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Protein

Tryptophan synthase beta chain

Gene

trpB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei62Nucleophile1 Publication1
Active sitei86Proton donor1 Publication1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • pyridoxal phosphate binding Source: EcoliWiki
  • tryptophan synthase activity Source: EcoCyc

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: EcoliWiki
  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • tryptophan biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:TRYPSYN-BPROTEIN.
ECOL316407:JW1253-MONOMER.
MetaCyc:TRYPSYN-BPROTEIN.
BRENDAi4.2.1.20. 2026.
SABIO-RKP0A879.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain (EC:4.2.1.20)
Gene namesi
Name:trpB
Ordered Locus Names:b1261, JW1253
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11025. trpB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000989482 – 397Tryptophan synthase beta chainAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

EPDiP0A879.
PaxDbiP0A879.
PRIDEiP0A879.

2D gel databases

SWISS-2DPAGEP0A879.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1123130,EBI-1123130

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259543. 9 interactors.
DIPiDIP-1036N.
IntActiP0A879. 8 interactors.
MINTiMINT-7711989.
STRINGi511145.b1261.

Structurei

Secondary structure

1397
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 21Combined sources3
Helixi22 – 37Combined sources16
Helixi39 – 51Combined sources13
Beta strandi59 – 61Combined sources3
Helixi63 – 66Combined sources4
Beta strandi69 – 77Combined sources9
Helixi78 – 80Combined sources3
Helixi87 – 100Combined sources14
Beta strandi105 – 109Combined sources5
Beta strandi111 – 113Combined sources3
Helixi114 – 125Combined sources12
Beta strandi129 – 135Combined sources7
Helixi136 – 141Combined sources6
Helixi143 – 151Combined sources9
Beta strandi155 – 159Combined sources5
Helixi166 – 180Combined sources15
Turni181 – 183Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Helixi197 – 205Combined sources9
Helixi207 – 220Combined sources14
Beta strandi225 – 230Combined sources6
Beta strandi232 – 234Combined sources3
Helixi235 – 241Combined sources7
Helixi242 – 244Combined sources3
Beta strandi245 – 247Combined sources3
Beta strandi250 – 259Combined sources10
Turni262 – 264Combined sources3
Helixi270 – 273Combined sources4
Beta strandi274 – 279Combined sources6
Beta strandi282 – 286Combined sources5
Helixi311 – 318Combined sources8
Beta strandi321 – 328Combined sources8
Helixi329 – 343Combined sources15
Helixi349 – 363Combined sources15
Beta strandi370 – 376Combined sources7
Beta strandi378 – 380Combined sources3
Helixi381 – 383Combined sources3
Helixi384 – 393Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DH5X-ray2.90A1-397[»]
2DH6X-ray3.00A1-397[»]
ProteinModelPortaliP0A879.
SMRiP0A879.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A879.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiENOG4105CG0. Bacteria.
COG0133. LUCA.
HOGENOMiHOG000161710.
InParanoidiP0A879.
KOiK01696.
OMAiCQKEGII.
PhylomeDBiP0A879.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A879-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLLNPYFG EFGGMYVPQI LMPALRQLEE AFVSAQKDPE FQAQFNDLLK
60 70 80 90 100
NYAGRPTALT KCQNITAGTN TTLYLKREDL LHGGAHKTNQ VLGQALLAKR
110 120 130 140 150
MGKTEIIAET GAGQHGVASA LASALLGLKC RIYMGAKDVE RQSPNVFRMR
160 170 180 190 200
LMGAEVIPVH SGSATLKDAC NEALRDWSGS YETAHYMLGT AAGPHPYPTI
210 220 230 240 250
VREFQRMIGE ETKAQILERE GRLPDAVIAC VGGGSNAIGM FADFINETNV
260 270 280 290 300
GLIGVEPGGH GIETGEHGAP LKHGRVGIYF GMKAPMMQTE DGQIEESYSI
310 320 330 340 350
SAGLDFPSVG PQHAYLNSTG RADYVSITDD EALEAFKTLC LHEGIIPALE
360 370 380 390
SSHALAHALK MMRENPDKEQ LLVVNLSGRG DKDIFTVHDI LKARGEI
Length:397
Mass (Da):42,983
Last modified:January 23, 2007 - v2
Checksum:i16CF49FBD738F06F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78E → K AA sequence (PubMed:6985892).Curated1
Sequence conflicti365N → Z AA sequence (PubMed:4552018).Curated1
Sequence conflicti368 – 369KE → BK AA sequence (PubMed:4552018).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti281G → R in mutant TRPB8. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00365 Genomic DNA. Translation: CAA23663.1.
V00372 Genomic DNA. Translation: CAA23674.1.
U00096 Genomic DNA. Translation: AAC74343.1.
AP009048 Genomic DNA. Translation: BAA14793.1.
J01714 Genomic DNA. Translation: AAA57300.1.
U23489 Genomic DNA. Translation: AAB60034.1.
U23490 Genomic DNA. Translation: AAA65139.1.
U23492 Genomic DNA. Translation: AAA65151.1.
U23493 Genomic DNA. Translation: AAA65157.1.
U23500 Genomic DNA. Translation: AAA65175.1.
U25417 Genomic DNA. Translation: AAA73790.1.
U25418 Genomic DNA. Translation: AAA73796.1.
U25419 Genomic DNA. Translation: AAA73802.1.
U25422 Genomic DNA. Translation: AAA73820.1.
U25423 Genomic DNA. Translation: AAA73826.1.
U25426 Genomic DNA. Translation: AAA73838.1.
U25427 Genomic DNA. Translation: AAA73844.1.
U25428 Genomic DNA. Translation: AAA73850.1.
U25429 Genomic DNA. Translation: AAA73856.1.
PIRiH64873. TSECB.
RefSeqiNP_415777.1. NC_000913.3.
WP_000209520.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74343; AAC74343; b1261.
BAA14793; BAA14793; BAA14793.
GeneIDi945768.
KEGGiecj:JW1253.
eco:b1261.
PATRICi32117782. VBIEscCol129921_1311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00365 Genomic DNA. Translation: CAA23663.1.
V00372 Genomic DNA. Translation: CAA23674.1.
U00096 Genomic DNA. Translation: AAC74343.1.
AP009048 Genomic DNA. Translation: BAA14793.1.
J01714 Genomic DNA. Translation: AAA57300.1.
U23489 Genomic DNA. Translation: AAB60034.1.
U23490 Genomic DNA. Translation: AAA65139.1.
U23492 Genomic DNA. Translation: AAA65151.1.
U23493 Genomic DNA. Translation: AAA65157.1.
U23500 Genomic DNA. Translation: AAA65175.1.
U25417 Genomic DNA. Translation: AAA73790.1.
U25418 Genomic DNA. Translation: AAA73796.1.
U25419 Genomic DNA. Translation: AAA73802.1.
U25422 Genomic DNA. Translation: AAA73820.1.
U25423 Genomic DNA. Translation: AAA73826.1.
U25426 Genomic DNA. Translation: AAA73838.1.
U25427 Genomic DNA. Translation: AAA73844.1.
U25428 Genomic DNA. Translation: AAA73850.1.
U25429 Genomic DNA. Translation: AAA73856.1.
PIRiH64873. TSECB.
RefSeqiNP_415777.1. NC_000913.3.
WP_000209520.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DH5X-ray2.90A1-397[»]
2DH6X-ray3.00A1-397[»]
ProteinModelPortaliP0A879.
SMRiP0A879.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259543. 9 interactors.
DIPiDIP-1036N.
IntActiP0A879. 8 interactors.
MINTiMINT-7711989.
STRINGi511145.b1261.

