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Protein

Tol-Pal system protein TolB

Gene

tolB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity (PubMed:17233825). TolB occupies a key intermediary position in the Tol-Pal system because it communicates directly with both membrane-embedded components, Pal in the outer membrane and TolA in the inner membrane (PubMed:19696740). Is also involved in the uptake of some colicins A (PubMed:2687247, PubMed:7929011, PubMed:11994151). The Tol-Pal system is also required for polar localization of chemoreceptors clusters (PubMed:24720726).2 Publications4 Publications

GO - Molecular functioni

  • protein-containing complex binding Source: CAFA
  • protein domain specific binding Source: CAFA

GO - Biological processi

  • bacteriocin transport Source: EcoliWiki
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cellular response to bacteriocin Source: EcoCyc
  • protein import Source: EcoliWiki
  • protein transport Source: EcoliWiki

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG11008-MONOMER
MetaCyc:EG11008-MONOMER

Protein family/group databases

TCDBi2.C.1.2.1 the tonb-exbb-exbd/tola-tolq-tolr outer membrane receptor energizers and stabilizers (tonb/tola) family

Names & Taxonomyi

Protein namesi
Recommended name:
Tol-Pal system protein TolBUniRule annotationCurated
Gene namesi
Name:tolB1 PublicationUniRule annotation
Ordered Locus Names:b0740, JW5100
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11008 tolB

Subcellular locationi

  • Periplasm UniRule annotation2 Publications
  • Note: Partially associated with the outer membrane through a specific interaction with Pal (PubMed:7744736). Accumulates at cell constriction sites. Recruitment to the division site is dependent on FtsN activity (PubMed:17233825).2 Publications

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
  • periplasmic space Source: EcoliWiki
  • protein-containing complex Source: CAFA

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Mutants lacking the tol-pal cluster suffer delayed outer membrane invagination and contain large outer membrane blebs at constriction sites and cell poles.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21UniRule annotation2 PublicationsAdd BLAST21
ChainiPRO_000003464722 – 430Tol-Pal system protein TolBUniRule annotationAdd BLAST409

Proteomic databases

EPDiP0A855
PaxDbiP0A855
PRIDEiP0A855

2D gel databases

SWISS-2DPAGEP0A855

Interactioni

Subunit structurei

The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer (PubMed:17233825). TolB forms homodimers (PubMed:11994151). Interacts with the peptidoglycan-associated lipoprotein Pal and with the C-terminal region of TolA (PubMed:7744736, PubMed:11994151, PubMed:17375930, PubMed:19696740). Interacts with the trimeric porins OmpF, OmpC, PhoE and LamB (PubMed:9393690). Can form a complex with Pal, Lpp and OmpA (PubMed:9701827). Interacts with colicin E9 (PubMed:10673426, PubMed:16894158).1 Publication8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi4261828, 397 interactors
DIPiDIP-48262N
IntActiP0A855, 11 interactors
MINTiP0A855
STRINGi316385.ECDH10B_0807

