P0A887 (UBIE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinone/menaquinone biosynthesis methyltransferase ubiE
EC=2.1.1.163
EC=2.1.1.201

Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
Demethylmenaquinone methyltransferase

Gene names

Name:	ubiE
Synonyms:	yigO
Ordered Locus Names:	b3833, JW5581

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2). HAMAP-Rule MF_01813
Catalytic activity	A demethylmenaquinone + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine. Ref.5 S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol. Ref.5
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 8/8. HAMAP-Rule MF_01813 Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP-Rule MF_01813
Sequence similarities	Belongs to the methyltransferase superfamily. UbiE family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis Ubiquinone biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from electronic annotation. Source: HAMAP menaquinone biosynthetic process Inferred from mutant phenotype PubMed 1444716. Source: EcoCyc ubiquinone biosynthetic process Inferred from mutant phenotype PubMed 4323297. Source: EcoCyc
Molecular_function	2-octaprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from mutant phenotype PubMed 1444716. Source: EcoCyc S-adenosylmethionine-dependent methyltransferase activity Inferred from sequence or structural similarity Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Ubiquinone/menaquinone biosynthesis methyltransferase ubiE HAMAP-Rule MF_01813		PRO_0000193274
Experimental info
	Mutagenesis	142	1	G → D in ubiE401; defective.

Sequences

	Sequence		Length	Mass (Da)
	P0A887 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: B1EEE209BDC68F5E	FASTA	251	28,073
	10 20 30 40 50 60 MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL WKRFTIDCSG 70 80 90 100 110 120 VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM LKMGREKLRN IGVIGNVEYV 130 140 150 160 170 180 QANAEALPFP DNTFDCITIS FGLRNVTDKD KALRSMYRVL KPGGRLLVLE FSKPIIEPLS 190 200 210 220 230 240 KAYDAYSFHV LPRIGSLVAN DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA 250 GVVALHRGYK F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005." Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L. Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 101.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene." Lee P.T., Hsu A.Y., Ha H.T., Clarke C.F. J. Bacteriol. 179:1748-1754(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, CHARACTERIZATION, MUTANT UBIE401.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M87049 Genomic DNA. Translation: AAA67628.1. U00096 Genomic DNA. Translation: AAT48227.1. AP009048 Genomic DNA. Translation: BAE77468.1.
PIR	B65188.
RefSeq	YP_026269.1. NC_000913.2. YP_491609.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A887.
SMR	P0A887. Positions 29-231.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31831N.
IntAct	P0A887. 4 interactions.
MINT	MINT-1243319.
STRING	511145.b3833.
Proteomic databases
PaxDb	P0A887.
PRIDE	P0A887.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT48227; AAT48227; b3833. BAE77468; BAE77468; BAE77468.
GeneID	12932088. 948926.
KEGG	ecj:Y75_p3345. eco:b3833.
PATRIC	32123167. VBIEscCol129921_3949.
Organism-specific databases
EchoBASE	EB1441.
EcoGene	EG11473. ubiE.
Phylogenomic databases
eggNOG	COG2226.
HOGENOM	HOG000249463.
KO	K03183.
OMA	PYQYLVE.
ProtClustDB	PRK00216.
Enzyme and pathway databases
BioCyc	EcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER. ECOL316407:JW5581-MONOMER. MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
UniPathway	UPA00079; UER00169. UPA00232.
Gene expression databases
Genevestigator	P0A887.
Family and domain databases
HAMAP	MF_01813. UbiE_methyltran.
InterPro	IPR004033. UbiE/COQ5_MeTrFase. IPR023576. UbiE/COQ5_MeTrFase_CS. IPR025765. UbiE/COQ5_MeTrFase_dom. [Graphical view]
PANTHER	PTHR10108:SF24. PTHR10108:SF24. 1 hit.
Pfam	PF01209. Ubie_methyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITE	PS01183. UBIE_1. 1 hit. PS01184. UBIE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIE_ECOLI

Accession

Primary (citable) accession number: P0A887
Secondary accession number(s): P27851, Q2M8D8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program