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Protein

Trp operon repressor

Gene

trpR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein is an aporepressor. When complexed with L-tryptophan it binds the operator region of the trp operon (5'-ACTAGT-'3') and prevents the initiation of transcription. The complex also regulates trp repressor biosynthesis by binding to its regulatory region.

Caution

PubMed:7841459 sequence was originally thought to originate from S.typhimurium, but seems to come from an unknown E.coli strain.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi68 – 91Add BLAST24

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • DNA binding transcription factor activity Source: InterPro
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00423

Names & Taxonomyi

Protein namesi
Recommended name:
Trp operon repressor
Gene namesi
Name:trpR
Synonyms:rtrY
Ordered Locus Names:b4393, JW4356
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11029 trpR

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001964942 – 108Trp operon repressorAdd BLAST107

Proteomic databases

PaxDbiP0A881
PRIDEiP0A881

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4260801, 6 interactors
DIPiDIP-48204N
IntActiP0A881, 3 interactors
STRINGi316385.ECDH10B_4551

Chemistry databases

BindingDBiP0A881

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 31Combined sources23
Helixi35 – 42Combined sources8
Helixi45 – 63Combined sources19
Helixi68 – 75Combined sources8
Helixi79 – 91Combined sources13
Helixi94 – 104Combined sources11
Turni105 – 107Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CO0NMR-A/B2-108[»]
1JHGX-ray1.30A8-108[»]
1MI7X-ray2.50R2-108[»]
1RCSNMR-A/B4-107[»]
1TROX-ray1.90A/C/E/G1-108[»]
1TRRX-ray2.40A/B/D/E/G/H/J/K2-108[»]
1WRPX-ray2.20R2-108[»]
1WRSNMR-R/S4-108[»]
1WRTNMR-R/S4-108[»]
1ZT9X-ray2.00A/B/D/E2-108[»]
2OZ9X-ray1.65R2-108[»]
3SSWX-ray1.67N/R2-108[»]
3SSXX-ray1.58N/R2-108[»]
3WRPX-ray1.80A1-108[»]
ProteinModelPortaliP0A881
SMRiP0A881
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A881

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpR family.Curated

Phylogenomic databases

eggNOGiENOG4105R9M Bacteria
COG2973 LUCA
HOGENOMiHOG000288846
InParanoidiP0A881
KOiK03720
OMAiMMTPDER

Family and domain databases

Gene3Di1.10.1270.10, 1 hit
HAMAPiMF_00475 Trp_repressor, 1 hit
InterProiView protein in InterPro
IPR000831 Trp_repress
IPR013335 Trp_repress_bac
IPR010921 Trp_repressor/repl_initiator
IPR038116 TrpR-like_sf
PANTHERiPTHR38025 PTHR38025, 1 hit
PfamiView protein in Pfam
PF01371 Trp_repressor, 1 hit
PIRSFiPIRSF003196 Trp_repressor, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD012582 Trp_repress_bac, 1 hit
SUPFAMiSSF48295 SSF48295, 1 hit
TIGRFAMsiTIGR01321 TrpR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA
60 70 80 90 100
LGTRVRIVEE LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL

EEVLLKSD
Length:108
Mass (Da):12,355
Last modified:January 23, 2007 - v2
Checksum:iFDFF8A60EC4FE7BE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26L → V in AAA72134 (PubMed:7841459).Curated1
Sequence conflicti56R → A in AAA72134 (PubMed:7841459).Curated1
Sequence conflicti100L → M in AAA72134 (PubMed:7841459).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01715 Genomic DNA Translation: AAA72140.1
L13768 Genomic DNA Translation: AAA72134.1
U14003 Genomic DNA Translation: AAA97289.1
U00096 Genomic DNA Translation: AAC77346.1
AP009048 Genomic DNA Translation: BAE78382.1
M69185 Genomic DNA No translation available.
PIRiA03568 RPECW
RefSeqiNP_418810.1, NC_000913.3
WP_000068679.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77346; AAC77346; b4393
BAE78382; BAE78382; BAE78382
GeneIDi948917
KEGGiecj:JW4356
eco:b4393
PATRICifig|511145.12.peg.4542

Similar proteinsi

Entry informationi

Entry nameiTRPR_ECOLI
AccessioniPrimary (citable) accession number: P0A881
Secondary accession number(s): P03032, Q2M5S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health