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 29Combined sources4
Beta strandi31 – 33Combined sources3
Beta strandi34 – 39Combined sources6
Beta strandi43 – 49Combined sources7
Helixi54 – 64Combined sources11
Beta strandi67 – 70Combined sources4
Helixi73 – 75Combined sources3
Helixi83 – 85Combined sources3
Helixi88 – 92Combined sources5
Turni93 – 95Combined sources3
Beta strandi98 – 106Combined sources9
Beta strandi112 – 120Combined sources9
Beta strandi122 – 124Combined sources3
Beta strandi128 – 136Combined sources9
Helixi138 – 140Combined sources3
Helixi141 – 157Combined sources17
Beta strandi166 – 172Combined sources7
Beta strandi174 – 177Combined sources4
Beta strandi180 – 186Combined sources7
Beta strandi193 – 200Combined sources8
Beta strandi202 – 207Combined sources6
Beta strandi211 – 218Combined sources8
Beta strandi225 – 230Combined sources6
Turni231 – 233Combined sources3
Beta strandi236 – 240Combined sources5
Beta strandi246 – 251Combined sources6
Beta strandi255 – 262Combined sources8
Beta strandi269 – 274Combined sources6
Turni275 – 277Combined sources3
Beta strandi280 – 282Combined sources3
Beta strandi286 – 288Combined sources3
Beta strandi290 – 295Combined sources6
Beta strandi299 – 306Combined sources8
Beta strandi313 – 318Combined sources6
Beta strandi330 – 339Combined sources10
Beta strandi343 – 351Combined sources9
Beta strandi356 – 362Combined sources7
Turni363 – 365Combined sources3
Beta strandi368 – 370Combined sources3
Beta strandi374 – 376Combined sources3
Beta strandi378 – 382Combined sources5
Beta strandi386 – 395Combined sources10
Beta strandi398 – 405Combined sources8
Beta strandi411 – 413Combined sources3
Beta strandi417 – 426Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C5KX-ray2.00A1-430[»]
1CRZX-ray1.95A28-430[»]
2HQSX-ray1.50A/B/D/F23-430[»]
2IVZX-ray2.00A/B/C/D1-430[»]
2W8BX-ray1.86A/B/D/F22-430[»]
3IAXX-ray2.60A1-430[»]
ProteinModelPortaliP0A855
SMRiP0A855
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A855

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 34TolA box1 PublicationAdd BLAST13

Domaini

Effector proteins that bind to TolB can both negatively (Pal) and positively (colicin E9) regulate association of the distal N-terminal TolA box with TolA by influencing the conformational equilibrium experienced by these residues.1 Publication

Sequence similaritiesi

Belongs to the TolB family.UniRule annotationCurated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107R6Y Bacteria
COG0823 LUCA
HOGENOMiHOG000004098
InParanoidiP0A855
KOiK03641
OMAiVREPSWG
PhylomeDBiP0A855

Family and domain databases

Gene3Di2.120.10.30, 1 hit
3.40.50.10070, 1 hit
HAMAPiMF_00671 TolB, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR011659 PD40
IPR014167 Tol-Pal_TolB
IPR007195 TolB_N
IPR036752 TolB_N_sf
PfamiView protein in Pfam
PF07676 PD40, 4 hits
PF04052 TolB_N, 1 hit
TIGRFAMsiTIGR02800 propeller_TolB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA
60 70 80 90 100
PEDIGGIVAA DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV
110 120 130 140 150
VGQVTPNPDG SYNVAYQLVD TGGAPGTVLA QNSYKVNKQW LRYAGHTASD
160 170 180 190 200
EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY ELRVSDYDGY NQFVVHRSPQ
210 220 230 240 250
PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA SFPRHNGAPA
260 270 280 290 300
FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ
310 320 330 340 350
NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS
360 370 380 390 400
SNGGQQHIAK QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV
410 420 430
LNLVSTDGRF KARLPATDGQ VKFPAWSPYL
Length:430
Mass (Da):45,956
Last modified:June 7, 2005 - v1
Checksum:i508795C6E6886B52
GO

Sequence cautioni

The sequence AAA24684 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28232 Genomic DNA Translation: AAA24684.1 Different initiation.
U00096 Genomic DNA Translation: AAC73834.1
AP009048 Genomic DNA Translation: BAA35406.2
X65796 Genomic DNA Translation: CAA46672.1
PIRiC64810
RefSeqiNP_415268.1, NC_000913.3
WP_001295307.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73834; AAC73834; b0740
BAA35406; BAA35406; BAA35406
GeneIDi945429
KEGGiecj:JW5100
eco:b0740
PATRICifig|1411691.4.peg.1532

Similar proteinsi

Entry informationi

Entry nameiTOLB_ECOLI
AccessioniPrimary (citable) accession number: P0A855
Secondary accession number(s): P19935
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health