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Protein

Thiol peroxidase

Gene

tpx

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has antioxidant activity. Could remove peroxides or H2O2 within the catalase- and peroxidase-deficient periplasmic space.

GO - Molecular functioni

  • hydroperoxide reductase activity Source: EcoCyc
  • thioredoxin peroxidase activity Source: EcoCyc

GO - Biological processi

  • cell redox homeostasis Source: UniProtKB-HAMAP
  • cellular response to oxidative stress Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Antioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BioCyciEcoCyc:G6660-MONOMER.
ECOL316407:JW1317-MONOMER.
MetaCyc:G6660-MONOMER.
BRENDAi1.11.1.7. 2026.

Protein family/group databases

PeroxiBasei6007. EcoTPx.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol peroxidase (EC:1.11.1.-)
Alternative name(s):
Scavengase P20
Gene namesi
Name:tpx
Synonyms:yzzJ
Ordered Locus Names:b1324, JW1317
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12672. tpx.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
  • cytosol Source: EcoCyc
  • periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001878772 – 168Thiol peroxidaseAdd BLAST167

Proteomic databases

EPDiP0A862.
PaxDbiP0A862.
PRIDEiP0A862.

2D gel databases

SWISS-2DPAGEP0A862.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ycfPP0A8E13EBI-369411,EBI-9129402

Protein-protein interaction databases

BioGridi4260151. 128 interactors.
850247. 1 interactor.
DIPiDIP-31857N.
IntActiP0A862. 9 interactors.
STRINGi511145.b1324.

Structurei

Secondary structure

1168
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Beta strandi10 – 14Combined sources5
Beta strandi29 – 31Combined sources3
Beta strandi37 – 39Combined sources3
Helixi40 – 43Combined sources4
Beta strandi46 – 52Combined sources7
Beta strandi56 – 59Combined sources4
Helixi61 – 71Combined sources11
Helixi72 – 74Combined sources3
Beta strandi75 – 86Combined sources12
Helixi88 – 98Combined sources11
Beta strandi102 – 107Combined sources6
Helixi112 – 117Combined sources6
Turni126 – 129Combined sources4
Beta strandi133 – 138Combined sources6
Beta strandi142 – 149Combined sources8
Helixi159 – 164Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QXHX-ray2.20A/B2-168[»]
3HVSX-ray1.80A/B2-168[»]
3HVVX-ray1.75A2-168[»]
3HVXX-ray2.12A/B/C/D2-168[»]
3I43X-ray2.80A/B2-168[»]
4AF2X-ray1.97A1-168[»]
ProteinModelPortaliP0A862.
SMRiP0A862.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A862.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 168ThioredoxinAdd BLAST150

Sequence similaritiesi

Belongs to the AhpC/TSA family. Tpx subfamily.Curated
Contains 1 thioredoxin domain.Curated

Phylogenomic databases

eggNOGiENOG4108V1J. Bacteria.
COG2077. LUCA.
HOGENOMiHOG000022345.
InParanoidiP0A862.
KOiK11065.
OMAiKFNAQAN.
PhylomeDBiP0A862.

Family and domain databases

CDDicd03014. PRX_Atyp2cys. 1 hit.
Gene3Di3.40.30.10. 1 hit.
HAMAPiMF_00269. Tpx. 1 hit.
InterProiIPR002065. Put_TPX.
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
IPR018219. Tpx_CS.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
PS01265. TPX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQTVHFQGN PVTVANSIPQ AGSKAQTFTL VAKDLSDVTL GQFAGKRKVL
60 70 80 90 100
NIFPSIDTGV CAASVRKFNQ LATEIDNTVV LCISADLPFA QSRFCGAEGL
110 120 130 140 150
NNVITLSTFR NAEFLQAYGV AIADGPLKGL AARAVVVIDE NDNVIFSQLV
160
DEITTEPDYE AALAVLKA
Length:168
Mass (Da):17,835
Last modified:January 23, 2007 - v2
Checksum:iF95F39094A07DBB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61C → Y in AAC43517 (PubMed:7499381).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33213 Genomic DNA. Translation: AAC43517.1.
U93212 Genomic DNA. Translation: AAC45284.1.
U00096 Genomic DNA. Translation: AAC74406.1.
AP009048 Genomic DNA. Translation: BAA14906.1.
PIRiJC5504.
RefSeqiNP_415840.1. NC_000913.3.
WP_000084387.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74406; AAC74406; b1324.
BAA14906; BAA14906; BAA14906.
GeneIDi945880.
KEGGiecj:JW1317.
eco:b1324.
PATRICi32117922. VBIEscCol129921_1381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33213 Genomic DNA. Translation: AAC43517.1.
U93212 Genomic DNA. Translation: AAC45284.1.
U00096 Genomic DNA. Translation: AAC74406.1.
AP009048 Genomic DNA. Translation: BAA14906.1.
PIRiJC5504.
RefSeqiNP_415840.1. NC_000913.3.
WP_000084387.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QXHX-ray2.20A/B2-168[»]
3HVSX-ray1.80A/B2-168[»]
3HVVX-ray1.75A2-168[»]
3HVXX-ray2.12A/B/C/D2-168[»]
3I43X-ray2.80A/B2-168[»]
4AF2X-ray1.97A1-168[»]
ProteinModelPortaliP0A862.
SMRiP0A862.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260151. 128 interactors.
850247. 1 interactor.
DIPiDIP-31857N.
IntActiP0A862. 9 interactors.
STRINGi511145.b1324.

Protein family/group databases

PeroxiBasei6007. EcoTPx.

2D gel databases

SWISS-2DPAGEP0A862.

Proteomic databases

EPDiP0A862.
PaxDbiP0A862.
PRIDEiP0A862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74406; AAC74406; b1324.
BAA14906; BAA14906; BAA14906.
GeneIDi945880.
KEGGiecj:JW1317.
eco:b1324.
PATRICi32117922. VBIEscCol129921_1381.

Organism-specific databases

EchoBASEiEB2538.
EcoGeneiEG12672. tpx.

Phylogenomic databases

eggNOGiENOG4108V1J. Bacteria.
COG2077. LUCA.
HOGENOMiHOG000022345.
InParanoidiP0A862.
KOiK11065.
OMAiKFNAQAN.
PhylomeDBiP0A862.

Enzyme and pathway databases

BioCyciEcoCyc:G6660-MONOMER.
ECOL316407:JW1317-MONOMER.
MetaCyc:G6660-MONOMER.
BRENDAi1.11.1.7. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A862.
PROiP0A862.

Family and domain databases

CDDicd03014. PRX_Atyp2cys. 1 hit.
Gene3Di3.40.30.10. 1 hit.
HAMAPiMF_00269. Tpx. 1 hit.
InterProiIPR002065. Put_TPX.
IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
IPR018219. Tpx_CS.
[Graphical view]
PfamiPF08534. Redoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
PS01265. TPX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPX_ECOLI
AccessioniPrimary (citable) accession number: P0A862
Secondary accession number(s): P37901
, P57669, P76047, P77786
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.