMP) (PubMed:23281480).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19C → S: Unable to restore mnm5s2U formation in a tusA-deficient mutant. Unable to accept sulfur from IscS. 1 Publication1
Mutagenesisi21E → K in sirA1; affects the stability of sigma-S during the logarithmic growth phase. 1 Publication1
Mutagenesisi56C → S: Able to restore mnm5s2U formation in a tusA-deficient mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001590331 – 81Sulfur carrier protein TusAAdd BLAST81

Proteomic databases

PaxDbiP0A890.
PRIDEiP0A890.

Interactioni

Subunit structurei

Interacts with IscS.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
iscSP0A6B75EBI-561780,EBI-550055

Protein-protein interaction databases

DIPiDIP-48202N.
IntActiP0A890. 16 interactors.
STRINGi511145.b3470.

Structurei

Secondary structure

181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 12Combined sources4
Helixi21 – 32Combined sources12
Beta strandi39 – 43Combined sources5
Helixi48 – 58Combined sources11
Beta strandi62 – 67Combined sources6
Beta strandi69 – 78Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCJNMR-A1-81[»]
ProteinModelPortaliP0A890.
SMRiP0A890.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A890.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfur carrier protein TusA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41082K8. Bacteria.
COG0425. LUCA.
HOGENOMiHOG000259291.
InParanoidiP0A890.
KOiK04085.
OMAiVRQMETG.
PhylomeDBiP0A890.

Family and domain databases

CDDicd03423. SirA. 1 hit.
Gene3Di3.30.110.40. 1 hit.
HAMAPiMF_00413. Thiourid_synth_A. 1 hit.
InterProiView protein in InterPro
IPR022931. Sulphurtransferase_TusA.
IPR001455. TusA-like.
PfamiView protein in Pfam
PF01206. TusA. 1 hit.
SUPFAMiSSF64307. SSF64307. 1 hit.
PROSITEiView protein in PROSITE
PS01148. UPF0033. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLFSSPDH TLDALGLRCP EPVMMVRKTV RNMQPGETLL IIADDPATTR
60 70 80
DIPGFCTFME HELVAKETDG LPYRYLIRKG G
Length:81
Mass (Da):9,095
Last modified:June 7, 2005 - v1
Checksum:i63AA7DE4816A3274
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18445.1.
U00096 Genomic DNA. Translation: AAC76495.1.
AP009048 Genomic DNA. Translation: BAE77823.1.
PIRiS47689.
RefSeqiNP_417927.1. NC_000913.3.
WP_000130621.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76495; AAC76495; b3470.
BAE77823; BAE77823; BAE77823.
GeneIDi947974.
KEGGiecj:JW3435.
eco:b3470.
PATRICi32122384. VBIEscCol129921_3569.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18445.1.
U00096 Genomic DNA. Translation: AAC76495.1.
AP009048 Genomic DNA. Translation: BAE77823.1.
PIRiS47689.
RefSeqiNP_417927.1. NC_000913.3.
WP_000130621.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCJNMR-A1-81[»]
ProteinModelPortaliP0A890.
SMRiP0A890.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48202N.
IntActiP0A890. 16 interactors.
STRINGi511145.b3470.

Proteomic databases

PaxDbiP0A890.
PRIDEiP0A890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76495; AAC76495; b3470.
BAE77823; BAE77823; BAE77823.
GeneIDi947974.
KEGGiecj:JW3435.
eco:b3470.
PATRICi32122384. VBIEscCol129921_3569.

Organism-specific databases

EchoBASEiEB2130.
EcoGeneiEG12216. tusA.

Phylogenomic databases

eggNOGiENOG41082K8. Bacteria.
COG0425. LUCA.
HOGENOMiHOG000259291.
InParanoidiP0A890.
KOiK04085.
OMAiVRQMETG.
PhylomeDBiP0A890.

Enzyme and pathway databases

BioCyciEcoCyc:EG12216-MONOMER.
MetaCyc:EG12216-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A890.
PROiPR:P0A890.

Family and domain databases

CDDicd03423. SirA. 1 hit.
Gene3Di3.30.110.40. 1 hit.
HAMAPiMF_00413. Thiourid_synth_A. 1 hit.
InterProiView protein in InterPro
IPR022931. Sulphurtransferase_TusA.
IPR001455. TusA-like.
PfamiView protein in Pfam
PF01206. TusA. 1 hit.
SUPFAMiSSF64307. SSF64307. 1 hit.
PROSITEiView protein in PROSITE
PS01148. UPF0033. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTUSA_ECOLI
AccessioniPrimary (citable) accession number: P0A890
Secondary accession number(s): P37618, Q2M7D3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 15, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families
  5. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.