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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei49Proton acceptorUniRule annotation1
Active sitei60Proton acceptorUniRule annotation1

GO - Molecular functioni

  • lyase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: GO_Central
  • tryptophan synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: EcoliWiki
  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • tryptophan biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:TRYPSYN-APROTEIN.
ECOL316407:JW1252-MONOMER.
MetaCyc:TRYPSYN-APROTEIN.
BRENDAi4.2.1.20. 2026.
SABIO-RKP0A877.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:b1260, JW1252
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11024. trpA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000987781 – 268Tryptophan synthase alpha chainAdd BLAST268

Proteomic databases

EPDiP0A877.
PaxDbiP0A877.
PRIDEiP0A877.

2D gel databases

SWISS-2DPAGEP0A877.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Protein-protein interaction databases

BioGridi4260129. 9 interactors.
DIPiDIP-35957N.
IntActiP0A877. 6 interactors.
STRINGi511145.b1260.

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 12Combined sources10
Turni13 – 15Combined sources3
Beta strandi18 – 24Combined sources7
Beta strandi26 – 28Combined sources3
Helixi30 – 43Combined sources14
Beta strandi46 – 51Combined sources6
Helixi70 – 73Combined sources4
Helixi78 – 91Combined sources14
Beta strandi93 – 95Combined sources3
Beta strandi97 – 101Combined sources5
Helixi103 – 109Combined sources7
Helixi111 – 121Combined sources11
Beta strandi125 – 128Combined sources4
Helixi133 – 135Combined sources3
Helixi137 – 145Combined sources9
Beta strandi149 – 151Combined sources3
Helixi160 – 169Combined sources10
Beta strandi174 – 176Combined sources3
Helixi179 – 181Combined sources3
Helixi193 – 202Combined sources10
Beta strandi208 – 211Combined sources4
Helixi217 – 225Combined sources9
Beta strandi229 – 233Combined sources5
Helixi236 – 242Combined sources7
Turni244 – 246Combined sources3
Helixi248 – 264Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V7YX-ray2.50A/B1-268[»]
1WQ5X-ray2.30A/B1-268[»]
1XC4X-ray2.80A/B1-268[»]
1XCFX-ray1.80A/B1-268[»]
DisProtiDP00252.
ProteinModelPortaliP0A877.
SMRiP0A877.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A877.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223814.
InParanoidiP0A877.
KOiK01695.
OMAiDYNPHIP.
PhylomeDBiP0A877.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL
60 70 80 90 100
GIPFSDPLAD GPTIQNATLR AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL
110 120 130 140 150
MYANLVFNKG IDEFYAQCEK VGVDSVLVAD VPVEESAPFR QAALRHNVAP
160 170 180 190 200
IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLNHLVAKL
210 220 230 240 250
KEYNAAPPLQ GFGISAPDQV KAAIDAGAAG AISGSAIVKI IEQHINEPEK
260
MLAALKVFVQ PMKAATRS
Length:268
Mass (Da):28,724
Last modified:July 21, 1986 - v1
Checksum:i028223DB2B666CC6
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti209 – 224LQGFG…VKAAI → IAGFWYFRPGSGKSSD in mutant TrpA46-Asp-PR3. Add BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00364 Genomic DNA. Translation: CAA23662.1.
V00372 Genomic DNA. Translation: CAA23675.1.
J01714 Genomic DNA. Translation: AAA57301.1.
U23489 Genomic DNA. Translation: AAB60035.1.
U23490 Genomic DNA. Translation: AAA65140.1.
U23491 Genomic DNA. Translation: AAA65146.1.
U23492 Genomic DNA. Translation: AAA65152.1.
U23494 Genomic DNA. Translation: AAA65164.1.
U25417 Genomic DNA. Translation: AAA73791.1.
U25418 Genomic DNA. Translation: AAA73797.1.
U25419 Genomic DNA. Translation: AAA73803.1.
U25420 Genomic DNA. Translation: AAA73809.1.
U25421 Genomic DNA. Translation: AAA73815.1.
U25422 Genomic DNA. Translation: AAA73821.1.
U00096 Genomic DNA. Translation: AAC74342.1.
AP009048 Genomic DNA. Translation: BAA14792.1.
X16698 Genomic DNA. Translation: CAA34671.1.
PIRiE93746. TSECA.
RefSeqiNP_415776.1. NC_000913.3.
WP_000443067.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74342; AAC74342; b1260.
BAA14792; BAA14792; BAA14792.
GeneIDi946204.
KEGGiecj:JW1252.
eco:b1260.
PATRICi32117780. VBIEscCol129921_1310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00364 Genomic DNA. Translation: CAA23662.1.
V00372 Genomic DNA. Translation: CAA23675.1.
J01714 Genomic DNA. Translation: AAA57301.1.
U23489 Genomic DNA. Translation: AAB60035.1.
U23490 Genomic DNA. Translation: AAA65140.1.
U23491 Genomic DNA. Translation: AAA65146.1.
U23492 Genomic DNA. Translation: AAA65152.1.
U23494 Genomic DNA. Translation: AAA65164.1.
U25417 Genomic DNA. Translation: AAA73791.1.
U25418 Genomic DNA. Translation: AAA73797.1.
U25419 Genomic DNA. Translation: AAA73803.1.
U25420 Genomic DNA. Translation: AAA73809.1.
U25421 Genomic DNA. Translation: AAA73815.1.
U25422 Genomic DNA. Translation: AAA73821.1.
U00096 Genomic DNA. Translation: AAC74342.1.
AP009048 Genomic DNA. Translation: BAA14792.1.
X16698 Genomic DNA. Translation: CAA34671.1.
PIRiE93746. TSECA.
RefSeqiNP_415776.1. NC_000913.3.
WP_000443067.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V7YX-ray2.50A/B1-268[»]
1WQ5X-ray2.30A/B1-268[»]
1XC4X-ray2.80A/B1-268[»]
1XCFX-ray1.80A/B1-268[»]
DisProtiDP00252.
ProteinModelPortaliP0A877.
SMRiP0A877.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260129. 9 interactors.
DIPiDIP-35957N.
IntActiP0A877. 6 interactors.
STRINGi511145.b1260.

2D gel databases

SWISS-2DPAGEP0A877.

Proteomic databases

EPDiP0A877.
PaxDbiP0A877.
PRIDEiP0A877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74342; AAC74342; b1260.
BAA14792; BAA14792; BAA14792.
GeneIDi946204.
KEGGiecj:JW1252.
eco:b1260.
PATRICi32117780. VBIEscCol129921_1310.

Organism-specific databases

EchoBASEiEB1017.
EcoGeneiEG11024. trpA.

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223814.
InParanoidiP0A877.
KOiK01695.
OMAiDYNPHIP.
PhylomeDBiP0A877.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciEcoCyc:TRYPSYN-APROTEIN.
ECOL316407:JW1252-MONOMER.
MetaCyc:TRYPSYN-APROTEIN.
BRENDAi4.2.1.20. 2026.
SABIO-RKP0A877.

Miscellaneous databases

EvolutionaryTraceiP0A877.
PROiP0A877.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_ECOLI
AccessioniPrimary (citable) accession number: P0A877
Secondary accession number(s): P00928, Q47669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.