Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE

Gene

ubiE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).UniRule annotation1 Publication

Catalytic activityi

A demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine.UniRule annotation1 Publication
S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol.UniRule annotation1 Publication

Pathwayi: menaquinone biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
  2. Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)
This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74S-adenosyl-L-methionineUniRule annotation1
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Binding sitei140S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • menaquinone biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processMenaquinone biosynthesis, Ubiquinone biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER
MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER
BRENDAi2.1.1.163 2026
UniPathwayiUPA00079; UER00169
UPA00232

Chemistry databases

SwissLipidsiSLP:000001505

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEUniRule annotation (EC:2.1.1.163UniRule annotation1 Publication, EC:2.1.1.201UniRule annotation1 Publication)
Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylaseUniRule annotation
Demethylmenaquinone methyltransferaseUniRule annotation
Gene namesi
Name:ubiE1 PublicationUniRule annotation
Synonyms:yigO
Ordered Locus Names:b3833, JW5581
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11473 ubiE

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi142G → D in ubiE401; defective. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932741 – 251Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEAdd BLAST251

Proteomic databases

EPDiP0A887
PaxDbiP0A887
PRIDEiP0A887

Interactioni

Protein-protein interaction databases

BioGridi4261082536 interactors.
DIPiDIP-31831N
IntActiP0A887 6 interactors.
STRINGi316385.ECDH10B_4024

Structurei

3D structure databases

ProteinModelPortaliP0A887
SMRiP0A887
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DDZ Bacteria
COG2226 LUCA
HOGENOMiHOG000249463
InParanoidiP0A887
KOiK03183
OMAiVRNFENL
PhylomeDBiP0A887

Family and domain databases

HAMAPiMF_01813 MenG_UbiE_methyltr, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR004033 UbiE/COQ5_MeTrFase
IPR023576 UbiE/COQ5_MeTrFase_CS
PfamiView protein in Pfam
PF01209 Ubie_methyltran, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR01934 MenG_MenH_UbiE, 1 hit
PROSITEiView protein in PROSITE
PS51608 SAM_MT_UBIE, 1 hit
PS01183 UBIE_1, 1 hit
PS01184 UBIE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL
60 70 80 90 100
WKRFTIDCSG VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM
110 120 130 140 150
LKMGREKLRN IGVIGNVEYV QANAEALPFP DNTFDCITIS FGLRNVTDKD
160 170 180 190 200
KALRSMYRVL KPGGRLLVLE FSKPIIEPLS KAYDAYSFHV LPRIGSLVAN
210 220 230 240 250
DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA GVVALHRGYK

F
Length:251
Mass (Da):28,073
Last modified:June 7, 2005 - v1
Checksum:iB1EEE209BDC68F5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA Translation: AAA67628.1
U00096 Genomic DNA Translation: AAT48227.1
AP009048 Genomic DNA Translation: BAE77468.1
PIRiB65188
RefSeqiWP_000227958.1, NZ_LN832404.1
YP_026269.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48227; AAT48227; b3833
BAE77468; BAE77468; BAE77468
GeneIDi948926
KEGGiecj:JW5581
eco:b3833
PATRICifig|1411691.4.peg.2875

Similar proteinsi

Entry informationi

Entry nameiUBIE_ECOLI
AccessioniPrimary (citable) accession number: P0A887
Secondary accession number(s): P27851, Q2M8D8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome