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Protein

Protein TolB

Gene

tolB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria.

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • protein transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Bacteriocin transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER.
ECOL316407:JW5100-MONOMER.
MetaCyc:EG11008-MONOMER.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TolB
Gene namesi
Name:tolB
Ordered Locus Names:b0740, JW5100
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11008. tolB.

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 212 PublicationsAdd BLAST21
ChainiPRO_000003464722 – 430Protein TolBAdd BLAST409

Proteomic databases

EPDiP0A855.
PaxDbiP0A855.
PRIDEiP0A855.

2D gel databases

SWISS-2DPAGEP0A855.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
colP098835EBI-7180728,EBI-1029888From a different organism.
palP0A9124EBI-7180728,EBI-1124760
tolAP199345EBI-7180728,EBI-1120026

Protein-protein interaction databases

BioGridi4261828. 329 interactors.
DIPiDIP-48262N.
IntActiP0A855. 11 interactors.
MINTiMINT-7888580.
STRINGi511145.b0740.

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 29Combined sources4
Beta strandi31 – 33Combined sources3
Beta strandi34 – 39Combined sources6
Beta strandi43 – 49Combined sources7
Helixi54 – 64Combined sources11
Beta strandi67 – 70Combined sources4
Helixi73 – 75Combined sources3
Helixi83 – 85Combined sources3
Helixi88 – 92Combined sources5
Turni93 – 95Combined sources3
Beta strandi98 – 106Combined sources9
Beta strandi112 – 120Combined sources9
Beta strandi122 – 124Combined sources3
Beta strandi128 – 136Combined sources9
Helixi138 – 140Combined sources3
Helixi141 – 157Combined sources17
Beta strandi166 – 172Combined sources7
Beta strandi174 – 177Combined sources4
Beta strandi180 – 186Combined sources7
Beta strandi193 – 200Combined sources8
Beta strandi202 – 207Combined sources6
Beta strandi211 – 218Combined sources8
Beta strandi225 – 230Combined sources6
Turni231 – 233Combined sources3
Beta strandi236 – 240Combined sources5
Beta strandi246 – 251Combined sources6
Beta strandi255 – 262Combined sources8
Beta strandi269 – 274Combined sources6
Turni275 – 277Combined sources3
Beta strandi280 – 282Combined sources3
Beta strandi286 – 288Combined sources3
Beta strandi290 – 295Combined sources6
Beta strandi299 – 306Combined sources8
Beta strandi313 – 318Combined sources6
Beta strandi330 – 339Combined sources10
Beta strandi343 – 351Combined sources9
Beta strandi356 – 362Combined sources7
Turni363 – 365Combined sources3
Beta strandi368 – 370Combined sources3
Beta strandi374 – 376Combined sources3
Beta strandi378 – 382Combined sources5
Beta strandi386 – 395Combined sources10
Beta strandi398 – 405Combined sources8
Beta strandi411 – 413Combined sources3
Beta strandi417 – 426Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C5KX-ray2.00A1-430[»]
1CRZX-ray1.95A28-430[»]
2HQSX-ray1.50A/B/D/F23-430[»]
2IVZX-ray2.00A/B/C/D1-430[»]
2W8BX-ray1.86A/B/D/F22-430[»]
3IAXX-ray2.60A1-430[»]
ProteinModelPortaliP0A855.
SMRiP0A855.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A855.

Family & Domainsi

Sequence similaritiesi

Belongs to the TolB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107R6Y. Bacteria.
COG0823. LUCA.
HOGENOMiHOG000004098.
InParanoidiP0A855.
KOiK03641.
OMAiVREPSWG.
PhylomeDBiP0A855.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.40.50.10070. 1 hit.
HAMAPiMF_00671. TolB. 1 hit.
InterProiIPR011659. PD40.
IPR014167. Tol-Pal_propeller_TolB.
IPR007195. TolB_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF07676. PD40. 4 hits.
PF04052. TolB_N. 1 hit.
[Graphical view]
SUPFAMiSSF52964. SSF52964. 1 hit.
TIGRFAMsiTIGR02800. propeller_TolB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA
60 70 80 90 100
PEDIGGIVAA DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV
110 120 130 140 150
VGQVTPNPDG SYNVAYQLVD TGGAPGTVLA QNSYKVNKQW LRYAGHTASD
160 170 180 190 200
EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY ELRVSDYDGY NQFVVHRSPQ
210 220 230 240 250
PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA SFPRHNGAPA
260 270 280 290 300
FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ
310 320 330 340 350
NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS
360 370 380 390 400
SNGGQQHIAK QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV
410 420 430
LNLVSTDGRF KARLPATDGQ VKFPAWSPYL
Length:430
Mass (Da):45,956
Last modified:June 7, 2005 - v1
Checksum:i508795C6E6886B52
GO

Sequence cautioni

The sequence AAA24684 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24684.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73834.1.
AP009048 Genomic DNA. Translation: BAA35406.2.
X65796 Genomic DNA. Translation: CAA46672.1.
PIRiC64810.
RefSeqiNP_415268.1. NC_000913.3.
WP_001295307.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740.
BAA35406; BAA35406; BAA35406.
GeneIDi945429.
KEGGiecj:JW5100.
eco:b0740.
PATRICi32116679. VBIEscCol129921_0772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA. Translation: AAA24684.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73834.1.
AP009048 Genomic DNA. Translation: BAA35406.2.
X65796 Genomic DNA. Translation: CAA46672.1.
PIRiC64810.
RefSeqiNP_415268.1. NC_000913.3.
WP_001295307.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C5KX-ray2.00A1-430[»]
1CRZX-ray1.95A28-430[»]
2HQSX-ray1.50A/B/D/F23-430[»]
2IVZX-ray2.00A/B/C/D1-430[»]
2W8BX-ray1.86A/B/D/F22-430[»]
3IAXX-ray2.60A1-430[»]
ProteinModelPortaliP0A855.
SMRiP0A855.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261828. 329 interactors.
DIPiDIP-48262N.
IntActiP0A855. 11 interactors.
MINTiMINT-7888580.
STRINGi511145.b0740.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

2D gel databases

SWISS-2DPAGEP0A855.

Proteomic databases

EPDiP0A855.
PaxDbiP0A855.
PRIDEiP0A855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740.
BAA35406; BAA35406; BAA35406.
GeneIDi945429.
KEGGiecj:JW5100.
eco:b0740.
PATRICi32116679. VBIEscCol129921_0772.

Organism-specific databases

EchoBASEiEB1001.
EcoGeneiEG11008. tolB.

Phylogenomic databases

eggNOGiENOG4107R6Y. Bacteria.
COG0823. LUCA.
HOGENOMiHOG000004098.
InParanoidiP0A855.
KOiK03641.
OMAiVREPSWG.
PhylomeDBiP0A855.

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER.
ECOL316407:JW5100-MONOMER.
MetaCyc:EG11008-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A855.
PROiP0A855.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.40.50.10070. 1 hit.
HAMAPiMF_00671. TolB. 1 hit.
InterProiIPR011659. PD40.
IPR014167. Tol-Pal_propeller_TolB.
IPR007195. TolB_N.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF07676. PD40. 4 hits.
PF04052. TolB_N. 1 hit.
[Graphical view]
SUPFAMiSSF52964. SSF52964. 1 hit.
TIGRFAMsiTIGR02800. propeller_TolB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOLB_ECOLI
AccessioniPrimary (citable) accession number: P0A855
Secondary accession number(s): P19935
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.