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Protein

Trp operon repressor

Gene

trpR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi68 – 91Add BLAST24

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00423.
ECOL316407:JW4356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trp operon repressor
Gene namesi
Name:trpR
Synonyms:rtrY
Ordered Locus Names:b4393, JW4356
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11029. trpR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001964942 – 108Trp operon repressorAdd BLAST107

Proteomic databases

PaxDbiP0A881.
PRIDEiP0A881.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4260801. 6 interactors.
DIPiDIP-48204N.
IntActiP0A881. 3 interactors.
STRINGi511145.b4393.

Chemistry databases

BindingDBiP0A881.

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 31Combined sources23
Helixi35 – 42Combined sources8
Helixi45 – 63Combined sources19
Helixi68 – 75Combined sources8
Helixi79 – 91Combined sources13
Helixi94 – 104Combined sources11
Turni105 – 107Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CO0NMR-A/B2-108[»]
1JHGX-ray1.30A8-108[»]
1MI7X-ray2.50R2-108[»]
1RCSNMR-A/B4-107[»]
1TROX-ray1.90A/C/E/G1-108[»]
1TRRX-ray2.40A/B/D/E/G/H/J/K2-108[»]
1WRPX-ray2.20R2-108[»]
1WRSNMR-R/S4-108[»]
1WRTNMR-R/S4-108[»]
1ZT9X-ray2.00A/B/D/E2-108[»]
2OZ9X-ray1.65R2-108[»]
3SSWX-ray1.67N/R2-108[»]
3SSXX-ray1.58N/R2-108[»]
3WRPX-ray1.80A1-108[»]
ProteinModelPortaliP0A881.
SMRiP0A881.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A881.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpR family.Curated

Phylogenomic databases

eggNOGiENOG4105R9M. Bacteria.
COG2973. LUCA.
HOGENOMiHOG000288846.
InParanoidiP0A881.
KOiK03720.
OMAiGQMSQRE.

Family and domain databases

Gene3Di1.10.1270.10. 1 hit.
HAMAPiMF_00475. Trp_repressor. 1 hit.
InterProiIPR000831. Trp_repress.
IPR013335. Trp_repress_bac.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF01371. Trp_repressor. 1 hit.
[Graphical view]
PIRSFiPIRSF003196. Trp_repressor. 1 hit.
ProDomiPD012582. Trp_repress_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF48295. SSF48295. 1 hit.
TIGRFAMsiTIGR01321. TrpR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA
60 70 80 90 100
LGTRVRIVEE LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL

EEVLLKSD
Length:108
Mass (Da):12,355
Last modified:January 23, 2007 - v2
Checksum:iFDFF8A60EC4FE7BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26L → V in AAA72134 (PubMed:7841459).Curated1
Sequence conflicti56R → A in AAA72134 (PubMed:7841459).Curated1
Sequence conflicti100L → M in AAA72134 (PubMed:7841459).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01715 Genomic DNA. Translation: AAA72140.1.
L13768 Genomic DNA. Translation: AAA72134.1.
U14003 Genomic DNA. Translation: AAA97289.1.
U00096 Genomic DNA. Translation: AAC77346.1.
AP009048 Genomic DNA. Translation: BAE78382.1.
M69185 Genomic DNA. No translation available.
PIRiA03568. RPECW.
RefSeqiNP_418810.1. NC_000913.3.
WP_000068679.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77346; AAC77346; b4393.
BAE78382; BAE78382; BAE78382.
GeneIDi948917.
KEGGiecj:JW4356.
eco:b4393.
PATRICi32124404. VBIEscCol129921_4542.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01715 Genomic DNA. Translation: AAA72140.1.
L13768 Genomic DNA. Translation: AAA72134.1.
U14003 Genomic DNA. Translation: AAA97289.1.
U00096 Genomic DNA. Translation: AAC77346.1.
AP009048 Genomic DNA. Translation: BAE78382.1.
M69185 Genomic DNA. No translation available.
PIRiA03568. RPECW.
RefSeqiNP_418810.1. NC_000913.3.
WP_000068679.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CO0NMR-A/B2-108[»]
1JHGX-ray1.30A8-108[»]
1MI7X-ray2.50R2-108[»]
1RCSNMR-A/B4-107[»]
1TROX-ray1.90A/C/E/G1-108[»]
1TRRX-ray2.40A/B/D/E/G/H/J/K2-108[»]
1WRPX-ray2.20R2-108[»]
1WRSNMR-R/S4-108[»]
1WRTNMR-R/S4-108[»]
1ZT9X-ray2.00A/B/D/E2-108[»]
2OZ9X-ray1.65R2-108[»]
3SSWX-ray1.67N/R2-108[»]
3SSXX-ray1.58N/R2-108[»]
3WRPX-ray1.80A1-108[»]
ProteinModelPortaliP0A881.
SMRiP0A881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260801. 6 interactors.
DIPiDIP-48204N.
IntActiP0A881. 3 interactors.
STRINGi511145.b4393.

Chemistry databases

BindingDBiP0A881.

Proteomic databases

PaxDbiP0A881.
PRIDEiP0A881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77346; AAC77346; b4393.
BAE78382; BAE78382; BAE78382.
GeneIDi948917.
KEGGiecj:JW4356.
eco:b4393.
PATRICi32124404. VBIEscCol129921_4542.

Organism-specific databases

EchoBASEiEB1022.
EcoGeneiEG11029. trpR.

Phylogenomic databases

eggNOGiENOG4105R9M. Bacteria.
COG2973. LUCA.
HOGENOMiHOG000288846.
InParanoidiP0A881.
KOiK03720.
OMAiGQMSQRE.

Enzyme and pathway databases

BioCyciEcoCyc:PD00423.
ECOL316407:JW4356-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A881.
PROiP0A881.

Family and domain databases

Gene3Di1.10.1270.10. 1 hit.
HAMAPiMF_00475. Trp_repressor. 1 hit.
InterProiIPR000831. Trp_repress.
IPR013335. Trp_repress_bac.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF01371. Trp_repressor. 1 hit.
[Graphical view]
PIRSFiPIRSF003196. Trp_repressor. 1 hit.
ProDomiPD012582. Trp_repress_bac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF48295. SSF48295. 1 hit.
TIGRFAMsiTIGR01321. TrpR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRPR_ECOLI
AccessioniPrimary (citable) accession number: P0A881
Secondary accession number(s): P03032, Q2M5S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

PubMed:7841459 sequence was originally thought to originate from S.typhimurium, but seems to come from an unknown E.coli strain.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.