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Protein

Protein TolB

Gene

tolB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • protein transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Bacteriocin transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER.
ECOL316407:JW5100-MONOMER.
MetaCyc:EG11008-MONOMER.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TolB
Gene namesi
Name:tolB
Ordered Locus Names:b0740, JW5100
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11008. tolB.

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 21212 PublicationsAdd
BLAST
Chaini22 – 430409Protein TolBPRO_0000034647Add
BLAST

Proteomic databases

EPDiP0A855.
PaxDbiP0A855.
PRIDEiP0A855.

2D gel databases

SWISS-2DPAGEP0A855.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
colP098835EBI-7180728,EBI-1029888From a different organism.
palP0A9124EBI-7180728,EBI-1124760
tolAP199345EBI-7180728,EBI-1120026

Protein-protein interaction databases

BioGridi4261828. 329 interactions.
DIPiDIP-48262N.
IntActiP0A855. 11 interactions.
MINTiMINT-7888580.
STRINGi511145.b0740.

Structurei

Secondary structure

1
430
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi26 – 294Combined sources
Beta strandi31 – 333Combined sources
Beta strandi34 – 396Combined sources
Beta strandi43 – 497Combined sources
Helixi54 – 6411Combined sources
Beta strandi67 – 704Combined sources
Helixi73 – 753Combined sources
Helixi83 – 853Combined sources
Helixi88 – 925Combined sources
Turni93 – 953Combined sources
Beta strandi98 – 1069Combined sources
Beta strandi112 – 1209Combined sources
Beta strandi122 – 1243Combined sources
Beta strandi128 – 1369Combined sources
Helixi138 – 1403Combined sources
Helixi141 – 15717Combined sources
Beta strandi166 – 1727Combined sources
Beta strandi174 – 1774Combined sources
Beta strandi180 – 1867Combined sources
Beta strandi193 – 2008Combined sources
Beta strandi202 – 2076Combined sources
Beta strandi211 – 2188Combined sources
Beta strandi225 – 2306Combined sources
Turni231 – 2333Combined sources
Beta strandi236 – 2405Combined sources
Beta strandi246 – 2516Combined sources
Beta strandi255 – 2628Combined sources
Beta strandi269 – 2746Combined sources
Turni275 – 2773Combined sources
Beta strandi280 – 2823Combined sources
Beta strandi286 – 2883Combined sources
Beta strandi290 – 2956Combined sources
Beta strandi299 – 3068Combined sources
Beta strandi313 – 3186Combined sources
Beta strandi330 – 33910Combined sources
Beta strandi343 – 3519Combined sources
Beta strandi356 – 3627Combined sources
Turni363 – 3653Combined sources
Beta strandi368 – 3703Combined sources
Beta strandi374 – 3763Combined sources
Beta strandi378 – 3825Combined sources
Beta strandi386 – 39510Combined sources
Beta strandi398 – 4058Combined sources
Beta strandi411 – 4133Combined sources
Beta strandi417 – 42610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C5KX-ray2.00A1-430[»]
1CRZX-ray1.95A28-430[»]
2HQSX-ray1.50A/B/D/F23-430[»]
2IVZX-ray2.00A/B/C/D1-430[»]
2W8BX-ray1.86A/B/D/F22-430[»]
3IAXX-ray2.60A1-430[»]
ProteinModelPortaliP0A855.
SMRiP0A855. Positions 23-430.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A855.

Family & Domainsi

Sequence similaritiesi

Belongs to the TolB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107R6Y. Bacteria.
COG0823. LUCA.
HOGENOMiHOG000004098.
InParanoidiP0A855.
KOiK03641.
OMAiVREPSWG.
PhylomeDBiP0A855.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.40.50.10070. 1 hit.
HAMAPiMF_00671. TolB. 1 hit.
InterProiIPR011659. PD40.
IPR014167. Tol-Pal_propeller_TolB.
IPR007195. TolB_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF07676. PD40. 4 hits.
PF04052. TolB_N. 1 hit.
[Graphical view]
SUPFAMiSSF52964. SSF52964. 1 hit.
TIGRFAMsiTIGR02800. propeller_TolB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA
60 70 80 90 100
PEDIGGIVAA DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV
110 120 130 140 150
VGQVTPNPDG SYNVAYQLVD TGGAPGTVLA QNSYKVNKQW LRYAGHTASD
160 170 180 190 200
EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY ELRVSDYDGY NQFVVHRSPQ
210 220 230 240 250
PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA SFPRHNGAPA
260 270 280 290 300
FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ
310 320 330 340 350
NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS
360 370 380 390 400
SNGGQQHIAK QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV
410 420 430
LNLVSTDGRF KARLPATDGQ VKFPAWSPYL
Length:430
Mass (Da):45,956
Last modified:June 7, 2005 - v1
Checksum:i508795C6E6886B52
GO

Sequence cautioni

The sequence AAA24684 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24684.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73834.1.
AP009048 Genomic DNA. Translation: BAA35406.2.
X65796 Genomic DNA. Translation: CAA46672.1.
PIRiC64810.
RefSeqiNP_415268.1. NC_000913.3.
WP_001295307.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740.
BAA35406; BAA35406; BAA35406.
GeneIDi945429.
KEGGiecj:JW5100.
eco:b0740.
PATRICi32116679. VBIEscCol129921_0772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24684.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73834.1.
AP009048 Genomic DNA. Translation: BAA35406.2.
X65796 Genomic DNA. Translation: CAA46672.1.
PIRiC64810.
RefSeqiNP_415268.1. NC_000913.3.
WP_001295307.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C5KX-ray2.00A1-430[»]
1CRZX-ray1.95A28-430[»]
2HQSX-ray1.50A/B/D/F23-430[»]
2IVZX-ray2.00A/B/C/D1-430[»]
2W8BX-ray1.86A/B/D/F22-430[»]
3IAXX-ray2.60A1-430[»]
ProteinModelPortaliP0A855.
SMRiP0A855. Positions 23-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261828. 329 interactions.
DIPiDIP-48262N.
IntActiP0A855. 11 interactions.
MINTiMINT-7888580.
STRINGi511145.b0740.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

2D gel databases

SWISS-2DPAGEP0A855.

Proteomic databases

EPDiP0A855.
PaxDbiP0A855.
PRIDEiP0A855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740.
BAA35406; BAA35406; BAA35406.
GeneIDi945429.
KEGGiecj:JW5100.
eco:b0740.
PATRICi32116679. VBIEscCol129921_0772.

Organism-specific databases

EchoBASEiEB1001.
EcoGeneiEG11008. tolB.

Phylogenomic databases

eggNOGiENOG4107R6Y. Bacteria.
COG0823. LUCA.
HOGENOMiHOG000004098.
InParanoidiP0A855.
KOiK03641.
OMAiVREPSWG.
PhylomeDBiP0A855.

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER.
ECOL316407:JW5100-MONOMER.
MetaCyc:EG11008-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A855.
PROiP0A855.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.40.50.10070. 1 hit.
HAMAPiMF_00671. TolB. 1 hit.
InterProiIPR011659. PD40.
IPR014167. Tol-Pal_propeller_TolB.
IPR007195. TolB_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF07676. PD40. 4 hits.
PF04052. TolB_N. 1 hit.
[Graphical view]
SUPFAMiSSF52964. SSF52964. 1 hit.
TIGRFAMsiTIGR02800. propeller_TolB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOLB_ECOLI
AccessioniPrimary (citable) accession number: P0A855
Secondary accession number(s): P19935
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.