Reviewed, UniProtKB/Swiss-Prot P0A832 (SSRP_ECOLI)

Names and origin

Protein names

Recommended name:
    SsrA-binding protein
Alternative name(s):
    Small protein B

Gene names

Name:	smpB
Synonyms:	smqB
Ordered Locus Names:	b2620, JW2601

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	160 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes. HAMAP MF_00023
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the smpB family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.6
	Chain	2 – 160	159	SsrA-binding protein HAMAP MF_00023		PRO_0000102944

Sequences

	Sequence		Length	Mass (Da)
	P0A832-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F7E45A16540EA300	FASTA	160	18,269
	10 20 30 40 50 60 MTKKKAHKPG SATIALNKRA RHEYFIEEEF EAGLALQGWE VKSLRAGKAN ISDSYVLLRD 70 80 90 100 110 120 GEAFLFGANI TPMAVASTHV VCDPTRTRKL LLNQRELDSL YGRVNREGYT VVALSLYWKN 130 140 150 160 AWCKVKIGVA KGKKQHDKRS DIKEREWQVD KARIMKNAHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli." Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H. Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994) [PubMed: 7524073] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Two new genes located between 2758 and 2761 kilobase pairs on the Escherichia coli genome." Chauhan A.K., Miczak A., Apirion D. J. Bacteriol. 173:3271-3272(1991) [PubMed: 2045357] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE OF 2-16. Strain: K12.
[6]	"SmpB, a unique RNA-binding protein essential for the peptide-tagging activity of SsrA (tmRNA)." Karzai A.W., Susskind M.M., Sauer R.T. EMBO J. 18:3793-3799(1999) [PubMed: 10393194] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-8, CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D12501 Genomic DNA. Translation: BAA02062.1. U36840 Genomic DNA. Translation: AAA79790.1. U00096 Genomic DNA. Translation: AAC75669.1. AP009048 Genomic DNA. Translation: BAA16505.2.
PIR	JS0701.
RefSeq	AP_003200.1. NP_417110.1.
3D structure databases
HSSP	HSSP built from PDB template 1P6V based on UniProtKB O66640.
ModBase	Search...
Protein-protein interaction databases
STRING	P0A832.
Genome annotation databases
GeneID	947296.
GenomeReviews	Gene locus JW2601 in contig AP009048_GR. Gene locus b2620 in contig U00096_GR.
KEGG	ecj:JW2601. eco:b2620.
Organism-specific databases
EchoBASE	EB1730.
EcoGene	EG11782. smpB.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A832.
OMA	KLYDKRE.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11782-MON.
Gene expression databases
Genevestigator	P0A832.
Family and domain databases
HAMAP	MF_00023. [Tree]
InterPro	IPR000037. SsrA-bd_prot. IPR020081. SsrA-bd_prot_CS. [Graphical view]
Gene3D	G3DSA:2.40.280.10. SmpB. 1 hit.
Pfam	PF01668. SmpB. 1 hit. [Graphical view]
ProDom	PD004488. SmpB. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00086. smpB. 1 hit.
PROSITE	PS01317. SSRP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSRP_ECOLI

Accession

Primary (citable) accession number: P0A832
Secondary accession number(s): P32052, P77011

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)