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Protein

Sulfurtransferase TusA

Gene

tusA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at tRNA wobble positions. Interacts with IscS and stimulates its activity. Then, accepts a sulfur from IscS and transfers it in turn to TusD. Seems to affect the stability of sigma-S, particularly during the logarithmic growth phase.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191Cysteine persulfide intermediate

GO - Molecular functioni

  • sulfur carrier activity Source: EcoCyc
  • sulfurtransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
  • tRNA wobble position uridine thiolation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG12216-MONOMER.
ECOL316407:JW3435-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfurtransferase TusA (EC:2.8.1.-)
Alternative name(s):
tRNA 2-thiouridine synthesizing protein A
Gene namesi
Name:tusA
Synonyms:sirA, yhhP
Ordered Locus Names:b3470, JW3435
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12216. tusA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191C → S: Loss of activity. 1 Publication
Mutagenesisi21 – 211E → K in sirA1; affects the stability of sigma-S during the logarithmic growth phase. 1 Publication
Mutagenesisi56 – 561C → S: No effect. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8181Sulfurtransferase TusAPRO_0000159033Add
BLAST

Proteomic databases

EPDiP0A890.
PaxDbiP0A890.
PRIDEiP0A890.

Interactioni

Subunit structurei

Interacts with IscS.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
iscSP0A6B75EBI-561780,EBI-550055

Protein-protein interaction databases

DIPiDIP-48202N.
IntActiP0A890. 16 interactions.
STRINGi511145.b3470.

Structurei

Secondary structure

1
81
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 124Combined sources
Helixi21 – 3212Combined sources
Beta strandi39 – 435Combined sources
Helixi48 – 5811Combined sources
Beta strandi62 – 676Combined sources
Beta strandi69 – 7810Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCJNMR-A1-81[»]
ProteinModelPortaliP0A890.
SMRiP0A890. Positions 1-81.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A890.

Family & Domainsi

Sequence similaritiesi

Belongs to the UPF0033 family. TusA subfamily.Curated

Phylogenomic databases

eggNOGiENOG41082K8. Bacteria.
COG0425. LUCA.
HOGENOMiHOG000259291.
InParanoidiP0A890.
KOiK04085.
OMAiVRQMETG.
OrthoDBiEOG6QCD6D.
PhylomeDBiP0A890.

Family and domain databases

Gene3Di3.30.110.40. 1 hit.
HAMAPiMF_00413. Thiourid_synth_A.
InterProiIPR022931. Sulphurtransferase_TusA.
IPR001455. TusA-like.
[Graphical view]
PfamiPF01206. TusA. 1 hit.
[Graphical view]
SUPFAMiSSF64307. SSF64307. 1 hit.
PROSITEiPS01148. UPF0033. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDLFSSPDH TLDALGLRCP EPVMMVRKTV RNMQPGETLL IIADDPATTR
60 70 80
DIPGFCTFME HELVAKETDG LPYRYLIRKG G
Length:81
Mass (Da):9,095
Last modified:June 7, 2005 - v1
Checksum:i63AA7DE4816A3274
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18445.1.
U00096 Genomic DNA. Translation: AAC76495.1.
AP009048 Genomic DNA. Translation: BAE77823.1.
PIRiS47689.
RefSeqiNP_417927.1. NC_000913.3.
WP_000130621.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76495; AAC76495; b3470.
BAE77823; BAE77823; BAE77823.
GeneIDi947974.
KEGGiecj:JW3435.
eco:b3470.
PATRICi32122384. VBIEscCol129921_3569.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00039 Genomic DNA. Translation: AAB18445.1.
U00096 Genomic DNA. Translation: AAC76495.1.
AP009048 Genomic DNA. Translation: BAE77823.1.
PIRiS47689.
RefSeqiNP_417927.1. NC_000913.3.
WP_000130621.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DCJNMR-A1-81[»]
ProteinModelPortaliP0A890.
SMRiP0A890. Positions 1-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48202N.
IntActiP0A890. 16 interactions.
STRINGi511145.b3470.

Proteomic databases

EPDiP0A890.
PaxDbiP0A890.
PRIDEiP0A890.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76495; AAC76495; b3470.
BAE77823; BAE77823; BAE77823.
GeneIDi947974.
KEGGiecj:JW3435.
eco:b3470.
PATRICi32122384. VBIEscCol129921_3569.

Organism-specific databases

EchoBASEiEB2130.
EcoGeneiEG12216. tusA.

Phylogenomic databases

eggNOGiENOG41082K8. Bacteria.
COG0425. LUCA.
HOGENOMiHOG000259291.
InParanoidiP0A890.
KOiK04085.
OMAiVRQMETG.
OrthoDBiEOG6QCD6D.
PhylomeDBiP0A890.

Enzyme and pathway databases

BioCyciEcoCyc:EG12216-MONOMER.
ECOL316407:JW3435-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A890.
PROiP0A890.

Family and domain databases

Gene3Di3.30.110.40. 1 hit.
HAMAPiMF_00413. Thiourid_synth_A.
InterProiIPR022931. Sulphurtransferase_TusA.
IPR001455. TusA-like.
[Graphical view]
PfamiPF01206. TusA. 1 hit.
[Graphical view]
SUPFAMiSSF64307. SSF64307. 1 hit.
PROSITEiPS01148. UPF0033. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
    Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
    Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The yhhP gene encoding a small ubiquitous protein is fundamental for normal cell growth of Escherichia coli."
    Yamashino T., Isomura M., Ueguchi C., Mizuno T.
    J. Bacteriol. 180:2257-2261(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLU-21.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  5. "Deletion of the yhhP gene results in filamentous cell morphology in Escherichia coli."
    Ishii Y., Yamada H., Yamashino T., Ohashi K., Katoh E., Shindo H., Yamazaki T., Mizuno T.
    Biosci. Biotechnol. Biochem. 64:799-807(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions."
    Ikeuchi Y., Shigi N., Kato J., Nishimura A., Suzuki T.
    Mol. Cell 21:97-108(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ISCS, IDENTIFICATION BY MASS SPECTROMETRY, MUTAGENESIS OF CYS-19 AND CYS-56.
  7. "High precision NMR structure of YhhP, a novel Escherichia coli protein implicated in cell division."
    Katoh E., Hatta T., Shindo H., Ishii Y., Yamada H., Mizuno T., Yamazaki T.
    J. Mol. Biol. 304:219-229(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiTUSA_ECOLI
AccessioniPrimary (citable) accession number: P0A890
Secondary accession number(s): P37618, Q2M7D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 6, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.