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Protein

Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE

Gene

ubiE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Methyltransferase required for the conversion of demethylmenaquinol (DMKH2) to menaquinol (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).UniRule annotation1 Publication

Catalytic activityi

A demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine.UniRule annotation1 Publication
S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol.UniRule annotation1 Publication

Pathwayi: menaquinone biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate.UniRule annotation1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1,4-dihydroxy-2-naphthoate octaprenyltransferase (menA)
  2. Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE (ubiE)
This subpathway is part of the pathway menaquinone biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes menaquinol from 1,4-dihydroxy-2-naphthoate, the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74S-adenosyl-L-methionineUniRule annotation1
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Binding sitei140S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase activity Source: UniProtKB-EC
  • 2-octaprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Source: EcoCyc
  • S-adenosylmethionine-dependent methyltransferase activity Source: EcoCyc

GO - Biological processi

  • aerobic respiration Source: UniProtKB-HAMAP
  • menaquinone biosynthetic process Source: EcoCyc
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Menaquinone biosynthesis, Ubiquinone biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
ECOL316407:JW5581-MONOMER.
MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
BRENDAi2.1.1.163. 2026.
UniPathwayiUPA00079; UER00169.
UPA00232.

Chemistry databases

SwissLipidsiSLP:000001505.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEUniRule annotation (EC:2.1.1.163UniRule annotation1 Publication, EC:2.1.1.201UniRule annotation1 Publication)
Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylaseUniRule annotation
Demethylmenaquinone methyltransferaseUniRule annotation
Gene namesi
Name:ubiE1 PublicationUniRule annotation
Synonyms:yigO
Ordered Locus Names:b3833, JW5581
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11473. ubiE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi142G → D in ubiE401; defective. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932741 – 251Ubiquinone/menaquinone biosynthesis C-methyltransferase UbiEAdd BLAST251

Proteomic databases

EPDiP0A887.
PaxDbiP0A887.
PRIDEiP0A887.

Interactioni

Protein-protein interaction databases

BioGridi4261082. 513 interactors.
DIPiDIP-31831N.
IntActiP0A887. 6 interactors.
MINTiMINT-1243319.
STRINGi511145.b3833.

Structurei

3D structure databases

ProteinModelPortaliP0A887.
SMRiP0A887.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni123 – 124S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DDZ. Bacteria.
COG2226. LUCA.
HOGENOMiHOG000249463.
InParanoidiP0A887.
KOiK03183.
OMAiGGLHRAW.
PhylomeDBiP0A887.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01813. MenG_UbiE_methyltr. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR004033. UbiE/COQ5_MeTrFase.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PfamiPF01209. Ubie_methyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITEiPS51608. SAM_MT_UBIE. 1 hit.
PS01183. UBIE_1. 1 hit.
PS01184. UBIE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL
60 70 80 90 100
WKRFTIDCSG VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM
110 120 130 140 150
LKMGREKLRN IGVIGNVEYV QANAEALPFP DNTFDCITIS FGLRNVTDKD
160 170 180 190 200
KALRSMYRVL KPGGRLLVLE FSKPIIEPLS KAYDAYSFHV LPRIGSLVAN
210 220 230 240 250
DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA GVVALHRGYK

F
Length:251
Mass (Da):28,073
Last modified:June 7, 2005 - v1
Checksum:iB1EEE209BDC68F5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67628.1.
U00096 Genomic DNA. Translation: AAT48227.1.
AP009048 Genomic DNA. Translation: BAE77468.1.
PIRiB65188.
RefSeqiWP_000227958.1. NZ_LN832404.1.
YP_026269.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48227; AAT48227; b3833.
BAE77468; BAE77468; BAE77468.
GeneIDi948926.
KEGGiecj:JW5581.
eco:b3833.
PATRICi32123167. VBIEscCol129921_3949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67628.1.
U00096 Genomic DNA. Translation: AAT48227.1.
AP009048 Genomic DNA. Translation: BAE77468.1.
PIRiB65188.
RefSeqiWP_000227958.1. NZ_LN832404.1.
YP_026269.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0A887.
SMRiP0A887.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261082. 513 interactors.
DIPiDIP-31831N.
IntActiP0A887. 6 interactors.
MINTiMINT-1243319.
STRINGi511145.b3833.

Chemistry databases

SwissLipidsiSLP:000001505.

Proteomic databases

EPDiP0A887.
PaxDbiP0A887.
PRIDEiP0A887.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48227; AAT48227; b3833.
BAE77468; BAE77468; BAE77468.
GeneIDi948926.
KEGGiecj:JW5581.
eco:b3833.
PATRICi32123167. VBIEscCol129921_3949.

Organism-specific databases

EchoBASEiEB1441.
EcoGeneiEG11473. ubiE.

Phylogenomic databases

eggNOGiENOG4105DDZ. Bacteria.
COG2226. LUCA.
HOGENOMiHOG000249463.
InParanoidiP0A887.
KOiK03183.
OMAiGGLHRAW.
PhylomeDBiP0A887.

Enzyme and pathway databases

UniPathwayiUPA00079; UER00169.
UPA00232.
BioCyciEcoCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
ECOL316407:JW5581-MONOMER.
MetaCyc:2-OCTAPRENYL-METHOXY-BENZOQ-METH-MONOMER.
BRENDAi2.1.1.163. 2026.

Miscellaneous databases

PROiP0A887.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01813. MenG_UbiE_methyltr. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR004033. UbiE/COQ5_MeTrFase.
IPR023576. UbiE/COQ5_MeTrFase_CS.
[Graphical view]
PfamiPF01209. Ubie_methyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITEiPS51608. SAM_MT_UBIE. 1 hit.
PS01183. UBIE_1. 1 hit.
PS01184. UBIE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBIE_ECOLI
AccessioniPrimary (citable) accession number: P0A887
Secondary accession number(s): P27851, Q2M8D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.