Reviewed, UniProtKB/Swiss-Prot P0A855 (TOLB_ECOLI)

Names and origin

Protein names

Recommended name:
    Protein tolB

Gene names

Name:	tolB
Ordered Locus Names:	b0740, JW5100

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. HAMAP MF_00671
Subcellular location	Periplasm. Ref.6
Sequence similarities	Belongs to the tolB family.

Ontologies

Keywords
Biological process	Bacteriocin transport Protein transport Transport
Cellular component	Periplasm
Domain	Signal
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	bacteriocin transport Inferred from electronic annotation. Source: UniProtKB-KW protein import Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.6 Ref.7

Chain

22 – 430

409

Protein tolB HAMAP MF_00671

PRO_0000034647

Secondary structure

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430

Helix Strand Turn

Details...

Beta strand

31 – 33

3

Beta strand

35 – 39

5

Beta strand

46 – 49

4

Helix

54 – 64

11

Beta strand

66 – 70

5

Helix

73 – 75

3

Turn

83 – 85

3

Helix

88 – 91

4

Turn

92 – 95

4

Beta strand

99 – 106

8

Beta strand

112 – 120

9

Beta strand

122 – 124

3

Beta strand

127 – 136

10

Helix

138 – 140

3

Helix

141 – 157

17

Beta strand

166 – 172

7

Beta strand

176 – 178

3

Beta strand

180 – 186

7

Beta strand

193 – 200

8

Beta strand

202 – 207

6

Beta strand

211 – 218

8

Beta strand

225 – 230

6

Turn

231 – 233

3

Beta strand

236 – 240

5

Beta strand

246 – 251

6

Beta strand

255 – 262

8

Beta strand

269 – 274

6

Turn

275 – 277

3

Beta strand

280 – 282

3

Beta strand

286 – 288

3

Beta strand

290 – 295

6

Beta strand

299 – 306

8

Beta strand

313 – 318

6

Beta strand

330 – 339

10

Beta strand

343 – 352

10

Beta strand

355 – 362

8

Turn

363 – 365

3

Beta strand

368 – 370

3

Beta strand

374 – 376

3

Beta strand

378 – 382

5

Beta strand

386 – 395

10

Beta strand

398 – 405

8

Beta strand

411 – 413

3

Beta strand

418 – 426

9

Sequences

	Sequence		Length	Mass (Da)
	P0A855-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 508795C6E6886B52	FASTA	430	45,956
	10 20 30 40 50 60 MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA PEDIGGIVAA 70 80 90 100 110 120 DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV VGQVTPNPDG SYNVAYQLVD 130 140 150 160 170 180 TGGAPGTVLA QNSYKVNKQW LRYAGHTASD EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY 190 200 210 220 230 240 ELRVSDYDGY NQFVVHRSPQ PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA 250 260 270 280 290 300 SFPRHNGAPA FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ 310 320 330 340 350 360 NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS SNGGQQHIAK 370 380 390 400 410 420 QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV LNLVSTDGRF KARLPATDGQ 430 VKFPAWSPYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli." Levengood S.K., Webster R.E. J. Bacteriol. 171:6600-6609(1989) [PubMed: 2687247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / JM105 / ATCC 47016.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL)." Lazzaroni J.-C., Portalier R. Mol. Microbiol. 6:735-742(1992) [PubMed: 1574003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 419-430. Strain: K12.
[6]	"Maturation and localization of the TolB protein required for colicin import." Isnard M., Rigal A., Lazzaroni J.-C., Lazdunski C., Lloubes R. J. Bacteriol. 176:6392-6396(1994) [PubMed: 7929011] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-27, SUBCELLULAR LOCATION.
[7]	Frutiger S., Hughes G.J., Pasquali C., Hochstrasser D.F. Submitted (FEB-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 22-32. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Crystallization and preliminary crystallographic study of a component of the Escherichia coli Tol system: TolB." Abergel C., Rigal A., Chenivesse S., Lazdunski C., Claverie J.-M., Bouveret E., Benedetti H. Acta Crystallogr. D 54:102-104(1998) [PubMed: 9761825] [Abstract] Cited for: CRYSTALLIZATION.
[9]	"Structure of the Escherichia coli TolB protein determined by MAD methods at 1.95-A resolution." Abergel C., Bouveret E., Claverie J.-M., Brown K., Rigal A., Lazdunski C., Benedetti H. Structure 7:1291-1300(1999) [PubMed: 10545334] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M28232 Genomic DNA. Translation: AAA24684.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73834.1.
AP009048 Genomic DNA. Translation: BAA35406.2.
X65796 Genomic DNA. Translation: CAA46672.1.

PIR

C64810.

RefSeq

AP_001378.1.
NP_415268.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1C5K	X-ray	2.00	A	1-430	[»]
1CRZ	X-ray	1.95	A	28-430	[»]
2HQS	X-ray	1.50	A/B/D/F	23-430	[»]
2IVZ	X-ray	2.00	A/B/C/D	1-430	[»]
2W8B	X-ray	1.86	A/B/D/F	22-430	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P0A855.

Protein family/group databases

TCDB

2.C.1.2.1. TonB-ExbB-ExbD/TolA-TolQ-TolR (TonB) family of auxiliary proteins for energization of outer membrane receptor (OMR)-mediated active transport.

2-D gel databases

SWISS-2DPAGE

P0A855.

2DBase-Ecoli

P0A855.

Genome annotation databases

GeneID

945429.

GenomeReviews

Gene locus JW5100 in contig AP009048_GR.
Gene locus b0740 in contig U00096_GR.

KEGG

ecj:JW5100.
eco:b0740.

Organism-specific databases

EchoBASE

EB1001.

EcoGene

EG11008. tolB.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A855.

OMA

FATRLSY.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11008-MON.

Gene expression databases

Genevestigator

P0A855.

Family and domain databases

HAMAP

MF_00671.
[Tree]

InterPro

IPR011042. 6-blade_b-propeller_TolB-like.
IPR011659. PD40.
IPR014167. Tol-Pal_propeller_TolB.
IPR007195. TolB_N.
[Graphical view]

Gene3D

G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
G3DSA:3.40.50.10070. TolB_N. 1 hit.

Pfam

PF07676. PD40. 5 hits.
PF04052. TolB_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02800. propeller_TolB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

TOLB_ECOLI

Accession

Primary (citable) accession number: P0A855
Secondary accession number(s): P19935

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)