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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Nucleophile
Binding sitei90 – 901Substrate
Metal bindingi302 – 3021Zinc
Metal bindingi304 – 3041Zinc
Metal bindingi307 – 3071Zinc
Metal bindingi333 – 3331ZincBy similarity

GO - Molecular functioni

  • queuine tRNA-ribosyltransferase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • queuosine biosynthetic process Source: EcoCyc
  • tRNA wobble guanine modification Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciECOL316407:JW0396-MONOMER.
BRENDAi2.4.2.29. 2026.
SABIO-RKP0A847.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferase (EC:2.4.2.29)
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene namesi
Name:tgt
Ordered Locus Names:b0406, JW0396
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10996. tgt.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi28 – 281C → A: Slight loss of activity. 1 Publication
Mutagenesisi90 – 901S → A: Loss of activity. 1 Publication
Mutagenesisi90 – 901S → C: Loss of activity. 1 Publication
Mutagenesisi90 – 901S → F: Loss of activity. 1 Publication
Mutagenesisi145 – 1451C → A: No loss of activity. 1 Publication
Mutagenesisi232 – 2321C → A: Slight loss of activity. 1 Publication
Mutagenesisi265 – 2651C → A: Significant loss of activity. 1 Publication
Mutagenesisi302 – 3021C → A: Loss of activity. 1 Publication
Mutagenesisi304 – 3041C → A: Loss of activity. 1 Publication
Mutagenesisi307 – 3071C → A: Loss of activity. 1 Publication
Mutagenesisi316 – 3161H → A: Slight loss of activity. 1 Publication
Mutagenesisi317 – 3171H → A: Loss of activity. 1 Publication
Mutagenesisi321 – 3211C → A: No loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Queuine tRNA-ribosyltransferasePRO_0000135471Add
BLAST

Proteomic databases

EPDiP0A847.
PaxDbiP0A847.
PRIDEiP0A847.

Interactioni

Subunit structurei

Homotrimer or homodimer.

Protein-protein interaction databases

BioGridi4263531. 9 interactions.
DIPiDIP-36020N.
IntActiP0A847. 39 interactions.
MINTiMINT-1219658.
STRINGi511145.b0406.

Structurei

3D structure databases

ProteinModelPortaliP0A847.
SMRiP0A847. Positions 3-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
InParanoidiP0A847.
KOiK00773.
OMAiGIDLFDC.
PhylomeDBiP0A847.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFELDTTDG RARRGRLVFD RGVVETPCFM PVGTYGTVKG MTPEEVEATG
60 70 80 90 100
AQIILGNTFH LWLRPGQEIM KLHGDLHDFM QWKGPILTDS GGFQVFSLGD
110 120 130 140 150
IRKITEQGVH FRNPINGDPI FLDPEKSMEI QYDLGSDIVM IFDECTPYPA
160 170 180 190 200
DWDYAKRSME MSLRWAKRSR ERFDSLGNKN ALFGIIQGSV YEDLRDISVK
210 220 230 240 250
GLVDIGFDGY AVGGLAVGEP KADMHRILEH VCPQIPADKP RYLMGVGKPE
260 270 280 290 300
DLVEGVRRGI DMFDCVMPTR NARNGHLFVT DGVVKIRNAK YKSDTGPLDP
310 320 330 340 350
ECDCYTCRNY SRAYLHHLDR CNEILGARLN TIHNLRYYQR LMAGLRKAIE
360 370
EGKLESFVTD FYQRQGREVP PLNVD
Length:375
Mass (Da):42,594
Last modified:June 7, 2005 - v1
Checksum:i40271CA08D8A8820
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 552IL → MV (PubMed:1706703).Curated
Sequence conflicti54 – 552IL → MV (PubMed:8323579).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63939 Genomic DNA. Translation: AAA24667.1.
U00096 Genomic DNA. Translation: AAC73509.1.
AP009048 Genomic DNA. Translation: BAE76186.1.
U82664 Genomic DNA. Translation: AAB40162.1.
X56175 Genomic DNA. Translation: CAA39631.1. Sequence problems.
X56175 Genomic DNA. Translation: CAA39632.1. Sequence problems.
S68715 Genomic DNA. Translation: AAC60467.2.
PIRiC38530.
RefSeqiNP_414940.1. NC_000913.3.
WP_000667319.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73509; AAC73509; b0406.
BAE76186; BAE76186; BAE76186.
GeneIDi949130.
KEGGiecj:JW0396.
eco:b0406.
PATRICi32115961. VBIEscCol129921_0422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63939 Genomic DNA. Translation: AAA24667.1.
U00096 Genomic DNA. Translation: AAC73509.1.
AP009048 Genomic DNA. Translation: BAE76186.1.
U82664 Genomic DNA. Translation: AAB40162.1.
X56175 Genomic DNA. Translation: CAA39631.1. Sequence problems.
X56175 Genomic DNA. Translation: CAA39632.1. Sequence problems.
S68715 Genomic DNA. Translation: AAC60467.2.
PIRiC38530.
RefSeqiNP_414940.1. NC_000913.3.
WP_000667319.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A847.
SMRiP0A847. Positions 3-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263531. 9 interactions.
DIPiDIP-36020N.
IntActiP0A847. 39 interactions.
MINTiMINT-1219658.
STRINGi511145.b0406.

Proteomic databases

EPDiP0A847.
PaxDbiP0A847.
PRIDEiP0A847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73509; AAC73509; b0406.
BAE76186; BAE76186; BAE76186.
GeneIDi949130.
KEGGiecj:JW0396.
eco:b0406.
PATRICi32115961. VBIEscCol129921_0422.

Organism-specific databases

EchoBASEiEB0989.
EcoGeneiEG10996. tgt.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
InParanoidiP0A847.
KOiK00773.
OMAiGIDLFDC.
PhylomeDBiP0A847.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciECOL316407:JW0396-MONOMER.
BRENDAi2.4.2.29. 2026.
SABIO-RKP0A847.

Miscellaneous databases

PROiP0A847.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt. 1 hit.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTGT_ECOLI
AccessioniPrimary (citable) accession number: P0A847
Secondary accession number(s): P19675
, P19676, P78226, Q2MC20, Q47627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.