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Protein

Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE

Gene

ubiE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).UniRule annotation1 Publication

Catalytic activityi

A demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine.UniRule annotation1 Publication
S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol.UniRule annotation1 Publication

Pathwayi: menaquinone biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
  2. Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)
This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
Binding sitei140 – 1401S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  • 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity Source: UniProtKB-EC
  • 2-octaprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Source: EcoCyc
  • S-adenosylmethionine-dependent methyltransferase activity Source: EcoCyc

GO - Biological processi

  • aerobic respiration Source: UniProtKB-HAMAP
  • menaquinone biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Menaquinone biosynthesis, Ubiquinone biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
ECOL316407:JW5581-MONOMER.
MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
BRENDAi2.1.1.163. 2026.
UniPathwayiUPA00079; UER00169.
UPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEUniRule annotation (EC:2.1.1.163UniRule annotation1 Publication, EC:2.1.1.201UniRule annotation1 Publication)
Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylaseUniRule annotation
Demethylmenaquinone methyltransferaseUniRule annotation
Gene namesi
Name:ubiE1 PublicationUniRule annotation
Synonyms:yigO
Ordered Locus Names:b3833, JW5581
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11473. ubiE.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi142 – 1421G → D in ubiE401; defective. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEPRO_0000193274Add
BLAST

Proteomic databases

EPDiP0A887.
PaxDbiP0A887.
PRIDEiP0A887.

Interactioni

Protein-protein interaction databases

BioGridi4261082. 513 interactions.
DIPiDIP-31831N.
IntActiP0A887. 6 interactions.
MINTiMINT-1243319.
STRINGi511145.b3833.

Structurei

3D structure databases

ProteinModelPortaliP0A887.
SMRiP0A887. Positions 31-250.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DDZ. Bacteria.
COG2226. LUCA.
HOGENOMiHOG000249463.
InParanoidiP0A887.
KOiK03183.
OMAiGGLHRAW.
OrthoDBiEOG6M6JSB.
PhylomeDBiP0A887.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01813. MenG_UbiE_methyltr.
InterProiIPR029063. SAM-dependent_MTases.
IPR004033. UbiE/COQ5_MeTrFase.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PfamiPF01209. Ubie_methyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITEiPS51608. SAM_MT_UBIE. 1 hit.
PS01183. UBIE_1. 1 hit.
PS01184. UBIE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL
60 70 80 90 100
WKRFTIDCSG VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM
110 120 130 140 150
LKMGREKLRN IGVIGNVEYV QANAEALPFP DNTFDCITIS FGLRNVTDKD
160 170 180 190 200
KALRSMYRVL KPGGRLLVLE FSKPIIEPLS KAYDAYSFHV LPRIGSLVAN
210 220 230 240 250
DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA GVVALHRGYK

F
Length:251
Mass (Da):28,073
Last modified:June 7, 2005 - v1
Checksum:iB1EEE209BDC68F5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67628.1.
U00096 Genomic DNA. Translation: AAT48227.1.
AP009048 Genomic DNA. Translation: BAE77468.1.
PIRiB65188.
RefSeqiWP_000227958.1. NZ_LN832404.1.
YP_026269.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48227; AAT48227; b3833.
BAE77468; BAE77468; BAE77468.
GeneIDi948926.
KEGGiecj:JW5581.
eco:b3833.
PATRICi32123167. VBIEscCol129921_3949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67628.1.
U00096 Genomic DNA. Translation: AAT48227.1.
AP009048 Genomic DNA. Translation: BAE77468.1.
PIRiB65188.
RefSeqiWP_000227958.1. NZ_LN832404.1.
YP_026269.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0A887.
SMRiP0A887. Positions 31-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261082. 513 interactions.
DIPiDIP-31831N.
IntActiP0A887. 6 interactions.
MINTiMINT-1243319.
STRINGi511145.b3833.

Proteomic databases

EPDiP0A887.
PaxDbiP0A887.
PRIDEiP0A887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48227; AAT48227; b3833.
BAE77468; BAE77468; BAE77468.
GeneIDi948926.
KEGGiecj:JW5581.
eco:b3833.
PATRICi32123167. VBIEscCol129921_3949.

Organism-specific databases

EchoBASEiEB1441.
EcoGeneiEG11473. ubiE.

Phylogenomic databases

eggNOGiENOG4105DDZ. Bacteria.
COG2226. LUCA.
HOGENOMiHOG000249463.
InParanoidiP0A887.
KOiK03183.
OMAiGGLHRAW.
OrthoDBiEOG6M6JSB.
PhylomeDBiP0A887.

Enzyme and pathway databases

UniPathwayiUPA00079; UER00169.
UPA00232.
BioCyciEcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
ECOL316407:JW5581-MONOMER.
MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
BRENDAi2.1.1.163. 2026.

Miscellaneous databases

PROiP0A887.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01813. MenG_UbiE_methyltr.
InterProiIPR029063. SAM-dependent_MTases.
IPR004033. UbiE/COQ5_MeTrFase.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PfamiPF01209. Ubie_methyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITEiPS51608. SAM_MT_UBIE. 1 hit.
PS01183. UBIE_1. 1 hit.
PS01184. UBIE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: SEQUENCE REVISION TO 101.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene."
    Lee P.T., Hsu A.Y., Ha H.T., Clarke C.F.
    J. Bacteriol. 179:1748-1754(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY, MUTANT UBIE401.

Entry informationi

Entry nameiUBIE_ECOLI
AccessioniPrimary (citable) accession number: P0A887
Secondary accession number(s): P27851, Q2M8D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: May 11, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.