2D gel databases

SWISS-2DPAGEP0A879.

Proteomic databases

EPDiP0A879.
PaxDbiP0A879.
PRIDEiP0A879.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74343; AAC74343; b1261.
BAA14793; BAA14793; BAA14793.
GeneIDi945768.
KEGGiecj:JW1253.
eco:b1261.
PATRICi32117782. VBIEscCol129921_1311.

Organism-specific databases

EchoBASEiEB1018.
EcoGeneiEG11025. trpB.

Phylogenomic databases

eggNOGiENOG4105CG0. Bacteria.
COG0133. LUCA.
HOGENOMiHOG000161710.
InParanoidiP0A879.
KOiK01696.
OMAiCQKEGII.
PhylomeDBiP0A879.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciEcoCyc:TRYPSYN-BPROTEIN.
ECOL316407:JW1253-MONOMER.
MetaCyc:TRYPSYN-BPROTEIN.
BRENDAi4.2.1.20. 2026.
SABIO-RKP0A879.

Miscellaneous databases

EvolutionaryTraceiP0A879.
PROiP0A879.

Family and domain databases

CDDicd06446. Trp-synth_B. 1 hit.
HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPB_ECOLI
AccessioniPrimary (citable) accession number: P0A879
Secondary accession number(s): P00932, P78146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.