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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Bifunctional protein TrpGD (trpGD), Anthranilate synthase component 1 (trpE)
  2. Bifunctional protein TrpGD (trpGD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton acceptorUniRule annotation
Active sitei60 – 601Proton acceptorUniRule annotation

GO - Molecular functioni

  • lyase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: GO_Central
  • tryptophan synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: EcoliWiki
  • tryptophan biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:TRYPSYN-APROTEIN.
ECOL316407:JW1252-MONOMER.
MetaCyc:TRYPSYN-APROTEIN.
BRENDAi4.2.1.20. 2026.
SABIO-RKP0A877.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:b1260, JW1252
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11024. trpA.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Tryptophan synthase alpha chainPRO_0000098778Add
BLAST

Proteomic databases

EPDiP0A877.
PaxDbiP0A877.
PRIDEiP0A877.

2D gel databases

SWISS-2DPAGEP0A877.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Protein-protein interaction databases

BioGridi4260129. 9 interactions.
DIPiDIP-35957N.
IntActiP0A877. 6 interactions.
STRINGi511145.b1260.

Structurei

Secondary structure

1
268
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1210Combined sources
Turni13 – 153Combined sources
Beta strandi18 – 247Combined sources
Beta strandi26 – 283Combined sources
Helixi30 – 4314Combined sources
Beta strandi46 – 516Combined sources
Helixi70 – 734Combined sources
Helixi78 – 9114Combined sources
Beta strandi93 – 953Combined sources
Beta strandi97 – 1015Combined sources
Helixi103 – 1097Combined sources
Helixi111 – 12111Combined sources
Beta strandi125 – 1284Combined sources
Helixi133 – 1353Combined sources
Helixi137 – 1459Combined sources
Beta strandi149 – 1513Combined sources
Helixi160 – 16910Combined sources
Beta strandi174 – 1763Combined sources
Helixi179 – 1813Combined sources
Helixi193 – 20210Combined sources
Beta strandi208 – 2114Combined sources
Helixi217 – 2259Combined sources
Beta strandi229 – 2335Combined sources
Helixi236 – 2427Combined sources
Turni244 – 2463Combined sources
Helixi248 – 26417Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V7YX-ray2.50A/B1-268[»]
1WQ5X-ray2.30A/B1-268[»]
1XC4X-ray2.80A/B1-268[»]
1XCFX-ray1.80A/B1-268[»]
DisProtiDP00252.
ProteinModelPortaliP0A877.
SMRiP0A877. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A877.

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223814.
InParanoidiP0A877.
KOiK01695.
OMAiDYNPHIP.
PhylomeDBiP0A877.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A877-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL
60 70 80 90 100
GIPFSDPLAD GPTIQNATLR AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL
110 120 130 140 150
MYANLVFNKG IDEFYAQCEK VGVDSVLVAD VPVEESAPFR QAALRHNVAP
160 170 180 190 200
IFICPPNADD DLLRQIASYG RGYTYLLSRA GVTGAENRAA LPLNHLVAKL
210 220 230 240 250
KEYNAAPPLQ GFGISAPDQV KAAIDAGAAG AISGSAIVKI IEQHINEPEK
260
MLAALKVFVQ PMKAATRS
Length:268
Mass (Da):28,724
Last modified:July 21, 1986 - v1
Checksum:i028223DB2B666CC6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti209 – 22416LQGFG…VKAAI → IAGFWYFRPGSGKSSD in mutant TrpA46-Asp-PR3.
Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00364 Genomic DNA. Translation: CAA23662.1.
V00372 Genomic DNA. Translation: CAA23675.1.
J01714 Genomic DNA. Translation: AAA57301.1.
U23489 Genomic DNA. Translation: AAB60035.1.
U23490 Genomic DNA. Translation: AAA65140.1.
U23491 Genomic DNA. Translation: AAA65146.1.
U23492 Genomic DNA. Translation: AAA65152.1.
U23494 Genomic DNA. Translation: AAA65164.1.
U25417 Genomic DNA. Translation: AAA73791.1.
U25418 Genomic DNA. Translation: AAA73797.1.
U25419 Genomic DNA. Translation: AAA73803.1.
U25420 Genomic DNA. Translation: AAA73809.1.
U25421 Genomic DNA. Translation: AAA73815.1.
U25422 Genomic DNA. Translation: AAA73821.1.
U00096 Genomic DNA. Translation: AAC74342.1.
AP009048 Genomic DNA. Translation: BAA14792.1.
X16698 Genomic DNA. Translation: CAA34671.1.
PIRiE93746. TSECA.
RefSeqiNP_415776.1. NC_000913.3.
WP_000443067.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74342; AAC74342; b1260.
BAA14792; BAA14792; BAA14792.
GeneIDi946204.
KEGGiecj:JW1252.
eco:b1260.
PATRICi32117780. VBIEscCol129921_1310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00364 Genomic DNA. Translation: CAA23662.1.
V00372 Genomic DNA. Translation: CAA23675.1.
J01714 Genomic DNA. Translation: AAA57301.1.
U23489 Genomic DNA. Translation: AAB60035.1.
U23490 Genomic DNA. Translation: AAA65140.1.
U23491 Genomic DNA. Translation: AAA65146.1.
U23492 Genomic DNA. Translation: AAA65152.1.
U23494 Genomic DNA. Translation: AAA65164.1.
U25417 Genomic DNA. Translation: AAA73791.1.
U25418 Genomic DNA. Translation: AAA73797.1.
U25419 Genomic DNA. Translation: AAA73803.1.
U25420 Genomic DNA. Translation: AAA73809.1.
U25421 Genomic DNA. Translation: AAA73815.1.
U25422 Genomic DNA. Translation: AAA73821.1.
U00096 Genomic DNA. Translation: AAC74342.1.
AP009048 Genomic DNA. Translation: BAA14792.1.
X16698 Genomic DNA. Translation: CAA34671.1.
PIRiE93746. TSECA.
RefSeqiNP_415776.1. NC_000913.3.
WP_000443067.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V7YX-ray2.50A/B1-268[»]
1WQ5X-ray2.30A/B1-268[»]
1XC4X-ray2.80A/B1-268[»]
1XCFX-ray1.80A/B1-268[»]
DisProtiDP00252.
ProteinModelPortaliP0A877.
SMRiP0A877. Positions 1-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260129. 9 interactions.
DIPiDIP-35957N.
IntActiP0A877. 6 interactions.
STRINGi511145.b1260.

2D gel databases

SWISS-2DPAGEP0A877.

Proteomic databases

EPDiP0A877.
PaxDbiP0A877.
PRIDEiP0A877.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74342; AAC74342; b1260.
BAA14792; BAA14792; BAA14792.
GeneIDi946204.
KEGGiecj:JW1252.
eco:b1260.
PATRICi32117780. VBIEscCol129921_1310.

Organism-specific databases

EchoBASEiEB1017.
EcoGeneiEG11024. trpA.

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223814.
InParanoidiP0A877.
KOiK01695.
OMAiDYNPHIP.
PhylomeDBiP0A877.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciEcoCyc:TRYPSYN-APROTEIN.
ECOL316407:JW1252-MONOMER.
MetaCyc:TRYPSYN-APROTEIN.
BRENDAi4.2.1.20. 2026.
SABIO-RKP0A877.

Miscellaneous databases

EvolutionaryTraceiP0A877.
PROiP0A877.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_ECOLI
AccessioniPrimary (citable) accession number: P0A877
Secondary accession number(s): P00928, Q47669
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.