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Protein

SsrA-binding protein

Gene

smpB

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.UniRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SsrA-binding proteinUniRule annotation
Alternative name(s):
Small protein BUniRule annotation
Gene namesi
Name:smpBUniRule annotation
Synonyms:smqB
Ordered Locus Names:SF2679, S2857
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Note: The tmRNA-SmpB complex associates with stalled 70S ribosomes.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001030252 – 160SsrA-binding proteinAdd BLAST159

Proteomic databases

PRIDEiP0A835

Structurei

3D structure databases

ProteinModelPortaliP0A835
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SmpB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UH4 Bacteria
COG0691 LUCA
HOGENOMiHOG000009628
KOiK03664
OMAiKLHDKRA

Family and domain databases

CDDicd09294 SmpB, 1 hit
Gene3Di2.40.280.10, 1 hit
HAMAPiMF_00023 SmpB, 1 hit
InterProiView protein in InterPro
IPR023620 SmpB
IPR000037 SsrA-bd_prot
IPR020081 SsrA-bd_prot_CS
PANTHERiPTHR30308 PTHR30308, 1 hit
PfamiView protein in Pfam
PF01668 SmpB, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004488 SmpB, 1 hit
SUPFAMiSSF74982 SSF74982, 1 hit
TIGRFAMsiTIGR00086 smpB, 1 hit
PROSITEiView protein in PROSITE
PS01317 SSRP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKKAHKPG SATIALNKRA RHEYFIEEEF EAGLALQGWE VKSLRAGKAN
60 70 80 90 100
ISDSYVLLRD GEAFLFGANI TPMAVASTHV VCDPTRTRKL LLNQRELDSL
110 120 130 140 150
YGRVNREGYT VVALSLYWKN AWCKVKIGVA KGKKQHDKRS DIKEREWQVD
160
KARIMKNAHR
Length:160
Mass (Da):18,269
Last modified:January 23, 2007 - v2
Checksum:iF7E45A16540EA300
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN44174.1
AE014073 Genomic DNA Translation: AAP17999.1
RefSeqiNP_708467.1, NC_004337.2
WP_000162574.1, NZ_NIYV01000110.1

Genome annotation databases

EnsemblBacteriaiAAN44174; AAN44174; SF2679
AAP17999; AAP17999; S2857
GeneIDi1027251
KEGGisfl:SF2679
sfx:S2857
PATRICifig|198214.7.peg.3189

Similar proteinsi

Entry informationi

Entry nameiSSRP_SHIFL
AccessioniPrimary (citable) accession number: P0A835
Secondary accession number(s): P32052, P77011
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health